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AKAP9 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: AKAP9 Antibody [NBP1-89166] - Analysis in human small intestine and pancreas tissues. Corresponding AKAP9 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: AKAP9 Antibody [NBP1-89166] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: AKAP9 Antibody [NBP1-89166] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: AKAP9 Antibody [NBP1-89166] - Staining of human cerebral cortex shows moderate granular positivity in cytoplasm granular in neurons.
Immunohistochemistry-Paraffin: AKAP9 Antibody [NBP1-89166] - Staining of human pancreas shows weak cytoplasmic granular positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: AKAP9 Antibody [NBP1-89166] - Staining of human placenta shows moderate granular positivity in cytoplasm in trophoblastic cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

AKAP9 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KLLEAISETSSQLEHAKVTQTELMRESFRQKQEATESLKCQEELRERLHEESRAREQLAVELSKAEGVIDGYADEKTLFERQIQEKTDIIDRLEQELLCASNRLQELEAEQQQIQEERELLSRQKEAMKAEAGPVEQQLLQETE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AKAP9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot Reported in scientific literature (PMID 25366663)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
AKAP9 Protein (NBP1-89166PEP)
Publications
Read Publications using
NBP1-89166 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (86%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for AKAP9 Antibody

  • A kinase (PRKA) anchor protein (yotiao) 9
  • AKAP 350
  • AKAP 450
  • AKAP350A-kinase anchor protein 450 kDa
  • AKAP450A-kinase anchor protein 350 kDa
  • AKAP-9
  • Centrosome- and Golgi-localized PKN-associated protein
  • CG-NAPAKAP 120-like protein
  • hgAKAP 350
  • HYPERION
  • KIAA0803A-kinase anchor protein 9
  • MU-RMS-40.16A
  • PRKA9AKAP9-BRAF fusion protein
  • Protein hyperion
  • protein kinase A anchoring protein 9
  • Protein kinase A-anchoring protein 9
  • Protein yotiao
  • YOTIAOkinase N-associated protein

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternate splicing of this gene results in many isoforms that localize to the centrosome and the Golgi apparatus, and interact with numerous signaling proteins from multiple signal transduction pathways. These signaling proteins include type II protein kinase A, serine/threonine kinase protein kinase N, protein phosphatase 1, protein phosphatase 2a, protein kinase C-epsilon and phosphodiesterase 4D3. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AKAP9 Antibody (NBP1-89166)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol AKAP9