| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-48308SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-14833SS | |||
HAF016). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A549 human lung carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, BaF3 mouse pro-B cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat PKM1/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7244) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7244-SP | |||
NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="PKM2 was detected in immersion fixed WI-38 human lung fibroblast cell line using Mouse Anti-Human/Rat PKM1/2 Monoclonal Antibody (Catalog # MAB7244) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7244-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21688-50ul | |||
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody - BSA Free [NBP1-48308] - Analysis of PKM2 in frozen sections of rat glioblastoma tissue. Image courtsey of anonymous customer review.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-48308-img0007.jpg)
![Immunohistochemistry: PKM2 Antibody - BSA Free [NBP1-48308] - Staining of PKM2 in mouse liver tissue.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Immunohistochemistry-NBP1-48308-img0009.jpg)
![Flow Cytometry: PKM2 Antibody - BSA Free [NBP1-48308] - An intracellular stain was performed on Neuro2a cells with PKM2 Antibody NBP1-48308AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Flow-Cytometry-NBP1-48308-img0019.jpg)
![Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Analysis of HeLa lysates using NBP1-48308. Image courtesy of Gregg Semenza, (PMID: 21620138).](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Western-Blot-NBP1-48308-img0010.jpg)
![Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Analysis of PKM2 in MCF7 whole cell lysates.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Western-Blot-NBP1-48308-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody - BSA Free [NBP1-48308] - Analysis of PKM2 in HeLa cells.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-48308-img0008.jpg)
![Flow Cytometry: PKM2 Antibody - BSA Free [NBP1-48308] - An intracellular stain was performed on HeLa cells with PKM2 Antibody NBP1-48308AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Flow-Cytometry-NBP1-48308-img0014.jpg)
![Simple Western: PKM2 Antibody - BSA Free [NBP1-48308] - Lane view shows a specific band for PKM2 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.](http://images.novusbio.com/fullsize/PKM2-Antibody---BSA-Free-Simple-Western-NBP1-48308-img0013.jpg)
![Immunohistochemistry-Paraffin: PKM1 Antibody [NBP2-14833] - PKM1 antibody was tested in human breast cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/PKM1-Antibody-Immunohistochemistry-Paraffin-NBP2-14833-img0002.jpg)
![Western Blot: PKM1 Antibody [NBP2-14833] - PKM1 antibody was tested in 1. human skeletal muscle 2. mouse skeletal muscle 3. human brain 4. mouse brain and 5. human heart cell lysate.](http://images.novusbio.com/fullsize/PKM1-Antibody-Western-Blot-NBP2-14833-img0003.jpg)
![Western Blot: PKM1 Antibody [NBP2-14833] - Detection of PKM1 on HEK293T and U-87 cells. Lane 1: HEK293T cells (major isoform is PKM2, human). Lane 2: HEK293T cells with plasmid overexpressing mouse PKM1. Lane 3: HEK293T cells with plasmid overexpressing mouse PKM2. Lane 4: U-87 cells with control shRNA (major isoform is PKM2, human). Lane 5: U-87 cells with PTB/A1/A2 shRNAs (major isoform is PKM1, human). Photo courtesy of product review by verified customer.](http://images.novusbio.com/fullsize/PKM1-Antibody-Western-Blot-NBP2-14833-img0004.jpg)
![Simple Western: PKM1 Antibody [NBP2-14833] - Simple Western lane view shows a specific band for PKM1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PKM1-Antibody-Simple-Western-NBP2-14833-img0005.jpg)
![Immunohistochemistry-Paraffin: PKM1 Antibody - BSA Free [NBP2-14833] - Expression of PKM1 & PKM2 in clinical colorectal cancer samples.(a) The protein expression of PKM1 & PKM2 in clinical specimens of cancer tumor (T) & the adjacent normal tissues (N) is shown. PKM1 & PKM2 were detected by Western blotting in under the same experimental conditions at the same time. The full-length blots are presented in Supplementary Figure S3b. (b–d) Immunohistochemical staining of normal colon tissue adjacent to tumor tissue of case 10. Results of H&E staining (b), staining with anti-PKM1 antibody (c), & staining with anti-PKM2 (d) are shown. The boxed regions in “c” & “d” are enlarged in the images below. (e–h) Immunohistochemical staining of clinical colorectal cancer tissue specimen of representative case 3. H&E-stained section with normal tissue (upper right corner) neighboring the tumor area in the section is shown (e), along with the same section stained with anti-PKM2 antibody (f). Enlarged views of boxed areas in “f” show normal colorectal crypt in mucosa (g) & tumor area (h) stained with anti-PKM2 antibody. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14833_rabbit-polyclonal-pkm1-antibody-31020241612233.jpg)
![Western Blot: PKM1 Antibody - BSA Free [NBP2-14833] - (a) Luciferase activities after co-transfection of DLD-1 cells w/ control or miR-124 (wild-type or mutant-type) pMIR vectors having predictive miR-124 binding site in 3′UTR of PTB1. Upper panel region of 3′-UTR of human PTB1 mRNA complementary to mature miR-124. Box indicates predicted binding sites for miR-124. (b) Same as “a” except miR-133b used. (c) Expression of PTB1, PKM1, & PKM2 proteins at 72 h after transfection of DLD-1, NB9 or IMR-32 cells w/ miR-124 (10, 20 or 40 nM). (d) Expression of PTB1, PKM1, & PKM2 proteins at 72 h after transfection of DLD-1, RD or KYM-1 cells w/ miR-133b (10, 20 nM). (e) Expression of PTB1, PKM1, & PKM2 proteins at 72 h after transfection of DLD-1, NB-9 or RD cells w/ siR-PTB1 (2, 5 nM). (f) Effect of combined treatment of DLD-1 cells w/ antagomiR-124 & miR-124 or antagomiR-133b & miR-133b. DLD-1 cells transfected w/ non-specific control, miR-124/miR-133b (10 nM), miR-124/miR-133b (10 nM) + antagomiR-124/antagomiR-133b (5 nM) or miR-124/miR-133b (10 nM) + antagomiR-124/antagomiR-133b (10 nM). Expression level of PTB1 assessed at 48 h after transfection. The full-length blots are presented in Supplementary Figure S3a. (g) IF of PKM1 (upper panels) & PKM2 (lower panels) at 48 h after transfection of DLD-1 cells w/ miR-124 (20 nM) or miR-133b (20 nM). Left panels, treatment w/ control miRNA; middle panels, treatment w/ miR-124; right panels, treatment w/ miR-133b. PKM1 or PKM2 is stained red, & nuclei are stained blue. (h) Lactate production measured at 48 h after transfection of DLD-1 cells w/ miR-124 (20 nM), miR-133b (20 nM) or siR-PTB1 (5 nM). Results are presented as mean± SD (* P < 0.05; ** P < 0.01; *** P < 0.001; N.S., not statistically significant). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14833_rabbit-polyclonal-pkm1-antibody-31020241612196.jpg)
![Immunocytochemistry/ Immunofluorescence: PKM1 Antibody - BSA Free [NBP2-14833] - (a) Luciferase activities after co-transfection of DLD-1 cells w/ control or miR-124 (wild-type or mutant-type) pMIR vectors having predictive miR-124 binding site in 3′UTR of PTB1. Upper panel region of 3′-UTR of human PTB1 mRNA complementary to mature miR-124. Box indicates predicted binding sites for miR-124. (b) Same as “a” except miR-133b used. (c) Expression of PTB1, PKM1, & PKM2 proteins at 72 h after transfection of DLD-1, NB9 or IMR-32 cells w/ miR-124 (10, 20 or 40 nM). (d) Expression of PTB1, PKM1, & PKM2 proteins at 72 h after transfection of DLD-1, RD or KYM-1 cells w/ miR-133b (10, 20 nM). (e) Expression of PTB1, PKM1, & PKM2 proteins at 72 h after transfection of DLD-1, NB-9 or RD cells w/ siR-PTB1 (2, 5 nM). (f) Effect of combined treatment of DLD-1 cells w/ antagomiR-124 & miR-124 or antagomiR-133b & miR-133b. DLD-1 cells transfected w/ non-specific control, miR-124/miR-133b (10 nM), miR-124/miR-133b (10 nM) + antagomiR-124/antagomiR-133b (5 nM) or miR-124/miR-133b (10 nM) + antagomiR-124/antagomiR-133b (10 nM). Expression level of PTB1 assessed at 48 h after transfection. The full-length blots are presented in Supplementary Figure S3a. (g) IF of PKM1 (upper panels) & PKM2 (lower panels) at 48 h after transfection of DLD-1 cells w/ miR-124 (20 nM) or miR-133b (20 nM). Left panels, treatment w/ control miRNA; middle panels, treatment w/ miR-124; right panels, treatment w/ miR-133b. PKM1 or PKM2 is stained red, & nuclei are stained blue. (h) Lactate production measured at 48 h after transfection of DLD-1 cells w/ miR-124 (20 nM), miR-133b (20 nM) or siR-PTB1 (5 nM). Results are presented as mean± SD (* P < 0.05; ** P < 0.01; *** P < 0.001; N.S., not statistically significant). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14833_rabbit-polyclonal-pkm1-antibody-31020241613613.jpg)
![Western Blot: PKM1 Antibody - BSA Free [NBP2-14833] - (a) Luciferase activities after co-transfection of DLD-1 cells w/ control or miR-124 (wild-type or mutant-type) pMIR vectors having predictive miR-124 binding site in 3′UTR of PTB1. Upper panel region of 3′-UTR of human PTB1 mRNA complementary to mature miR-124. Box indicates predicted binding sites for miR-124. (b) Same as “a” except miR-133b used. (c) Expression of PTB1, PKM1, & PKM2 proteins at 72 h after transfection of DLD-1, NB9 or IMR-32 cells w/ miR-124 (10, 20 or 40 nM). (d) Expression of PTB1, PKM1, & PKM2 proteins at 72 h after transfection of DLD-1, RD or KYM-1 cells w/ miR-133b (10, 20 nM). (e) Expression of PTB1, PKM1, & PKM2 proteins at 72 h after transfection of DLD-1, NB-9 or RD cells w/ siR-PTB1 (2, 5 nM). (f) Effect of combined treatment of DLD-1 cells w/ antagomiR-124 & miR-124 or antagomiR-133b & miR-133b. DLD-1 cells transfected w/ non-specific control, miR-124/miR-133b (10 nM), miR-124/miR-133b (10 nM) + antagomiR-124/antagomiR-133b (5 nM) or miR-124/miR-133b (10 nM) + antagomiR-124/antagomiR-133b (10 nM). Expression level of PTB1 assessed at 48 h after transfection. The full-length blots are presented in Supplementary Figure S3a. (g) IF of PKM1 (upper panels) & PKM2 (lower panels) at 48 h after transfection of DLD-1 cells w/ miR-124 (20 nM) or miR-133b (20 nM). Left panels, treatment w/ control miRNA; middle panels, treatment w/ miR-124; right panels, treatment w/ miR-133b. PKM1 or PKM2 is stained red, & nuclei are stained blue. (h) Lactate production measured at 48 h after transfection of DLD-1 cells w/ miR-124 (20 nM), miR-133b (20 nM) or siR-PTB1 (5 nM). Results are presented as mean± SD (* P < 0.05; ** P < 0.01; *** P < 0.001; N.S., not statistically significant). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14833_rabbit-polyclonal-pkm1-antibody-310202416113524.jpg)
![Western Blot: PKM1 Antibody - BSA Free [NBP2-14833] - Expression of PKM1 & PKM2 in clinical colorectal cancer samples.(a) The protein expression of PKM1 & PKM2 in clinical specimens of cancer tumor (T) & the adjacent normal tissues (N) is shown. PKM1 & PKM2 were detected by Western blotting in under the same experimental conditions at the same time. The full-length blots are presented in Supplementary Figure S3b. (b–d) Immunohistochemical staining of normal colon tissue adjacent to tumor tissue of case 10. Results of H&E staining (b), staining with anti-PKM1 antibody (c), & staining with anti-PKM2 (d) are shown. The boxed regions in “c” & “d” are enlarged in the images below. (e–h) Immunohistochemical staining of clinical colorectal cancer tissue specimen of representative case 3. H&E-stained section with normal tissue (upper right corner) neighboring the tumor area in the section is shown (e), along with the same section stained with anti-PKM2 antibody (f). Enlarged views of boxed areas in “f” show normal colorectal crypt in mucosa (g) & tumor area (h) stained with anti-PKM2 antibody. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14833_rabbit-polyclonal-pkm1-antibody-3102024161364.jpg)
![Western Blot: PKM1 Antibody - BSA Free [NBP2-14833] - Characterization of NOX4 & PKM2 in human RCC tumors & adjacent tissue. a Mitochondrial fractions were prepared from human tumors (T) or uninvolved adjacent tissue (N). NOX4 expression was examined by western blot analysis. Prohibitin was probed as a mitochondrial marker & loading control. b Quantitation of NOX4 distribution in the mitochondrial fraction from a. The results are expressed as the means using one-way ANOVA with Tukey’s post hoc test where ± S.E.M. *p < 0.05 compared to normal (N). c Mitochondria fractions were prepared from RCC tumors & NADPH-dependent superoxide generation was examined in the presence (+) or absence (−) of ATP. The results are from eight tumors & are expressed as the means using one-way ANOVA with Tukey’s post hoc test where ±S.E.M. **p < 0.01 is compared to without (−) ATP. d PKM2 & PKM1 expression was examined by western blot analysis in lysates prepared from human tumors (T) or uninvolved adjacent tissue (N) from the same patient. Actin as loading control Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29051480), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14833_rabbit-polyclonal-pkm1-antibody-310202416121963.jpg)
NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for 
NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for 
CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to Sertoli cells in testis. View our protocol for 
CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to Sertoli cells in testis. View our protocol for 
HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
HAF016). A specific band was detected for PKM2 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (
HAF016). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />
CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cell cytoplasm. View our protocol for 
NL007) and counterstained with DAPI (blue). Specific staining was localized to epithelial cell cytoplasm. View our protocol for 
HAF018). A specific band was detected for PKM2 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (
NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for 
HAF018). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />
![Western Blot: PKM2 Antibody [NBP1-82487] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/PKM2-Antibody-Western-Blot-NBP1-82487-img0012.jpg "Western Blot: PKM2 Antibody [NBP1-82487] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody [NBP1-82487] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PKM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82487-img0009.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PKM2 Antibody [NBP1-82487] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PKM antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/PKM2-Antibody-Western-Blot-NBP1-82487-img0011.jpg)
![Western Blot: PKM2 Antibody (7B2) [NBP3-15230] - Positive WB detected in: SH-SY5Y whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse brain tissue. All lanes: PKM2 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58, 59, 57 KDa. Observed band size: 58 KDa.](http://images.novusbio.com/fullsize/PKM2-Antibody-7B2-Western-Blot-NBP3-15230-img0005.jpg "Western Blot: PKM2 Antibody (7B2) [NBP3-15230] - Positive WB detected in: SH-SY5Y whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse brain tissue. All lanes: PKM2 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58, 59, 57 KDa. Observed band size: 58 KDa.")
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody (7B2) [NBP3-15230] - Immunofluorescence staining of Hela Cells with at PKM2 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/PKM2-Antibody-7B2-Immunocytochemistry-Immunofluorescence-NBP3-15230-img0002.jpg)
![Immunohistochemistry: PKM2 Antibody (7B2) [NBP3-15230] - IHC image of PKM2 antibody diluted at 1:100 and staining in paraffin-embedded human lung cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/PKM2-Antibody-7B2-Immunohistochemistry-NBP3-15230-img0004.jpg)
![Flow Cytometry: PKM2 Antibody (7B2) [NBP3-15230] - Overlay histogram showing HepG2 cells stained with PKM2 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/PKM2-Antibody-7B2-Flow-Cytometry-NBP3-15230-img0001.jpg)
![Immunoprecipitation: PKM2 Antibody (7B2) [NBP3-15230] - Immunoprecipitating PKM2 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of PKM2 antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: PKM2 antibody (2ug)+ Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).](http://images.novusbio.com/fullsize/PKM2-Antibody-7B2-Immunoprecipitation-NBP3-15230-img0003.jpg)
![Western Blot: PKM2 Antibody [NBP2-19852] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody.](http://images.novusbio.com/fullsize/PKM2-Antibody-Western-Blot-NBP2-19852-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PKM2 Antibody [NBP2-19852] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: PKM2 Antibody [NBP2-19852] - antibody detects PKM2 protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Pyruvate Kinase (muscle) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/PKM2-Antibody-Immunohistochemistry-Paraffin-NBP2-19852-img0006.jpg)
![Western Blot: PKM2 Antibody [NBP2-19852] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/PKM2-Antibody-Western-Blot-NBP2-19852-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: PKM2 Antibody [NBP2-19852] - PKM2 antibody detects PKM2 protein at cytoplasm by immunofluorescent analysis.<br>Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.<br>Green: PKM2 protein stained by PKM2 antibody (NBP2-19852) diluted at 1:500.<br>Blue: Hoechst 33342 staining.<br>](http://images.novusbio.com/fullsize/nbp2-19852_rabbit-polyclonal-pkm2-antibody-910202419593528.jpg)
![Immunohistochemistry-Paraffin: PKM2 Antibody [NBP2-19852] - PKM2 antibody detects PKM2 protein at cytoplasm by immunohistochemical analysis.</br>Sample: Paraffin-embedded human lung cancer.</br>PKM2 stained by PKM2 antibody (NBP2-19852) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19852_rabbit-polyclonal-pkm2-antibody-91020242014694.jpg)
![Western Blot: PKM2 Antibody [NBP2-19852] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM2 antibody (NBP2-19852) diluted at 1:5000.](http://images.novusbio.com/fullsize/nbp2-19852_rabbit-polyclonal-pkm2-antibody-91020242011489.jpg)
![Western Blot: PKM2 Antibody (GT1810) [NBP3-13632] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PKM2-Antibody-GT1810-Western-Blot-NBP3-13632-img0002.jpg "Western Blot: PKM2 Antibody (GT1810) [NBP3-13632] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody (GT1810) [NBP3-13632] - PKM2 antibody [GT1810] detects PKM2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKM2 stained by PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/PKM2-Antibody-GT1810-Immunocytochemistry-Immunofluorescence-NBP3-13632-img0003.jpg)
![Immunohistochemistry-Paraffin: PKM2 Antibody (GT1810) [NBP3-13632] - Human lung cancer. PKM2 stained by PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PKM2-Antibody-GT1810-Immunohistochemistry-Paraffin-NBP3-13632-img0004.jpg)
![Western Blot: PKM2 Antibody (GT1810) [NBP3-13632] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PKM2-Antibody-GT1810-Western-Blot-NBP3-13632-img0001.jpg)
![Immunohistochemistry: PKM1 Antibody (PSH04-37) [NBP3-32825] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-PKM1 antibody (NBP3-32825) at 1/30,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32825) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32825_rabbit-pkm1-mab-psh04-37-298202414262689.jpg "Immunohistochemistry: PKM1 Antibody (PSH04-37) [NBP3-32825] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-PKM1 antibody (NBP3-32825) at 1/30,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32825) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: PKM1 Antibody (PSH04-37) [NBP3-32825] - Immunocytochemistry analysis of HCT 116 cells labeling PKM1 with Rabbit anti-PKM1 antibody (NBP3-32825) at 1/200 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PKM1 antibody (NBP3-32825) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32825_rabbit-pkm1-mab-psh04-37-29202410234284.jpg)
![Flow Cytometry: PKM1 Antibody (PSH04-37) [NBP3-32825] - Flow cytometric analysis of HCT 116 cells labeling PKM1.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32825, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32825_rabbit-pkm1-mab-psh04-37-2920241637219.jpg)
![Western blot analysis on different lysates with NBP3-32825 at 1/1,000 dilution. Lane 1: HCT 116 cell lysate(20 µg/Lane) Lane 2: U-87 MG cell lysate(20 µg/Lane) Lane 3: HeLa cell lysate(20 µg/Lane) Lane 4: human brain tissue lysate(40 µg/Lane) Lane 5: mouse brain tissue lysate(40 µg/Lane) Lane 6: mouse heart tissue lysate(40 µg/Lane) Lane 7: mouse liver tissue lysate(40 µg/Lane) Low expression: mouse liver Exposure time: 14 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP3-32825, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 58 kDa Observed band size:58 kDa](http://images.novusbio.com/fullsize/nbp3-32825_-western-blot-639204597486750769.jpg)
![ELISA: Human PKM2 ELISA Kit (Colorimetric) [NBP3-18036] - Samples were spiked with high concentrations of Human PKM2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-18036_human-pkm2-elisa-kit-colorimetric-13220241625401.jpg "ELISA: Human PKM2 ELISA Kit (Colorimetric) [NBP3-18036] - Samples were spiked with high concentrations of Human PKM2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human PKM2 ELISA Kit (Colorimetric) [NBP3-18036] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-18036_human-pkm2-elisa-kit-colorimetric-4320249124848.jpg)
![Western Blot: PKM2 Antibody (SR2031) [NBP3-21688] - Western blot analysis using NBP3-21688 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21688_rabbit-pkm2-mab-sr2031-5720231558487.jpg "Western Blot: PKM2 Antibody (SR2031) [NBP3-21688] - Western blot analysis using NBP3-21688 in HeLa cell lysate.")
![Immunohistochemistry: PKM2 Antibody (SR2031) [NBP3-21688] - Immunohistochemical analysis of paraffin-embedded human cervix cancer, using NBP3-21688.](http://images.novusbio.com/fullsize/nbp3-21688_rabbit-pkm2-mab-sr2031-57202317215152.jpg)
