HTRA2/Omi Antibody [Unconjugated]

Images

 
Western blot shows lysates of PC‑12 rat adrenal pheochromocytoma cell line, Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, L‑929 mouse fibroblast cell line, and ...read more
HTRA2/Omi was detected in immersion fixed Jurkat human acute T cell leukemia cell line stimulated with staurosporin using Rabbit Anti-Human/Mouse/Rat HTRA2/Omi Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for HTRA2/Omi at approximately 51 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

HTRA2/Omi Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human HTRA2/Omi
Ala134-Glu458
Accession # O43464
Specificity
Detects human, mouse, and rat full length and mitochondria-processed HTRA2/Omi.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HTRA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Simple Western 2.5 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 4
using
AF1458 in the following applications:

Publications
Read Publications using
AF1458 in the following applications:

  • ICC
    5 publications
  • IF
    1 publication
  • WB
    14 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HTRA2/Omi Antibody [Unconjugated]

  • High temperature requirement protein A2
  • HtrA serine peptidase 2
  • HTRA2
  • HtrA-like serine protease
  • Omi
  • OMIOmi stress-regulated endoprotease
  • PARK13EC 3.4.21.108
  • PRSS25
  • PRSS25serine, 25
  • Serine protease 25
  • serine protease HTRA2, mitochondrial
  • Serine proteinase OMI

Background

HtrA2/Omi is the mammalian homologue of bacterial high temperature requirement protein (HtrA). HtrA2/Omi localizes to the mitochondria and is processed to expose an amino-terminal Reaper-like motif similar to SMAC/Diablo. HtrA2/Omi is released from the mitochondria in response to apoptotic insult and can interact with the BIR2 or BIR3 domains of XIAP to relieve caspase-IAP inhibition. This effect can be measured by reversing XIAP-BIR2 (R&D Systems, Catalog # 786-XB) inhibition of Caspase-7 (R&D Systems, Catalog # 823-C7) cleavage of a fluorogenic peptide (DEVD-AFC, MP Bio, Catalog # AFC-138). IC50 values for this effect are typically between 0.2 and 1.5 μM. HtrA2/Omi is trimeric and functions as a serine protease. The serine protease activity may play a more central role in apoptosis than its IAP antagonizing function. A PDZ domain regulates the serine protease activity by blocking access to the active site. The specificity of the protease is yet to be defined and no endogenous substrates are known to date.

  1. Suzuki, Y. et al. (2001) Mol. Cell. 8:613.
  2. van Loo, G. et al. (2002) Cell Death & Diff. 9:20.
  3. Hedge, R. et al. (2001) J. Biol. Chem. 277:432.
  4. Verhagen, A. et al. (2001) J. Biol. Chem. 277:445.
  5. Martins, L. et al. (2002) J. Biol. Chem. 277:439.
  6. Silke, J., and A. Verhagen (2002) Cell Death & Diff. 9:362.
  7. Savopoulos, J. et al. (2000) Protein Expression & Purification 19:227.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HTRA2/Omi Antibody (AF1458)(21)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Hamster.

We have publications tested in 3 applications: ICC, IF, WB.


Filter By Application
ICC
(5)
IF
(1)
WB
(14)
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Human
(12)
Mouse
(5)
Rat
(2)
Hamster
(1)
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Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using AF1458 Applications Species
S Saita, H Nolte, KU Fiedler, H Kashkar, AS Venne, RP Zahedi, M Krüger, T Langer PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis Nat. Cell Biol, 2017;0(0):. 2017 [PMID: 28288130] (WB,, Human) WB Human
Wu J, Li M, Cao L, Sun M, Chen D, Ren H, Xia Q, Tao Z, Qin Z, Hu Q, Wang G Protease Omi cleaving Hax-1 protein contributes to OGD/R-induced mitochondrial damage in neuroblastoma N2a cells and cerebral injury in MCAO mice. Acta Pharmacol Sin, 2015;36(9):1043-52. 2015 [PMID: 26299953] (WB,, Mouse) WB Mouse
Ma Q, Hu Q, Xu R, Zhen X, Wang G Protease Omi facilitates neurite outgrowth in mouse neuroblastoma N2a cells by cleaving transcription factor E2F1. Acta Pharmacol Sin, 2015;36(8):966-75. 2015 [PMID: 26238290] (WB,, Human) WB Human
Morey T, Roufayel R, Johnston D, Fletcher A, Mosser D Heat shock inhibition of CDK5 increases NOXA levels through miR-23a repression. J Biol Chem, 2015;290(18):11443-54. 2015 [PMID: 25829494] (WB,, Human) WB Human
Patterson, Victoria, Zullo, Alfred J, Koenig, Claire, Stoessel, Sean, Jo, Hakryul, Liu, Xinran, Han, Jinah, Choi, Murim, DeWan, Andrew T, Thomas, Jean-Leo, Kuan, Chia-Yi, Hoh, Josephin Neural-specific deletion of Htra2 causes cerebellar neurodegeneration and defective processing of mitochondrial OPA1. PLoS ONE, 2014;9(12):e115789. 2014 [PMID: 25531304] (WB,, Mouse) WB Mouse
Aerts L, Craessaerts K, de Strooper B, Morais V PINK1 kinase catalytic activity is regulated by phosphorylation on serines 228 and 402. J Biol Chem, 2015;290(5):2798-811. 2015 [PMID: 25527497] (WB,, Human) WB Human
Yoshizumi T, Ichinohe T, Sasaki O, Otera H, Kawabata S, Mihara K, Koshiba T Influenza A virus protein PB1-F2 translocates into mitochondria via Tom40 channels and impairs innate immunity. Nat Commun, 2014;5(0):4713. 2014 [PMID: 25140902] (WB,, Human) WB Human
Geisler S, Vollmer S, Golombek S, Kahle P The ubiquitin-conjugating enzymes UBE2N, UBE2L3 and UBE2D2/3 are essential for Parkin-dependent mitophagy. J Cell Sci, 2014;127(0):3280-93. 2014 [PMID: 24906799]
Yamauchi S, Hou Y, Guo A, Hirata H, Nakajima W, Yip A, Yu C, Harada I, Chiam K, Sawada Y, Tanaka N, Kawauchi K p53-mediated activation of the mitochondrial protease HtrA2/Omi prevents cell invasion. J Cell Biol, 2014;204(7):1191-207. 2014 [PMID: 24662565] (IF,, Mouse) IF Mouse
Jin, Seok Min, Youle, Richard The accumulation of misfolded proteins in the mitochondrial matrix is sensed by PINK1 to induce PARK2/Parkin-mediated mitophagy of polarized mitochondria. Autophagy, 2013;9(11):1750-7. 2013 [PMID: 24149988] (WB,, Human) WB Human
Show All 21 Publications.

Review for HTRA2/Omi Antibody (AF1458) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1458:
Filter by Applications
IF
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Human 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23183826
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23183826

Details

Detection NotesSee PMID 23183826

Comments

CommentsPublished See PMID 23183826

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Anonymous
01/05/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol HTRA2
Entrez
Uniprot