DAP Kinase 2 Antibody (OTI1C5)

Images

 
Western Blot: DAP Kinase 2 Antibody (1C5) [NBP2-02477] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-DAP Kinase 2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; ...read more
Immunocytochemistry/ Immunofluorescence: DAP Kinase 2 Antibody (1C5) [NBP2-02477] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DAP Kinase 2.
Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (1C5) [NBP2-02477] - Staining of paraffin-embedded Human pancreas tissue using anti-DAP Kinase 2 mouse monoclonal antibody.
Flow Cytometry: DAP Kinase 2 Antibody (1C5) [NBP2-02477] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DAP Kinase 2 antibody, and ...read more
Western Blot: DAP Kinase 2 Antibody (1C5) [NBP2-02477] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP Kinase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (1C5) [NBP2-02477] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DAP Kinase 2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (1C5) [NBP2-02477] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DAP Kinase 2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (1C5) [NBP2-02477] - Staining of paraffin-embedded Human colon tissue using anti-DAP Kinase 2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (1C5) [NBP2-02477] - Staining of paraffin-embedded Human Kidney tissue using anti-DAP Kinase 2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (1C5) [NBP2-02477] - Staining of paraffin-embedded Human liver tissue using anti-DAP Kinase 2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (1C5) [NBP2-02477] - Staining of paraffin-embedded Human lung tissue using anti-DAP Kinase 2 mouse monoclonal antibody.
Flow Cytometry: DAP Kinase 2 Antibody (1C5) [NBP2-02477] - Analysis of Jurkat cells, using anti-DAP Kinase 2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Rt, Ca, MkSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
OTI1C5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

DAP Kinase 2 Antibody (OTI1C5) Summary

Immunogen
Full length human recombinant protein of human DAPK2 (NP_055141) produced in HEK293T cell.
Isotype
IgG3
Clonality
Monoclonal
Host
Mouse
Gene
DAPK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 2ug/500ul
Theoretical MW
42.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DAP Kinase 2 Antibody (OTI1C5)

  • DAP kinase 2
  • DAP-kinase-related protein 1 beta isoform
  • DAP-kinase-related protein 1
  • death-associated protein kinase 2
  • DRP1
  • DRP-1MGC119312
  • EC 2.7.11
  • EC 2.7.11.1

Background

This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DAP Kinase 2 Antibody (NBP2-02477) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-71753AF350
Alexa Fluor 405 NBP2-71753AF405
Alexa Fluor 488 NBP2-71753AF488
Alexa Fluor 532 NBP2-71753AF532
Alexa Fluor 594 NBP2-71753AF594
Alexa Fluor 647 NBP2-71753AF647
Alexa Fluor 700 NBP2-71753AF700
Alexa Fluor 750 NBP2-71753AF750
Allophycocyanin NBP2-71753APC
Allophycocyanin/Cy7 NBP2-71753APCCY7
Biotin NBP2-71753B
DyLight 350 NBP2-71753UV
DyLight 405 NBP2-71753V
DyLight 488 NBP2-71753G
DyLight 550 NBP2-71753R
DyLight 594 NBP2-71753DL594
DyLight 650 NBP2-71753C
DyLight 680 NBP2-71753FR
DyLight 755 NBP2-71753IR
FITC NBP2-71753F
HRP NBP2-71753H
Janelia Fluor 549 NBP2-71753JF549
Janelia Fluor 646 NBP2-71753JF646
PE NBP2-71753PE
PE/Atto594 NBP2-71753PEATT594
PE/Cy5.5 NBP2-71753PECY55
PE/Cy7 NBP2-71753PECY7
PerCP NBP2-71753PCP

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Bioinformatics

Gene Symbol DAPK2
Entrez