HLA C Antibody (DT-9) - BSA Free

• Reactivity: Hu
• Applications: Flow, IP, CyTOF-ready
• Clone: DT-9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.9 mg/ml

HLA C Antibody (DT-9) - BSA Free Summary

• Immunogen: Purified tamarin MHC class I molecules (using anti-class I antibody BB7.7)
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-C
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10 - 1:1000
  • Immunoprecipitation 1:10 - 1:500
• Application Notes: This antibody is CyTOF ready.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 0.9 mg/ml
• Purity: Protein A purified

Alternate Names for HLA C Antibody (DT-9) - BSA Free

• C alpha chain
• D6S204
• FLJ27082
• HLA class I histocompatibility antigen, Cw-1 alpha chain
• HLAC
• HLA-C
• HLA-Cw
• HLA-JY3
• HLC-C
• human leukocyte antigen-C alpha chain
• leucocyte antigen C
• leucocyte antigen Cw
• leukocyte antigen-C alpha chain
• major histocompatibility antigen HLA-C
• major histocompatibility complex, class I, C
• MHC class I antigen Cw*1
• MHC class I antigen Cw*12
• MHC class I antigen Cw*14
• MHC class I antigen Cw*15
• MHC class I antigen Cw*16
• MHC class I antigen Cw*17
• MHC class I antigen Cw*2
• MHC class I antigen Cw*3
• MHC class I antigen Cw*4
• MHC class I antigen Cw*5
• MHC class I antigen Cw*6
• MHC class I antigen Cw*7
• MHC class I antigen Cw*8
• MHC class I antigen heavy chain HLA-C
• PSORS1

Background

HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HLA C Antibody (NBP2-50419)(5)

Publications using NBP2-50419

• Huang L, Malu S, McKenzie JA et al. The RNA-binding protein MEX3B mediates resistance to cancer immunotherapy by downregulating HLA-A expression. Clin. Cancer Res. 2018-03-01 [PMID: 29496759] (Human)
• Apps R, Qi Y, Carlson JM et al. Influence of HLA-C expression level on HIV control. Science. [PMID: 23559252] (FACS)
• Neef S, Sag C M et al. While systolic cardiomyocyte function is preserved, diastolic myocyte function and recovery from acidosis are impaired in CaMKII delta-KO mice. J Mol Cell Cardiol 2013-01-06 [PMID: 23473775] (WB, Mouse)
• Corrah TW, Goonetilleke N, Kopycinski J et al. Reappraisal of the relationship between the HIV-1-protective single-nucleotide polymorphism 35 kilobases upstream of the HLA-C gene and surface HLA-C expression. J Virol. [PMID: 21248048] (FACS)
• Braud VM, Allan DS, Wilson D, McMichael AJ. TAP- and tapasin-dependent HLA-E surface expression correlates with the binding of an MHC class I leader peptide. Curr Biol. [PMID: 9427624] (IP)

Bioinformatics

• Gene Symbol: HLA-C