AHR Antibody - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: AHR Antibody [NB100-128] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti AHR [NB100-128] at a 1:100 dilution overnight at 4C and detected with an anti-goat DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: AHR Antibody [NB100-128] - Analysis of FFPE human liver using 1:200 conc. of AHR antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.

Gel Super Shift Assays: AHR Antibody [NB100-128] - EMSA validation of AHR antibody on U937 cells nuclear protein. Image submitted by verified customer - see review for protocol details.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ChIP, GS, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: Azide and BSA Free

AHR Antibody - Azide and BSA Free Summary

• Immunogen: N-terminal sequence of Aryl hydrocarbon Receptor purified from C57BL/6J mice. [UniProt# P30561]
• Clonality: Polyclonal
• Host: Goat
• Gene: AHR
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation reported in scientific literature (PMID 3999131)
  • Gel Super Shift Assays
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Paraffin 1:100 - 1:200
  • Western Blot reported in scientific literature (PMID 31351871; 24302727; 3999131)
• Application Notes: Suggested working dilution for Gel Super Shift Assay (EMSA) is 4ul of antibody to a 15 ul final volume of EMSA sample. The current lot of this antibody did not work in WB to our QC Team's expectations.
• Control:

  AHR Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Purity: Unpurified

Alternate Names for AHR Antibody - Azide and BSA Free

• Ah receptor
• AHR
• AH-receptor
• aryl hydrocarbon receptor
• BHLHE76
• bHLHe76aromatic hydrocarbon receptor
• Class E basic helix-loop-helix protein 76

Background

Aryl hydrocarbon Receptor (AhR) is a ligand-activated transcriptional activator that binds to XRE promoter region of its target genes and has been established to activate phase I/II xenobiotic biotransformation enzyme genes. AhR binds MYBBP1A and its efficient DNA binding requires dimerization with another bHLH proteinsuch as ARNT. Besides interacting with NEDD8 and IVNS1ABP, AhR also interacts with coactivators including SRC-1, RIP140 and NOCA7, and with the corepressor SMRT. AhR localize to cytoplasm and upon binding with ligand followed by interaction with a HSP90, it translocates to the nucleus. AhR has been shown to be induced or repressed by TGFB1 as well as dioxin in a cell-type specific fashion, whereas cAMP, retinoic acid, TPA (12-O-tetradecanoyl phorbol-13 acetate) have been shown to exert repressive effects on AhR. As a member of bHLH-PAS (basic helix-loop-helix Per/ARNT/Sim) family, AhR has been implicated in physiological processes such as circadian cycle, neurogenesis, organs development and regulation of cell-cycle as well as the alternative signaling pathways (independently of exposure to xenobiotics). AhR-null mice exhibit infertility, liver abnormalities and cardiovascular problems such as a defective closure of the ductus venosus, indicative of the implication of the AhR in various developmental processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AHR Antibody (NB100-128)(18)

Publications using NB100-128

• Du J, Li S, Gu Y et al. Prevotella histicola inhibits neuroinflammation-induced activation of the Toll-like receptor 4 pathway by promoting the release of interleukin 10 from FoxP3+ Treg cells Research Square 2022-06-21 (IF/IHC, ICC/IF, Human)
• Urzì O, Cafora M, Ganji N et al. Lemon-derived nanovesicles achieve antioxidant and anti-inflammatory effects activating the AhR/Nrf2 signaling pathway iScience 2023-07-01 [PMID: 37426343] (Western Blot, Human)
• Jiang J, Zhu J, Liu Q et al. Role of DNA methylation-related chromatin remodeling in aryl hydrocarbon receptor-dependent regulation of T-2 toxin highly inducible Cytochrome P450 1A4 gene FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-05-01 [PMID: 33788981]
• Liu Q, Wen J, Zhu J et al. Aromatic hydrocarbon receptor regulates chicken cytochrome P450 1A5 transcription: A novel insight into T-2 toxin-induced gene expression and cytotoxicity in LMH cells Biochem. Pharmacol. 2019-07-25 [PMID: 31351871] (WB)
• Carrancá Palomo M, Martín Prieto V, Kirilov P. et Al. Colloidal Dispersions of Gelled Lipid Nanoparticles (GLN): Concept and Potential Applications Gels 2017-09-10 [PMID: 30920529]
• Vogel CF, Khan EM, Leung PS et al. Cross-talk between Aryl Hydrocarbon Receptor and the inflammatory response: a Role for NF-kB. J Biol Chem. 2013-12-03 [PMID: 24302727] (WB, Human, Mouse)
• Vogel CF, Wu D, Goth SR et al. Aryl hydrocarbon receptor signaling regulates NF-kB RelB activation during dendritic-cell differentiation. Immunol Cell Biol. 2013-09-03 [PMID: 23999131] (EMSA, WB, Mouse)
• Vogel CF, Li W, Wu D et al. Interaction of aryl hydrocarbon receptor and NF-kappaB subunit RelB in breast cancer is associated with interleukin-8 overexpression. Arch Biochem Biophys 2011-08-01 [PMID: 21640702] (IF/IHC, Human)
• Gong X, Ye W, Zhou H et al. RanBPM is an acetylcholinesterase-interacting protein that translocates into the nucleus during apoptosis. RanBPM is an acetylcholinesterase-interacting protein that translocates into the nucleus during apoptosis. [PMID: 19902122]
• Dabir P et al. Aryl hydrocarbon receptor is activated by glucose and regulates the thrombospondin-1 gene promoter in endothelial cells. Circ Res;102(12):1558-65. 2008-06-20 [PMID: 18515748] (GS, IF, Human, Rat)
• Fukuda I, Nishiumi S, Yabushita Y et al. A new southwestern chemistry-based ELISA for detection of aryl hydrocarbon receptor transformation: application to the screening of its receptor agonists and antagonists. J Immunol Methods 2004-04-01 [PMID: 15099767] (Human)
• Fisher, M et al. Combined screening of thymocytes using apoptosis-specific cDNA array and promoter analysis yields novel gene targets mediating TCDD-induced toxicity. Toxicol Sci;78(1):116-24. 2004-03-01 [PMID: 14718646] (Chemotaxis, Mouse)
• Minsavage, GD et al. Mutational analysis of the mouse aryl hydrocarbon receptor tyrosine residues necessary for recognition of dioxin response elements. Arch Biochem Biophys;412(1):95-105. 2003-04-01 [PMID: 12646272]
• Pambianchi E, Hagenberg Z, Pecorelli A Et al. Alaskan Bog Blueberry (Vaccinium uliginosum) Extract as an Innovative Topical Approach to Prevent UV-Induced Skin Damage Cosmetics 2021-11-27 (IHC-P, Human)
• Tsuchiya, Y et al. Critical enhancer region to which AhR/ARNT and Sp1 bind in the human CYP1B1 gene. J Biochem;133(5):583-92. 2003-05-01 [PMID: 12801909] (GS, Human)
• Hayashibara, T et al. Possible involvement of aryl hydrocarbon receptor (AhR) in adult T-cell leukemia (ATL) leukemogenesis: constitutive activation of AhR in ATL. Biochem Biophys Res Commun;300(1):128-34. 2003-01-03 [PMID: 12480531]
• Chan, WK et al. Baculovirus expression of the Ah receptor and Ah receptor nuclear translocater. Evidence for additional dioxin responsive element-binding species and factors required for signaling. J Biol Chem;269(42):26464-71. 1994-10-21 [PMID: 7929368]
• Poland, A, Glover, E, Bradfield, CA. Characterization of polyclonal antibodies to the Ah receptor prepared by immunization with a synthetic peptide hapten. Mol Pharmacol;39(1):20-6. 1991-01-01 [PMID: 1846218]

Review for AHR Antibody (NB100-128) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 04/02/2014

Summary

• Application: Western Blot
• Sample Tested: U937 cell nuclear protein
• Species: Human
• Lot: 12802

FAQs for AHR Antibody (NB100-128). (Showing 1 - 1 of 1 FAQs).

1. What is the sodium azide percentage for this product?
   • No preservative 

Bioinformatics

• Gene Symbol: AHR
• Entrez:
  • 196(Human)
  • 11622(Mouse)