Prokineticin 2 Antibody

Immunocytochemistry/Immunofluorescence: Prokineticin 2 Antibody [NBP2-43853] - Localization of Bv8/PK2 in fasciculata cells.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

Prokineticin 2 Antibody Summary

• Immunogen: Synthetic peptide derived from Cter region of human PK2
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PROK2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:200-1:1000
• Application Notes: Use in IHC reported in scientific literature (PMID: 30046389).

Packaging, Storage & Formulations

• Storage: Lyophilized antibody can be kept at 4C for up to 3 months and should be kept at -20C for long-term storage. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20C) or for long-term storage (-80C). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
• Buffer: 0.1M Tris , 0.1M Glycine and 2% Sucrose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Reconstitute in distilled water to final concentration of 1mg/ml.

Alternate Names for Prokineticin 2 Antibody

• BV8
• BV8Protein Bv8 homolog
• KAL4
• MIT1
• PK2prokineticin-2
• PROK2
• Prokineticin 2
• Protein Bv8 homolog

Background

Prokineticin 2 is known to regulate many different biological functions, including neurogenesis, smooth muscle contractility, angiogenesis and circadian rhythm. In serving the latter role, prokineticin 2 functions as an output molecule from the suprachiasmatic nucleus (SCN) of the hypothalamus, that transmits behavioral rhythms, but may also function locally within the SCN to synchronize output. Prokineticin 2 expression is induced by CLOCK and BMAL1 heterodimers and light, and is inhibited by period genes (PER1, PER2 and PER3) and cryptochrome genes (CRY1 and CRY2). Expression is reported in the SCN and among a few other discrete brain areas, including the islands of Calleja, media l preoptic area of the hypothalamus and the shell of the nucleus accumbens as well as in the testis, prostate and, at lower levels, in the small intestine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Prokineticin 2 Antibody (NBP2-43853)(1)

Publication using NBP2-43853

• Yoshida Y, Goi T, Kurebayashi H et al. Prokineticin 2 expression as a novel prognostic biomarker for human colorectal cancer. Oncotarget 2018-07-10 [PMID: 30046389] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: PROK2