Tenascin C Antibody (4C8MS) - BSA Free

Images

 
Biological Strategies: Flow (Intracellular): Tenascin C Antibody (4C8MS) [NB110-68136] - Figure A: Intracellular stain performed on U87MG Cells with Tenascin C (4C8MS) antibody NB110-68136 (blue) and a matched ...read more
Biological Strategies: Western Blot: Tenascin C Antibody (4C8MS) [NB110-68136] - Regulation of Tenascin C expression and its effect on fibrotic responses. Confluent foreskin fibroblasts were incubated with ...read more
Immunocytochemistry/ Immunofluorescence: Tenascin C Antibody (4C8MS) [NB110-68136] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. ...read more
Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [NB110-68136] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone-tendon using Tenascin C antibody (clone 4C8MS) at 1:25 dilution. ...read more
Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136] - An intracellular stain was performed on MCF7 cells with Tenascin C Antibody (4C8MS) NB110-68136 (blue) and a matched isotype control MAB002 ...read more
Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [NB110-68136] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone-tendon using Tenascin C antibody (clone 4C8MS) at 1:25 dilution. ...read more
Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Tenascin C antibody (dark blue). Isotype control shown in orange. An antibody ...read more
Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody (4C8MS) NB110-68136 and a matched isotype control. Cells were fixed with 4% ...read more
Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody (4C8MS) NB110-68136AF488 (blue) and a matched isotype control (orange). Cells ...read more
Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody [4C8MS] NB110-68136AF647 (blue) and a matched isotype control (orange). Cells ...read more
Regulation of tenascin-C expression and its effect on fibrotic responses.(a,b) Confluent foreskin fibroblasts were incubated with TGF-beta (10 ng ml-1 or indicated concentrations) or tenascin-C (TNC) for 24 or 72 h (b) ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, FeSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
4C8MS
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Tenascin C Antibody (4C8MS) - BSA Free Summary

Background
Tenascin C is an extracellular matrix protein that is expressed in various tissues during development, disease or injury. Tenascin C inhibits HIV infection in immune cells by binding to a chemokine coreceptor site on the HIV-1 envelope protein, thereby blocking the virus' entry into the host cells. Tenascin C expression in the stroma of certain tumors is often associated with a poor prognosis.
Immunogen
Recombinant human Tenascin C [Swiss-Prot# P24821].
Localization
Secreted > extracellular space > extracellular matrix
Specificity
NB110-68136 specifically reacts with Domain B on FNIII repeats of Tenascin C.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNC
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Flow (Intracellular)
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 5 ug/ml
Application Notes
For use in IHC-P, it is recommended to incubate primary antibody for at least 2 hours at room temperature followed by ON incubation at 4C. This antibody is CyTOF ready.
Reviewed Applications
Read 2 Reviews rated 3.5
using
NB110-68136 in the following applications:

Publications
Read Publications using
NB110-68136 in the following applications:

Reactivity Notes

Feline reactivity reported in customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Tenascin C Antibody (4C8MS) - BSA Free

  • 150-225
  • Cytotactin
  • Glioma-associated-extracellular matrix antigen
  • GMEM
  • GP 150-225
  • hexabrachion (tenascin C, cytotactin)
  • hexabrachion (tenascin)
  • Hexabrachion
  • HXB
  • HXBcytotactin
  • JI
  • MGC167029
  • Myotendinous antigen
  • neuronectin
  • Tenascin C
  • Tenascin J1
  • tenascin
  • tenascin-C isoform 14/AD1/16
  • Tenascin-C
  • TNC
  • TN-C
  • TNGP

Background

Tenascin, also known as hexabrachion and cytotactin, is an extracellular matrix protein with a spatially and temporally restricted tissue distribution. It is a hexameric, multidomain protein with disulfide linked subunits of 190 to 240 kD, originally characterized as 'myotendinous antigen.' In the embryo it is present in dense mesenchyme surrounding developing epithelia and in developing cartilage and bone. In the adult, tenascin remains present in tendons and myotendinous junctions in the perichondrium and periosteum, as well as in smooth muscle. Tenascin (TN)(Erickson and Bourdon 1989, Erickson 1993) is a high molecular weight, multifunctional, extracellular matrix glycoprotein, expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1-200/220, myotendinous antigen (MI), neuronectin (NEC1) and glioma mesenchymal extracellular matrix (GMEM). The TN molecule is a disulfide-linked hexamer. Human TN has 3 subunits of 190, 200 and 220 kDa. It is primarily made up of 14.5 epidermal growth-factor-like repeats, 15 units similar to the fibronectin type-III-homology repeat and, at the C-terminus, has a sequence with homology to the globular domain of the beta and gamma chain of fibrinogen. Monoclonal antibody reacting specifically with tenascin is an essential tool for the localization, identification and studies on the role of the molecule in epithelial-mesenchymal and neuronal-glial interactions (Castellucci et al. 1991, Balza et al. 1993).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tenascin C Antibody (NB110-68136)(26)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: ELISA, FLOW, ICC/IF, IF/IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ELISA
(2)
FLOW
(1)
ICC/IF
(1)
IF/IHC
(7)
IHC-Fr
(1)
IHC-P
(6)
WB
(5)
All Applications
Filter By Species
Human
(9)
Mouse
(3)
Rat
(8)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using NB110-68136 Applications Species
Li J, Sina AAI, Antaw F et al. Digital Decoding of Single Extracellular Vesicle Phenotype Differentiates Early Malignant and Benign Lung Lesions Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022-11-17 [PMID: 36394090] (FLOW, Human) FLOW Human
Ni Y, Wu GH, Cai JJ et al. Tubule-mitophagic secretion of SerpinG1 reprograms macrophages to instruct anti-septic acute kidney injury efficacy of high-dose ascorbate mediated by NRF2 transactivation International journal of biological sciences 2022-08-08 [PMID: 35982894] (WB) WB
Louisthelmy R, Burke BM, Cornelison RC Brain cancer cell-derived matrices and effects on astrocyte migration Cells, tissues, organs 2022-02-15 [PMID: 35168244]
Dorchin-Ashkenazi H, Ginat-Koton R, Gabet Y et al. The Balance between Orthodontic Force and Radiation in the Jawbone: Microstructural, Histological, and Molecular Study in a Rat Model Biology (Basel) 2021-11-18 [PMID: 34827196] (IHC-P, Rat) IHC-P Rat
Lee Y, Ricky S, Lim TH, et al. An atmospheric plasma jet induces expression of wound healing genes in progressive burn wounds in a comb burn rat model: a pilot study Journal of burn care & research : official publication of the American Burn Association 2021-01-22 [PMID: 33482000]
Otsuka T, Mengsteab PY, Laurencin CT Control of mesenchymal cell fate via application of FGF-8b in vitro Stem cell research 2021-01-07 [PMID: 33445073] (ICC/IF, Rat) ICC/IF Rat
Haydont V, Neiveyans V, Perez P et al. Fibroblasts from the Human Skin Dermo-Hypodermal Junction are Distinct from Dermal Papillary and Reticular Fibroblasts and from Mesenchymal Stem Cells and Exhibit a Specific Molecular Profile Related to Extracellular Matrix Organization and Modeling Cells 2020-02-05 [PMID: 32033496] (IF/IHC, Human) IF/IHC Human
Ishikawa K, Kohno RI, Mori K Increased expression of periostin and tenascin-C in eyes with neovascular glaucoma secondary to PDR Graefes Arch. Clin. Exp. Ophthalmol. 2019-12-20 [PMID: 31863397]
Becerril S, Rodriguez A, Catalan V, Mendez-Gimenez L. Targeted disruption of the iNOS gene improves adipose tissue inflammation and fibrosis in leptin-deficient ob/ob mice: role of tenascin C. Int J Obes (Lond). 2018-02-15 [PMID: 29449623] (IF/IHC, Mouse) IF/IHC Mouse
Janune D, Abd El Kader T, Aoyama E et al. Novel role of CCN3 that maintains the differentiated phenotype of articular cartilage. J. Bone Miner. Metab. 2016-11-16 [PMID: 27853940] (WB, Rat) WB Rat
Show All 26 Publications.

Reviews for Tenascin C Antibody (NB110-68136) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Feline.

Reviews using NB110-68136:
Filter by Applications
IHC-Fr
(1)
ICC
(1)
All Applications
Filter by Species
Human
(1)
Feline
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
IHC-Fr Feline 09/15/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSclera
SpeciesFeline
LotC-1
Immunocytochemistry Tenascin C NB110-68136
Enlarge
3
reviewed by:
Verified Customer
ICC Human 03/22/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedUterus tissue
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tenascin C Antibody (NB110-68136). (Showing 1 - 2 of 2 FAQs).

  1. One of our clients is looking for antibodies against tenascin C and fibronectin that could be used for FFPE rat tissues. These antibodies should exclusively recognize tenascin C and fibronectin and do not cross react with each other. Can you inform me if you have these antibodies?
    • Here is a link to Tenascin C antibodies we have validated in rat tissues for IHC-P Here are antibodies we have validated in fibronectin in rat tissues for IHC-P
  2. I would like to see image of WB to see the isoforms of the tenascin NB110-68136.
    • I am afraid we do not have notes or images on the westerns for this antibody. There are 2 publications that do have a double band in westerns for this antibody: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3680762/. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1868127/. We have 2 Tenascin C antibodies that have been validated in mouse for western blot but none with images.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

3.5
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1
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0
1
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Verified Customer
09/15/2018
Application: IHC-Fr
Species: Feline

Verified Customer
03/22/2017
Application: ICC
Species: Human

Bioinformatics

Gene Symbol TNC
Entrez
Uniprot