Pericentrin Antibody

Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Analysis in human testis and liver tissues. Corresponding Pericentrin RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human cerebral cortex, liver, placenta and testis using Anti-Pericentrin antibody NBP1-87772 (A) shows similar protein distribution across tissues to independent antibody NBP1-87771 (B).

Immunocytochemistry/Immunofluorescence: Pericentrin Antibody [NBP1-87772] - Smear of -20C methanol-fixed mouse small intestinal epithelial cells stained for ZO-1 (red) and pericentrin (green). ICC/IF image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunocytochemistry/Immunofluorescence: Pericentrin Antibody [NBP1-87772] - Staining of human cell line A-431 shows localization to centrosome. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human testis shows high expression.

Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining in human testis and liver tissues using anti-PCNT antibody. Corresponding PCNT RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.

• Reactivity: Hu, Mu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Pericentrin Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: MEDLQNQFQKELAEQRAELEKIFQDKNQAERALRNLESHHQAAIEKLREDLQSEHGRCLEDLEFKFKESEKEKQLELENLQASYEDLKAQSQEEIRRLWSQLDSARTSRQELSELHEQLLARTSRVEDLEQLKQREKTQHESELE
• Marker: Centrosome Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PCNT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25 - 2 ug/mL
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Pericentrin antibody validated for ICC/IF from a verified customer review.
• Control Peptide:

  Pericentrin Protein (NBP1-87772PEP)

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Pericentrin Antibody

• Kendrin
• KIAA0402
• MOPD2
• PCN
• PCNT2pericentrin B
• PCNTB
• pericentrin 2 (kendrin)
• pericentrin
• pericentrin, kendrin (KIAA0402)10KENPCTN2
• pericentrin-2
• pericentrin-380
• pericentrin-B
• SCKL4kendrin

Background

Pericentrin is encoded by this gene binds to calmodulin and is expressed in the centrosome. It is an integral component of the pericentriolar material (PCM). The protein contains a series of coiled-coil domains and a highly conserved PCM targeting motif called the PACT domain near its C-terminus. The protein interacts with the microtubule nucleation component gamma-tubulin and is likely important to normal functioning of the centrosomes, cytoskeleton, and cell-cycle progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pericentrin Antibody (NBP1-87772)(3)

Publications using NBP1-87772

• Dutto I, Gerhards J, Herrera A et al. Pathway-specific effects of ADSL deficiency on neurodevelopment eLife 2022-02-08 [PMID: 35133277] (ICC/IF, Human)
• Pasdeloup D, Labetoulle M, Rixon FJ et al. Differing Effects of Herpes Simplex Virus 1 and Pseudorabies Virus Infections on Centrosomal Function. J Virol 2013-06-01 [PMID: 23596303]
• Uhlen M, Oksvold P, Algenas C et al. Antibody-based Protein Profiling of the Human Chromosome 21. Mol Cell Proteomics 2012-03-01 [PMID: 22042635]

Review for Pericentrin Antibody (NBP1-87772) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer ICC Mouse 08/16/2021

Summary

• Application: Immunocytochemistry
• Sample Tested: Adult small intestine
• Species: Mouse
• Lot: A119322

Comments

• Comments: We tested the antibody on smears (cytology-like preps) of mouse small intestinal epithelial cells fixed with 2% paraformaldehyde/lysine/periodate (PLP; McLean and Nakane. J Histochem Cytochem, 1974) or -20°C methanol. After permeabilizing with 0.1% saponin (PLP-fixed only) and blocking with 5% donkey serum (in PBS), slides were incubated with antibodies at the dilutions indicated below and a rat monoclonal anti-ZO-1 (tight junction protein) antibody (clone R40.76) for 2 hours at room temperature. After washing, species-specific secondary antibodies (cross-adsorbed) produced in goat were applied.
  ***Novus Innovators Program - new species or application used on a primary antibody.

Bioinformatics

• Gene Symbol: PCNT