UCH-L1/PGP9.5 Antibody (31A3)

Images

 
Western Blot: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (31A3)
Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with UCH-L1/PGP9.5 Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 ...read more
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Formalin-fixed, paraffin-embedded human brain stained with UchL1 antibody (PGP9.5) (1:500), peroxidase-conjugate and DAB chromogen. Staining ...read more
Flow Cytometry: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Flow Cytometric Analysis of T98G cells using UCH-L1/PGP9.5 Antibody (31A3)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Western Blot: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - analysis of UchL1 in 1) human, 2) mouse and 3) rat brain lysate using UchL1 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and ECL substrate ...read more
Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - analysis of PGP9.5 in rat mesenteric artery using anti-PGP9.5 antibody. Image from verified customer review.
Simple Western: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Simple Western lane view shows a specific band for PGP9.5 / UCHL-1 in 0.2 mg/ml of h. Cerebellum (left) and IMR-32 (right) lysate(s). This experiment was ...read more
Simple Western: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Electropherogram images of the corresponding Simple Western lane. PGP9.5 / UCHL-1 antibody was used at 10 ug/ml dilution of h. Cerebellum and IMR-32 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, ZeSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC
Clone
31A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

UCH-L1/PGP9.5 Antibody (31A3) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33130)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.
Immunogen
UCH-L1/PGP9.5 protein from brain (Uniprot: P09936)
Localization
Cytoplasmic, Endoplasmic Reticulum membrane
Marker
pan-Neuronal Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
UCHL1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry 0.5-1.0ug/ml
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Simple Western 10 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Control
UCH-L1/PGP9.5 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB600-1160 in the following applications:

Publications
Read Publications using
NB600-1160 in the following applications:

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 30377377).

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for UCH-L1/PGP9.5 Antibody (31A3)

  • EC 3.4.19.12
  • EC 6.-
  • Neuron cytoplasmic protein 9.5
  • PARK5
  • PGP 9.5
  • PGP9.5
  • PGP9.5Uch-L1
  • PGP95
  • ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
  • ubiquitin carboxyl-terminal hydrolase isozyme L1
  • ubiquitin C-terminal hydrolase
  • Ubiquitin thioesterase L1
  • UCHL1
  • UCH-L1

Background

UchL1 (ubiquitin C-terminal hydrolase), also known as PGP9.5 (protein gene product 9.5) and PARK5, is a neuronal biomarker and ubquitin system protein (reviewed in Day and Thompson, 2010). UchL1 is a highly conserved antibody and has shown that it is expressed in neurons and neuroendocrine cells in vertebrates where it comprises about 5-10% of soluble cytoplasmic proteins. A minor proportion of UchL1 in brain is tightly bound to the membrane. UchL1 antibody is also expressed in human oocytes and spermatogonia. It is important to note that the use of an antibody as a biomarker in this case does not imply that an antibody defines absolute tissue specificity. Rather, it means that PGP9.5 is expressed in neurons and neuroendrocine cells at significantly higher levels than in other cell types.
Functionally, UchL1 antibody is a thiol protease enzyme that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UchL1 antibody also binds to free monoubiquitin and inhibits monoubiquitin degradation in lysosomes; it may function to stabilize monoubiquitin within neurons. A mutation in the UchL1 gene has been found to cause a form of Parkinson's disease, and this discovery has spurred considerable research interest in UchL1 and to its alternative name of PARK5.
Antibody to UchL1 is widely used as an immunohistochemical marker of nerves and neuroendocrine cells. The UchL1 clone 31A3 antibody stains neuronal cell bodies and axons in the central and peripheral nervous systems as well as small nerve fibers in peripheral tissues, including epidermal tissues (Day & Thompson, 2010). The antibody also stains neuroendrocrine cells in the kidney, pituitary, thyroid, pancreas, gastrointestinal tract, and tumors of the diffuse neuroendocrine system. This antibody has also identified UchL1 expression in renal tubule spermatogonia, testis, ovary, and both pregnant and non-pregnant corpus luteum. In western blots, the antibody identifies UchL1 as a band of approximately 20-30 kDa. The antibody is specific to UchL1 and does not cross-react with the closely related UchL3 protein (Yi et al, 2007).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UCH-L1/PGP9.5 Antibody (NB600-1160)(15)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Zebrafish.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(3)
IF/IHC
(4)
IHC-Fr
(3)
IHC-P
(3)
WB
(2)
All Applications
Filter By Species
Human
(3)
Mouse
(4)
Rat
(3)
Zebrafish
(1)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB600-1160 Applications Species
Lucarini E, Micheli L, Pagnotta E et al. The Efficacy of Camelina sativa Defatted Seed Meal against Colitis-Induced Persistent Visceral Hypersensitivity: The Relevance of PPAR alpha Receptor Activation in Pain Relief Nutrients 2022-07-29 [PMID: 35956313] (IHC-P, Rat)

Details:
Dilution used 1:500
IHC-P Rat
Manavis J, Blumbergs P, Jerrett I et al. Heterogeneous immunoreactivity of axonal spheroids in focal symmetrical encephalomalacia produced by Clostridium perfringens type D epsilon toxin in sheep Veterinary pathology 2021-12-06 [PMID: 34872408]
Pal P, Hales K, Hales DB Increased Transient Receptor Potential Melastatin 8 Expression in the development of bladder pain in patients with Interstitial cystitis/Bladder Pain Syndrome Oncotarget 2020-10-10 [PMID: 33088425] (ICC/IF, Human) ICC/IF Human
Moutal A, Ji Y, Bellampalli SS, Khanna R Differential expression of Cdk5-phosphorylated CRMP2 following a spared nerve injury Mol Brain 2020-06-22 [PMID: 32571373] (IF/IHC, Rat) IF/IHC Rat
Arnaboldi F, Sommariva M, Opizzi E et al. Expression of Toll-like receptors 4 and 7 in murine peripheral nervous system development Ann. Anat. 2020-05-04 [PMID: 32380196] (ICC/IF, Mouse) ICC/IF Mouse
Alberio L, Locarno A, Saponaro A et al. A light-gated potassium channel for sustained neuronal inhibition. Nat. Methods. 2018-11-01 [PMID: 30377377] (IF/IHC, Zebrafish) IF/IHC Zebrafish
Sundaresan S, Meininger C A et al. Gastrin Induces Nuclear Export and Proteasome Degradation of Menin in Enteric Glial Cells. Gastroenterology 2017-01-12 [PMID: 28859856] (IF/IHC, ICC/IF, Mouse) IF/IHC, ICC/IF Mouse
Novakovic P, Harding JC, Al-Dissi AN et al. Pathologic Evaluation of Type 2 Porcine Reproductive and Respiratory Syndrome Virus Infection at the Maternal-Fetal Interface of Late Gestation Pregnant Gilts PLoS ONE 2016-03-11 [PMID: 26963101] (IF/IHC, WB, Human) IF/IHC, WB Human
Read NC, Gutsol A, Holterman CE et al. Ubiquitin C-terminal hydrolase L1 deletion ameliorates glomerular injury in mice with ACTN4-associated focal segmental glomerulosclerosis. Biochim. Biophys. Acta 2014-03-22 [PMID: 24662305] (IHC-P, Mouse) IHC-P Mouse
Bottner M, Zorenkov D, Hellwig I et al. Expression pattern and localization of alpha-synuclein in the human enteric nervous system. Neurobiol Dis. 2012-12-01 [PMID: 22850485] (IHC-Fr)

Details:
IHC (frozen); Table I (colon carcinoma, dysplastic adenoma).
IHC-Fr
Show All 15 Publications.

Review for UCH-L1/PGP9.5 Antibody (NB600-1160) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB600-1160:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry UCH-L1/PGP9.5 NB600-1160
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5
reviewed by:
Verified Customer
ICC Rat 03/26/2015
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Summary

ApplicationImmunocytochemistry
Sample TestedMesenteric artery-whole
SpeciesRat
Lot7306-1p140206

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for UCH-L1/PGP9.5 Antibody (NB600-1160) (0)

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Verified Customer
03/26/2015
Application: ICC
Species: Rat

Bioinformatics

Gene Symbol UCHL1
Entrez
Uniprot