PKR Antibody


Western Blot: PKR Antibody [NBP1-77266] - A431 whole cell lysate with PKR antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: PKR Antibody [NBP1-77266] - Mouse lung tissue with PKR antibody at 20 ug/ml.
Immunohistochemistry: PKR Antibody [NBP1-77266] - Staining of mouse lung tissue with antibody at 5 ug/mL.
Immunohistochemistry-Paraffin: PKR Antibody [NBP1-77266] - Rat lung tissue with PKR antibody at 2.5 ug/ml.
Immunofluorescence: PKR Antibody [NBP1-77266] - Immunofluorescence of PKR in Rat Lung tissue with PKR antibody at 20 ug/mL.

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IF
1 mg/ml

Order Details

PKR Antibody Summary

Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human PKR.
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1 mg/ml
Immunogen affinity purified


  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunofluorescence 20 ug/ml
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
PKR Peptide (NBP1-77266PEP)
Reviewed Applications
Read 1 Review rated 5
NBP1-77266 in the following applications:

Alternate Names for PKR Antibody

  • double stranded RNA activated protein kinase
  • EC
  • eIF-2A protein kinase 2
  • EIF2AK1
  • EIF2AK2
  • eukaryotic translation initiation factor 2-alpha kinase 2P1/eIF-2A protein kinase
  • interferon-induced, double-stranded RNA-activated protein kinase
  • interferon-inducible double stranded RNA dependent
  • interferon-inducible elF2alpha kinase
  • Interferon-inducible RNA-dependent protein kinase
  • P68 Kinase
  • PKRp68 kinase
  • PPP1R83
  • PRKR
  • PRKRMGC126524
  • Protein Kinase R
  • Protein kinase RNA-activated


The interferon-inducible, double-stranded RNA (dsRNA)-dependent protein kinase PKR is a member of the eukaryotic initiation factor-2 alpha (eIF2-alpha) kinase family, possessing serine-threonine kinase activity and two dsRNA-binding motifs that acts as part of the innate immune system. Upon binding dsRNA, PKR undergoes a conformational change leading to its activation and its phosphorylation of the translation factor eIF2, resulting in a general shutdown of protein synthesis and induction of apoptosis through upregulation of caspase-8 and capsase-9 activity in order to prevent the production of more viruses. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PKR Antibody (NBP1-77266) (0)

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Reviews for PKR Antibody (NBP1-77266) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-77266:
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Images Ratings Applications Species Date Details
Western Blot PKR NBP1-77266
reviewed by:
WB Mouse 06/27/2014


ApplicationWestern Blot
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PKR Antibody (NBP1-77266) (0)

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Secondary Antibodies


Isotype Controls

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Research Areas for PKR Antibody (NBP1-77266)

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Blogs on PKR.

eIF2alpha - a regulator of global translation in response to cellular stress
Eukaryotic initiation factor 2 (eIF2) regulates global protein translation by binding to Met-tRNA and the 40S ribosome to form the pre-initiation complex. eIF2 is a heterotrimer consisting of alpha, beta, and gamma subunits. The 36kDA eIF2a subuni...  Read full blog post.

PKR - Mediating cellular stress responses through multiple signaling pathways
Protein kinase R (PKR) is an intracellular stress-sensing protein that is able to detect and respond to viral infections. While PKR is able to sense and respond to a variety of signals, dsRNA is a well-characterized ligand. dsRNA produced during v...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol EIF2AK2

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