Cytochrome P450 3A4 Antibody (3H8)

Images

 
Western Blot: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Western blot analysis using CYP3A4 mAb against HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Immunofluorescence analysis of HepG2 cells using CYP3A4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments ...read more
Immunohistochemistry: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Immunohistochemical analysis of paraffin-embedded human brain tissues using CYP3A4 mouse mAb with DAB staining.
Flow Cytometry: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Flow cytometric analysis of HepG2 cells using CYP3A4 mouse mAb (green) and negative control (red).
Western Blot: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Western blot analysis using CYP3A4 mAb against human CYP3A4 (AA: 243-430) recombinant protein. (Expected MW is 47.5 kDa)
Immunohistochemistry: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using CYP3A4 mouse mAb with DAB staining.
ELISA: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
Clone
3H8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Cytochrome P450 3A4 Antibody (3H8) Summary

Immunogen
Purified recombinant fragment of human CYP3A4 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CYP3A4
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • CyTOF-ready
Theoretical MW
57.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Cytochrome P450 3A4 Antibody (3H8)

  • Albendazole Monooxygenase
  • Albendazole Sulfoxidase
  • CP33
  • CP34
  • CYP3A
  • CYP3A3
  • CYP3A4
  • CYPIIIA3
  • CYPIIIA4
  • Cytochrome P450 3A3
  • cytochrome P450 3A4
  • Cytochrome P450 HLp
  • Cytochrome P450 NF-25
  • cytochrome P450, family 3, subfamily A, polypeptide 4
  • cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3
  • cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4
  • Cytochrome P450-PCN1
  • EC 1.14.13.32
  • EC 1.14.13.67
  • EC 1.14.13.97
  • EC 1.14.14.1
  • glucocorticoid-inducible P450
  • HLP
  • MGC126680
  • NF-25
  • Nifedipine oxidase
  • P450C3
  • P450-III, steroid inducible
  • P450PCN1
  • Quinine 3-Monooxygenase
  • Taurochenodeoxycholate 6-Alpha-Hydroxylase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CYP3A4
Entrez