UCH-L1/PGP9.5 Antibody (31A3)


Western Blot: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (31A3)
Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with UCH-L1/PGP9.5 Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 ...read more
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Formalin-fixed, paraffin-embedded human brain stained with UchL1 antibody (PGP9.5) (1:500), peroxidase-conjugate and DAB chromogen. Staining ...read more
Flow Cytometry: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Flow Cytometric Analysis of T98G cells using UCH-L1/PGP9.5 Antibody (31A3)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Western Blot: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - analysis of UchL1 in 1) human, 2) mouse and 3) rat brain lysate using UchL1 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and ECL substrate ...read more
Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - analysis of PGP9.5 in rat mesenteric artery using anti-PGP9.5 antibody. Image from verified customer review.
Simple Western: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Simple Western lane view shows a specific band for PGP9.5 / UCHL-1 in 0.2 mg/ml of h. Cerebellum (left) and IMR-32 (right) lysate(s). This experiment was ...read more
Simple Western: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Electropherogram images of the corresponding Simple Western lane. PGP9.5 / UCHL-1 antibody was used at 10 ug/ml dilution of h. Cerebellum and IMR-32 ...read more

Product Details

Reactivity Hu, Mu, Rt, Po, Bv, ZeSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
0.2 mg/ml

Order Details

UCH-L1/PGP9.5 Antibody (31A3) Summary

Native UchL1 (PGP9.5) protein from brain was used as immunogen to generate the antibody (Day & Thompson, 1986).
Cytoplasmic, Endoplasmic Reticulum membrane
pan-Neuronal Marker
This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.
IgG1 Kappa
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1-2 ug/ml
  • Simple Western 10 ug/ml
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Frozen 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Human Chromosome Location: 4p13
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined. This antibody has been reported to work in IHC-Frozen as reported in the literature (Bottner et al. Drew et al.) and (Sugimoto et al. J Hyperten).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
UCH-L1/PGP9.5 Overexpression Lysate
UCH-L1/PGP9.5 Knockout 293T Cell Lysate
Reviewed Applications
Read 1 Review rated 5
NB600-1160 in the following applications:

Read Publications using
NB600-1160 in the following applications:

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 30377377).

Packaging, Storage & Formulations

Store at 4C.
10 mM PBS with 0.05% BSA
0.05% Sodium Azide
0.2 mg/ml
Protein A or G purified

Alternate Names for UCH-L1/PGP9.5 Antibody (31A3)

  • EC
  • EC 6.-
  • Neuron cytoplasmic protein 9.5
  • PARK5
  • PGP 9.5
  • PGP9.5
  • PGP9.5Uch-L1
  • PGP95
  • ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
  • ubiquitin carboxyl-terminal hydrolase isozyme L1
  • ubiquitin C-terminal hydrolase
  • Ubiquitin thioesterase L1
  • UCHL1
  • UCH-L1


UchL1 (ubiquitin C-terminal hydrolase), also known as PGP9.5 (protein gene product 9.5) and PARK5, is a neuronal biomarker and ubquitin system protein (reviewed in Day and Thompson, 2010). UchL1 is a highly conserved antibody and has shown that it is expressed in neurons and neuroendocrine cells in vertebrates where it comprises about 5-10% of soluble cytoplasmic proteins. A minor proportion of UchL1 in brain is tightly bound to the membrane. UchL1 antibody is also expressed in human oocytes and spermatogonia. It is important to note that the use of an antibody as a biomarker in this case does not imply that an antibody defines absolute tissue specificity. Rather, it means that PGP9.5 is expressed in neurons and neuroendrocine cells at significantly higher levels than in other cell types.
Functionally, UchL1 antibody is a thiol protease enzyme that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UchL1 antibody also binds to free monoubiquitin and inhibits monoubiquitin degradation in lysosomes; it may function to stabilize monoubiquitin within neurons. A mutation in the UchL1 gene has been found to cause a form of Parkinson's disease, and this discovery has spurred considerable research interest in UchL1 and to its alternative name of PARK5.
Antibody to UchL1 is widely used as an immunohistochemical marker of nerves and neuroendocrine cells. The UchL1 clone 31A3 antibody stains neuronal cell bodies and axons in the central and peripheral nervous systems as well as small nerve fibers in peripheral tissues, including epidermal tissues (Day & Thompson, 2010). The antibody also stains neuroendrocrine cells in the kidney, pituitary, thyroid, pancreas, gastrointestinal tract, and tumors of the diffuse neuroendocrine system. This antibody has also identified UchL1 expression in renal tubule spermatogonia, testis, ovary, and both pregnant and non-pregnant corpus luteum. In western blots, the antibody identifies UchL1 as a band of approximately 20-30 kDa. The antibody is specific to UchL1 and does not cross-react with the closely related UchL3 protein (Yi et al, 2007).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UCH-L1/PGP9.5 Antibody (NB600-1160)(11)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Zebrafish.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.

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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB600-1160 Applications Species
Moutal A, Ji Y, Bellampalli SS, Khanna R Differential expression of Cdk5-phosphorylated CRMP2 following a spared nerve injury Mol Brain Jun 22 2020 [PMID: 32571373] (IHC, Rat) IHC Rat
Arnaboldi F, Sommariva M, Opizzi E et al. Expression of Toll-like receptors 4 and 7 in murine peripheral nervous system development Ann. Anat. May 4 2020 [PMID: 32380196] (ICC/IF, Mouse) ICC/IF Mouse
Alberio L, Locarno A, Saponaro A et al. A light-gated potassium channel for sustained neuronal inhibition. Nat. Methods. Nov 1 2018 [PMID: 30377377] (IHC, Zebrafish) IHC Zebrafish
Novakovic P, Harding JC, Al-Dissi AN et al. Pathologic Evaluation of Type 2 Porcine Reproductive and Respiratory Syndrome Virus Infection at the Maternal-Fetal Interface of Late Gestation Pregnant Gilts PLoS ONE 2016 Mar 11 [PMID: 26963101] (IHC, WB, Human) IHC, WB Human
Read NC, Gutsol A, Holterman CE et al. Ubiquitin C-terminal hydrolase L1 deletion ameliorates glomerular injury in mice with ACTN4-associated focal segmental glomerulosclerosis. Biochim. Biophys. Acta 3/22/2014 [PMID: 24662305] (IHC-P, Mouse) IHC-P Mouse
Bottner M, Zorenkov D, Hellwig I et al. Expression pattern and localization of alpha-synuclein in the human enteric nervous system. Neurobiol Dis. 2012 Dec [PMID: 22850485] (IHC-Fr)

IHC (frozen); Table I (colon carcinoma, dysplastic adenoma).
Sugimoto K, Kojima K, Baba M, Yasujima M. Olmesartan ameliorates peripheral nerve dysfunction in Zucker diabetic fatty rats. J Hypertens. 2011 Jul [PMID: 21602711] (IHC-Fr, Rat)

IHC (frozen); Fig 3 (rat skin).
IHC-Fr Rat
Day IN, Thompson RJ. UCHL1 (PGP 9.5): neuronal biomarker and ubiquitin system protein. Prog Neurobiol. 2010 Mar [PMID: 19879917] (IHC-P)

IHC (paraffin): Fig 2a,b (human brain). The distribution of PGP9.5 is extensively described in this review article, researchers are encouraged to consult this outstanding article for additional information.
Gaunitz C, Gabert J, Lucker E et al. Suitability of antigens PGP 9.5 and neurofilament light as marker proteins for detection of neuronal tissue in processed meat products. J Food Prot. 2009 May [PMID: 19517736]

Sandwich ELISA (cow and pig meat). The antibody was used in a sandwich ELISA along with the PGP9.5 13C4 antibody clone to detect neuronal contaminant components in meat. Refer to the publication for additional details.
Yi YJ, Manandhar G, Sutovsky M et al. Ubiquitin C-terminal hydrolase-activity is involved in sperm acrosomal function and anti-polyspermy defense during porcine fertilization. Biol Reprod. 2007 Nov [PMID: 17671268] (WB, Mouse)

WB: Fig 6a (mouse testicular cells and spermatozoa, boar testicular cells). UchL1 was detected just between the 19 and 26 kDa markers. Note: UchL1 was not detected in boar testicular cells by WB.
WB Mouse
Show All 11 Publications.

Review for UCH-L1/PGP9.5 Antibody (NB600-1160) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB600-1160:
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Images Ratings Applications Species Date Details
Immunocytochemistry UCH-L1/PGP9.5 NB600-1160
reviewed by:
ICC Rat 03/26/2015


Sample TestedMesenteric artery-whole


Blocking Details5% NDS, 1hr at room temperature


Detection NotesConfocal Microscopy, Zeiss Axiovison Observer Z.1 with spinning disk confocal, 20x, Tyrosine Hydroxlase-pos/no AB neg

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for UCH-L1/PGP9.5 Antibody (NB600-1160) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-33130AF350
Alexa Fluor 405 NBP2-33130AF405
Alexa Fluor 488 NBP2-33130AF488
Alexa Fluor 532 NBP2-33130AF532
Alexa Fluor 594 NBP2-33130AF594
Alexa Fluor 647 NBP2-33130AF647
Alexa Fluor 700 NBP2-33130AF700
Alexa Fluor 750 NBP2-33130AF750
Biotin NBP2-33130B
DyLight 350 NBP2-33130UV
DyLight 405 NBP2-33130V
DyLight 488 NBP2-33130G
DyLight 550 NBP2-33130R
DyLight 594 NBP2-33130DL594
DyLight 650 NBP2-33130C
DyLight 680 NBP2-33130FR
DyLight 755 NBP2-33130IR
FITC NBP2-33130F
HRP NBP2-33130H
Janelia Fluor 549 NBP2-33130JF549
Janelia Fluor 646 NBP2-33130JF646

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Recent Reviews


Application: ICC
Species: Rat


Gene Symbol UCHL1