LRRK2 Antibody - BSA Free

Images

 
Independent Antibodies: Western Blot: LRRK2 Antibody [NB300-268] - Western blotting of LRRK2. Recombinant LRRK2 (arrowhead) from transfected (+) HEK 293T and M17 cells was specifically recognized by all four ...read more
Immunohistochemistry-Frozen: LRRK2 Antibody [NB300-268] - Staining as described in PMID 24312256. Image from verified customer review.
Genetic Strategies: Knockout Validated: LRRK2 Antibody [NB300-268] - Mn increases LRRK2 kinase activity and expression in RAW 264.7 and HMC3 cells. Two-hundred (200) ug of protein were collected from cell ...read more
Western Blot: LRRK2 Antibody [NB300-268] - LRRK2 N-terminal sequences show increased aggregation. Western blots of cell lysates of SH-SY5Y cells transfected with indicated LRRK2 construct showed equal expression of the ...read more
Immunohistochemistry-Paraffin: LRRK2 Antibody [NB300-268] - Specificity of immunostaining for LRRK2. Specificity of the antibodies used for immunocytochemistry was determined by performing adsorptions with the ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: LRRK2 Antibody [NB300-268] - Immunocytochemistry of LRRK2 in PD using antibodies (Ab1, Ab4) raised against sequences corresponding to various regions shown ...read more
Flow Cytometry: LRRK2 Antibody [NB300-268] - Analysis using the Alexa Fluor 647 conjugate of NB300-268. Staining of LRRK2 in normal human peripheral blood cells (anticoagulated Lithium-Heparin) using anti-LRRK2 antibody ...read more
Immunohistochemistry-Paraffin: LRRK2 Antibody [NB300-268] - LRRK2 accumulates in globules in alphaS tg mice. Triple immunofluorescence for alphaS, LRRK2 and Rab5B for basal ganglia in alphaS tg mice. LRRK2 and Rab5B ...read more
Immunocytochemistry/ Immunofluorescence: LRRK2 Antibody [NB300-268] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
Flow Cytometry: LRRK2 Antibody [NB300-268] - An intracellular stain was performed on U2OS cells with LRRK2 Antibody NB300-268 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Immunocytochemistry/ Immunofluorescence: LRRK2 Antibody [NB300-268] - LRRK2 antibody was tested in SH-SY-5Y cells at a 1:200 dilution against DyLight 488 (Green). Alpha tubulin and nuclei were counterstained against ...read more
Immunohistochemistry-Paraffin: LRRK2 Antibody [NB300-268] - Staining of human brain, Putamen, Neurons and Glia using a 60X magnification.
Flow Cytometry: LRRK2 Antibody [NB300-268] - An intracellular stain was performed on U2OS cells with LRRK Antibody NB300-268AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Flow Cytometry: LRRK2 Antibody [NB300-268] - An intracellular stain was performed on Neuro2a cells with LRRK2 Antibody NB300-268 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
LRRK2 kinase inhibition blocks Tat-induced S935 phosphorylation and inflammatory cytokine expression in BV-2 cells. (A) Western blot depicting pS935-LRRK2 and total LRRK2 protein levels 12 hours after saline or ...read more
The interaction and phosphorylation of LRRK2 and LC1. (A) Cellular co-localisation of endogenous LRRK2 and MAP1B (LC1). SKNSH cells were labelled with LRRK2 (green) and LC1 (red) antibodies and signals were detected ...read more
Metformin sensitized hypoxic tumor cells to sorafenib. a Hypoxic tumor cells were resistant to sorafenib compared to cells under normoxia. MHCC97H cells were treated with 400 μM CoCl2 for 24 h, then incubated with ...read more
APE1 is acetylated after binding to AP sites in the chromatin. (A) BJ-hTERT cells were treated with MX (50 mM) for the indicated times. IF was performed using anti-APE1 and anti-AcAPE1, and counterstaining with DAPI was ...read more
EXO1 influences DNA repair and DNA replication in Nbs1 mutants. (A) Schematic illustration of the SA-GFP based SSA assay. (B) Western blotting of cells transfected with or without a vector expressing human NBS1. The ...read more
BRD4770-induced activation of the FA pathway does not occur via the direct induction of DNA damage or altered cell cycle progression(A) BJ-TERT cells were incubated with (+) and without (-) 200 nM mitomycin C (MMC) in ...read more
MiR-181a overexpression impairs and miR-181a inhibition improves glucose and lipid metabolism in vitro(A, B, C, D and E) Hepatocytes were transfected with 10 nM miR-181a mimics or negative controls in the absence or ...read more
Myocardial infarction (MI) increased TLR3 expression and its physical association with Trif in mouse myocardium. Heart samples were taken from infarct area at 4 weeks after MI. (A) and (B) show mRNA and protein levels ...read more
Effects of MPP Versus PHTPP on NE Regulation of VMN Nitrergic and GABA Neuron Adrenergic Receptor Protein Expression. Pooled lysates of laser-microdissected VMN nNOS- or GAD-immunopositive neurons from groups of female ...read more
Downregulation of La autoantigen, hVAP-A, PSMA7, and NS5B leads to reduction in viral replication: overnight grown Huh-7.5 cells were transfected with La autoantigen, hVAP-A, and PSMA7 siRNAs for 48 h. Cells were then ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, Ce, I, PlSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

LRRK2 Antibody - BSA Free Summary

Immunogen
A C-terminal synthetic peptide made to the human LRRK2 protein sequence (between residues 2500-2527). [UniProt# Q5S007]
Localization
Mitochondrial outer membrane.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LRRK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular) 2.5 ug/ml
  • Flow Cytometry 2.5 ug/ml
  • Immunocytochemistry/ Immunofluorescence 2 - 5 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • Knockout Validated
  • Western Blot 1 - 2 ug/ml
Application Notes
In Western blot, a band can be seen at ~286 kDa. We have also seen other bands with some lysates, but these bands have been blocked by the control peptide, suggesting that these bands are degradation products. IP has been done in an LRRK2 autophosphorylation kinase assay, IHC has been done on brain sections and ICC/IF has been done on transfected cell lines. Frozen sections using the LRRK2 antibody were from a customer review. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
286 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
LRRK2 Peptide (NB300-268PEP)
Reviewed Applications
Read 1 Review rated 5
using
NB300-268 in the following applications:

Publications
Read Publications using
NB300-268 in the following applications:

Reactivity Notes

Human, Moth reactivity reported in scientific literature (PMID: 19824698). Plant reactivity reported in scientific literature (PMID: 27273569).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LRRK2 Antibody - BSA Free

  • augmented in rheumatoid arthritis 17
  • AURA17
  • Dardarin
  • DKFZp434H2111
  • EC 2.7.11.1
  • FLJ45829
  • leucine-rich repeat kinase 2
  • leucine-rich repeat serine/threonine-protein kinase 2
  • LRRK2
  • PARK8
  • PARK8DARDARIN
  • Parkinson disease (autosomal dominant) 8
  • RIPK7
  • ROCO2

Background

LRRK2 (leucine-rich repeat serine/threonine-protein kinase 2, also called PARK8 or Dardarin) is a homodimeric multifunctional protein that belongs to TKL serine threonine protein kinases family and LRRK2 immunoreactivity has been documented in neurofibrillary tangles in Alzheimer's disease as well as in PDCG (parkinsonism-dementia complex of Guam). Highly expressed in tissues such as liver, heart, brain etc., LRRK2 localizes to cellular cytoplasm and also associates with membrane structures including mitochondrial outer membrane. LRRK interacts with PARK2, PRDX3 and TPCN2, and phosphorylate proteins which are implicated in Parkinson disease. LRRK2 is a cytoplasmic kinase with autophosphorylation capability as well as GTPase activity. Its interaction with microtubules suggests that LRRK2-induced neurodegeneration might be partly mediated via inhibition of microtubule dynamics, and LRRK2 -mitochondria interactiosn suggests its implication in pathways eliciting ROS mediated damage. LRRK2 has been shown exert positive regulation in autophagy via calcium-sensitive activation of CaMKK/AMPK signaling pathway through activation of NAADP receptors, increase of lysosomal pH as well as calcium release.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LRRK2 Antibody (NB300-268)(46)

We have publications tested in 3 confirmed species: Human, Mouse, C. elegans.

We have publications tested in 8 applications: FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
FLOW
(2)
ICC/IF
(9)
IF/IHC
(4)
IHC
(1)
IHC-Fr
(1)
IHC-P
(2)
IP
(2)
WB
(18)
All Applications
Filter By Species
Human
(29)
Mouse
(11)
C. elegans
(2)
All Species
Showing Publications 1 - 10 of 46. Show All 46 Publications.
Publications using NB300-268 Applications Species
Pajarillo E, Kim SH, Digman A et al. The role of microglial LRRK2 kinase in manganese-induced inflammatory neurotoxicity via NLRP3 inflammasome and RAB10-mediated autophagy dysfunction The Journal of biological chemistry 2023-06-01 [PMID: 37269951] (Mouse) Mouse
Pajarillo E, Kim SH, Digman A et al. The role of microglial LRRK2 in manganese-induced inflammatory neurotoxicity via NLRP3 inflammasome and RAB10-mediated autophagy dysfunction bioRxiv : the preprint server for biology 2023-04-05 [PMID: 37066140] (IHC, Mouse) IHC Mouse
Wang H, Li J, Tao L et al. MiR-205 Regulates LRRK2 Expression in Dopamine Neurons in Parkinson's Disease through Methylation Modification Iranian Journal of Public Health 2022-07-17 [PMID: 36248294]
Wallings RL, Hughes LP, Staley HA Et al. WHOPPA Enables Parallel Assessment of Leucine-Rich Repeat Kinase 2 and Glucocerebrosidase Enzymatic Activity in Parkinson's Disease Monocytes Front Cell Neurosci 2022-06-27 [PMID: 35755775] (FLOW, Human)

Details:
Citation using the Alexa Fluor 700 version of this antibody.
FLOW Human
Bharat V, Hsieh CH, Wang X Mitochondrial Defects in Fibroblasts of Pathogenic MAPT Patients Frontiers in cell and developmental biology 2021-11-03 [PMID: 34805172] (WB, Human) WB Human
Chen S, Luo Z, Ward C et al. Generation of two LRRK2 homozygous knockout human induced pluripotent stem cell lines using CRISPR/Cas9 Stem Cell Res 2020-04-21 [PMID: 32339904] (WB, Human) WB Human
Kim J, Pajarillo E, Rizor A et al. LRRK2 kinase plays a critical role in manganese-induced inflammation and apoptosis in microglia. PLoS ONE 2019-01-15 [PMID: 30645642] (WB, Mouse) WB Mouse
Bonello F, Hassoun SM, Mouton-Liger F et al. LRRK2 impairs PINK1/Parkin-dependent mitophagy via its kinase activity: pathologic insights into Parkinson's disease. Hum. Mol. Genet. 2019-01-09 [PMID: 30629163] (ICC/IF, Human) ICC/IF Human
Takanashi M, Funayama M, Matsuura E et al. Isolated nigral degeneration without pathological protein aggregation in autopsied brains with LRRk2 p.R1441H homozygous and heterozygous mutations. Acta Neuropathol Commun. 2018-10-17 [PMID: 30333048] (IHC-P, Human) IHC-P Human
Lim J, Bang Y, Choi JH et al. LRRK2 G2019S Induces Anxiety/Depression-like Behavior before the Onset of Motor Dysfunction with 5-HT1A Receptor Upregulation in Mice J Neurosci. 2018-02-13 [PMID: 29305532] (IHC-P, Mouse) IHC-P Mouse
Show All 46 Publications.

Review for LRRK2 Antibody (NB300-268) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB300-268:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen LRRK2 NB300-268
Enlarge
5
reviewed by:
Masahiro Nakamori
IHC-Fr Human 01/28/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LRRK2 Antibody (NB300-268). (Showing 1 - 2 of 2 FAQs).

  1. I am wondering what concentration of Lrrk2 blocking peptide (NB300-268PEP) I should use for FACS as a control?
    • We do have a protocol for how to incubate the primary with the blocking peptide to perform a negative control competition assay. While this was written with WB and IHC in mind, it should work the same in inhibiting cell binding for flow.
  2. Is the immunogen of NB300-268 within amino acids 922 and 1143 of LRRK2 (Uniprot ID#Q5S007)?
    • The immunogen used to generate NB300-268 was a synthetic peptide made to a C-terminal region within residues 2500-2527 of the human LRRK2 protein (UniProt# Q5S007). We do have an alternative antibody that you may be interested in, with catalog number NB110-55289. The immunogen used to generate this product was synthetic peptide made to an internal region of the mouse LRRK2 protein sequence (between residues 900-1000), although this does not recognize human LRRK2. If you are looking to detect mouse LRRK2 this would be a suitable product for you.

Secondary Antibodies

 

Isotype Controls

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Research Areas for LRRK2 Antibody (NB300-268)

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Blogs on LRRK2.

The role of Parkin and autophagy in retinal pigment epithelial cell (RPE) degradation
The root of Parkinson’s disease (PD) points to a poorly regulated electron transport chain leading to mitochondrial damage, where many proteins need to work cohesively to ensure proper function.  The two key players of this pathway are PINK1, ...  Read full blog post.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Parkinson's Disease Infographic
Parkinson's disease affects the nervous system which controls movement. Damage to the levels of dopamine in the brain impairs the ability to relay messages to parts of the body which control movement. While the exact cause of the disease is unknown, r...  Read full blog post.

Novus Antibodies Highlighted in Parkinson's Disease Research
Identified almost two centuries ago, Parkinson's disease is a neurodegenerative disorder that afflicts an estimated 4-6 million worldwide (www.parkinsons.org). The prevalence of Parkinson's disease is expected to grow considerably as the average age o...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
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Masahiro Nakamori
01/28/2014
Application: IHC-Fr
Species: Human

Bioinformatics

Gene Symbol LRRK2
Entrez
Uniprot