Tenascin C Antibody (4C8MS)

Images

 
Flow (Intracellular): Tenascin C Antibody (4C8MS) [NB110-68136] - Figure A: An intracellular stain was performed on U87MG Cells with Tenascin C (4C8MS) antibody NB110-68136 (blue) and a matched isotype control ...read more
Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [NB110-68136] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone-tendon using Tenascin C antibody (clone 4C8MS) at 1:25 dilution. ...read more
Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [NB110-68136] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone-tendon using Tenascin C antibody (clone 4C8MS) at 1:25 dilution. ...read more
Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Tenascin C antibody (dark blue). Isotype control shown in orange. An antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
Clone
4C8MS
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Tenascin C Antibody (4C8MS) Summary

Background
Tenascin C is an extracellular matrix protein that is expressed in various tissues during development, disease or injury. Tenascin C inhibits HIV infection in immune cells by binding to a chemokine coreceptor site on the HIV-1 envelope protein, thereby blocking the virus' entry into the host cells. Tenascin C expression in the stroma of certain tumors is often associated with a poor prognosis.
Immunogen
Recombinant human Tenascin C [Swiss-Prot# P24821].
Localization
Secreted > extracellular space > extracellular matrix
Specificity
NB110-68136 specifically reacts with Domain B on FNIII repeats of Tenascin C.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNC
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 5 ug/ml
  • ELISA
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:25 - 1:50
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:25 - 1:50
  • CyTOF-ready
  • Flow (Intracellular)
Application Notes
See PMID: 21871891 for the ICC/IF use of thsi product. For its use in IHC-P, it is recommended to incubate primary antibody for atleast 2 hours at room temperature followed by ON incubation at 4C.
Reviewed Applications
Read 1 Review rated 3
using
NB110-68136 in the following applications:

Publications
Read Publications using
NB110-68136 in the following applications:

  • 2 publications
  • IHC
    5 publications
  • 1 publication
  • 5 publications
  • IP
    1 publication
  • WB
    4 publications

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Tenascin C Antibody (4C8MS)

  • 150-225
  • Cytotactin
  • Glioma-associated-extracellular matrix antigen
  • GMEM
  • GP 150-225
  • hexabrachion (tenascin C, cytotactin)
  • hexabrachion (tenascin)
  • Hexabrachion
  • HXB
  • HXBcytotactin
  • JI
  • MGC167029
  • Myotendinous antigen
  • neuronectin
  • Tenascin C
  • Tenascin J1
  • tenascin
  • tenascin-C isoform 14/AD1/16
  • Tenascin-C
  • TNC
  • TN-C
  • TNGP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready

Publications for Tenascin C Antibody (NB110-68136)(17)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ELISA, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ELISA
(2)
IHC
(5)
IHC-Fr
(1)
IHC-P
(5)
IP
(1)
WB
(4)
All Applications
Filter By Species
Human
(6)
Mouse
(2)
Rat
(6)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB110-68136 Applications Species
Janune D, Abd El Kader T, Aoyama E et al. Novel role of CCN3 that maintains the differentiated phenotype of articular cartilage. J. Bone Miner. Metab. Nov 16 2016 [PMID: 27853940] (WB, Rat) WB Rat
Bhattacharyya S, Wang W, Morales-Nebreda L et al. Tenascin-C drives persistence of organ fibrosis. Nat Commun Jun 3 2016 [PMID: 27256716] (WB, IP) WB, IP
de Sousa AP, Gurgel CA, Ramos EA et al. Infrared LED light therapy influences the expression of fibronectin and tenascin in skin wounds of malnourished rats-A preliminary study. Acta Histochem. 2014 Jul 12 [PMID: 25028133] (IHC-P, Rat, Human)

Details:
Tenascin C antibody used for IHC-P on skin wounds of malnourished male albino Wistar rats (Rattus norvegicus) - antigen retreival using 1% trypsin solution at 37C for 30 min, primary incubation 60 minutes at 1:50 dilution, detection using EnVision(TM) Polymer - DAB, sections of human placenta tissue used as positive control (Fig 1).
IHC-P Rat, Human
James ML, Ross AC, Nicola T et al. VARA Attenuates Hyperoxia-Induced Impaired Alveolar Development and Lung Function in Newborn Mice. Am J Physiol Lung Cell Mol Physiol 2013 Apr 12 [PMID: 23585226] (WB, Mouse) WB Mouse
Ago K, Kemmochi S, Morita R et al. Direct progression of capsular invasive carcinomas from subcapsular focal hyperplasias induced by hypothyroidism-mediated tumor promotion in a rat two-stage thyroid carcinogenesis model J Cancer Res Clin Oncol 2012 Nov 1 [PMID: 23114882] (IHC, Rat) IHC Rat
Kemmochi S, Yamamichi S, Shimamoto K et al. Lac color inhibits development of rat thyroid carcinomas through targeting activation of plasma hyaluronan-binding protein Exp Biol Med (Maywood) 2012 Jun [PMID: 22715430] (IHC, Rat) IHC Rat
Kemmochi S, Shimamoto K, Shiraki A et al. Promoting effects of carminic acid-enriched cochineal extracts on capsular invasive thyroid carcinomas through targeting activation of angiogenesis in rats J Toxicol Sci 2012 [PMID: 22687987] (IHC, Rat) IHC Rat
Janune D, Kubota S, Nishida T et al. Novel effects of CCN3 that may direct the differentiation of chondrocytes. FEBS Lett. 2011 Aug 24. [PMID: 21871891]
Yamamoto M, Okui N, Tatebe M, Shinohara T, Hirata H. Regeneration of the perineurium after microsurgical resection examined with immunolabeling for tenascin-C and alpha smooth muscle actin. J Anat;218(4):413-25. 2011 Apr. [PMID: 21265831] (IHC-P, Rat) IHC-P Rat
Balasenthil S, Chen N, Lott ST, Chen J, Carter J, Grizzle WE, Frazier ML, Sen S, Killary AM. A migration signature and plasma biomarker panel for pancreatic adenocarcinoma. Cancer Prev Res (Phila);4(1):137-49. 2011 Jan. [PMID: 21071578] (ELISA, Human) ELISA Human
Show All 17 Publications.

Review for Tenascin C Antibody (NB110-68136) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ICC.

Reviews using NB110-68136:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Tenascin C NB110-68136
Enlarge
reviewed by:
Anonymous
ICC Human 03/22/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedUterus tissue
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tenascin C Antibody (NB110-68136). (Showing 1 - 1 of 1 FAQs).

  1. One of our clients is looking for antibodies against tenascin C and fibronectin that could be used for FFPE rat tissues. These antibodies should exclusively recognize tenascin C and fibronectin and do not cross react with each other. Can you inform me if you have these antibodies?
    • Here is a link to Tenascin C antibodies we have validated in rat tissues for IHC-P Here are antibodies we have validated in fibronectin in rat tissues for IHC-P

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-68136AF405
Alexa Fluor 488 Labeled NB110-68136AF488
Alexa Fluor 647 Labeled NB110-68136AF647
Alexa Fluor 700 Labeled NB110-68136AF700
Allophycocyanin Labeled NB110-68136APC
Biotin Labeled NB110-68136B
DyLight 350 Labeled NB110-68136UV
DyLight 405 Labeled NB110-68136V
DyLight 488 Labeled NB110-68136G
DyLight 550 Labeled NB110-68136R
DyLight 650 Labeled NB110-68136C
DyLight 680 Labeled NB110-68136FR
DyLight 755 Labeled NB110-68136IR
FITC Labeled NB110-68136F
HRP Labeled NB110-68136H
PE Labeled NB110-68136PE
PerCP Labeled NB110-68136PCP

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Recent Reviews

3
5
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4
0
3
1
2
0
1
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Anonymous
03/22/2017
Application: ICC
Species: Human

Bioinformatics

Gene Symbol TNC
Entrez
Uniprot