DAP Kinase 2 Antibody (OTI1C5)

Images

 
Western Blot: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-DAP Kinase 2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; ...read more
Immunocytochemistry/ Immunofluorescence: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DAP Kinase 2.
Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Human pancreas tissue using anti-DAP Kinase 2 mouse monoclonal antibody.
Flow Cytometry: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DAP Kinase 2 antibody, ...read more
Western Blot: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP Kinase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts ...read more
Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DAP Kinase 2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DAP Kinase 2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Human colon tissue using anti-DAP Kinase 2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Human Kidney tissue using anti-DAP Kinase 2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Human liver tissue using anti-DAP Kinase 2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Human lung tissue using anti-DAP Kinase 2 mouse monoclonal antibody.
Flow Cytometry: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Analysis of Jurkat cells, using anti-DAP Kinase 2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Immunoprecipitation: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - (Negative control: IP without adding anti-DAPK2 antibody.). For each experiment, 500ul of DDK tagged DAPK2 overexpression lysates (at 1:5 dilution with ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clone
OTI1C5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.76 mg/ml

Order Details

DAP Kinase 2 Antibody (OTI1C5) Summary

Immunogen
Full length human recombinant protein of human DAPK2 (NP_055141) produced in HEK293T cell.
Isotype
IgG3
Clonality
Monoclonal
Host
Mouse
Gene
DAPK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 2ug/500ul
  • Western Blot 1:1000-2000
Theoretical MW
42.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.76 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DAP Kinase 2 Antibody (OTI1C5)

  • DAP Kinase 2
  • DAPK2
  • DAP-kinase-related protein 1 beta isoform
  • DAP-kinase-related protein 1
  • death-associated protein kinase 2
  • DRP1
  • DRP-1
  • DRP-1MGC119312
  • EC 2.7.11
  • EC 2.7.11.1

Background

This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DAPK2
Entrez