LXR beta/NR1H2 Antibody

Western Blot: LXR beta/NR1H2 Antibody [NB100-74457] - 25 ug of rat liver (Lane 1), mouse liver (Lane 2) and Hela (Lane 3) cell lysates.

Immunocytochemistry/Immunofluorescence: LXR beta/NR1H2 Antibody [NB100-74457] - Analysis of LXR beta (green) showing positive staining in the nuclear envelope of C2C12 cells (right) compared with a negative control in the absence of primary antibody (left).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

LXR beta/NR1H2 Antibody Summary

• Description: Novus Biologicals Rabbit LXR beta/NR1H2 Antibody (NB100-74457) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-LXR beta/NR1H2 Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to residues R(113) Y A C R G S G T C Q M D A(126) of rat LXR beta.
• Localization: Nuclear
• Specificity: Detects LXR beta in rat and mouse samples.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NR1H2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Western Blot 1:200 - 1:2000
• Application Notes: WB: Detects an approx. 51 kDa protein representing LXR beta in rat and mouse liver samples.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LXR beta/NR1H2 Antibody

• Liver X receptor beta
• LXR beta
• LXR-b
• NER-I
• NERliver X receptor-beta
• NR1H2
• nuclear orphan receptor LXR-beta
• Nuclear receptor NER
• Nuclear receptor subfamily 1 group H member 2
• nuclear receptor subfamily 1, group H, member 2
• oxysterols receptor LXR-beta
• RIP15
• steroid hormone-nuclear receptor NER
• Ubiquitously-expressed nuclear receptorLXRB
• UNR
• UNRLX receptor beta

Background

Liver X receptors (LXRs), also known as RLD1, are oxysterol activated nuclear receptors which are involved in the regulation of genesis and metabolism of cholesterols and bile acids. LXRs form heterodimeric complexes with the retinoic acid receptors (RARs) once activated by their respective oxysterol ligands. Two known isoforms, alpha and beta, are differentially expressed; the alpha isoform predominantly expressed in liver, whereas beta isoform expression has been shown to be ubiquitous. The corresponding gene for the LXR beta is NR1H2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LXR beta/NR1H2 Antibody (NB100-74457)(7)

Publications using NB100-74457

• Guo H, Zhang Y, Brockman DA et al. Lipocalin 2 Deficiency Alters Estradiol Production and Estrogen Receptor Signaling in Female Mice. Endocrinology. 2012-01-10 [PMID: 22234464]
• De La Fuente D, Tamburini C, Stonelake E et al. Oxysterol-liver X receptor signaling mediates CYFIP1 regulation of cortical neurogenesis bioRxiv 2023-06-26 (WB, Human)
• Daniel Cabezas De La Fuente, Claudia Tamburini, Emily Stonelake, Robert Andrews, Jeremy Hall, Michael J Owen, David E J Linden, Andrew Pocklington, Meng Li Impaired oxysterol-liver X receptor signaling underlies aberrant cortical neurogenesis in a stem cell model of neurodevelopmental disorder. Cell reports 2024-04-01 [PMID: 38483902]
• Wang JQ, Li LL, Hu A Et al. Inhibition of ASGR1 decreases lipid levels by promoting cholesterol excretion Nature 2022-08-03 [PMID: 35922515] (WB)
  
  Details:
  Citation using the FITC version of this antibody.
• Rudalska R, Harbig J, Snaebjornsson M et al. LXR alpha activation and Raf inhibition trigger lethal lipotoxicity in liver cancer Nature Cancer 2021-02-01 [PMID: 35122079]
• Banno A, Wang J, Okada K Identification of a novel cholesterol-lowering dipeptide, phenylalanine-proline (FP), and its down-regulation of intestinal ABCA1 in hypercholesterolemic rats and Caco-2 cells Sci Rep 2019-12-19 [PMID: 31857643] (WB, Rat)
• Chen S, Wang JM, Irwin RW et al. Allopregnanolone Promotes Regeneration and Reduces [beta]-Amyloid Burden in a Preclinical Model of Alzheimer’s Disease. PLoS ONE;6(8). 2011-01-01 [PMID: 21918687]

FAQs for LXR beta/NR1H2 Antibody (NB100-74457). (Showing 1 - 1 of 1 FAQs).

1. Could you let us know the buffer formulation of the following product: #NB100-74457
   • Our LXR beta antibody NB100-74457 is provided in the following buffer : PBS with 1mg/ml BSA and 0.05% Sodium Azide

Bioinformatics

• Gene Symbol: NR1H2
• Entrez:
  • 7376(Human)
  • 22260(Mouse)
  • 58851(Rat)
• Uniprot:
  • P55055(Human)