Periostin/OSF-2 Antibody

Images

 
Western Blot: Periostin/OSF-2 Antibody [NBP1-30042] - Rat lung lysate showing specific immunolabeling of the ~ 93 kDa periostin protein doublet.
Immunohistochemistry: Periostin/OSF-2 Antibody [NBP1-30042] - Analysis of periostin in rabbit periosteum. Image courtesy of product review submitted by Samer Zaky.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Periostin/OSF-2 Antibody Summary

Immunogen
Bacterial fusion protein equivalent to a 188-amino acid polypeptide from the C-terminal region of mouse periostin which is comprised of six small alternatively-spliced exons.
Specificity
Specific for the ~93 kDa periostin doublet in mouse lung extract. Consistent with the fact that this antibody is made against an alternatively spliced region of periostin, this antibody recognizes only the largest two of the three forms of periostin recognized on Western blots by the Pan periostin antibody and also shows a distinctive staining pattern by immunohistochemistry. The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 degrees C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POSTN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
Application Notes
For IHC-P antigen retrieval protocol (incubate slides for 20 minutes at 90 degrees C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Immunocytochemistry/Immunofluorescence was reported in scientific literature.
Theoretical MW
93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4
using
NBP1-30042 in the following applications:

Publications
Read Publications using
NBP1-30042 in the following applications:

Reactivity Notes

Expected reactivity to all mammalian and avian species. Based on customer review rabbit reacts.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Periostin/OSF-2 Antibody

  • Fasciclin I-like
  • MGC119510
  • MGC119511
  • OSF2
  • OSF-2
  • OSF-2osteoblast specific factor 2 (fasciclin I-like)
  • OSF2periodontal ligament-specific periostin
  • Osteoblast-specific factor 2
  • PDLPOSTN
  • periostin isoform thy2
  • periostin isoform thy4
  • periostin isoform thy6
  • periostin isoform thy8
  • Periostin
  • periostin, osteoblast specific factor
  • PNRP11-412K4.1
  • POSTN
  • TRIF52

Background

Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblastspecific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Ch, Rb
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Periostin/OSF-2 Antibody (NBP1-30042)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC
(2)
IHC-P
(2)
WB
(7)
All Applications
Filter By Species
Human
(1)
Mouse
(8)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP1-30042 Applications Species
Schwanekamp JA, Lorts A, Sargent MA et al. TGFBI functions similar to periostin but is uniquely dispensable during cardiac injury PLoS ONE 2017 Jul 27 [PMID: 28750100] (WB, Mouse) WB Mouse
Paglioni Md, Silva WG, Pereira J et al. Intravenous Bisphosphonates Therapy does not Thicken Cementum or Change Periodontal Ligament of Cancer Patients. Oral Surg Oral Med Oral Pathol Oral Radiol. Feb 6 2017 [PMID: 28407986] (IHC, Human) IHC Human
Liu Y, Zhang P, Pan J et al. Anatomical differences of the protein profile in the rabbit sclera during growth. Exp. Eye Res. Nov 5 2016 [PMID: 27826074] (WB, Rabbit) WB Rabbit
Vanhoutte D, Schips TG, Kwong JQ et al. Thrombospondin expression in myofibers stabilizes muscle membranes Elife Sep 26 2016 [PMID: 27669143] (IHC, Mouse) IHC Mouse
Kanisicak O, Khalil H, Ivey MJ et al. Genetic lineage tracing defines myofibroblast origin and function in the injured heart Nat Commun 2016 Jul 24 [PMID: 27447449] (WB) WB
Murphy S, Dowling P, Zweyer M et al. Proteomic analysis of dystrophin deficiency and associated changes in the aged mdx-4cv heart model of dystrophinopathy-related cardiomyopathy J Proteomics Mar 4 2016 [PMID: 26961938] (WB, Mouse) WB Mouse
Murphy S, Henry M, Meleady P et al. Simultaneous Pathoproteomic Evaluation of the Dystrophin-Glycoprotein Complex and Secondary Changes in the mdx-4cv Mouse Model of Duchenne Muscular Dystrophy Biology (Basel). 2015 Jun 13 [PMID: 26067837] (WB, Mouse) WB Mouse
Holland A, Dowling P, Meleady P et al. Label-free mass spectrometric analysis of the mdx-4cv diaphragm identifies the matricellular protein periostin as a potential factor involved in dystrophinopathy-related fibrosis Proteomics 2015 Mar 03 [PMID: 25737063] (WB, Mouse)

Details:
Periostin antibody used for WB/immunoblot analysis of periostin protein in the diaphragm of mdx-4cv, mdx and WT mice. In this paper, this antibody was tested in parallal to another antibody from different supplier, and NBP1-30042 generated very intense and specific signal compared to the other antibody (Figure 4B and 4F, Figure 5).
WB Mouse
Nolte Zadeh-Khorasani M, Safarov O et al. Oxazolone and ethanol induce colitis in non-obese diabetic-severe combined immunodeficiency interleukin-2RGamma(null) mice engrafted with human peripheral blood mononuclear cells Clin Exp Immunol. 2013 May [PMID: 23574330] (IHC-P, Mouse) IHC-P Mouse
Casanova JC, Travisano S, de la Pompa JL. Epithelial-to-mesenchymal transition in epicardium is independent of snail1. Genesis 2013 Jan [PMID: 23097346] (IHC-P, ICC/IF, Mouse) IHC-P, ICC/IF Mouse
Show All 11 Publications.

Reviews for Periostin/OSF-2 Antibody (NBP1-30042) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Other.
We have 2 reviews tested in 2 application: IHC-P, IHC.

Reviews using NBP1-30042:
Filter by Applications
IHC-P
(1)
IHC
(1)
All Applications
Filter by Species
Mouse
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Mouse 12/23/2014
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 23574330
SpeciesMouse
Special ApplicationsPublished in PMID 23574330
Immunohistochemistry Periostin/OSF-2 NBP1-30042
Enlarge
reviewed by:
Anonymous
IHC Other 03/15/2011
View

Summary

ApplicationImmunohistochemistry
Sample TestedRabbit periosteum
SpeciesOther
LotSH310R
CommentsThe antibody works pretty well for rabbit periosteum in paraffin IHC. I performed tripsin Ag retrieval.

Comments

CommentsThe antibody works pretty well for rabbit periosteum in paraffin IHC. I performed tripsin Ag retrieval.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Periostin/OSF-2 Antibody (NBP1-30042) (0)

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

 
Anonymous
12/23/2014
Application: IHC-P
Species: Mouse

Anonymous
03/15/2011
Application: IHC
Species: Other

Bioinformatics

Gene Symbol POSTN
Entrez
Uniprot