LRRK2 Antibody

Images

 
Western Blot: LRRK2 Antibody [NB300-268] - Detection of LRRK2 in 50 ug of crude bovine brain membrane.
Immunocytochemistry/ Immunofluorescence: LRRK2 Antibody [NB300-268] - Mouse CAD cells transfected with Human wild-type LRRK-2 (1:2,000).
Immunohistochemistry-Paraffin: LRRK2 Antibody [NB300-268] - Staining of human brain, Putamen, Neurons and Glia using a 60x magnification.
Flow Cytometry: LRRK2 Antibody [NB300-268] - Analysis using the Alexa Fluor (R) 647 conjugate of NB300-268. Staining of LRRK2 in normal human peripheral blood cells (anticoagulated Lithium-Heparin) using anti-LRRK2 ...read more
Western Blot: LRRK2 Antibody [NB300-268] - Detection of LRRK2 in HeLa whole cell lysate (RIPA) using NB300-268. 1:5000 dilution, 1 minute ECL detection.
Immunocytochemistry/ Immunofluorescence: LRRK2 Antibody [NB300-268] - LRRK2 antibody was tested in SH-SY-5Y cells at a 1:200 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against ...read more
Immunohistochemistry-Frozen: LRRK2 Antibody [NB300-268] - Staining as described in PMID 24312256. Image from confirmed customer review.
Immunoprecipitation: LRRK2 Antibody [NB300-268] - Immunoprecipitation of LRRK2 from SH-SY5Y cell lysate followed by an autophosphorylation assay using NB300-268. Lane 1 Rabbit IgG, Lanes 2 and 3 NB300-268.

Product Details

Summary
Reactivity Hu, Mu, Bv, CeSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

LRRK2 Antibody Summary

Immunogen
A C-terminal synthetic peptide made to the human LRRK2 protein sequence (between residues 2500-2527). [UniProt# Q5S007]
Localization
Mitochondrial outer membrane.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LRRK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:5000
  • Flow Cytometry 1:200
  • Immunocytochemistry/Immunofluorescence 1:1000-1:2000
  • Immunohistochemistry 1:500-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Immunoprecipitation 1:10-1:500
Application Notes
In Western blot. a band can be seen at ~286 kDa. We have also seen other bands with some lysates, but these bands have been blocked by the control peptide, suggesting that these bands are degradation products. IP has been done in an LRRK2 autophosphorylation kinase assay, IHC has been done on brain sections and ICC/IF has been done on transfected cell lines. Frozen sections using the LRRK2 antibody were from a customer review. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
286 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
LRRK2 Lysate (NBP2-66407)
Control Peptide
LRRK2 Peptide (NB300-268PEP)
Reviewed Applications
Read 1 Review rated 5
using
NB300-268 in the following applications:

Publications
Read Publications using
NB300-268 in the following applications:

  • 1 publication
  • 7 publications
  • IHC
    4 publications
  • 1 publication
  • IP
    1 publication
  • WB
    15 publications

Reactivity Notes

Drosophila and moth. Immunogen sequence has 87% homology to rat.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LRRK2 Antibody

  • augmented in rheumatoid arthritis 17
  • AURA17
  • Dardarin
  • DKFZp434H2111
  • EC 2.7.11.1
  • FLJ45829
  • leucine-rich repeat kinase 2
  • leucine-rich repeat serine/threonine-protein kinase 2
  • LRRK2
  • PARK8
  • PARK8DARDARIN
  • Parkinson disease (autosomal dominant) 8
  • RIPK7
  • ROCO2

Background

LRRK2 (leucine-rich repeat serine/threonine-protein kinase 2, also called PARK8 or Dardarin) is a homodimeric multifunctional protein that belongs to TKL serine threonine protein kinases family and LRRK2 immunoreactivity has been documented in neurofibrillary tangles in Alzheimer's disease as well as in PDCG (parkinsonism-dementia complex of Guam). Highly expressed in tissues such as liver, heart, brain etc., LRRK2 localizes to cellular cytoplasm and also associates with membrane structures including mitochondrial outer membrane. LRRK interacts with PARK2, PRDX3 and TPCN2, and phosphorylate proteins which are implicated in Parkinson disease. LRRK2 is a cytoplasmic kinase with autophosphorylation capability as well as GTPase activity. Its interaction with microtubules suggests that LRRK2-induced neurodegeneration might be partly mediated via inhibition of microtubule dynamics, and LRRK2 -mitochondria interactiosn suggests its implication in pathways eliciting ROS mediated damage. LRRK2 has been shown exert positive regulation in autophagy via calcium-sensitive activation of CaMKK/AMPK signaling pathway through activation of NAADP receptors, increase of lysosomal pH as well as calcium release.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for LRRK2 Antibody (NB300-268)(33)

We have publications tested in 4 confirmed species: Human, Mouse, C. elegans, Moth.

We have publications tested in 6 applications: FLOW, ICC/IF, IHC, IHC-Fr, IP, WB.


Filter By Application
FLOW
(1)
ICC/IF
(7)
IHC
(4)
IHC-Fr
(1)
IP
(1)
WB
(15)
All Applications
Filter By Species
Human
(23)
Mouse
(5)
C. elegans
(2)
Moth
(1)
All Species
Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using NB300-268 Applications Species
Bliederhaeuser C, Zondler L, Grozdanov V et al. LRRK2 contributes to monocyte dysregulation in Parkinson's disease. Acta Neuropathol Commun. Nov 24 2016 [PMID: 27884177] (FLOW, Human) FLOW Human
Nucifora FC, Nucifora LG, Ng CH et al. Ubiqutination via K27 and K29 chains signals aggregation and neuronal protection of LRRK2 by WSB1. Nat Commun Jun 9 2016 [PMID: 27273569]
Chan SL, Chua LL, Angeles DC et al. MAP1B rescues LRRK2 mutant-mediated cytotoxicity. Mol Brain 2014 Apr 25 [PMID: 24754922] (ICC/IF, WB, Human) ICC/IF, WB Human
Angeles DC, Ho P, Chua LL et al. Thiol peroxidases ameliorate LRRK2 mutant-induced mitochondrial and dopaminergic neuronal degeneration in Drosophila. Hum. Mol. Genet. 2014 Feb 27 [PMID: 24459295] (WB, Human) WB Human
Kamikawaji S, Ito G, Sano T, Iwatsubo T. Differential Effects of Familial Parkinson Mutations in LRRK2 Revealed by a Systematic Analysis of Autophosphorylation. Biochemistry. 2013 Aug 23 [PMID: 23924436] (WB, Human) WB Human
Marker DF, Puccini JM, Mockus TE et al. LRRK2 kinase inhibition prevents pathological microglial phagocytosis in response to HIV-1 Tat protein J Neuroinflammation 2012 Nov 29 [PMID: 23190742] (ICC/IF, Mouse) ICC/IF Mouse
Pandey N, Fahey MT, Jong YJ, O'Malley KL. Sequences Located within the N-Terminus of the PD-Linked LRRK2 Lead to Increased Aggregation and Attenuation of 6-Hydroxydopamine-Induced Cell Death PLoS One 2012 [PMID: 23028814] (WB, Human) WB Human
Sekigawa A, Fujita M, Sekiyama K et al. Distinct mechanisms of axonal globule formation in mice expressing human wild type alpha-synuclein or dementia with Lewy bodies-linked P123H beta-synuclein. Mol Brain. 2012 Sep 26 [PMID: 23013868] (IHC-Fr, Mouse) IHC-Fr Mouse
Ito G, Iwatsubo T. Re-examination of the dimerization state of leucine-rich repeat kinase 2: predominance of the monomeric form. Biochem J. 2011 Nov 2. [PMID: 22047502]
Liu Z, Lee J, Krummey S et al. The kinase LRRK2 is a regulator of the transcription factor NFAT that modulates the severity of inflammatory bowel disease. Nat Immunol;12(11):1063-1070. 2011 [PMID: 21983832]
Show All 33 Publications.

Review for LRRK2 Antibody (NB300-268) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB300-268:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen LRRK2 NB300-268
Enlarge
reviewed by:
Masahiro Nakamori
IHC-Fr Human 01/28/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
SpeciesHuman
Pub Med ID24312256
FileView PDF

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LRRK2 Antibody (NB300-268). (Showing 1 - 2 of 2 FAQs).

  1. I am wondering what concentration of Lrrk2 blocking peptide (NB300-268PEP) I should use for FACS as a control?
    • We do have a protocol for how to incubate the primary with the blocking peptide to perform a negative control competition assay. While this was written with WB and IHC in mind, it should work the same in inhibiting cell binding for flow.
  2. Is the immunogen of NB300-268 within amino acids 922 and 1143 of LRRK2 (Uniprot ID#Q5S007)?
    • The immunogen used to generate NB300-268 was a synthetic peptide made to a C-terminal region within residues 2500-2527 of the human LRRK2 protein (UniProt# Q5S007). We do have an alternative antibody that you may be interested in, with catalog number NB110-55289. The immunogen used to generate this product was synthetic peptide made to an internal region of the mouse LRRK2 protein sequence (between residues 900-1000), although this does not recognize human LRRK2. If you are looking to detect mouse LRRK2 this would be a suitable product for you.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB300-268AF405
Alexa Fluor 488 Labeled NB300-268AF488
Alexa Fluor 647 Labeled NB300-268AF647
Alexa Fluor 700 Labeled NB300-268AF700
Biotin Labeled NB300-268B
DyLight 405 Labeled NB300-268V
DyLight 488 Labeled NB300-268G
DyLight 550 Labeled NB300-268R
DyLight 650 Labeled NB300-268C
FITC Labeled NB300-268F
HRP Labeled NB300-268H

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Blogs on LRRK2.

The role of Parkin and autophagy in retinal pigment epithelial cell (RPE) degradation
The root of Parkinson’s disease (PD) points to a poorly regulated electron transport chain leading to mitochondrial damage, where many proteins need to work cohesively to ensure proper function.  The two key players of this pathway are PINK1,...  Read full blog post.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Parkinson's Disease Infographic
Parkinson's disease affects the nervous system which controls movement. Damage to the levels of dopamine in the brain impairs the ability to relay messages to parts of the body which control movement. While the exact cause of the disease is unknown, r...  Read full blog post.

Novus Antibodies Highlighted in Parkinson's Disease Research
Identified almost two centuries ago, Parkinson's disease is a neurodegenerative disorder that afflicts an estimated 4-6 million worldwide (www.parkinsons.org). The prevalence of Parkinson's disease is expected to grow considerably as the average age o...  Read full blog post.

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Recent Reviews

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Masahiro Nakamori
01/28/2014
Application: IHC-Fr
Species: Human

Bioinformatics

Gene Symbol LRRK2
Entrez
Uniprot