LRRK2 Antibody Summary
A C-terminal synthetic peptide made to the human LRRK2 protein sequence (between residues 2500-2527). [UniProt# Q5S007]
Mitochondrial outer membrane.
Immunogen affinity purified
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- Western Blot 1:2000 - 1:5000
- Flow Cytometry 1:200
- Immunocytochemistry/Immunofluorescence 1:1000 - 1:2000
- Immunohistochemistry 1:500 - 1:1000
- Immunohistochemistry-Paraffin 1:500 - 1:1000
- Immunoprecipitation 1:10 - 1:500
In Western blot, a band can be seen at ~286 kDa. We have also seen other bands with some lysates, but these bands have been blocked by the control peptide, suggesting that these bands are degradation products. IP has been done in an LRRK2 autophosphorylation kinase assay, IHC has been done on brain sections and ICC/IF has been done on transfected cell lines. Frozen sections using the LRRK2 antibody were from a customer review. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
|Read Publications using
NB300-268 in the following applications:
Reacts with Drosophila and moth. Immunogen sequence has 87% homology to rat.
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.1% Sodium Azide
Immunogen affinity purified
Alternate Names for LRRK2 Antibody
- augmented in rheumatoid arthritis 17
- EC 18.104.22.168
- leucine-rich repeat kinase 2
- leucine-rich repeat serine/threonine-protein kinase 2
- Parkinson disease (autosomal dominant) 8
LRRK2 (leucine-rich repeat serine/threonine-protein kinase 2, also called PARK8 or Dardarin) is a homodimeric multifunctional protein that belongs to TKL serine threonine protein kinases family and LRRK2 immunoreactivity has been documented in neurofibrillary tangles in Alzheimer's disease as well as in PDCG (parkinsonism-dementia complex of Guam). Highly expressed in tissues such as liver, heart, brain etc., LRRK2 localizes to cellular cytoplasm and also associates with membrane structures including mitochondrial outer membrane. LRRK interacts with PARK2, PRDX3 and TPCN2, and phosphorylate proteins which are implicated in Parkinson disease. LRRK2 is a cytoplasmic kinase with autophosphorylation capability as well as GTPase activity. Its interaction with microtubules suggests that LRRK2-induced neurodegeneration might be partly mediated via inhibition of microtubule dynamics, and LRRK2 -mitochondria interactiosn suggests its implication in pathways eliciting ROS mediated damage. LRRK2 has been shown exert positive regulation in autophagy via calcium-sensitive activation of CaMKK/AMPK signaling pathway through activation of NAADP receptors, increase of lysosomal pH as well as calcium release.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Product General Protocols
View specific protocols for LRRK2 Antibody (NB300-268):
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for LRRK2 Antibody (NB300-268). (Showing 1 - 2 of 2 FAQs).
I am wondering what concentration of Lrrk2 blocking peptide (NB300-268PEP) I should use for FACS as a control?
- We do have a protocol for how to incubate the primary with the blocking peptide to perform a negative control competition assay. While this was written with WB and IHC in mind, it should work the same in inhibiting cell binding for flow.
Is the immunogen of NB300-268 within amino acids 922 and 1143 of LRRK2 (Uniprot ID#Q5S007)?
- The immunogen used to generate NB300-268 was a synthetic peptide made to a C-terminal region within residues 2500-2527 of the human LRRK2 protein (UniProt# Q5S007). We do have an alternative antibody that you may be interested in, with catalog number NB110-55289. The immunogen used to generate this product was synthetic peptide made to an internal region of the mouse LRRK2 protein sequence (between residues 900-1000), although this does not recognize human LRRK2. If you are looking to detect mouse LRRK2 this would be a suitable product for you.
Other Available Formats
Additional Array Products
Bioinformatics Tool for LRRK2 Antibody (NB300-268)
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Blogs on LRRK2.