Pericentrin Antibody

Images

 
Biological Strategies: Western Blot: Pericentrin Antibody [NB100-61071] - Detection of Human Pericentrin/Kendrin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN ...read more
Immunocytochemistry/ Immunofluorescence: Pericentrin Antibody [NB100-61071] - Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Pericentrin/Kendrin used at a dilution of 1:1,000 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Pericentrin Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 175 and 225 of human Pericentrin/Kendrin using the numbering given in entry NP_006022.3 (GeneID 5116).
Marker
Centrosome Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PCNT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-10 ug/mg lysate
Application Notes
Immunohistochemistry-Paraffin was reported in scientific literature (PMID: 23369289). ICC/IF reactivity reported :in scientific literature (PMID: 24316073). Immunofluorescence (ICC) 1:1,000 - 1:5,000. Formaldehyde or Acetone fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Theoretical MW
378 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Pericentrin Lysate (NBP2-64901)
Pericentrin Lysate (NBP2-65520)
Publications
Read Publications using
NB100-61071 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Pericentrin Antibody

  • Kendrin
  • KIAA0402
  • MOPD2
  • PCN
  • PCNT2pericentrin B
  • PCNTB
  • pericentrin 2 (kendrin)
  • pericentrin
  • pericentrin, kendrin (KIAA0402)10KENPCTN2
  • pericentrin-2
  • pericentrin-380
  • pericentrin-B
  • SCKL4kendrin

Background

Pericentrin (PCNT) is a large centrosomal protein that binds calmodulin and mediates assembly of the mitotic spindle apparatus. Depletion of PCNT has been shown to cause apoptosis and activation of the G2/M checkpoint. Recently, mutations in the pericentrin gene have been identified as the cause of primordial dwarfism type II (MOPDII) and Seckel syndrome. Additionally, in cells of Seckel syndrome patients, PCNT has been found to be important to the DNA damage response and ATR-dependent checkpoint signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-61816
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP2-37502
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
MAB1106
Species: Hu
Applications: WB, ELISA(Cap), ELISA(Det), ELISA(Sta)
H00003347-M01
Species: Hu
Applications: ELISA, ICC/IF
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-66987
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88795
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-15840
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
PP-N4111-00
Species: Hu
Applications: WB, IHC, IP
NBP1-89166
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-59828
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
AF748
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP1-87196
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NB100-128
Species: Hu, Mu, Rt
Applications: ChIP, GS, ICC/IF, IHC, IHC-P
NB100-74398
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-66958
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-01860
Species: Hu, Mu, Rt, Ca, Mk
Applications: WB, Flow, ICC/IF, IHC
NBP1-37003
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA
NB120-19347
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IF
NB100-61071
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP

Publications for Pericentrin Antibody (NB100-61071)(3)

Reviews for Pericentrin Antibody (NB100-61071) (0)

There are no reviews for Pericentrin Antibody (NB100-61071). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Pericentrin Antibody (NB100-61071). (Showing 1 - 1 of 1 FAQs).

  1. I ordered your antibody 3 weeks ago and I received it today. The product is Pericentrin Antibody (NB100-61071) (0.1 mg). I did not have experience for doing immunofluorescence with this antibody before. I note that you used acetone or NBF to fix cell in the manual of your product. But the description is too simple, so could you send me a more detailed protocol about doing immunofluorescence with pericentrin antibody?
    • Please see the following link for our ICC protocol. I hope this helps!

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Pericentrin Antibody (NB100-61071)

Discover related pathways, diseases and genes to Pericentrin Antibody (NB100-61071). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Pericentrin Antibody (NB100-61071)

Discover more about diseases related to Pericentrin Antibody (NB100-61071).
 

Pathways for Pericentrin Antibody (NB100-61071)

View related products by pathway.

PTMs for Pericentrin Antibody (NB100-61071)

Learn more about PTMs related to Pericentrin Antibody (NB100-61071).
 

Research Areas for Pericentrin Antibody (NB100-61071)

Find related products by research area.

Blogs on Pericentrin

There are no specific blogs for Pericentrin, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Pericentrin Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol PCNT
Entrez
Uniprot