PKR Antibody

Images

 
Western Blot: PKR Antibody [NBP1-77266] - A431 whole cell lysate with PKR antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: PKR Antibody [NBP1-77266] - Mouse lung tissue with PKR antibody at 20 ug/ml.
Immunohistochemistry: PKR Antibody [NBP1-77266] - Staining of mouse lung tissue with antibody at 5 ug/mL.
Immunohistochemistry-Paraffin: PKR Antibody [NBP1-77266] - Rat lung tissue with PKR antibody at 2.5 ug/ml.
Immunofluorescence: PKR Antibody [NBP1-77266] - Immunofluorescence of PKR in Rat Lung tissue with PKR antibody at 20 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PKR Antibody Summary

Immunogen
Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human PKR. The immunogen is located within the last 50 amino acids of PKR.
Specificity
At least two different isoforms of PKR are known to exist; this antibody will detect both isoforms.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF2AK2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunofluorescence 20 ug/ml
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
PKR Peptide (NBP1-77266PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-77266 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PKR Antibody

  • double stranded RNA activated protein kinase
  • EC 2.7.11.1
  • eIF-2A protein kinase 2
  • EIF2AK1
  • EIF2AK2
  • eukaryotic translation initiation factor 2-alpha kinase 2P1/eIF-2A protein kinase
  • interferon-induced, double-stranded RNA-activated protein kinase
  • interferon-inducible double stranded RNA dependent
  • interferon-inducible elF2alpha kinase
  • Interferon-inducible RNA-dependent protein kinase
  • P68 Kinase
  • PKR
  • PKRp68 kinase
  • PPP1R83
  • PRKR
  • PRKRMGC126524
  • Protein Kinase R
  • Protein kinase RNA-activated

Background

The interferon-inducible, double-stranded RNA (dsRNA)-dependent protein kinase PKR is a member of the eukaryotic initiation factor-2 alpha (eIF2-alpha) kinase family, possessing serine-threonine kinase activity and two dsRNA-binding motifs that acts as part of the innate immune system. Upon binding dsRNA, PKR undergoes a conformational change leading to its activation and its phosphorylation of the translation factor eIF2, resulting in a general shutdown of protein synthesis and induction of apoptosis through upregulation of caspase-8 and capsase-9 activity in order to prevent the production of more viruses. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PKR Antibody (NBP1-77266) (0)

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Review for PKR Antibody (NBP1-77266) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-77266:
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Images Ratings Applications Species Date Details
Western Blot PKR NBP1-77266
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reviewed by:
Liyong Zhang
WB Mouse 06/27/2014
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Summary

ApplicationWestern Blot
SpeciesMouse
FileView PDF

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PKR Antibody (NBP1-77266) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for PKR Antibody (NBP1-77266)

Discover related pathways, diseases and genes to PKR Antibody (NBP1-77266). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for PKR Antibody (NBP1-77266)

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Blogs on PKR.

eIF2alpha - a regulator of global translation in response to cellular stress
Eukaryotic initiation factor 2 (eIF2) regulates global protein translation by binding to Met-tRNA and the 40S ribosome to form the pre-initiation complex. eIF2 is a heterotrimer consisting of alpha, beta, and gamma subunits. The 36kDA eIF2a subuni...  Read full blog post.

PKR - Mediating cellular stress responses through multiple signaling pathways
Protein kinase R (PKR) is an intracellular stress-sensing protein that is able to detect and respond to viral infections. While PKR is able to sense and respond to a variety of signals, dsRNA is a well-characterized ligand. dsRNA produced during v...  Read full blog post.

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Recent Reviews

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5
1
4
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2
0
1
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Liyong Zhang
06/27/2014
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol EIF2AK2
Entrez
Uniprot