Immunohistochemistry: Pericentrin Antibody [NB100-61071] - Immunofluorescent analysis of cilia in renal tissues. Sections (4 um) of renal parenchymal tissues and tumor tissues were stained with DAPI, ...read more
Biological Strategies: Western Blot: Pericentrin Antibody [NB100-61071] - Detection of Human Pericentrin/Kendrin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN ...read more
Immunocytochemistry/ Immunofluorescence: Pericentrin Antibody [NB100-61071] - Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Pericentrin/Kendrin used at a dilution of 1:1,000 ...read more
Immunoprecipitation: Pericentrin Antibody [NB100-61071] - Detection of human Pericentrin/Kendrin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from ...read more
IHC-P was reported in scientific literature (PMID: 23369289). ICC/IF reactivity reported in scientific literature (PMID: 24316073). ICC/IF Formaldehyde or Acetone fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
378 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Pericentrin (PCNT) is a large centrosomal protein that binds calmodulin and mediates assembly of the mitotic spindle apparatus. Depletion of PCNT has been shown to cause apoptosis and activation of the G2/M checkpoint. Recently, mutations in the pericentrin gene have been identified as the cause of primordial dwarfism type II (MOPDII) and Seckel syndrome. Additionally, in cells of Seckel syndrome patients, PCNT has been found to be important to the DNA damage response and ATR-dependent checkpoint signaling.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Pericentrin Antibody (NB100-61071). (Showing 1 - 1 of 1 FAQs).
I ordered your antibody 3 weeks ago and I received it today. The product is Pericentrin Antibody (NB100-61071) (0.1 mg). I did not have experience for doing immunofluorescence with this antibody before. I note that you used acetone or NBF to fix cell in the manual of your product. But the description is too simple, so could you send me a more detailed protocol about doing immunofluorescence with pericentrin antibody?
Please see the following link for our ICC protocol. I hope this helps!
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.