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HLA C Antibody (DT-9) - BSA Free

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, IP, CyTOF-ready
Clone
DT-9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-50419AF350
Alexa Fluor 405
NBP2-50419AF405
Alexa Fluor 488
NBP2-50419AF488
Alexa Fluor 532
NBP2-50419AF532
Alexa Fluor 594
NBP2-50419AF594
Alexa Fluor 647
NBP2-50419AF647
Alexa Fluor 700
NBP2-50419AF700
Alexa Fluor 750
NBP2-50419AF750
Allophycocyanin
NBP2-50419APC
Allophycocyanin/Cy7
NBP2-50419APCCY7
DyLight 350
NBP2-50419UV
DyLight 405
NBP2-50419V
DyLight 488
NBP2-50419G
DyLight 550
NBP2-50419R
DyLight 594
NBP2-50419DL594
DyLight 650
NBP2-50419C
DyLight 680
NBP2-50419FR
DyLight 755
NBP2-50419IR
FITC
NBP2-50419F
Janelia Fluor 549
NBP2-50419JF549
Janelia Fluor 646
NBP2-50419JF646
PE
NBP2-50419PE
PE/Atto594
NBP2-50419PEATT594
PE/Cy5.5
NBP2-50419PECY55
PE/Cy7
NBP2-50419PECY7
PerCP
NBP2-50419PCP
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-50419-0.025ml
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HLA C Antibody (DT-9) - BSA Free Summary

Immunogen
Purified tamarin MHC class I molecules (using anti-class I antibody BB7.7)
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
HLA-C
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:10 - 1:1000
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot
Application Notes
Use in Western Blot reported in scientific literature (PMID:23473775).DT9 has been reported to recognize HLA-C but not HLA-A or HLA-B allotypes (Braud et al., 1998). This antibody is CyTOF ready.
Publications
Read Publications using
NBP2-50419 in the following applications:

  • FACS
    2 publications
  • IP
    1 publication
  • WB
    1 publication

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:23473775).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for HLA C Antibody (DT-9) - BSA Free

  • C alpha chain
  • D6S204
  • FLJ27082
  • HLA class I histocompatibility antigen, Cw-1 alpha chain
  • HLAC
  • HLA-C
  • HLA-Cw
  • HLA-JY3
  • HLC-C
  • human leukocyte antigen-C alpha chain
  • leucocyte antigen C
  • leucocyte antigen Cw
  • leukocyte antigen-C alpha chain
  • major histocompatibility antigen HLA-C
  • major histocompatibility complex, class I, C
  • MHC class I antigen Cw*1
  • MHC class I antigen Cw*12
  • MHC class I antigen Cw*14
  • MHC class I antigen Cw*15
  • MHC class I antigen Cw*16
  • MHC class I antigen Cw*17
  • MHC class I antigen Cw*2
  • MHC class I antigen Cw*3
  • MHC class I antigen Cw*4
  • MHC class I antigen Cw*5
  • MHC class I antigen Cw*6
  • MHC class I antigen Cw*7
  • MHC class I antigen Cw*8
  • MHC class I antigen heavy chain HLA-C
  • PSORS1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis using the FITC conjugate of NB100-64775. Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control FITC (NBP1-43955) (blue histogram) or Anti-Human HLA-ABC FITC (purple histogram). Cells in the lymphocyte gate were used f
Immunohistochemistry-Frozen: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis of Mouse colon cancer tissue using HLA ABC antibody (W6/32). Primary antibody dilution was 1:100. Sample was incubated with secondary antibody in 1:300 dilution. Image from verified customer review.
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Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).
Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC12 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MHC Class I Antibody (OX18) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - Major histocompatibility complex (MHC) I and II as well as Transporter associated with antigen presentation II (TAPII) were analyzed, using immunocytochemistry on rat Schwann cells (SCs). Corresponding merges are shown in the bottom rows. Treatment of SCs with IL-17 was performed at concentrations of 0.5 and 50 ng/mL. Graphs to the right show densitometry quantification. SCs showed expression of MHCI > TAPII > MHCII, which increased after IL-17 treatment. MHCI was mainly detected in the cytoplasm and the expression increased in a dose-dependent manner after IL-17 treatment, significant for 0.5 ng/mL and 50 ng/mL (**P <=0.01). Image collected and cropped by CiteAb from the following publication (http://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-11-63), licensed under a CC-BY licence.
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Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.Both antibodies were conjugated to phycoerythrin.
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Western Blot: TRAILR2/TNFRSF10B Antibody [NB100-56618] - TRAIL R2/TNFRSF10B Antibody [NB100-56618] - Analysis using the Azide Free version of NB100-56618. Detection of 20 ug of whole cell lysates from HL60 cells with anti-TRAIL-R2 at 5 ug/ml.
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CTLA-4
[Unconjugated]
7268-CT
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Publications for HLA C Antibody (NBP2-50419)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: FACS, IP, WB.


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(2)
IP
(1)
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(1)
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(1)
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Bioinformatics

Gene Symbol HLA-C