LMAN1 Antibody (OTI1A8)

Images

 
Western Blot: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Analysis of extracts (35ug) from 9 different cell lines by using anti-LMAN1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LMAN1.
Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Staining of paraffin-embedded Human prostate tissue using anti-LMAN1 mouse monoclonal antibody.
Flow Cytometry: LMAN1 Antibody (OTI1A8) [NBP2-03381] - HEK293T cells transfected with either RC207088 overexpress plasmid(Red) or empty vector control plasmid(Blue) wereimmunostained by anti-LMAN1 antibodyand then ...read more
Western Blot: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Staurosporine-induced cleavage of SMSr is sensitive to caspase-6 inhibitors. HeLa cells stably transduced with EV, SMSr-V5, and SMSrCR-V5 were treated with 1 ug/ml ...read more
Western Blot: LMAN1 Antibody (OTI1A8) [NBP2-03381] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMAN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-LMAN1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LMAN1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Staining of paraffin-embedded Human Kidney tissue using anti-LMAN1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Staining of paraffin-embedded Human liver tissue using anti-LMAN1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Staining of paraffin-embedded Human pancreas tissue using anti-LMAN1 mouse monoclonal antibody.
Flow Cytometry: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Analysis of Jurkat cells, using anti-LMAN1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI1A8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

LMAN1 Antibody (OTI1A8) Summary

Immunogen
Full length human recombinant protein of human LMAN1 (NP_005561) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LMAN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:200-500
Theoretical MW
54.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-03381 in the following applications:

Reactivity Notes

Monkey reactivity reported in scientific literature (PMID: 24891604). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LMAN1 Antibody (OTI1A8)

  • ERGIC-53
  • ERGIC53ER-Golgi intermediate compartment 53 kDa protein
  • F5F8Dendoplasmic reticulum-golgi intermediate compartment protein 53
  • FMFD1
  • Gp58
  • intracellular mannose specific lectin
  • Intracellular mannose-specific lectin MR60
  • Lectin mannose-binding 1
  • lectin, mannose-binding, 1
  • MCFD1coagulation factor V-factor VIII combined deficiency
  • MR60
  • protein ERGIC-53

Background

The protein encoded by this gene is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LMAN1 Antibody (NBP2-03381)(2)

We have publications tested in 2 confirmed species: Human, Monkey.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(1)
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(1)
Monkey
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol LMAN1
Entrez