Western Blot: PINK1 Antibody [NB600-973] - Lane 1: Mouse liver 100000Xg pellet (30ug). It recognizes primarily the full length protein at about 66 kDa in human, murine, and rat tissues. In addition, a truncated form of ...read more
Hu, Mu, RtSpecies Glossary
WB, IHC, IHC-P
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PINK1 (PTEN-induced putative kinase 1) is a serine/threonine protein kinase that localises to mitochondria. It has been implicated in protecting cells from stress-induced mitochondrial dysfunction. Mutations in this gene are thought to be the cause of autosomal recessive early-onset Parkinson disease 6 (PARK6).Phosphatase and Tensin Homolog (PTEN) dephosphorylates lipids such as phosphatidylinositol 3,4,5-triphosphate (PIP3) and its defects contribute to a variety of human cancers. PTEN induced putative kinase 1 (PINK1) was first identified when studying the tumor-suppressive function of the PTEN signaling pathway and is thus believed to be involved in human cancer pathology.1 The mRNA of PINK1 is expressed ubiquitously among adult tissues with most abundant expression in the heart, skeletal muscle, and testis.1 PINK1 is located in mitochondria and its homozygous C-terminus mutation is associated with early onset of Parkinson's disease.2 PINK1 Polyclonal Antibody recognizes primarily the full length protein at about 66 kDa in human, mouse, and rat tissues. In addition, a truncated form of the protein at about 33 kDa is also detected
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for PINK1 Antibody (NB600-973). (Showing 1 - 1 of 1 FAQs).
I bought PINK1 Antibody (NB600-973) from your company. According to data sheet, PINK1 polyclonal antibody recognizes primarily the full length protein at about 66 kDa in addition to about truncated 33 kDa based on western blot. Please could you tell me how immunohistochemistry could differentiate the two forms of protein? So, please could you tell me the subcelluar localization of PINK1? Could be nuclear under certain conditions? I know that PINK1 is mitochondrial protein, however, is the pattern of this protein is diffuse cytoplasmic or punctuate?
In answer to your question, because the antibody binds to both forms, and Western blot distinguishes between the two by separating by size (gel electrophoresis), which immunohistochemistry does not do, IHC would not differentiate between the full-length and truncated forms. The following journal article discusses the subcellular and submitochondrial localization of PINK1, so hopefully will be of use to you: The kinase domain of mitochondrial PINK1 faces the cytoplasm.
PINK1: All work and no fun The protein PINK1 is a mitochondrial-located serine/threonine kinase (PTK) that maintains organelle function and integrity. It not only protects organelles from cellular stress, but it also uses the selective auto-phagocytosis process for cleaning and... Read full blog post.
PINK1 and its role in Parkinson's disease PINK1 (PTEN induced putative kinase 1) is a mitochondrial serine/threonine kinase which maintains mitochondrial function/integrity, provides protection against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondr... Read full blog post.
PINK1: A Critical Player in Mitophagy PINK1 (PTEN-induced putative kinase 1) is a mitochondrial directed serine-threonine kinase, that regulates normal mitochondrial function and transport vital to normal performance of neurons and neuronal survival. PINK1 has been shown to be localized t... Read full blog post.