Cdc20 Antibody

Images

 
Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human and Mouse CDC20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: ...read more
Immunohistochemistry: Cdc20 Antibody [NB100-59828] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB staining ...read more
Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human CDC20 on HeLa whole cell lysate using NB100-59828. CDC20 was also immunoprecipitated by rabbit anti-CDC20 antibody NB100-59827.
Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:250.
Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828] - Human breast carcinoma (left) and mouse teratoma (right). Antibody used at a dilution of 1:1,000 (0.2ug/ml).
Immunohistochemistry: Cdc20 Antibody [NB100-59828] - Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB staining using ...read more
Proximity Ligation Assay: Cdc20 Antibody [NB100-59828] - Image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

Cdc20 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 449 and 499 of human cell division cycle 20 using the numbering given in entry NP_001246.2 (GeneID 991).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDC20
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 mg/mg
  • Proximity Ligation Assay 1:200-1:4000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Positive Control
Cdc20 Lysate (NBL1-08992)
Publications
Read Publications using
NB100-59828 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cdc20 Antibody

  • CDC20 cell division cycle 20 homolog (S. cerevisiae)
  • CDC20 cell division cycle 20 homolog
  • cell division cycle 20 homolog (S. cerevisiae)
  • cell division cycle protein 20 homolog
  • MGC102824
  • p55CDCS. cerevisiae, homolog)

Background

CDC20 is a WD repeat containing cell cycle-regulator that binds to and activates the ubiquitin-ligase activity of the APC/c (anaphase-promoting complex/cyclosome). APC/c ubiquitinating activity is responsible for the degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. CDC20 has also been reported to form a complex with histone deacetylases and can function as a transcriptional repressor involved in cell cycle arrest. As a regulator of APC/c activity, CDC20 plays a key role in the spindle-assembly checkpoint.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cdc20 Antibody (NB100-59828)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CDC20
Entrez
Uniprot