LRRK2 Antibody

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Independent Antibodies: Western Blot: LRRK2 Antibody [NB300-268] - Western blotting of LRRK2. Recombinant LRRK2 (arrowhead) from transfected (+) HEK 293T and M17 cells was specifically recognized by all four ...read more
Immunohistochemistry-Frozen: LRRK2 Antibody [NB300-268] - Staining as described in PMID 24312256. Image from verified customer review.
Genetic Strategies: Knockout Validated: LRRK2 Antibody [NB300-268] - Mn increases LRRK2 kinase activity and expression in RAW 264.7 and HMC3 cells. Two-hundred (200) ug of protein were collected from cell ...read more
Western Blot: LRRK2 Antibody [NB300-268] - LRRK2 N-terminal sequences show increased aggregation. Western blots of cell lysates of SH-SY5Y cells transfected with indicated LRRK2 construct showed equal expression of the ...read more
Immunohistochemistry-Paraffin: LRRK2 Antibody [NB300-268] - Specificity of immunostaining for LRRK2. Specificity of the antibodies used for immunocytochemistry was determined by performing adsorptions with the ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: LRRK2 Antibody [NB300-268] - Immunocytochemistry of LRRK2 in PD using antibodies (Ab1, Ab4) raised against sequences corresponding to various regions shown ...read more
Flow Cytometry: LRRK2 Antibody [NB300-268] - Analysis using the Alexa Fluor 647 conjugate of NB300-268. Staining of LRRK2 in normal human peripheral blood cells (anticoagulated Lithium-Heparin) using anti-LRRK2 antibody ...read more
Immunohistochemistry-Paraffin: LRRK2 Antibody [NB300-268] - LRRK2 accumulates in globules in alphaS tg mice. Triple immunofluorescence for alphaS, LRRK2 and Rab5B for basal ganglia in alphaS tg mice. LRRK2 and Rab5B ...read more
Immunocytochemistry/ Immunofluorescence: LRRK2 Antibody [NB300-268] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
Flow Cytometry: LRRK2 Antibody [NB300-268] - An intracellular stain was performed on U2OS cells with LRRK2 Antibody NB300-268 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Immunocytochemistry/ Immunofluorescence: LRRK2 Antibody [NB300-268] - LRRK2 antibody was tested in SH-SY-5Y cells at a 1:200 dilution against DyLight 488 (Green). Alpha tubulin and nuclei were counterstained against ...read more
Immunohistochemistry-Paraffin: LRRK2 Antibody [NB300-268] - Staining of human brain, Putamen, Neurons and Glia using a 60X magnification.
Flow Cytometry: LRRK2 Antibody [NB300-268] - An intracellular stain was performed on U2OS cells with LRRK Antibody NB300-268AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Flow Cytometry: LRRK2 Antibody [NB300-268] - An intracellular stain was performed on Neuro2a cells with LRRK2 Antibody NB300-268 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, Ce, I, PlSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

LRRK2 Antibody Summary

Immunogen
A C-terminal synthetic peptide made to the human LRRK2 protein sequence (between residues 2500-2527). [UniProt# Q5S007]
Localization
Mitochondrial outer membrane.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LRRK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/ml
  • Flow Cytometry 2.5 ug/ml
  • Immunocytochemistry/Immunofluorescence 2 - 5 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • Flow (Intracellular) 2.5 ug/ml
  • Knockout Validated
Application Notes
In Western blot, a band can be seen at ~286 kDa. We have also seen other bands with some lysates, but these bands have been blocked by the control peptide, suggesting that these bands are degradation products. IP has been done in an LRRK2 autophosphorylation kinase assay, IHC has been done on brain sections and ICC/IF has been done on transfected cell lines. Frozen sections using the LRRK2 antibody were from a customer review. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
286 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
LRRK2 Knockout HeLa Cell Lysate
Control Peptide
LRRK2 Peptide (NB300-268PEP)
Reviewed Applications
Read 1 Review rated 5
using
NB300-268 in the following applications:

Publications
Read Publications using
NB300-268 in the following applications:

Reactivity Notes

Human, Moth reactivity reported in scientific literature (PMID: 19824698). Plant reactivity reported in scientific literature (PMID: 27273569).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LRRK2 Antibody

  • augmented in rheumatoid arthritis 17
  • AURA17
  • Dardarin
  • DKFZp434H2111
  • EC 2.7.11.1
  • FLJ45829
  • leucine-rich repeat kinase 2
  • leucine-rich repeat serine/threonine-protein kinase 2
  • LRRK2
  • PARK8
  • PARK8DARDARIN
  • Parkinson disease (autosomal dominant) 8
  • RIPK7
  • ROCO2

Background

LRRK2 (leucine-rich repeat serine/threonine-protein kinase 2, also called PARK8 or Dardarin) is a homodimeric multifunctional protein that belongs to TKL serine threonine protein kinases family and LRRK2 immunoreactivity has been documented in neurofibrillary tangles in Alzheimer's disease as well as in PDCG (parkinsonism-dementia complex of Guam). Highly expressed in tissues such as liver, heart, brain etc., LRRK2 localizes to cellular cytoplasm and also associates with membrane structures including mitochondrial outer membrane. LRRK interacts with PARK2, PRDX3 and TPCN2, and phosphorylate proteins which are implicated in Parkinson disease. LRRK2 is a cytoplasmic kinase with autophosphorylation capability as well as GTPase activity. Its interaction with microtubules suggests that LRRK2-induced neurodegeneration might be partly mediated via inhibition of microtubule dynamics, and LRRK2 -mitochondria interactiosn suggests its implication in pathways eliciting ROS mediated damage. LRRK2 has been shown exert positive regulation in autophagy via calcium-sensitive activation of CaMKK/AMPK signaling pathway through activation of NAADP receptors, increase of lysosomal pH as well as calcium release.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LRRK2 Antibody (NB300-268)(39)

We have publications tested in 4 confirmed species: Human, Mouse, C. elegans, Moth.

We have publications tested in 7 applications: FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
FLOW
(1)
ICC/IF
(8)
IHC
(4)
IHC-Fr
(1)
IHC-P
(2)
IP
(1)
WB
(17)
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Filter By Species
Human
(26)
Mouse
(7)
C. elegans
(2)
Moth
(1)
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Showing Publications 1 - 10 of 39. Show All 39 Publications.
Publications using NB300-268 Applications Species
Chen S, Luo Z, Ward C et al. Generation of two LRRK2 homozygous knockout human induced pluripotent stem cell lines using CRISPR/Cas9 Stem Cell Res Apr 21 2020 [PMID: 32339904] (WB, Human) WB Human
Kim J, Pajarillo E, Rizor A et al. LRRK2 kinase plays a critical role in manganese-induced inflammation and apoptosis in microglia. PLoS ONE Jan 15 2019 [PMID: 30645642] (WB, Mouse) WB Mouse
Bonello F, Hassoun SM, Mouton-Liger F et al. LRRK2 impairs PINK1/Parkin-dependent mitophagy via its kinase activity: pathologic insights into Parkinson's disease. Hum. Mol. Genet. Jan 9 2019 [PMID: 30629163] (ICC/IF, Human) ICC/IF Human
Takanashi M, Funayama M, Matsuura E et al. Isolated nigral degeneration without pathological protein aggregation in autopsied brains with LRRk2 p.R1441H homozygous and heterozygous mutations. Acta Neuropathol Commun. Oct 17 2018 [PMID: 30333048] (IHC-P, Human) IHC-P Human
Lim J, Bang Y, Choi JH et al. LRRK2 G2019S Induces Anxiety/Depression-like Behavior before the Onset of Motor Dysfunction with 5-HT1A Receptor Upregulation in Mice J Neurosci. 2018 Feb 13 [PMID: 29305532] (IHC-P, Mouse) IHC-P Mouse
Bliederhaeuser C, Zondler L, Grozdanov V et al. LRRK2 contributes to monocyte dysregulation in Parkinson's disease. Acta Neuropathol Commun. Nov 24 2016 [PMID: 27884177] (FLOW, Human) FLOW Human
Nucifora FC, Nucifora LG, Ng CH et al. Ubiqutination via K27 and K29 chains signals aggregation and neuronal protection of LRRK2 by WSB1. Nat Commun Jun 9 2016 [PMID: 27273569]
Chan SL, Chua LL, Angeles DC et al. MAP1B rescues LRRK2 mutant-mediated cytotoxicity. Mol Brain 2014 Apr 25 [PMID: 24754922] (ICC/IF, WB, Human) ICC/IF, WB Human
Angeles DC, Ho P, Chua LL et al. Thiol peroxidases ameliorate LRRK2 mutant-induced mitochondrial and dopaminergic neuronal degeneration in Drosophila. Hum. Mol. Genet. 2014 Feb 27 [PMID: 24459295] (WB, Human) WB Human
Kamikawaji S, Ito G, Sano T, Iwatsubo T. Differential Effects of Familial Parkinson Mutations in LRRK2 Revealed by a Systematic Analysis of Autophosphorylation. Biochemistry. 2013 Aug 23 [PMID: 23924436] (WB, Human) WB Human
Show All 39 Publications.

Review for LRRK2 Antibody (NB300-268) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB300-268:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen LRRK2 NB300-268
Enlarge
5
reviewed by:
Masahiro Nakamori
IHC-Fr Human 01/28/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
SpeciesHuman
Pub Med ID24312256
FileView PDF

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LRRK2 Antibody (NB300-268). (Showing 1 - 2 of 2 FAQs).

  1. I am wondering what concentration of Lrrk2 blocking peptide (NB300-268PEP) I should use for FACS as a control?
    • We do have a protocol for how to incubate the primary with the blocking peptide to perform a negative control competition assay. While this was written with WB and IHC in mind, it should work the same in inhibiting cell binding for flow.
  2. Is the immunogen of NB300-268 within amino acids 922 and 1143 of LRRK2 (Uniprot ID#Q5S007)?
    • The immunogen used to generate NB300-268 was a synthetic peptide made to a C-terminal region within residues 2500-2527 of the human LRRK2 protein (UniProt# Q5S007). We do have an alternative antibody that you may be interested in, with catalog number NB110-55289. The immunogen used to generate this product was synthetic peptide made to an internal region of the mouse LRRK2 protein sequence (between residues 900-1000), although this does not recognize human LRRK2. If you are looking to detect mouse LRRK2 this would be a suitable product for you.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB300-268AF350
Alexa Fluor 405 NB300-268AF405
Alexa Fluor 488 NB300-268AF488
Alexa Fluor 532 NB300-268AF532
Alexa Fluor 594 NB300-268AF594
Alexa Fluor 647 NB300-268AF647
Alexa Fluor 700 NB300-268AF700
Alexa Fluor 750 NB300-268AF750
Allophycocyanin NB300-268APC
Biotin NB300-268B
DyLight 350 NB300-268UV
DyLight 405 NB300-268V
DyLight 488 NB300-268G
DyLight 550 NB300-268R
DyLight 594 NB300-268DL594
DyLight 650 NB300-268C
DyLight 680 NB300-268FR
DyLight 755 NB300-268IR
FITC NB300-268F
HRP NB300-268H
Janelia Fluor 549 NB300-268JF549
Janelia Fluor 646 NB300-268JF646
PE NB300-268PE
PerCP NB300-268PCP

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Blogs on LRRK2.

The role of Parkin and autophagy in retinal pigment epithelial cell (RPE) degradation
The root of Parkinson’s disease (PD) points to a poorly regulated electron transport chain leading to mitochondrial damage, where many proteins need to work cohesively to ensure proper function.  The two key players of this pathway are PINK1,...  Read full blog post.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Parkinson's Disease Infographic
Parkinson's disease affects the nervous system which controls movement. Damage to the levels of dopamine in the brain impairs the ability to relay messages to parts of the body which control movement. While the exact cause of the disease is unknown, r...  Read full blog post.

Novus Antibodies Highlighted in Parkinson's Disease Research
Identified almost two centuries ago, Parkinson's disease is a neurodegenerative disorder that afflicts an estimated 4-6 million worldwide (www.parkinsons.org). The prevalence of Parkinson's disease is expected to grow considerably as the average age o...  Read full blog post.

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Recent Reviews

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5
1
4
0
3
0
2
0
1
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Masahiro Nakamori
01/28/2014
Application: IHC-Fr
Species: Human

Bioinformatics

Gene Symbol LRRK2
Entrez
Uniprot