DRP1 Antibody


Western Blot: DRP1 Antibody [NB110-55288] - Total protein from human HeLa and MCF7 cells, mouse 3T3 cells and rat PC12 cells was separated on a 7.5 % gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% ...read more
Immunohistochemistry-Paraffin: DRP1 Antibody [NB110-55288] - IHC analysis of a formain fixed paraffin embedded (FFPE) tissue section of mouse kidney using DRP1 antibody #NB110-55288 at 1: 300 dilution. The primary ...read more
Immunohistochemistry: DRP1 Antibody [NB110-55288] - Staining of renal tubular epithelium and visceral epithelial cells of the glomerulus. Human kidney cortex, 40X magnification.
Genetic Strategies: Western Blot: DRP1 Antibody [NB110-55288] - Lane 1: DRP1 knockout Lane 2: DRP1 wildtype MEFs Stained with NB110-55288 at 1:500x dilution.
Immunocytochemistry/ Immunofluorescence: DRP1 Antibody [NB110-55288] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were ...read more
Flow Cytometry: DRP1 Antibody [NB110-55288] - An intracellular stain was performed on HeLa cells with DRP1 Antibody NB110-55288 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% ...read more
Western Blot: DRP1 Antibody [NB110-55288] - Rat spinal cord-DRP1 (81kda). Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: DRP1 Antibody [NB110-55288] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Simple Western: DRP1 Antibody [NB110-55288] - Simple Western lane view shows a specific band for DUX4 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Reactivity Hu, Mu, Rt, Fi, Pm, BvSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP
1 mg/ml
Validated by:

Genetic Strategies


DRP1 Antibody Summary

A synthetic peptide made to an internal region within residues 500-600 of the human DRP1 protein. [Swiss-Prot# O00429]
Cytoplasm; cytosol. Intracytoplasmic membrane; peripheral mambrane protein. Mainly cytosolic. Also membrane-associated. Localizes to mitochondia at spots of division events. Associated with peroxisomal membranes, it is recruited in part by PEX11B. May als
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:500
  • Simple Western 1:50
  • Flow Cytometry 1:1000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:300-1:1000
  • Immunohistochemistry-Paraffin 1:300-1:1000
  • Immunoprecipitation
Application Notes
This DRP1 antibody is useful for Immonohistochemistry paraffin embedded sections, Immunocytochemistry/Immunofluorescence and Western blot analysis where a band is seen at approx. 81 kDa. In ICC/IF, cytoplasmic staining was observed in HeLa cells. Immunoprecipitation was reported in scientific literature.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Kidney Lysate (NB820-59231)
DRP1 Lysate (NBL1-09966)
Control Peptide
DRP1 Peptide (NB110-55288PEP)
Reviewed Applications
Read 1 Review rated 5
NB110-55288 in the following applications:

Read Publications using
NB110-55288 in the following applications:

  • 4 publications
  • IHC
    2 publications
  • 1 publication
  • IP
    2 publications
  • WB
    11 publications

Reactivity Notes

Human, primate, rat, mouse. Predicted to react with bovine based on 100% sequence homology. Fish reactivity reported in scientific literature (PMID: 25008790)

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS and 0.02% azide
0.05% Sodium Azide
1 mg/ml
Immunogen affinity purified

Alternate Names for DRP1 Antibody

  • DLP1
  • DRP1Dnm1p/Vps1p-like protein
  • Dymple
  • dynamin 1-like
  • Dynamin family member proline-rich carboxyl-terminal domain less
  • dynamin-1-like protein
  • Dynamin-like protein 4
  • Dynamin-like protein IV
  • Dynamin-like protein
  • Dynamin-related protein 1
  • FLJ41912
  • VPS1


DRP1 (dynamin-related protein 1) is a highly conserved large GTPase protein which implicates in several important structural features of mitochondria, including mitochondrial shape, size, distribution, remodeling and maintenance. It functions in mitochondrial as well as peroxisomal division, and facilitates membrane fission via oligomerization into ring-like structures that wrap around fission site to constrict/split the membranes through a GTP hydrolysis-dependent mechanism. For normal brain development, DRP1 plays an essential role by mediating developmentally-regulated apoptosis during neural tube development. It is also required for a normal rate of cytochrome c release or caspase activation during apoptosis and for mitochondrial fission during mitosis, programmed necrosis execution and also for vesicle transport. Moreover, it is associated with mitochondrial structural and functional alterations such as fragmentation, phosphorylation, SUMOylation, ubiquitination and cell death. Drp1 interacts with amyloid beta, causing abnormal mitochondrial dynamics and neuronal damage in Alzheimer's disease patients, and defective DRP1 is also linked to encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Bv, Mk
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Species: Hu, Mu
Applications: WB, IHC, IHC-Fr
Species: Hu, Mu
Applications: WB, Simple Western, ELISA, Flow, IHC, IHC-Fr, IHC-P
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Rb
Applications: WB, ICC/IF, IHC, IHC-P, IP, PEP-ELISA
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-Fr, IHC-P, IP
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Ca
Applications: WB, IHC, IHC-P, IP
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt, Fi, Pm, Bv
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP

Publications for DRP1 Antibody (NB110-55288)(18)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Fish.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB110-55288 Applications Species
Tarpey MD, Davy KP, McMillan RP et al. Skeletal muscle autophagy and mitophagy in endurance-trained runners before and after a high-fat meal. Mol Metab. 2017 Oct 21 [PMID: 29097020] (Human) Human
Kandimalla R, Manczak M, Yin X et al. Hippocampal Phosphorylated Tau Induced Cognitive Decline, Dendritic Spine Loss and Mitochondrial Abnormalities in a Mouse Model of Alzheimer's Disease. Hum. Mol. Genet. 2017 Oct 12 [PMID: 29040533] (Mouse) Mouse
Wang L, Zhang T, Wang L et al. Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission. EMBO J. Apr 4 2017 [PMID: 28377463] (WB, Mouse) WB Mouse
Chan YL, Saad S, Al-Odat I et al. Maternal L-Carnitine Supplementation Improves Brain Health in Offspring from Cigarette Smoke Exposed Mothers. Front Mol Neurosci. 2017 Feb 28 [PMID: 28243190] (WB, Mouse) WB Mouse
Xu S, Wang P, Zhang H et al. CaMKII induces permeability transition through Drp1 phosphorylation during chronic B-AR stimulation. Nat Commun Oct 14 2016 [PMID: 27739424] (IP, WB, Rat) IP, WB Rat
Chen H, Chan YL, Nguyen LT et al. Moderate traumatic brain injury is linked to acute behaviour deficits and long term mitochondrial alterations. Clin. Exp. Pharmacol. Physiol. Aug 25 2016 [PMID: 27557565] (IHC, Rat) IHC Rat
Lennon FE, Cianci GC, Kanteti R et al. Unique fractal evaluation and therapeutic implications of mitochondrial morphology in malignant mesothelioma. Sci Rep. 2016 Apr 15 [PMID: 27080907] (WB, Human)

DRP1 antibody was used for WB analysis of total cell lysates as well as mitochondrial fractions from MeT-5A (control transformed non-tumorigenic mesothelial cells), and mesothelioma cell lines namely H28, H513, H2052, H2461 (Epithelioid) and H2373, H2596
WB Human
Zhang M, Sun T, Jian C et al. Remodeling of Mitochondrial Flashes in Muscular Development and Dystrophy in Zebrafish. PLoS One 2015 [PMID: 26186000] (ICC/IF) ICC/IF
Palubinsky AM, Stankowski JN, Kale AC et al. CHIP is an Essential Determinant of Neuronal Mitochondrial Stress Signaling Antioxid. Redox Signal. 2015 Jan 20 [PMID: 25602369] (WB, Mouse)

DRP1 antibody was used for WB application on whole brain lysates from PND35 - CHIP/C-terminus of HSC70-interacting protein knockout mouse model (FIG. 7A) and on biotin-avidin capture processed purified mitochondrial extracts from PND35 WT, Het, or CHIP KO mice (FIG 7C).
WB Mouse
Su YC, Chiu HW, Hung JC, Hong JR. Beta-nodavirus B2 protein induces hydrogen peroxide production, leading to Drp1-recruited mitochondrial fragmentation and cell death via mitochondrial targeting. Apoptosis 2014 Jul 10 [PMID: 25008790] (WB, Fish)

DRP1 antibody used for WB at 1:500 dilution on mitochondrial fractions isolated from Grouper/Epinephelus coioides cell line GF-1 cells (a cell line susceptible to grouper nervous necrosis virus - GNNV) transfected with EYFP or EYFP-B2 and treated with and without NAC or Mdivi for 48 h (Fig 6C).
WB Fish
Show All 18 Publications.

Review for DRP1 Antibody (NB110-55288) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB110-55288:
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Images Ratings Applications Species Date Details
Western Blot DRP1 NB110-55288
reviewed by:
Hyun Yi
WB Rat 04/25/2014


ApplicationWestern Blot
Sample TestedRat


Blocking DetailsRapid block, 1x, 1hr, room temperature

Primary Anitbody

Dilution Ratio1:2000, overnight, 4d.c, 2% BSA

Secondary Antibody

Secondary Descriptionnormal rabbit IgG-HRP
Secondary Manufacturer Cat#sc-2749
Secondary Concentration1:2000


Detection Notesuse BioRad chemidox, SuperSignal West Dura Chemiluminescent Substrate

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DRP1 Antibody (NB110-55288) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-55288AF405
Alexa Fluor 488 Labeled NB110-55288AF488
Alexa Fluor 647 Labeled NB110-55288AF647
Alexa Fluor 700 Labeled NB110-55288AF700
Biotin Labeled NB110-55288B
DyLight 405 Labeled NB110-55288V
DyLight 488 Labeled NB110-55288G
DyLight 550 Labeled NB110-55288R
DyLight 650 Labeled NB110-55288C
FITC Labeled NB110-55288F
HRP Labeled NB110-55288H

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Blogs on DRP1.

Dynamin-related Protein 1 (DRP1) in Mitochondria and Apoptosis.
Dynamin-related Protein 1 (DRP1) is known to function in mitochondrial and peroxisomal division and mediate membrane fission through oligomerization into ring-like structure and sever the mitochondrial membrane, through a GTP hydrolysis-dependent mech...  Read full blog post.

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Recent Reviews


Hyun Yi
Application: WB
Species: Rat


Gene Symbol DNM1L