DRP1 Antibody (NB110-55288)

Images

 
Western Blot: DRP1 Antibody [NB110-55288] - Total protein from human HeLa and MCF7 cells, mouse 3T3 cells and rat PC12 cells was separated on a 7.5 % gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% ...read more
Immunohistochemistry-Paraffin: DRP1 Antibody [NB110-55288] - IHC analysis of a formain fixed paraffin embedded (FFPE) tissue section of mouse kidney using DRP1 antibody #NB110-55288 at 1: 300 dilution. The primary ...read more
Immunohistochemistry: DRP1 Antibody [NB110-55288] - Staining of renal tubular epithelium and visceral epithelial cells of the glomerulus. Human kidney cortex, 40X magnification.
Western Blot: DRP1 Antibody [NB110-55288] - Lane 1: DRP1 knockout Lane 2: DRP1 wildtype MEFs Stained with NB110-55288 at 1:500x dilution.
Immunocytochemistry/ Immunofluorescence: DRP1 Antibody [NB110-55288] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Western Blot: DRP1 Antibody [NB110-55288] - Rat spinal cord-DRP1 (81kda). Image from verified customer review.
Simple Western: DRP1 Antibody [NB110-55288] - Simple Western lane view shows a specific band for DUX4 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Fi, Pm, BvSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
     

Genetic Strategies

   

DRP1 Antibody Summary

Immunogen
A synthetic peptide made to an internal region within residues 500-600 of the human DRP1 protein. [Swiss-Prot# O00429]
Localization
Cytoplasm; cytosol. Intracytoplasmic membrane; peripheral mambrane protein. Mainly cytosolic. Also membrane-associated. Localizes to mitochondia at spots of division events. Associated with peroxisomal membranes, it is recruited in part by PEX11B. May als
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNM1L
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:300-1:1000
  • Immunohistochemistry-Paraffin 1:300-1:1000
  • Immunoprecipitation
Application Notes
This DRP1 antibody is useful for Immonohistochemistry paraffin embedded sections, Immunocytochemistry/Immunofluorescence and Western blot analysis where a band is seen at approx. 81 kDa. In ICC/IF, cytoplasmic staining was observed in HeLa cells. Immunoprecipitation was reported in scientific literature.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Kidney Lysate (NB820-59231)
DRP1 Lysate (NBL1-09966)
Control Peptide
DRP1 Peptide (NB110-55288PEP)
Reviewed Applications
Read 2 Reviews rated 2.5
using
NB110-55288 in the following applications:

Publications
Read Publications using
NB110-55288 in the following applications:

  • 4 publications
  • IHC
    2 publications
  • 1 publication
  • IP
    2 publications
  • WB
    11 publications

Reactivity Notes

Human, primate, rat, mouse. Predicted to react with bovine based on 100% sequence homology. Fish reactivity reported in scientific literature (PMID: 25008790)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DRP1 Antibody

  • DLP1
  • DRP1Dnm1p/Vps1p-like protein
  • DVLPEC 3.6.5.5
  • Dymple
  • DYMPLEDYNIV-11
  • dynamin 1-like
  • Dynamin family member proline-rich carboxyl-terminal domain less
  • dynamin-1-like protein
  • Dynamin-like protein 4
  • Dynamin-like protein IV
  • Dynamin-like protein
  • Dynamin-related protein 1
  • FLJ41912
  • HDYNIV
  • VPS1

Background

DRP1 (dynamin-related protein 1) is a highly conserved large GTPase protein which implicates in several important structural features of mitochondria, including mitochondrial shape, size, distribution, remodeling and maintenance. It functions in mitochondrial as well as peroxisomal division, and facilitates membrane fission via oligomerization into ring-like structures that wrap around fission site to constrict/split the membranes through a GTP hydrolysis-dependent mechanism. For normal brain development, DRP1 plays an essential role by mediating developmentally-regulated apoptosis during neural tube development. It is also required for a normal rate of cytochrome c release or caspase activation during apoptosis and for mitochondrial fission during mitosis, programmed necrosis execution and also for vesicle transport. Moreover, it is associated with mitochondrial structural and functional alterations such as fragmentation, phosphorylation, SUMOylation, ubiquitination and cell death. Drp1 interacts with amyloid beta, causing abnormal mitochondrial dynamics and neuronal damage in Alzheimer's disease patients, and defective DRP1 is also linked to encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Fi, Pm, Bv
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP

Publications for DRP1 Antibody (NB110-55288)(16)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Fish.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ICC/IF
(4)
IHC
(2)
IHC-P
(1)
IP
(2)
WB
(11)
All Applications
Filter By Species
Human
(4)
Mouse
(6)
Rat
(3)
Fish
(1)
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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB110-55288 Applications Species
Wang L, Zhang T, Wang L et al. Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission. EMBO J. Apr 4 2017 [PMID: 28377463] (WB, Mouse) WB Mouse
Chan YL, Saad S, Al-Odat I et al. Maternal L-Carnitine Supplementation Improves Brain Health in Offspring from Cigarette Smoke Exposed Mothers. Front Mol Neurosci. 2017 Feb 28 [PMID: 28243190] (WB, Mouse) WB Mouse
Xu S, Wang P, Zhang H et al. CaMKII induces permeability transition through Drp1 phosphorylation during chronic B-AR stimulation. Nat Commun Oct 14 2016 [PMID: 27739424] (IP, WB, Rat) IP, WB Rat
Chen H, Chan YL, Nguyen LT et al. Moderate traumatic brain injury is linked to acute behaviour deficits and long term mitochondrial alterations. Clin. Exp. Pharmacol. Physiol. Aug 25 2016 [PMID: 27557565] (IHC, Rat) IHC Rat
Lennon FE, Cianci GC, Kanteti R et al. Unique fractal evaluation and therapeutic implications of mitochondrial morphology in malignant mesothelioma. Sci Rep. 2016 Apr 15 [PMID: 27080907] (WB, Human)

Details:
DRP1 antibody was used for WB analysis of total cell lysates as well as mitochondrial fractions from MeT-5A (control transformed non-tumorigenic mesothelial cells), and mesothelioma cell lines namely H28, H513, H2052, H2461 (Epithelioid) and H2373, H2596
WB Human
Zhang M, Sun T, Jian C et al. Remodeling of Mitochondrial Flashes in Muscular Development and Dystrophy in Zebrafish. PLoS One 2015 [PMID: 26186000] (ICC/IF) ICC/IF
Palubinsky AM, Stankowski JN, Kale AC et al. CHIP is an Essential Determinant of Neuronal Mitochondrial Stress Signaling Antioxid. Redox Signal. 2015 Jan 20 [PMID: 25602369] (WB, Mouse)

Details:
DRP1 antibody was used for WB application on whole brain lysates from PND35 - CHIP/C-terminus of HSC70-interacting protein knockout mouse model (FIG. 7A) and on biotin-avidin capture processed purified mitochondrial extracts from PND35 WT, Het, or CHIP KO mice (FIG 7C).
WB Mouse
Su YC, Chiu HW, Hung JC, Hong JR. Beta-nodavirus B2 protein induces hydrogen peroxide production, leading to Drp1-recruited mitochondrial fragmentation and cell death via mitochondrial targeting. Apoptosis 2014 Jul 10 [PMID: 25008790] (WB, Fish)

Details:
DRP1 antibody used for WB at 1:500 dilution on mitochondrial fractions isolated from Grouper/Epinephelus coioides cell line GF-1 cells (a cell line susceptible to grouper nervous necrosis virus - GNNV) transfected with EYFP or EYFP-B2 and treated with and without NAC or Mdivi for 48 h (Fig 6C).
WB Fish
Amadoro G, Corsetti V, Florenzano F et al. AD-linked, toxic NH2 human tau affects the quality control of mitochondria in neurons. Neurobiol. Dis. 2014 Feb 1 [PMID: 24411077] (WB, Rat) WB Rat
Kawada I, Hasina R, Lennon FE et al. Paxillin Mutations Affect Focal Adhesions and Lead to Altered Mitochondrial Dynamics: Relevance to Lung Cancer. Cancer Biol Ther 2013 May 31 [PMID: 23792636] (WB, IHC-P, Human) WB, IHC-P Human
Show All 16 Publications.

Reviews for DRP1 Antibody (NB110-55288) (2) 2.52

Average Rating: 2.5
(Based on 2 reviews)
We have 2 review tested in 1 species: Rat.
We have 2 review tested in 1 application: WB.

Reviews using NB110-55288:
Filter by Applications
WB
(1)
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Rat
(1)
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Images Ratings Applications Species Date Details
Western Blot DRP1 NB110-55288
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reviewed by:
Ning Wang
WB Rat 06/09/2014
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Summary

ApplicationWestern Blot
Sample TestedPC12 whole cell lysate
SpeciesRat
LotI can't find the original antibody vial.

Blocking

Blocking Details5% milk in PBST for 1 hour at room temperature

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionGoat anti rabbit IgG, HRP
Secondary Manufacturer Cat#Millipore #AP307P
Secondary Concentration1:2000

Details

Detection NotesECL
 
reviewed by:
12/31/1969
View

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DRP1 Antibody (NB110-55288) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-55288AF405
Alexa Fluor 488 Labeled NB110-55288AF488
Alexa Fluor 647 Labeled NB110-55288AF647
Alexa Fluor 700 Labeled NB110-55288AF700
Biotin Labeled NB110-55288B
DyLight 405 Labeled NB110-55288V
DyLight 488 Labeled NB110-55288G
DyLight 550 Labeled NB110-55288R
DyLight 650 Labeled NB110-55288C
FITC Labeled NB110-55288F
HRP Labeled NB110-55288H

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Blogs on DRP1.

Dynamin-related Protein 1 (DRP1) in Mitochondria and Apoptosis.
Dynamin-related Protein 1 (DRP1) is known to function in mitochondrial and peroxisomal division and mediate membrane fission through oligomerization into ring-like structure and sever the mitochondrial membrane, through a GTP hydrolysis-dependent mech...  Read full blog post.

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Recent Reviews

2.5
5
1
4
0
3
0
2
0
1
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1

Ning Wang
06/09/2014
Application: WB
Species: Rat

 

12/31/1969
Application:
Species:

Bioinformatics

Gene Symbol DNM1L
Entrez
Uniprot