Pericentrin Antibody


Immunohistochemistry: Pericentrin Antibody [NB100-61071] - Immunofluorescent analysis of cilia in renal tissues. Sections (4 um) of renal parenchymal tissues and tumor tissues were stained with DAPI, more
Biological Strategies: Western Blot: Pericentrin Antibody [NB100-61071] - Detection of Human Pericentrin/Kendrin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN more
Immunocytochemistry/ Immunofluorescence: Pericentrin Antibody [NB100-61071] - Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Pericentrin/Kendrin used at a dilution of 1:1,000 more
Immunoprecipitation: Pericentrin Antibody [NB100-61071] - Detection of human Pericentrin/Kendrin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from more

Product Details

Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
1.0 mg/ml
Validated by:

Biological Strategies


Pericentrin Antibody Summary

The immunogen recognized by this antibody maps to a region between residue 175 and 225 of human Pericentrin/Kendrin using the numbering given in entry NP_006022.3 (GeneID 5116).
Centrosome Marker
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:2000 - 1:10000
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-10 ug/mg lysate
Application Notes
IHC-P was reported in scientific literature (PMID: 23369289). ICC/IF reactivity reported in scientific literature (PMID: 24316073). ICC/IF Formaldehyde or Acetone fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Theoretical MW
378 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Pericentrin Knockout HeLa Cell Lysate
Pericentrin Knockout 293T Cell Lysate
Read Publications using
NB100-61071 in the following applications:

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for Pericentrin Antibody

  • Kendrin
  • KIAA0402
  • MOPD2
  • PCN
  • PCNT2pericentrin B
  • pericentrin 2 (kendrin)
  • pericentrin
  • pericentrin, kendrin (KIAA0402)10KENPCTN2
  • pericentrin-2
  • pericentrin-380
  • pericentrin-B
  • SCKL4kendrin


Pericentrin (PCNT) is a large centrosomal protein that binds calmodulin and mediates assembly of the mitotic spindle apparatus. Depletion of PCNT has been shown to cause apoptosis and activation of the G2/M checkpoint. Recently, mutations in the pericentrin gene have been identified as the cause of primordial dwarfism type II (MOPDII) and Seckel syndrome. Additionally, in cells of Seckel syndrome patients, PCNT has been found to be important to the DNA damage response and ATR-dependent checkpoint signaling.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pericentrin Antibody (NB100-61071)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, IHC-P.

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Showing Publications 1 - 7 of 7.
Publications using NB100-61071 Applications Species
Cuenca A, Insinna C, Zhao H et al. The C7orf43/TRAPPC14 component links the TRAPPII complex to RABIN8 for preciliary vesicle tethering at the mother centriole during ciliogenesis J. Biol. Chem. Aug 29 2019 [PMID: 31467083] (ICC/IF, Human) ICC/IF Human
Walia V, Cuenca A, Vetter M et al. Akt Regulates a Rab11-Effector Switch Required for Ciliogenesis Dev Cell. May 31 2019 [PMID: 31204173]
Motuhi SE, Feizbakhsh O, Foll-Josselin B et al. Neurymenolide A, a Novel Mitotic Spindle Poison from the New Caledonian Rhodophyta Phacelocarpus neurymenioides Mar Drugs Feb 1 2019 [PMID: 30717235] (ICC/IF, Human) ICC/IF Human
Insinna C, Lu Q, Teixeira I et al. Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport. Nat Commun Jan 25 2019 [PMID: 30683896] (ICC/IF, Human) ICC/IF Human
Xia X, Gholkar A, Senese S, Torres JZ. A LCMT1-PME-1 Methylation Equilibrium Controls Mitotic Spindle Size Cell Cycle 2015 Apr 03 [PMID: 25839665] (ICC/IF, Human)

Pericentrin antibody was used for ICC-IF on HeLa cells which were transfected with si-Control, siLCMT1, or siPME-1 for 48 hours (Fig 1B).
ICC/IF Human
Loktev AV, Jackson PK. Neuropeptide Y Family Receptors Traffic via the Bardet-Biedl Syndrome Pathway to Signal in Neuronal Primary Cilia. Cell Rep 2013 Dec 12 [PMID: 24316073] (ICC/IF, Human) ICC/IF Human
Basten SG, Willekers S, Vermaat JS et al. Reduced cilia frequencies in human renal cell carcinomas versus neighboring parenchymal tissue. Cilia 2013 Jan 31 [PMID: 23369289] (IHC-P, ICC/IF, Human) IHC-P, ICC/IF Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Pericentrin Antibody (NB100-61071). (Showing 1 - 1 of 1 FAQs).

  1. I ordered your antibody 3 weeks ago and I received it today. The product is Pericentrin Antibody (NB100-61071) (0.1 mg). I did not have experience for doing immunofluorescence with this antibody before. I note that you used acetone or NBF to fix cell in the manual of your product. But the description is too simple, so could you send me a more detailed protocol about doing immunofluorescence with pericentrin antibody?
    • Please see the following link for our ICC protocol. I hope this helps!

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-61071AF350
Alexa Fluor 405 NB100-61071AF405
Alexa Fluor 488 NB100-61071AF488
Alexa Fluor 532 NB100-61071AF532
Alexa Fluor 594 NB100-61071AF594
Alexa Fluor 647 NB100-61071AF647
Alexa Fluor 700 NB100-61071AF700
Alexa Fluor 750 NB100-61071AF750
Biotin NB100-61071B
DyLight 350 NB100-61071UV
DyLight 405 NB100-61071V
DyLight 488 NB100-61071G
DyLight 550 NB100-61071R
DyLight 594 NB100-61071DL594
DyLight 650 NB100-61071C
DyLight 680 NB100-61071FR
DyLight 755 NB100-61071IR
FITC NB100-61071F
HRP NB100-61071H
Janelia Fluor 549 NB100-61071JF549
Janelia Fluor 646 NB100-61071JF646

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Gene Symbol PCNT