Pericentrin Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Analysis in human testis and liver tissues. Corresponding Pericentrin RNA-seq data are presented for the same tissues.
Independent Antibodies: Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human cerebral cortex, liver, placenta and testis using Anti-Pericentrin antibody NBP1-87772 (A) shows ...read more
Immunocytochemistry/ Immunofluorescence: Pericentrin Antibody [NBP1-87772] - Smear of -20C methanol-fixed mouse small intestinal epithelial cells stained for ZO-1 (red) and pericentrin (green). ICC/IF image submitted by ...read more
Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunocytochemistry/ Immunofluorescence: Pericentrin Antibody [NBP1-87772] - Staining of human cell line A-431 shows localization to centrosome. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human testis shows high expression.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining in human testis and liver tissues using anti-PCNT antibody. Corresponding PCNT RNA-seq data are presented for the ...read more
Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Pericentrin Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MEDLQNQFQKELAEQRAELEKIFQDKNQAERALRNLESHHQAAIEKLREDLQSEHGRCLEDLEFKFKESEKEKQLELENLQASYEDLKAQSQEEIRRLWSQLDSARTSRQELSELHEQLLARTSRVEDLEQLKQREKTQHESELE
Marker
Centrosome Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PCNT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25 - 2 ug/mL
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Pericentrin antibody validated for ICC/IF from a verified customer review.
Control Peptide
Pericentrin Protein (NBP1-87772PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-87772 in the following applications:

Publications
Read Publications using
NBP1-87772 in the following applications:

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Pericentrin Antibody

  • Kendrin
  • KIAA0402
  • MOPD2
  • PCN
  • PCNT2pericentrin B
  • PCNTB
  • pericentrin 2 (kendrin)
  • pericentrin
  • pericentrin, kendrin (KIAA0402)10KENPCTN2
  • pericentrin-2
  • pericentrin-380
  • pericentrin-B
  • SCKL4kendrin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-87772
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P

Publications for Pericentrin Antibody (NBP1-87772)(3)

Review for Pericentrin Antibody (NBP1-87772) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-87772:
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Immunocytochemistry
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Pericentrin NBP1-87772
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5
reviewed by:
Verified Customer
Immunocytochemistry Mouse 08/16/2021
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Summary

ApplicationImmunocytochemistry
Sample TestedAdult small intestine
SpeciesMouse
LotA119322

Comments

CommentsWe tested the antibody on smears (cytology-like preps) of mouse small intestinal epithelial cells fixed with 2% paraformaldehyde/lysine/periodate (PLP; McLean and Nakane. J Histochem Cytochem, 1974) or -20°C methanol. After permeabilizing with 0.1% saponin (PLP-fixed only) and blocking with 5% donkey serum (in PBS), slides were incubated with antibodies at the dilutions indicated below and a rat monoclonal anti-ZO-1 (tight junction protein) antibody (clone R40.76) for 2 hours at room temperature. After washing, species-specific secondary antibodies (cross-adsorbed) produced in goat were applied.
***Novus Innovators Program - new species or application used on a primary antibody.

Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Pericentrin Antibody (NBP1-87772) (0)

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Secondary Antibodies

 

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Recent Reviews

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Verified Customer
08/16/2021
Application: Immunocytochemistry
Species: Mouse

Bioinformatics

Gene Symbol PCNT