AHR Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: AHR Antibody [NB100-128] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated ...read more
Immunohistochemistry-Paraffin: AHR Antibody [NB100-128] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human liver using 20 ug/ml conc. of AHR antibody on a Bond Rx autostainer (Leica Biosystems). The assay ...read more
Gel Super Shift Assays: AHR Antibody [NB100-128] - EMSA validation of AHR antibody on U937 cells nuclear protein. Image submitted by verified customer - see review for protocol details.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ChIP, GS, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

AHR Antibody Summary

Immunogen
N-terminal sequence of Aryl hydrocarbon Receptor purified from C57BL/6J mice. [UniProt# P30561]
Clonality
Polyclonal
Host
Goat
Gene
AHR
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation
  • Gel Super Shift Assays
  • Immunocytochemistry/Immunofluorescence 5-20 ug/ml
  • Immunohistochemistry 20 ug/ml
  • Immunohistochemistry-Paraffin 20 ug/ml
Application Notes
Suggested working dilution for Gel Super Shift Assay (EMSA) is 4ul of antibody to a 15 ul final volume of EMSA sample. The current lot of this antibody did not work in WB to our QC Team's expectations.
Positive Control
AHR Lysate (NBL1-07403)
Reviewed Applications
Read 1 Review rated 5
using
NB100-128 in the following applications:

Publications
Read Publications using
NB100-128 in the following applications:

  • 1 publication
  • 1 publication
  • GS
    2 publications
  • IF
    1 publication
  • IHC
    1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified

Alternate Names for AHR Antibody

  • Ah receptor
  • AHR
  • AH-receptor
  • aryl hydrocarbon receptor
  • BHLHE76
  • bHLHe76aromatic hydrocarbon receptor
  • Class E basic helix-loop-helix protein 76

Background

Aryl hydrocarbon Receptor (AhR) is a ligand-activated transcriptional activator that binds to XRE promoter region of its target genes and has been established to activate phase I/II xenobiotic biotransformation enzyme genes. AhR binds MYBBP1A and its efficient DNA binding requires dimerization with another bHLH proteinsuch as ARNT. Besides interacting with NEDD8 and IVNS1ABP, AhR also interacts with coactivators including SRC-1, RIP140 and NOCA7, and with the corepressor SMRT. AhR localize to cytoplasm and upon binding with ligand followed by interaction with a HSP90, it translocates to the nucleus. AhR has been shown to be induced or repressed by TGFB1 as well as dioxin in a cell-type specific fashion, whereas cAMP, retinoic acid, TPA (12-O-tetradecanoyl phorbol-13 acetate) have been shown to exert repressive effects on AhR. As a member of bHLH-PAS (basic helix-loop-helix Per/ARNT/Sim) family, AhR has been implicated in physiological processes such as circadian cycle, neurogenesis, organs development and regulation of cell-cycle as well as the alternative signaling pathways (independently of exposure to xenobiotics). AhR-null mice exhibit infertility, liver abnormalities and cardiovascular problems such as a defective closure of the ductus venosus, indicative of the implication of the AhR in various developmental processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AHR Antibody (NB100-128)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ChIP, EMSA, GS, IF, IHC, WB.


Filter By Application
ChIP
(1)
EMSA
(1)
GS
(2)
IF
(1)
IHC
(1)
WB
(2)
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Filter By Species
Human
(5)
Mouse
(3)
Rat
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-128 Applications Species
Vogel CF, Kahn EM, Leung PS et al. Cross-talk between Aryl Hydrocarbon Receptor and the inflammatory response: a Role for NF-kB. J Biol Chem 2013 Dec 3 [PMID: 24302727] (WB, Human, Mouse) WB Human, Mouse
Vogel CF, Wu D, Goth SR et al. Aryl hydrocarbon receptor signaling regulates NF-kB RelB activation during dendritic-cell differentiation. Immunol Cell Biol. 2013 Sep 3 [PMID: 23999131] (WB, EMSA, Mouse) WB, EMSA Mouse
Vogel CF, Li W, Wu D et al. Interaction of aryl hydrocarbon receptor and NF-?B subunit RelB in breast cancer is associated with interleukin-8 overexpression Arch Biochem Biophys 2011 Aug [PMID: 21640702] (IHC, Human) IHC Human
Dabir P et al. Aryl hydrocarbon receptor is activated by glucose and regulates the thrombospondin-1 gene promoter in endothelial cells. Circ Res;102(12):1558-65. 2008 Jun 20. [PMID: 18515748] (GS, IF, Human, Rat) GS, IF Human, Rat
Fukuda I, Nishiumi S, Yabushita Y et al. A new southwestern chemistry-based ELISA for detection of aryl hydrocarbon receptor transformation: application to the screening of its receptor agonists and antagonists. J Immunol Methods 2004 Apr [PMID: 15099767] (Human) Human
Fisher, M et al. Combined screening of thymocytes using apoptosis-specific cDNA array and promoter analysis yields novel gene targets mediating TCDD-induced toxicity. Toxicol Sci;78(1):116-24. 2004 Mar. [PMID: 14718646] (ChIP, Mouse) ChIP Mouse
Tsuchiya, Y et al. Critical enhancer region to which AhR/ARNT and Sp1 bind in the human CYP1B1 gene. J Biochem;133(5):583-92. 2003 May. [PMID: 12801909] (GS, Human) GS Human
Minsavage, GD et al. Mutational analysis of the mouse aryl hydrocarbon receptor tyrosine residues necessary for recognition of dioxin response elements. Arch Biochem Biophys;412(1):95-105. 2003 Apr 1. [PMID: 12646272]
Hayashibara, T et al. Possible involvement of aryl hydrocarbon receptor (AhR) in adult T-cell leukemia (ATL) leukemogenesis: constitutive activation of AhR in ATL. Biochem Biophys Res Commun;300(1):128-34. 2003 Jan 3. [PMID: 12480531]
Chan, WK et al. Baculovirus expression of the Ah receptor and Ah receptor nuclear translocater. Evidence for additional dioxin responsive element-binding species and factors required for signaling. J Biol Chem;269(42):26464-71. 1994 Oct 21. [PMID: 7929368]
Show All 11 Publications.

Review for AHR Antibody (NB100-128) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-128:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot AHR NB100-128
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reviewed by:
Anonymous
WB Human 04/02/2014
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Summary

ApplicationWestern Blot
Sample TestedU937 cell nuclear protein
SpeciesHuman
Lot12802
CommentsDNA-protein binding reactions were carried out in a total volume of 15 μl containing 20 μg nuclear protein, 60,000 cpm of DNA oligonucleotide, 25 mM Tris buffer (pH 7.5), 50 mM NaCl, 1 mM EDTA, 0.5 mM dithiothreitol, 5% glycerol, and 1 μg poly (dI-dC). The samples were incubated at room temperature for 20 min. Supershift analysis were performed by adding 4 μl of polyclonal AhR (Novus Biologicals, Littleton, CO) antibodies to the reaction mixtures. Competition experiments were performed in the presence of a 100-fold molar excess of unlabeled DNA fragments. Protein-DNA complexes were resolved on a 4% nondenaturating polyacrylamide gel and visualized by exposure of the dehydrated gels to X-ray films.

Blocking

Blocking Details5 % milk powder

Primary Anitbody

Dilution Ratio1:100

Secondary Antibody

Secondary Descriptionmous anti-goat
Secondary Manufacturer Cat#NB100
Secondary Concentration1000 ul crude extract

Details

Detection NotesEMSA

Comments

CommentsDNA-protein binding reactions were carried out in a total volume of 15 μl containing 20 μg nuclear protein, 60,000 cpm of DNA oligonucleotide, 25 mM Tris buffer (pH 7.5), 50 mM NaCl, 1 mM EDTA, 0.5 mM dithiothreitol, 5% glycerol, and 1 μg poly (dI-dC). The samples were incubated at room temperature for 20 min. Supershift analysis were performed by adding 4 μl of polyclonal AhR (Novus Biologicals, Littleton, CO) antibodies to the reaction mixtures. Competition experiments were performed in the presence of a 100-fold molar excess of unlabeled DNA fragments. Protein-DNA complexes were resolved on a 4% nondenaturating polyacrylamide gel and visualized by exposure of the dehydrated gels to X-ray films.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for AHR Antibody (NB100-128). (Showing 1 - 1 of 1 FAQs).

  1. What is the sodium azide percentage for this product?
    • No preservative 

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional AHR Products

Bioinformatics Tool for AHR Antibody (NB100-128)

Discover related pathways, diseases and genes to AHR Antibody (NB100-128). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for AHR Antibody (NB100-128)

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Pathways for AHR Antibody (NB100-128)

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PTMs for AHR Antibody (NB100-128)

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Research Areas for AHR Antibody (NB100-128)

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Blogs on AHR.

AHR - A transcription factor regulating immunity and tumorigenesis
The aryl hydrocarbon receptor (AHR) is a ligand activated transcription factor that controls the expression of a diverse set of genes. In the absence of ligand, AHR is retained in the cytoplasm. Upon ligand binding AHR translocates to the nucleus w...  Read full blog post.

Aryl Hydrocarbon Signaling: AIP, AhR, ARNT, BMAL1 and more...
AH receptor-interacting protein (AIP) is a 37 kD immunophilin-like factor found in a variety of tissues with expression levels ranging from high (spleen, thymus, pituitary heart, placenta and skeletal muscle) to low (liver, kidney and lung). It mediat...  Read full blog post.

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Recent Reviews

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1
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Anonymous
04/02/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol AHR
Entrez