ZAK Antibody (3G5)

Western Blot: ZAK Antibody (3G5) [H00051776-M03] - ZAK monoclonal antibody (M03), clone 3G5. Western Blot analysis of ZAK expression in human stomach.

Immunocytochemistry/Immunofluorescence: ZAK Antibody (3G5) [H00051776-M03] - Analysis of monoclonal antibody to ZAK on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: ZAK Antibody (3G5) [H00051776-M03] - Analysis of monoclonal antibody to ZAK on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3G5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ZAK Antibody (3G5) Summary

• Immunogen: ZAK (AAH01401, 1 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHY
• Specificity: ZAK - sterile alpha motif and leucine zipper containing kinase AZK
• Isotype: IgG3 Lambda
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZAK
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Has also been used for immunohistochemistry (paraffin).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ZAK Antibody (3G5)

• AZK
• EC 2.7.11
• EC 2.7.11.25
• HCCS-4
• Human cervical cancer suppressor gene 4 protein
• Leucine zipper- and sterile alpha motif-containing kinase
• mitogen-activated protein kinase kinase kinase MLT
• mixed lineage kinase 7
• mixed lineage kinase with a leucine zipper and a sterile alpha motif
• mixed lineage kinase-related kinase MRK-beta
• Mixed lineage kinase-related kinase
• MLK7
• mlklak
• MLK-like mitogen-activated protein triple kinase
• MLK-related kinase
• MLT
• MLTK
• MRKcervical cancer suppressor gene 4 protein
• pk
• protein kinase
• sterile alpha motif and leucine zipper containing kinase AZK
• Sterile alpha motif- and leucine zipper-containing kinase AZK

Background

This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZAK Antibody (H00051776-M03)(3)

Publications using H00051776-M03

• Nyati S, Chator A, Schinske K et al. A Requirement for ZAK Kinase Activity in Canonical TGF-B Signaling. Transl Oncol 2016-10-23 [PMID: 27783979]
• Perez Lopez I, Cariolato L, Maric D et al. A-Kinase Anchoring Protein Lbc Coordinates a p38 Activating Signaling Complex Controlling Compensatory Cardiac Hypertrophy. Mol Cell Biol. 2013-05-28 [PMID: 23716597]
• Cariolato L, Cavin S, Diviani D. AKAP-LBC anchors a PKN-based signaling complex involved in alpha1-adrenergic receptor-induced p38 activation. J Biol Chem. 2011-01-11 [PMID: 21224381]

Bioinformatics

• Gene Symbol: ZAK
• Entrez:
  • 51776(Human)
• OMIM:
  • 609479(Human)
• Uniprot:
  • AAH01401(Human)