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LXR beta/NR1H2 Antibody

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Western Blot: LXR beta/NR1H2 Antibody [NB100-74457] - 25 ug of rat liver (Lane 1), mouse liver (Lane 2) and Hela (Lane 3) cell lysates.
Western Blot: LXR beta/NR1H2 Antibody [NB100-74457] - 25 ug of rat liver (Lane 1), mouse liver (Lane 2) and Hela (Lane 3) cell lysates.
Immunocytochemistry/ Immunofluorescence: LXR beta/NR1H2 Antibody [NB100-74457] - Analysis of LXR beta (green) showing positive staining in the nuclear envelope of C2C12 cells (right) compared with a negative control in ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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LXR beta/NR1H2 Antibody Summary

Immunogen
Synthetic peptide corresponding to residues R(113) Y A C R G S G T C Q M D A(126) of rat LXR beta.
Localization
Nuclear
Specificity
Detects LXR beta in rat and mouse samples.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NR1H2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Western Blot 1:200 - 1:2000
Application Notes
WB: Detects an approx. 51 kDa protein representing LXR beta in rat and mouse liver samples.
Publications
Read Publications using
NB100-74457 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LXR beta/NR1H2 Antibody

  • Liver X receptor beta
  • LXR beta
  • LXR-b
  • NER-I
  • NERliver X receptor-beta
  • NR1H2
  • nuclear orphan receptor LXR-beta
  • Nuclear receptor NER
  • Nuclear receptor subfamily 1 group H member 2
  • nuclear receptor subfamily 1, group H, member 2
  • oxysterols receptor LXR-beta
  • RIP15
  • steroid hormone-nuclear receptor NER
  • Ubiquitously-expressed nuclear receptorLXRB
  • UNR
  • UNRLX receptor beta

Background

Liver X receptors (LXRs), also known as RLD1, are oxysterol activated nuclear receptors which are involved in the regulation of genesis and metabolism of cholesterols and bile acids. LXRs form heterodimeric complexes with the retinoic acid receptors (RARs) once activated by their respective oxysterol ligands. Two known isoforms, alpha and beta, are differentially expressed; the alpha isoform predominantly expressed in liver, whereas beta isoform expression has been shown to be ubiquitous. The corresponding gene for the LXR beta is NR1H2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: XPB Antibody [NB100-61060] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiERCC3 antibody used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
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Western Blot: XPC Antibody (3.26) [NB100-477] - Whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with XPC antibody [3.26] diluted at 1:500.
Immunocytochemistry/Immunofluorescence: XPC Antibody (3.26) [NB100-477] - Left: HeLa cells stained with XPC (3.26) monoclonal antibody (NB100-477). Right: DAPI Staining Photo is kindly provided by Dr. S. Liu at U of Arizona.
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Immunohistochemistry: hHR23b Antibody (7G10) [NBP2-45514] - Analysis of Carcinoma of Human kidney tissue.. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: hHR23b Antibody (7G10) [NBP2-45514] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Species: Hu, Mu, Rt
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Western Blot: CSH2 Antibody [NBP2-16019] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: CSH2 Antibody [NBP2-16019] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.
Species: Hu
Applications: IHC, IHC-P, WB
XPE/DDB2 Antibody
AF3297
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human XPE/DDB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3297) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPE/DDB2 at approximately 45 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
(3 Publications)
Species: Hu
Applications: WB

Publications for LXR beta/NR1H2 Antibody (NB100-74457)(3)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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FAQs for LXR beta/NR1H2 Antibody (NB100-74457). (Showing 1 - 1 of 1 FAQs).

  1. Could you let us know the buffer formulation of the following product: #NB100-74457
    • Our LXR beta antibody NB100-74457 is provided in the following buffer : PBS with 1mg/ml BSA and 0.05% Sodium Azide

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Bioinformatics

Gene Symbol NR1H2
Entrez
Uniprot