UCH-L1/PGP9.5 Antibody (31A3)

Images

 
Western Blot: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - analysis of UchL1 in 1) human, 2) mouse and 3) rat brain lysate using UchL1 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and ECL substrate ...read more
Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - analysis of PGP9.5 in rat mesenteric artery using anti-PGP9.5 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Formalin-fixed, paraffin-embedded human brain stained with UchL1 antibody (PGP9.5) (1:500), peroxidase-conjugate and DAB chromogen. Staining ...read more
Simple Western: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Simple Western lane view shows a specific band for PGP9.5 / UCHL-1 in 0.2 mg/ml of h. Cerebellum (left) and IMR-32 (right) lysate(s). This experiment was ...read more
Simple Western: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Electropherogram images of the corresponding Simple Western lane. PGP9.5 / UCHL-1 antibody was used at 10 ug/ml dilution of h. Cerebellum and IMR-32 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, Simple Western, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
31A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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UCH-L1/PGP9.5 Antibody (31A3) Summary

Immunogen
Native UchL1 (PGP9.5) protein from brain was used as immunogen to generate the antibody (Day & Thompson, 1986).
Localization
Cytoplasmic, Endoplasmic Reticulum membrane
Marker
pan-Neuronal Marker
Specificity
This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
UCHL1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • Simple Western 10 ug/ml
  • ELISA 1-5ug/ml for coating
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 0.5-1.0ug/ml
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
Application Notes
Human Chromosome Location: 4p13
Fixation in 95% ethanol/5% acetic acid for 2-3 hours prior to paraffin embedding is recommended. Specimens which have not been fixed in acetic acid/alcohol require pre-treatment with citrate buffer: for example, unmask of the target epitope by boiling the tissue sections in 10mM citrate buffer, pH 6.0, for 10 min followed by cooling at room temp for 20 min. This antibody has been reported to work in IHC-Frozen as reported in the literature (Bottner et al. Drew et al.) and (Sugimoto et al. J Hyperten).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
UCH-L1/PGP9.5 Lysate (NBL1-17575)
UCH-L1/PGP9.5 Lysate (NBP2-65016)
Reviewed Applications
Read 1 Review rated 5
using
NB600-1160 in the following applications:

Publications
Read Publications using
NB600-1160 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for UCH-L1/PGP9.5 Antibody (31A3)

  • EC 3.4.19.12
  • EC 6.-
  • Neuron cytoplasmic protein 9.5
  • PARK5
  • PGP 9.5
  • PGP9.5
  • PGP9.5Uch-L1
  • PGP95
  • ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
  • ubiquitin carboxyl-terminal hydrolase isozyme L1
  • ubiquitin C-terminal hydrolase
  • Ubiquitin thioesterase L1
  • UCHL1
  • UCH-L1

Background

UchL1 (ubiquitin C-terminal hydrolase), also known as PGP9.5 (protein gene product 9.5) and PARK5, is a neuronal biomarker and ubquitin system protein (reviewed in Day and Thompson, 2010). UchL1 is a highly conserved antibody and has shown that it is expressed in neurons and neuroendocrine cells in vertebrates where it comprises about 5-10% of soluble cytoplasmic proteins. A minor proportion of UchL1 in brain is tightly bound to the membrane. UchL1 antibody is also expressed in human oocytes and spermatogonia. It is important to note that the use of an antibody as a biomarker in this case does not imply that an antibody defines absolute tissue specificity. Rather, it means that PGP9.5 is expressed in neurons and neuroendrocine cells at significantly higher levels than in other cell types.
Functionally, UchL1 antibody is a thiol protease enzyme that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UchL1 antibody also binds to free monoubiquitin and inhibits monoubiquitin degradation in lysosomes; it may function to stabilize monoubiquitin within neurons. A mutation in the UchL1 gene has been found to cause a form of Parkinson's disease, and this discovery has spurred considerable research interest in UchL1 and to its alternative name of PARK5.
Antibody to UchL1 is widely used as an immunohistochemical marker of nerves and neuroendocrine cells. The UchL1 clone 31A3 antibody stains neuronal cell bodies and axons in the central and peripheral nervous systems as well as small nerve fibers in peripheral tissues, including epidermal tissues (Day & Thompson, 2010). The antibody also stains neuroendrocrine cells in the kidney, pituitary, thyroid, pancreas, gastrointestinal tract, and tumors of the diffuse neuroendocrine system. This antibody has also identified UchL1 expression in renal tubule spermatogonia, testis, ovary, and both pregnant and non-pregnant corpus luteum. In western blots, the antibody identifies UchL1 as a band of approximately 20-30 kDa. The antibody is specific to UchL1 and does not cross-react with the closely related UchL3 protein (Yi et al, 2007).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UCH-L1/PGP9.5 Antibody (NB600-1160)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: IHC, IHC-Fr, IHC-P, WB.


Filter By Application
IHC
(1)
IHC-Fr
(3)
IHC-P
(2)
WB
(2)
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Human
(2)
Mouse
(2)
Rat
(1)
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Showing Publications 1 - 8 of 8.
Publications using NB600-1160 Applications Species
Novakovic P, Harding JC, Al-Dissi AN et al. Pathologic Evaluation of Type 2 Porcine Reproductive and Respiratory Syndrome Virus Infection at the Maternal-Fetal Interface of Late Gestation Pregnant Gilts PLoS ONE 2016 Mar 11 [PMID: 26963101] (IHC, WB, Human) IHC, WB Human
Read NC, Gutsol A, Holterman CE et al. Ubiquitin C-terminal hydrolase L1 deletion ameliorates glomerular injury in mice with ACTN4-associated focal segmental glomerulosclerosis. Biochim. Biophys. Acta 3/22/2014 [PMID: 24662305] (IHC-P, Mouse) IHC-P Mouse
Bottner M, Zorenkov D, Hellwig I et al. Expression pattern and localization of alpha-synuclein in the human enteric nervous system. Neurobiol Dis. 2012 Dec [PMID: 22850485] (IHC-Fr)

Details:
IHC (frozen); Table I (colon carcinoma, dysplastic adenoma).
IHC-Fr
Sugimoto K, Kojima K, Baba M, Yasujima M. Olmesartan ameliorates peripheral nerve dysfunction in Zucker diabetic fatty rats. J Hypertens. 2011 Jul [PMID: 21602711] (IHC-Fr, Rat)

Details:
IHC (frozen); Fig 3 (rat skin).
IHC-Fr Rat
Day IN, Thompson RJ. UCHL1 (PGP 9.5): neuronal biomarker and ubiquitin system protein. Prog Neurobiol. 2010 Mar [PMID: 19879917] (IHC-P)

Details:
IHC (paraffin): Fig 2a,b (human brain). The distribution of PGP9.5 is extensively described in this review article, researchers are encouraged to consult this outstanding article for additional information.
IHC-P
Gaunitz C, Gabert J, Lucker E et al. Suitability of antigens PGP 9.5 and neurofilament light as marker proteins for detection of neuronal tissue in processed meat products. J Food Prot. 2009 May [PMID: 19517736]

Details:
Sandwich ELISA (cow and pig meat). The antibody was used in a sandwich ELISA along with the PGP9.5 13C4 antibody clone to detect neuronal contaminant components in meat. Refer to the publication for additional details.
Yi YJ, Manandhar G, Sutovsky M et al. Ubiquitin C-terminal hydrolase-activity is involved in sperm acrosomal function and anti-polyspermy defense during porcine fertilization. Biol Reprod. 2007 Nov [PMID: 17671268] (WB, Mouse)

Details:
WB: Fig 6a (mouse testicular cells and spermatozoa, boar testicular cells). UchL1 was detected just between the 19 and 26 kDa markers. Note: UchL1 was not detected in boar testicular cells by WB.
WB Mouse
Stander S, Schmelz M, Metze D et al. Distribution of cannabinoid receptor 1 (CB1) and 2 (CB2) on sensory nerve fibers and adnexal structures in human skin. J Dermatol Sci. 2005 Jun [PMID: 15927811] (IHC-Fr, Human)

Details:
IHC (frozen); Fig 2E (human skin).
IHC-Fr Human

Review for UCH-L1/PGP9.5 Antibody (NB600-1160) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB600-1160:
Filter by Applications
ICC
(1)
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Rat
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry UCH-L1/PGP9.5 NB600-1160
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reviewed by:
Anonymous
ICC Rat 03/26/2015
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Summary

ApplicationImmunocytochemistry
Sample TestedMesenteric artery-whole
SpeciesRat
Lot7306-1p140206

Blocking

Blocking Details5% NDS, 1hr at room temperature

Details

Detection NotesConfocal Microscopy, Zeiss Axiovison Observer Z.1 with spinning disk confocal, 20x, Tyrosine Hydroxlase-pos/no AB neg

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for UCH-L1/PGP9.5 Antibody (NB600-1160) (0)

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Recent Reviews

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Anonymous
03/26/2015
Application: ICC
Species: Rat

Bioinformatics

Gene Symbol UCHL1
Entrez
Uniprot