PINK1 Antibody

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Western Blot: PINK1 Antibody [NB600-973] - Lane 1: Mouse liver 100000Xg pellet (30ug). It recognizes primarily the full length protein at about 66 kDa in human, murine, and rat tissues. In addition, a truncated form of ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PINK1 Antibody Summary

Immunogen
Synthetic peptide from human PINK1, corresponding to amino acids 484-504 Human:LVRALLQREASKRPSARVAAN
Mouse:LVRsLLQREASKRPSARVAAN
Localization
Mitochondrial
Specificity
PINK1 polyclonal antibody recognizes primarily the full length protein at about 66 kDa in human, mouse, and rat tissues. In addition, a truncated form of the protein at about 33 kDa is also detected.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PINK1
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:150
  • Immunohistochemistry 4ug/ml
  • Immunohistochemistry-Paraffin 4ug/ml
Application Notes
This antibody is useful for Western Blot and Immunohistochemistry of formalin-fixed paraffin-embedded sections
Theoretical MW
63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Liver Lysate (NB820-59232)
Liver Lysate (NB820-59662)
Liver Lysate (NB820-59710)
Publications
Read Publications using
NB600-973 in the following applications:

Reactivity Notes

Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
No Preservative
Purity
Peptide affinity purified

Alternate Names for PINK1 Antibody

  • BRPK
  • EC 2.7.11.1
  • FLJ27236
  • PARK6
  • Parkinson disease (autosomal recessive) 6
  • PINK1
  • protein kinase BRPK
  • PTEN induced putative kinase 1
  • PTEN-induced putative kinase protein 1
  • serine/threonine-protein kinase PINK1, mitochondrial

Background

PINK1 (PTEN-induced putative kinase 1) is a serine/threonine protein kinase that localises to mitochondria. It has been implicated in protecting cells from stress-induced mitochondrial dysfunction. Mutations in this gene are thought to be the cause of autosomal recessive early-onset Parkinson disease 6 (PARK6).Phosphatase and Tensin Homolog (PTEN) dephosphorylates lipids such as phosphatidylinositol 3,4,5-triphosphate (PIP3) and its defects contribute to a variety of human cancers. PTEN induced putative kinase 1 (PINK1) was first identified when studying the tumor-suppressive function of the PTEN signaling pathway and is thus believed to be involved in human cancer pathology.1 The mRNA of PINK1 is expressed ubiquitously among adult tissues with most abundant expression in the heart, skeletal muscle, and testis.1 PINK1 is located in mitochondria and its homozygous C-terminus mutation is associated with early onset of Parkinson's disease.2 PINK1 Polyclonal Antibody recognizes primarily the full length protein at about 66 kDa in human, mouse, and rat tissues. In addition, a truncated form of the protein at about 33 kDa is also detected

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PINK1 Antibody (NB600-973)(2)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: IHC-Fr, IHC-P.


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IHC-Fr
(1)
IHC-P
(1)
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(1)
Rat
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PINK1 Antibody (NB600-973). (Showing 1 - 1 of 1 FAQs).

  1. I bought PINK1 Antibody (NB600-973) from your company. According to data sheet, PINK1 polyclonal antibody recognizes primarily the full length protein at about 66 kDa in addition to about truncated 33 kDa based on western blot. Please could you tell me how immunohistochemistry could differentiate the two forms of protein? So, please could you tell me the subcelluar localization of PINK1? Could be nuclear under certain conditions? I know that PINK1 is mitochondrial protein, however, is the pattern of this protein is diffuse cytoplasmic or punctuate?
    • In answer to your question, because the antibody binds to both forms, and Western blot distinguishes between the two by separating by size (gel electrophoresis), which immunohistochemistry does not do, IHC would not differentiate between the full-length and truncated forms. The following journal article discusses the subcellular and submitochondrial localization of PINK1, so hopefully will be of use to you: The kinase domain of mitochondrial PINK1 faces the cytoplasm.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on PINK1. Showing 1-10 of 11 blog posts - Show all blog posts.

There's an autophagy for that!
By Christina Towers, PhDA critical mechanism that cells use to generate nutrients and fuel metabolism is through a process called autophagy.  This process is complex and involves over 20 different proteins, most of which are highly conserved acro...  Read full blog post.

The role of Parkin and autophagy in retinal pigment epithelial cell (RPE) degradation
The root of Parkinson’s disease (PD) points to a poorly regulated electron transport chain leading to mitochondrial damage, where many proteins need to work cohesively to ensure proper function.  The two key players of this pathway are PINK1,...  Read full blog post.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Parkin - Role in Mitochondrial Quality Control and Parkinson's Disease
Parkin/PARK2 is a cytosolic enzyme which gets recruited to cellular mitochondria damaged through depolarization, ROS or unfolded proteins accumulation, and exert protective effects by inducing mitophagy (mitochondrial autophagy). Parkin induces mit...  Read full blog post.

PINK1 - performing mitochondrial quality control and protecting against Parkinson’s disease
PTEN-induced putative kinase 1 (PINK1) is a serine/threonine kinase with important functions in mitochondrial quality control. Together with the Parkin protein, PINK1 is able to regulate the selective degradation of damaged mitochondria through aut...  Read full blog post.

PINK1: All work and no fun
The protein PINK1 is a mitochondrial-located serine/threonine kinase (PTK) that maintains organelle function and integrity. It not only protects organelles from cellular stress, but it also uses the selective auto-phagocytosis process for cleaning and...  Read full blog post.

PINK1 and its role in Parkinson's disease
PINK1 (PTEN induced putative kinase 1) is a mitochondrial serine/threonine kinase which maintains mitochondrial function/integrity, provides protection against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondr...  Read full blog post.

PINK1: Promoting Organelle Stability and Preventing Parkinson's disease
PINK1 is a protein serine/threonine kinase (PTK) that protects the organelles from cellular stress and controls selective autophagy to clear damage. Exner, et al. were among the first to report that PINK1 deficiency in humans was linked to autosomal r...  Read full blog post.

PINK1: Promoting Organelle Stability and Preventing Parkinson's disease
PINK1 is a protein serine/threonine kinase (PTK) that protects the organelles from cellular stress and controls selective autophagy to clear damage. Exner, et. al. were among the first to report that PINK1 deficiency in humans was linked to autosomal...  Read full blog post.

PINK1: Linking Mitochondrial Health and Parkinson's disease
Parkinson's disease is a degenerative disorder of the central nervous system, which involves the loss of dopaminergic neurons in the brain and gives rise to tremors, rigidity and slowness of movement. In the majority of cases there is no known cause;...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol PINK1
Entrez
Uniprot