Western Blot: PINK1 Antibody [NB100-644] - Analysis of C-terminally V5His-tagged human PINK1 or N-teminally myc-tagged human PINK1 expressed in HEK293T cells using NB100-644 at 1:1000 dilution.
Simple Western: PINK1 Antibody [NB100-644] - Simple Western lane view shows a specific band for PINK1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Western blot - Use at 1:500 to 1:1,000 dilution. This antibody has only been tested on transfected lysates. Endogenous protein detection is unknown. No other applications have been tested.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Mutations in the PTEN-induced kinase (PINK1) gene located within the PARK6 locus on chromosome 1p35-p36 have recently been identified in patients with recessive early-onset Parkinson disease. Data suggests that PINK1 may be the second most common causative gene next to parkin in parkinsonism with the recessive mode of inheritance.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
PINK1: All work and no fun The protein PINK1 is a mitochondrial-located serine/threonine kinase (PTK) that maintains organelle function and integrity. It not only protects organelles from cellular stress, but it also uses the selective auto-phagocytosis process for cleaning and... Read full blog post.
PINK1 and its role in Parkinson's disease PINK1 (PTEN induced putative kinase 1) is a mitochondrial serine/threonine kinase which maintains mitochondrial function/integrity, provides protection against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondr... Read full blog post.
PINK1: A Critical Player in Mitophagy PINK1 (PTEN-induced putative kinase 1) is a mitochondrial directed serine-threonine kinase, that regulates normal mitochondrial function and transport vital to normal performance of neurons and neuronal survival. PINK1 has been shown to be localized t... Read full blog post.