PKM2 Antibody - BSA Free


Biological Strategies: Western Blot: PKM2 Antibody [NBP1-48308] - Expression of PKM1 and PKM2 in clinical colorectal cancer samples. The protein expression of PKM1 and PKM2 in clinical specimens of cancer tumor more
Biological Strategies: Western Blot: PKM2 Antibody [NBP1-48308] - Expression profile of PKM isoforms in tissues from mouse organs. PKM1 and PKM2 were detected by Western blotting in under the same experimental more
Simple Western: PKM2 Antibody [NBP1-48308] - Lane view shows a specific band for PKM2 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * more
Immunocytochemistry/ Immunofluorescence: PKM2 Antibody [NBP1-48308] - Analysis of PKM2 in frozen sections of rat glioblastoma tissue. Image courtsey of anonymous customer review.
Immunohistochemistry: PKM2 Antibody [NBP1-48308] - Staining of PKM2 in mouse liver tissue.
Flow Cytometry: PKM2 Antibody [NBP1-48308] - An intracellular stain was performed on Neuro2a cells with PKM2 Antibody NBP1-48308AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then more
Western Blot: PKM2 Antibody [NBP1-48308] - Analysis of HeLa lysates using NBP1-48308. Image courtesy of Gregg Semenza, (PMID: 21620138).
Western Blot: PKM2 Antibody [NBP1-48308] - Analysis of PKM2 in MCF7 whole cell lysates.
Biological Strategies: Western Blot: PKM2 Antibody [NBP1-48308] - Expression profile of PKM isoforms in various cancer cell lines, control cell lines, and cells in primary culture. PKM1 and PKM2 were detected by more
Immunocytochemistry/ Immunofluorescence: PKM2 Antibody [NBP1-48308] - Analysis of PKM2 in HeLa cells.
Flow Cytometry: PKM2 Antibody [NBP1-48308] - An intracellular stain was performed on HeLa cells with PKM2 Antibody NBP1-48308AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then more

Product Details

Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, Simple Western, Flow, IB, ICC/IF, IHC, IHC-P, IP, KD
BSA Free
1.0 mg/ml
Validated by:

Biological Strategies


Order Details

PKM2 Antibody - BSA Free Summary

A synthetic peptide made to the internal region of human PKM2 protein (within residues 350-450). [Swiss-Prot: P14618]
Cytoplasm. Nucleus. Note: Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry 5 ug/ml
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation
  • Knockdown Validated
  • Simple Western 1:12.5
  • Western Blot 0.5 ug/ml
Application Notes
In Western blot, a band is seen at approx. 60 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in immunoprecipitation reported in scientific literature (PMID: 21620138). Use in immunoblotting reported in scientific literature (PMID: 25416385).
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
HeLa Whole Cell Lysate
MCF-7 Whole Cell Lysate
Control Peptide
PKM2 Peptide (NBP1-48308PEP)
Reviewed Applications
Read 3 Reviews rated 5
NBP1-48308 in the following applications:

Read Publications using
NBP1-48308 in the following applications:

  • 2 publications
  • 1 publication
  • IP
    1 publication
  • WB
    11 publications

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 25416385).

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.1% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for PKM2 Antibody - BSA Free

  • Cytosolic thyroid hormone-binding protein
  • MGC3932
  • OIP3
  • OIP-3
  • OIP3EC
  • Opa-interacting protein 3
  • p58
  • PK, muscle type
  • PK2
  • PK3
  • PK3PKM
  • PKM2
  • Pyruvate kinase 2/3
  • pyruvate kinase isozymes M1/M2
  • Pyruvate kinase muscle isozyme
  • pyruvate kinase, muscle
  • TCB
  • THBP1
  • THBP1p58
  • Thyroid hormone-binding protein 1
  • thyroid hormone-binding protein, cytosolic
  • Tumor M2-PK


PKM2 (pyruvate kinase isozymes M1/M2), an isoform of pyruvate kinase, is a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP and is expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. Other three pyruvate kinases includes PKL (liver and kidney), PKR (erythrocytes) and PKM1, (adult muscle, brain, bladder, and fibroblasts) which exist as stable tetramers, whereas, PKM2 subunits form tetramers as well as dimers, the dimeric form with higher Km value for PEP substrate stays inactive at physiological concentrations of PEP. The ratio between highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. Predominately localized in the cytoplasm, PKM2 translocate to nucleus upon apoptotic stimuli and this translocation mediated cell death is caspase independent, isoform-specific and independent of its enzymatic activity.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
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Applications: Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
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Applications: Flow, ICC/IF, IHC, IHC-P, WB
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Applications: CyTOF-reported, Flow
Species: Hu
Applications: ICC/IF, WB
Species: Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: Block, CyTOF-ready, Flow
Species: Hu, Mu, Rt
Applications: GS, ICC/IF, IHC, IHC-P
Species: Hu, Rt
Applications: IHC, IHC-P, PEP-ELISA, WB
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
Species: Hu
Applications: CyTOF-ready, Flow
Species: Ce, Ch, Dr, Hu, Mu, Rt, Sh, Ze
Applications: Flow, ICC/IF, IHC-FrFl, IHC, IHC-P, Simple Western, WB
Species: Hu, Mu
Applications: CyTOF-ready, Flow, IP, WB

Publications for PKM2 Antibody (NBP1-48308)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Bovine.

We have publications tested in 4 applications: ICC/IF, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NBP1-48308 Applications Species
Zhang H, Wang D et al. Metabolic and Proliferative State of Vascular Adventitial Fibroblasts in Pulmonary Hypertension Is Regulated Through a MicroRNA-124/PTBP1 (Polypyrimidine Tract Binding Protein 1)/Pyruvate Kinase Muscle Axis. Circulation Dec 19 2017 [PMID: 28972001] (WB, Bovine) WB Bovine
Sung WW, Chen PR, Liao MH, Lee JW. Enhanced aerobic glycolysis of nasopharyngeal carcinoma cells by Epstein-Barr virus latent membrane protein 1 Exp. Cell Res. 2017 Aug 04 [PMID: 28827059] (WB, Human) WB Human
Minami K, Taniguchi K, Sugito N et al. MiR-145 negatively regulates Warburg effect by silencing KLF4 and PTBP1 in bladder cancer cells. Oncotarget. 2017 May 16 [PMID: 28380435] (WB, Human) WB Human
Takai T, Yoshikawa Y, Inamoto T et al. A Novel Combination RNAi toward Warburg Effect by Replacement with miR-145 and Silencing of PTBP1 Induces Apoptotic Cell Death in Bladder Cancer Cells Int J Mol Sci Jan 17 2017 [PMID: 28106737] (WB, Human) WB Human
Sugiyama T, Taniguchi K, Matsuhashi N et al. MiR-133b inhibits growth of human gastric cancer cells by silencing pyruvate kinase muscle-splicer polypyrimidine tract-binding protein 1. Cancer Sci. Dec 1 2016 [PMID: 27696637] (WB, Human) WB Human
Christensen DR, Calder PC, Houghton FD. GLUT3 and PKM2 regulate OCT4 expression and support the hypoxic culture of human embryonic stem cells. Sci Rep. 2015 Dec 07 [PMID: 26639784] (WB, ICC/IF, Human) WB, ICC/IF Human
Taniguchi K, Sugito N, Kumazaki M et al. Positive feedback of DDX6/c-Myc/PTB1 regulated by miR-124 contributes to maintenance of the Warburg effect in colon cancer cells Biochim. Biophys. Acta. 2015 Jul 02 [PMID: 26144048] (WB, Human)

PKM2 antibody was used for WB assay on lysates of human colon cancer cells (DLD-1 cells and WiDr cells ) transfected with miR-124 or siR-PTB1 (Fig. 3B and 3C). WB was also performed on lysates after the transfection of colon cancer cells with siR-c-Myc or siR-DDX6 (Fig. 4A qand 4B), and on DLD-1 cells /WiDr cells that were subjected to PKM1 and/or PKM2 knockdown using siRNAPKM1 and siRNAPKM2 (Fig. 5B and 5E).
WB Human
Taniguchi K, Ito Y, Sugito N et al. Organ-specific PTB1-associated microRNAs determine expression of pyruvate kinase isoforms Sci Rep 2015 Feb 27 [PMID: 25721733] (WB, ICC/IF, IHC-P, Mouse, Human) WB, ICC/IF, IHC-P Mouse, Human
Shinohara H, Taniguchi K, Kumazaki M et al. Anti-cancer fatty-acid derivative induces autophagic cell death through modulation of PKM isoform expression profile mediated by bcr-abl in chronic myeloid leukemia Cancer Lett. 2015 Jan 30 [PMID: 25644089] (WB, Human)

PKM2 antibody used for WB on lysates of human leukemia cell lines K562 and KCL-22 cells which were subjected to AIC-47/a fatty-acid derivative treatment for 72?hours (Fig.?3B). WB also performed on the lysates of K562 and KCL-22 cells at 72?h after transfection of the cells with control RNA or siRNAs against c-Myc, PTB1 or PKM2 (Fig.?3C, 3D and 3E ), and with control RNA or bcr-abl-specific siRNA /siR-bcr-abl (Fig.?4). PKM1 WB also performed on lysates of K562 cells transfected with control siRNA or siRNA-STAT3 (Figure S1A) and cells treated or not with Rapamycin (Figure S2).
WB Human
Bruns Dr, Brown Rd, Stenmark Kr et al. Mitochondrial Integrity in a neonatal Bovine Model of Right Ventricular Dysfunction. Am. J. Physiol. Lung Cell Mol. Physiol. 2014 Nov 21 [PMID: 25416385]
Show All 14 Publications.

Reviews for PKM2 Antibody (NBP1-48308) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Rat.

Reviews using NBP1-48308:
Filter by Applications
Western Blot
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
reviewed by:
Verified Customer
Immunocytochemistry Human 06/15/2017


Sample TestedHuman lung cancer cell lines
Western Blot PKM2 NBP1-48308
reviewed by:
Verified Customer
Western Blot Human 02/17/2011


ApplicationWestern Blot
Sample TestedHeLa cells
Immunofluorescence PKM2 NBP1-48308
reviewed by:
Verified Customer
Immunofluorescence Rat 10/15/2010


Sample TestedRat glioblastoma frozen section

Product General Protocols

View specific protocols for PKM2 Antibody (NBP1-48308): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PKM2 Antibody (NBP1-48308). (Showing 1 - 1 of 1 FAQs).

  1. I am wondering if this antibody can be used for IP or ChIP? Have you ever tested it in either of these applications?
    • We have not yet tested this antibody in ChIP or IP. However, if you would like to test this antibody in either of these applications and share your results with us, you would become eligible for our Innovators Reward Program.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

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MAPK3/ERK1 - A signal transduction pathway with roles in development and disease
Mitogen-activated protein kinases (MAPKs) are important signaling proteins needed to transmit and relay extracellular stimuli and to illicit intracellular responses (1). The MAPK family of proteins are serine/threonine kinases that are able to phos...  Read full blog post.

5 Stars for the PKM2 Antibody
Novus' Pyruvate Kinase M2 antibody (cat # NBP1-48308) has received some glowing product reviews and customer feedback lately. One satisfied customer wrote, "The best thing about this antibody is that it works well for immunofluorescence staining of fr...  Read full blog post.

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Recent Reviews


Verified Customer
Application: Immunocytochemistry
Species: Human

Verified Customer
Application: Western Blot
Species: Human

Verified Customer
Application: Immunofluorescence
Species: Rat


Gene Symbol PKM