Prokineticin R2/PROKR2 Antibody

Western Blot: Prokineticin R2/PROKR2 Antibody [NBP1-45218] - 0.1ug/ml staining of Human Cer(A), Rat Brain (B) and Rat Testis (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Prokineticin R2/PROKR2 Antibody [NBP1-45218] - 3.75ug/ml staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: Prokineticin R2/PROKR2 Antibody [NBP1-45218] - (1ug/ml) staining of Rat Stomach lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Prokineticin R2/PROKR2 Antibody Summary

• Immunogen: Peptide with sequence C-NGNTSFTPNFN corresponding to internal region according to NP_658986.1.
• Epitope: internal region
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PROKR2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3-1 ug/ml
• Application Notes: Western blot: Approx 45kDa band observed in Human Brain (Cerebellum), Testis and Thyroid Gland and in Rat Brain, Stomach and Testis lysates (calculated MW of 44.0kDa according to Human NP_658986.1 and 47.9kDa according to Rat NP_620434.1). Primary incubation was 1 hour
  IHC: Paraffin embedded Human Brain (Cortex).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Prokineticin R2/PROKR2 Antibody

• dJ680N4.3
• G protein-coupled receptor 73-like 1
• GPR73bG-protein coupled receptor 73-like 1
• GPR73L1
• GPR73L1Kallmann syndrome 3 (autosomal dominant)
• GPRg2
• GPRg2KAL3
• KAL3
• PKR2
• PKR2PK-R2
• Prokineticin R2
• prokineticin receptor 2
• ProkineticinR2
• PROKR2

Background

Corticotropin Releasing Factor Receptor 2 (CRHR2) expression has been reported in various regions of the brain, as well as in placenta, umbilical vein, heart, epididymis, gastrointestinal tract, adrenal, and skeletal muscle. It shows high-affinity CRF binding and also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. CRCH2 alpha is the dominant isoform and is expressed widely. CRCH2 beta is expressed in the hippocampus, septum, amygdala, heart, and skeletal muscle. CRHR2 gamma is brain-specific. ESTs have been isolated from brain libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Prokineticin R2/PROKR2 Antibody (NBP1-45218)(1)

Publication using NBP1-45218

• Monnier C, Dode C, Fabre L et al. PROKR2 missense mutations associated with Kallmann syndrome impair receptor signalling activity. Hum Mol Genet 2009-01-01 [PMID: 18826963]

Bioinformatics

• Gene Symbol: PROKR2
• Entrez:
  • 128674(Human)
  • 192649(Rat)
• Uniprot:
  • NP_658986.1(Human)