Biological Strategies: Western Blot: Pericentrin Antibody [NB100-61071] - Detection of Human Pericentrin/Kendrin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN ...read more
Immunocytochemistry/ Immunofluorescence: Pericentrin Antibody [NB100-61071] - Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Pericentrin/Kendrin used at a dilution of 1:1,000 ...read more
Immunohistochemistry-Paraffin was reported in scientific literature (PMID: 23369289). ICC/IF reactivity reported :in scientific literature (PMID: 24316073). Immunofluorescence (ICC) 1:1,000 - 1:5,000. Formaldehyde or Acetone fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
378 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Pericentrin (PCNT) is a large centrosomal protein that binds calmodulin and mediates assembly of the mitotic spindle apparatus. Depletion of PCNT has been shown to cause apoptosis and activation of the G2/M checkpoint. Recently, mutations in the pericentrin gene have been identified as the cause of primordial dwarfism type II (MOPDII) and Seckel syndrome. Additionally, in cells of Seckel syndrome patients, PCNT has been found to be important to the DNA damage response and ATR-dependent checkpoint signaling.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Pericentrin Antibody (NB100-61071). (Showing 1 - 1 of 1 FAQs).
I ordered your antibody 3 weeks ago and I received it today. The product is Pericentrin Antibody (NB100-61071) (0.1 mg). I did not have experience for doing immunofluorescence with this antibody before. I note that you used acetone or NBF to fix cell in the manual of your product. But the description is too simple, so could you send me a more detailed protocol about doing immunofluorescence with pericentrin antibody?
Please see the following link for our ICC protocol. I hope this helps!
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