DRP1 Antibody

Images

 
Western Blot: DRP1 Antibody [NB110-55288] - Total protein from human HeLa and MCF7 cells, mouse 3T3 cells and rat PC12 cells was separated on a 7.5 % gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% ...read more
Immunohistochemistry-Paraffin: DRP1 Antibody [NB110-55288] - Analysis of FFPE tissue section of mouse kidney using DRP1 antibody #NB110-55288 at 1:300. The primary antibody bound to DRP1 protein in the tissue section ...read more
Immunocytochemistry/ Immunofluorescence: DRP1 Antibody [NB110-55288] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were ...read more
Immunohistochemistry: DRP1 Antibody [NB110-55288] - Staining of renal tubular epithelium and visceral epithelial cells of the glomerulus. Human kidney cortex, 40X magnification.
Genetic Strategies: Western Blot: DRP1 Antibody [NB110-55288] - Lane 1: DRP1 knockout. Lane 2: DRP1 wildtype MEFs. Stained with NB110-55288 at 1:500.
Immunocytochemistry/ Immunofluorescence: DRP1 Antibody [NB110-55288] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were ...read more
Flow Cytometry: DRP1 Antibody [NB110-55288] - An intracellular stain was performed on HeLa cells with DRP1 Antibody NB110-55288AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Western Blot: DRP1 Antibody [NB110-55288] - Rat spinal cord-DRP1 (81kda). Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: DRP1 Antibody [NB110-55288] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: DRP1 Antibody [NB110-55288] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were ...read more
Flow Cytometry: DRP1 Antibody [NB110-55288] - An intracellular stain was performed on HeLa cells with NB110-55288 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. ...read more
Simple Western: DRP1 Antibody [NB110-55288] - Image shows a specific band for DUX4 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Summary
Reactivity Hu, Mu, Rt, Fi, Pm, BvSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

DRP1 Antibody Summary

Immunogen
A synthetic peptide made to an internal region within residues 500-600 of the human DRP1 protein. [Swiss-Prot# O00429]
Localization
Cytoplasm; cytosol. Intracytoplasmic membrane; peripheral mambrane protein. Mainly cytosolic. Also membrane-associated. Localizes to mitochondia at spots of division events. Associated with peroxisomal membranes, it is recruited in part by PEX11B.
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNM1L
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Simple Western 1:50
  • Flow Cytometry 1:1000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:300 - 1:1000
  • Immunohistochemistry-Paraffin 1:300 - 1:1000
  • Immunoprecipitation
Application Notes
In Western blot a band is seen at ~81 kDa. Immunoprecipitation was reported in scientific literature.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Kidney Whole Tissue Lysate (Adult Whole Normal)
DRP1 Overexpression Lysate
Control Peptide
DRP1 Peptide (NB110-55288PEP)
Reviewed Applications
Read 2 Reviews rated 5
using
NB110-55288 in the following applications:

Publications
Read Publications using
NB110-55288 in the following applications:

  • 4 publications
  • IHC
    2 publications
  • 1 publication
  • IP
    2 publications
  • WB
    16 publications

Reactivity Notes

Fish reactivity reported in scientific literature (PMID: 25008790)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DRP1 Antibody

  • DLP1
  • DRP1Dnm1p/Vps1p-like protein
  • DVLPEC 3.6.5.5
  • Dymple
  • DYMPLEDYNIV-11
  • dynamin 1-like
  • Dynamin family member proline-rich carboxyl-terminal domain less
  • dynamin-1-like protein
  • Dynamin-like protein 4
  • Dynamin-like protein IV
  • Dynamin-like protein
  • Dynamin-related protein 1
  • FLJ41912
  • HDYNIV
  • VPS1

Background

DRP1 (dynamin-related protein 1) is a highly conserved large GTPase protein which implicates in several important structural features of mitochondria, including mitochondrial shape, size, distribution, remodeling and maintenance. It functions in mitochondrial as well as peroxisomal division, and facilitates membrane fission via oligomerization into ring-like structures that wrap around fission site to constrict/split the membranes through a GTP hydrolysis-dependent mechanism. For normal brain development, DRP1 plays an essential role by mediating developmentally-regulated apoptosis during neural tube development. It is also required for a normal rate of cytochrome c release or caspase activation during apoptosis and for mitochondrial fission during mitosis, programmed necrosis execution and also for vesicle transport. Moreover, it is associated with mitochondrial structural and functional alterations such as fragmentation, phosphorylation, SUMOylation, ubiquitination and cell death. Drp1 interacts with amyloid beta, causing abnormal mitochondrial dynamics and neuronal damage in Alzheimer's disease patients, and defective DRP1 is also linked to encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-55288
Species: Hu, Mu, Rt, Fi, Pm, Bv
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP

Publications for DRP1 Antibody (NB110-55288)(26)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Fish.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ICC/IF
(4)
IHC
(2)
IHC-P
(1)
IP
(2)
WB
(16)
All Applications
Filter By Species
Human
(7)
Mouse
(11)
Rat
(3)
Fish
(1)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using NB110-55288 Applications Species
Abid H, Ryan ZC, Delmotte P et al. Extramyocellular interleukin-6 influences skeletal muscle mitochondrial physiology through canonical JAK/STAT signaling pathways FASEB J. Sep 3 2020 [PMID: 32885495]
Teodorof-Diedrich C, Spector SA Human Immunodeficiency Virus Type-1 and Methamphetamine Mediated Mitochondrial Damage and Neuronal Degeneration in Human Neurons J. Virol. Aug 12 2020 [PMID: 32796068]
Mesquita PHC, Lamb DA, Parry HA et al. Acute and chronic effects of resistance training on skeletal muscle markers of mitochondrial remodeling in older adults Physiol Rep Aug 1 2020 [PMID: 32748504] (WB, Human) WB Human
Maremanda K, Sundar I, Rahman I Protective role of mesenchymal stem cells and mesenchymal stem cell-derived exosomes in cigarette smoke-induced mitochondrial dysfunction in mice Toxicology and Applied Pharmacology 2019 Oct 01 [PMID: 31678243] (WB, Mouse) WB Mouse
Navas-Madronal M, Rodriguez C, Kassan M et al. Enhanced endoplasmic reticulum and mitochondrial stress in abdominal aortic aneurysm Clin. Sci. Jun 25 2019 [PMID: 31239294] (Human) Human
Zhong X, Cui P, Cai Y et al. Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells Cell Metab. Dec 1 2018 [PMID: 30527743] (WB, Mouse) WB Mouse
Pan JX, Tang F, Xiong F et al. APP promotes osteoblast survival and bone formation by regulating mitochondrial function and preventing oxidative stress. Cell Death Dis. Oct 22 2018 [PMID: 30349052] (WB, Mouse) WB Mouse
Tarpey MD, Davy KP, McMillan RP et al. Skeletal muscle autophagy and mitophagy in endurance-trained runners before and after a high-fat meal. Mol Metab. 2017 Oct 21 [PMID: 29097020] (Human) Human
Kandimalla R, Manczak M, Yin X et al. Hippocampal Phosphorylated Tau Induced Cognitive Decline, Dendritic Spine Loss and Mitochondrial Abnormalities in a Mouse Model of Alzheimer's Disease. Hum. Mol. Genet. 2017 Oct 12 [PMID: 29040533] (Mouse) Mouse
Vartanian V, Tumova J, Dobrzyn P et al. 8-oxoguanine DNA glycosylase (OGG1) deficiency elicits coordinated changes in lipid and mitochondrial metabolism in muscle PLoS ONE 2017 Jul 20 [PMID: 28727777] (WB, Mouse) WB Mouse
Show All 26 Publications.

Reviews for DRP1 Antibody (NB110-55288) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Rat.

Reviews using NB110-55288:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot DRP1 NB110-55288
Enlarge
5
reviewed by:
nitesh sharma
WB Human 10/05/2018
View

Summary

ApplicationWestern Blot
Sample TestedJurkat cell line
SpeciesHuman
LotD-2
Western Blot DRP1 NB110-55288
Enlarge
5
reviewed by:
Hyun Yi
WB Rat 04/25/2014
View

Summary

ApplicationWestern Blot
Sample TestedRat
SpeciesRat
LotC1

Blocking

Blocking DetailsRapid block, 1x, 1hr, room temperature

Primary Anitbody

Dilution Ratio1:2000, overnight, 4d.c, 2% BSA

Secondary Antibody

Secondary Descriptionnormal rabbit IgG-HRP
Secondary Manufacturer Cat#sc-2749
Secondary Concentration1:2000

Details

Detection Notesuse BioRad chemidox, SuperSignal West Dura Chemiluminescent Substrate

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DRP1 Antibody (NB110-55288) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-55288AF350
Alexa Fluor 405 NB110-55288AF405
Alexa Fluor 488 NB110-55288AF488
Alexa Fluor 532 NB110-55288AF532
Alexa Fluor 594 NB110-55288AF594
Alexa Fluor 647 NB110-55288AF647
Alexa Fluor 700 NB110-55288AF700
Alexa Fluor 750 NB110-55288AF750
Biotin NB110-55288B
DyLight 350 NB110-55288UV
DyLight 405 NB110-55288V
DyLight 488 NB110-55288G
DyLight 550 NB110-55288R
DyLight 594 NB110-55288DL594
DyLight 650 NB110-55288C
DyLight 680 NB110-55288FR
DyLight 755 NB110-55288IR
FITC NB110-55288F
HRP NB110-55288H
Janelia Fluor 549 NB110-55288JF549
Janelia Fluor 646 NB110-55288JF646

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Bioinformatics Tool for DRP1 Antibody (NB110-55288)

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Blogs on DRP1.

Understanding Mitophagy Mechanisms: Canonical PINK1/Parkin, LC3-Dependent Piecemeal, and LC3-Independent Mitochondrial Derived Vesicles
By Christina Towers, PhD What is Mitophagy?The selective degradation of mitochondria via double membrane autophagosome vesicles is called mitophagy. Damaged mitochondria can generate harmful amounts of reactive ox...  Read full blog post.


  Read full blog post.

Dynamin-related Protein 1 (DRP1) in Mitochondria and Apoptosis.
Dynamin-related Protein 1 (DRP1) is known to function in mitochondrial and peroxisomal division and mediate membrane fission through oligomerization into ring-like structure and sever the mitochondrial membrane, through a GTP hydrolysis-dependent mech...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

nitesh sharma
10/05/2018
Application: WB
Species: Human

Hyun Yi
04/25/2014
Application: WB
Species: Rat

Bioinformatics

Gene Symbol DNM1L
Entrez
Uniprot