Home » HLA C » HLA C Antibodies » HLA C Antibody (DT-9)

HLA C Antibody (DT-9)

 (4 Publications)
 See All Related
Submit a Review

Images

 
There are currently no images for HLA C Antibody (NBP2-50419).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IP, CyTOF-ready
Clone
DT-9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-50419AF350
Alexa Fluor 405
NBP2-50419AF405
Alexa Fluor 488
NBP2-50419AF488
Alexa Fluor 532
NBP2-50419AF532
Alexa Fluor 594
NBP2-50419AF594
Alexa Fluor 647
NBP2-50419AF647
Alexa Fluor 700
NBP2-50419AF700
Alexa Fluor 750
NBP2-50419AF750
Allophycocyanin
NBP2-50419APC
Allophycocyanin/Cy7
NBP2-50419APCCY7
DyLight 350
NBP2-50419UV
DyLight 405
NBP2-50419V
DyLight 488
NBP2-50419G
DyLight 550
NBP2-50419R
DyLight 594
NBP2-50419DL594
DyLight 650
NBP2-50419C
DyLight 680
NBP2-50419FR
DyLight 755
NBP2-50419IR
FITC
NBP2-50419F
Janelia Fluor 549
NBP2-50419JF549
Janelia Fluor 646
NBP2-50419JF646
PE
NBP2-50419PE
PE/Atto594
NBP2-50419PEATT594
PE/Cy5.5
NBP2-50419PECY55-100ul
PE/Cy7
NBP2-50419PECY7
PerCP
NBP2-50419PCP
100% Guarantee on every product

HLA C Antibody (DT-9) Summary

Immunogen
Purified tamarin MHC class I molecules (using anti-class I antibody BB7.7)
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
HLA-C
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10 - 1:1000
  • Immunoprecipitation 1:10 - 1:500
  • CyTOF-ready
Application Notes
DT9 has been reported to recognize HLA-C but not HLA-A or HLA-B allotypes (Braud et al., 1998). This antibody is CyTOF ready.
Publications
Read Publications using
NBP2-50419 in the following applications:

  • FACS
    2 publications
  • IP
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for HLA C Antibody (DT-9)

  • C alpha chain
  • D6S204
  • FLJ27082
  • HLA class I histocompatibility antigen, Cw-1 alpha chain
  • HLAC
  • HLA-Cw
  • HLA-JY3
  • HLC-C
  • human leukocyte antigen-C alpha chain
  • leucocyte antigen C
  • leucocyte antigen Cw
  • leukocyte antigen-C alpha chain
  • major histocompatibility antigen HLA-C
  • major histocompatibility complex, class I, C
  • MHC class I antigen Cw*1
  • MHC class I antigen Cw*12
  • MHC class I antigen Cw*14
  • MHC class I antigen Cw*15
  • MHC class I antigen Cw*16
  • MHC class I antigen Cw*17
  • MHC class I antigen Cw*2
  • MHC class I antigen Cw*3
  • MHC class I antigen Cw*4
  • MHC class I antigen Cw*5
  • MHC class I antigen Cw*6
  • MHC class I antigen Cw*7
  • MHC class I antigen Cw*8
  • MHC class I antigen heavy chain HLA-C
  • PSORS1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

HLA B Antibody
(2G7B10)
NBP2-61871
Western Blot: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis using HLA-B mAb against HEK293 (1) and HLA-B (AA: 241-362)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of Hela cells using HLA-B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry-Paraffin: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of bladder cancer tissues using HLA-B mouse mAb with DAB staining.
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
HLA ABC Antibody
(W6/32)
NB100-64775
Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis using the FITC conjugate of NB100-64775. Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control FITC (NBP1-43955) (blue histogram) or Anti-Human HLA-ABC FITC (purple histogram). Cells in the lymphocyte gate were used f
Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC.
Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 488.
(1 Review)
(23 Publications)
Species: Hu, Bb, Bv, Pm
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP, IHC-P (-)
HLA DRB4 Antibody
(4C8)
H00003126-M01
Western Blot: HLA DRB4 Antibody (4C8) [H00003126-M01] - HLA-DRB4 monoclonal antibody (M01), clone 4C8. Analysis of HLA-DRB4 expression in human kidney.
Immunohistochemistry-Paraffin: HLA DRB4 Antibody (4C8) [H00003126-M01] - Analysis of monoclonal antibody to HLA-DRB4 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1 ug/ml.
Sandwich ELISA: HLA DRB4 Antibody (4C8) [H00003126-M01] - Detection limit for recombinant GST tagged HLA-DRB4 is approximately 1ng/ml as a capture antibody.
(1 Publication)
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
MHC Class I
Antibody (OX18)
NB120-6405
Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-MHC Class I (OX18) NB120-6405 at a 1:100 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: MHC Class I Antibody (OX18) [NB120-6405] - Analysis of FFPE rat brain cerebellum using MHC Class I (OK18) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Endothelial staining was observed. Staining was performed by Histowiz.
Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).
(29 Publications)
Species: Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, CyTOF-ready, Flow-IC
KIR3DL1 Antibody
(177407)
MAB12251
KIR3DL1 Antibody (177407)
(3 Publications)
Species: Hu
Applications: Flow, CyTOF-ready
GEM Antibody
NBP1-58906
Western Blot: GEM Antibody [NBP1-58906] - Sample Tissue: Human Stomach Tumor Antibody Dilution: 1.0 ug/ml
Immunohistochemistry: GEM Antibody [NBP1-58906] - Paraffin Embedded Tissue: Human Kidney Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0 - 8.0 ug/ml Magnification: 400X
Western Blot: GEM Antibody [NBP1-58906] - Titration: 2.5ug/ml Positive Control: HepG2 cell lysate.
(2 Publications)
Species: Hu, Mu, Rt, Bv, Ca, Eq, GP, Rb
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
HLA DRB1 Antibody
(DA2)
NB100-2705
HLA DRB1 Antibody (DA2)
Species: Hu
Applications: WB, ELISA, Flow, IHC, CyTOF-ready
hnRNP C1 + C2
Antibody (SN0652)
NBP2-67656
Western Blot: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of hnRNP C1+C2 on different lysates using anti-hnRNP C1+C2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: HepG2
Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in B16-F1 (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of paraffin-embedded mouse skin tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt, Ze
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
HLA G Antibody
(87G)
NBP1-43123
Immunocytochemistry/Immunofluorescence: HLA G Antibody (87G) [NBP1-43123] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti HLA G (87G) at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow Cytometry: HLA G Antibody (87G) [NBP1-43123] - An intracellular stain was performed on HeLa cells with HLA G [87G] Antibody NBP1-43123AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: HLA G Antibody (87G) [NBP1-43123] - Staining of stimulated U937 cells with 0.25 ug of Mouse IgG2a K Isotype Control Purified (blue) or 0.25 ug of Anti-Human HLA-G Purified (purple) followed by F(ab')2 Anti-Mouse IgG PE. Total viable cells were used for analysis.
(6 Publications)
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, B/N, CyTOF-ready
TNF-alpha
[Unconjugated]
210-TA
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
(24 Reviews)
(606 Publications)
Species: Hu
KIR2DL3/CD158b2
Antibody (180701) [Un ...
MAB2014
Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human KIR2DL3/CD158b2 Monoclonal Antibody (Catalog # MAB2014) or (B) Isotype Control Antibody (Catalog # MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B) and Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P).View our protocol for Staining Membrane-associated Proteins.
(1 Review)
(7 Publications)
Species: Hu
Applications: Flow, CyTOF-reported
Recombinant Human
HLA DRB3 Protein
H00003125-P01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
HLA DRB5 Antibody
NBP2-14093
Western Blot: HLA DRB5 Antibody [NBP2-14093] - Analysis in human cell line HDLM-2.
Immunohistochemistry-Paraffin: HLA DRB5 Antibody [NBP2-14093] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.
Species: Hu
Applications: WB, IHC, IHC-P
KIR2DL1/KIR2DS5
Antibody (143211) [Un ...
MAB1844
Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human KIR2DL1/KIR2DS5 Monoclonal Antibody (Catalog # MAB1844) or (B) Mouse IgG1 Isotype Control Antibody (Catalog # MAB002) followed by Anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P). View our protocol for Staining Membrane-associated Proteins.
(11 Publications)
Species: Hu
Applications: Flow, CyTOF-reported
Corneodesmosin
Antibody
AF5725
Western blot shows lysates of NCI‑N87 human gastric carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Corneodesmosin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5725) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Corneodesmosin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Corneodesmosin was detected in immersion fixed paraffin-embedded sections of human skin using Sheep Anti-Human Corneodesmosin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5725) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(3 Publications)
Species: Hu
Applications: WB, IHC
Rat Complement
Component C2 ELISA Ki ...
NBP2-70034
Rat Complement Component C2 ELISA Kit (Colorimetric) - Standard Curve Reference
Species: Rt
HLA DQ Antibody
(SPV-L3)
NBP2-45041
Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
(1 Publication)
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF
NOD2 Antibody (2D9)
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
(1 Review)
(16 Publications)
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
TRAILR2/TNFRSF10B
Antibody
NB100-56618
Western Blot: TRAIL R2/TNFRSF10B Antibody [NB100-56618] - Total protein from HL-60, HepG2, HCT-116 and human placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TRAIL R2 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: TRAIL R2/TNFRSF10B Antibody [NB100-56618] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-TRAIL R2 (H76) at 5ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: TRAIL R2/TNFRSF10B Antibody [NB100-56618] - Analysis of a formalin fixed paraffin-embedded (FFPE) human brain cerebellum using 1:200 conc. of TRAIL R2/TNFRSF10B antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 30 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 9.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 15 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Cytoplasmic staining was observed in the Purkinje cell layer.
(1 Review)
(37 Publications)
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, Flow-CS
CTLA-4
[Unconjugated]
7268-CT
Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) inhibits IL-2 secretion by stimulated Jurkat human acute Tcell leukemia cells. The ED50 for this effect is 0.03-0.15 μg/mL whenstimulated with 1 μg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1).
(3 Reviews)
(1 Publication)
Species: Hu

Publications for HLA C Antibody (NBP2-50419)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: FACS, IP.


Filter By Application
FACS
(2)
IP
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for HLA C Antibody (NBP2-50419) (0)

There are no reviews for HLA C Antibody (NBP2-50419). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for HLA C Antibody (NBP2-50419) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-50419AF350
Alexa Fluor 405 NBP2-50419AF405
Alexa Fluor 488 NBP2-50419AF488
Alexa Fluor 532 NBP2-50419AF532
Alexa Fluor 594 NBP2-50419AF594
Alexa Fluor 647 NBP2-50419AF647
Alexa Fluor 700 NBP2-50419AF700
Alexa Fluor 750 NBP2-50419AF750
Allophycocyanin NBP2-50419APC
Allophycocyanin/Cy7 NBP2-50419APCCY7
DyLight 350 NBP2-50419UV
DyLight 405 NBP2-50419V
DyLight 488 NBP2-50419G
DyLight 550 NBP2-50419R
DyLight 594 NBP2-50419DL594
DyLight 650 NBP2-50419C
DyLight 680 NBP2-50419FR
DyLight 755 NBP2-50419IR
FITC NBP2-50419F
Janelia Fluor 549 NBP2-50419JF549
Janelia Fluor 646 NBP2-50419JF646
PE NBP2-50419PE
PE/Atto594 NBP2-50419PEATT594
PE/Cy5.5 NBP2-50419PECY55
PE/Cy7 NBP2-50419PECY7
PerCP NBP2-50419PCP
Learn about Custom Antibody Labeling

Additional HLA C Products

Bioinformatics Tool for HLA C Antibody (NBP2-50419)

Discover related pathways, diseases and genes to HLA C Antibody (NBP2-50419). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HLA C Antibody (NBP2-50419)

Discover more about diseases related to HLA C Antibody (NBP2-50419).
 

Pathways for HLA C Antibody (NBP2-50419)

View related products by pathway.

PTMs for HLA C Antibody (NBP2-50419)

Learn more about PTMs related to HLA C Antibody (NBP2-50419).
 

Research Areas for HLA C Antibody (NBP2-50419)

Find related products by research area.

Blogs on HLA C

There are no specific blogs for HLA C, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HLA C Antibody (DT-9) and receive a gift card or discount.


Bioinformatics

Gene Symbol HLA-C