Pax6 Antibody

Western Blot: Pax6 Antibody [NB300-750] - Analysis of sheep retinal extract

Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NB300-750] - ARPE-19 monolayer on glass substrate. Fixative Methanol Blocking Goat Serum. Dilution 1:100. Incubation 1h @ RT. This image was submitted via customer Review.

Western Blot: Pax6 Antibody [NB300-750] - Analysis of rat whole eye extract

Western Blot: Pax6 Antibody [NB300-750] - Analysis of various human, mouse and non-human primate whole cell lysates.

Western Blot: Pax6 Antibody [NB300-750] - Analysis of extracts from HeLa, U251 and mouse brain using PAX6 antibody (NB300-750, 1:100).

Western Blot: Pax6 Antibody [NB300-750] - Analysis of Pax6 in skate (Leucoraja erinacea) brain lysate using anti-Pax6 antibody. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NB300-750] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a PAX6 polyclonal antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin were stained with Hoechst 33342 dye.

Immunoprecipitation: Pax6 Antibody [NB300-750] - Analysis of PAX6 was performed on 293T cells. Antigen: antibody complexes were formed by incubating 500ug whole cell lysate with 5ug of PAX6 polyclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a PAX6 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:1000 for at least one hour.

• Reactivity: Hu, Mu, Rt, Fi, Pm, Pm, Sh
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Pax6 Antibody Summary

• Immunogen: Synthetic peptide corresponding to residues C R(267) E E K L R N Q R R Q A S N T P S H I(285) of mouse PAX6.
• Localization: Nuclear
• Marker: Neural Marker
• Specificity: Detects a ~47 kDa protein representing PAX6 (Schwann Cell Marker) from rat whole eye extracts and ovine retinal extracts.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAX6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry
  • Immunoprecipitation 5 ug
• Application Notes: Use in Immunohistochemistry reported in scientific literature (PMID: 22837015).
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Fish reactivity reported in scientific literature (PMID: 29888788).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Pax6 Antibody

• keratitis)
• MGC17209
• Oculorhombin
• paired box 6
• paired box protein Pax-6
• Pax6

Background

PAX genes encode nuclear transcription factors which are regarded as major controllers of developmental processes in both vertebrates and invertebrates. Mutations in murine PAX genes underlie three natural mouse alleles and several corresponding human syndromes (aniridia, foveal hypoplasia and Peters' anomaly). Murine PAX genes have been shown to be proto-oncogenes. Furthermore, human PAX genes have recently been demonstrated to play an influential part in some common human cancers such as brain tumors and lymphomas. All PAX genes encode a DNA-binding domain termed the paired domain and in addition some also encode a second binding domain--the paired type homeobox. PAX6 is involved in the early development of the optical vesicle and has been shown to interact with Six3, another important visual development protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pax6 Antibody (NB300-750)(4)

Publications using NB300-750

• Suriano CM. Developmental Evidence for the Evolutionary Relationship of Cerebellum and Cerebellum-like Structures in an Elasmobranch Fish. Thesis. 2018 (WB)
• Suriano CM, Bodznick D. Evidence for generative homology of cerebellum and cerebellum-like structures in an elasmobranch fish based on Pax6, Cbln1 and Grid2 expression J. Comp. Neurol. Jun 11 2018 [PMID: 29888788] (Fish)
• Imberti B, Tomasoni S, Ciampi O et al. Renal progenitors derived from human iPSCs engraft and restore function in a mouse model of acute kidney injury Sci Rep 2015 Mar 06 [PMID: 25744951] (ICC/IF, Human)
• Pacal M, Bremner R. Mapping differentiation kinetics in the mouse retina reveals an extensive period of cell cycle protein expression in post-mitotic newborn neurons Dev Dyn 2012 Oct [PMID: 22837015] (IHC, ICC/IF, Mouse)

Reviews for Pax6 Antibody (NB300-750) (4)

Average Rating: 4.5

(Based on 4 reviews)

We have 4 reviews tested in 2 species: Human, Other.

reviewed by: Fabio Marongiu ICC Human 08/29/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: ARPE-19 cells
• Species: Human
• Lot: RE227621

Comments

• Comments: Fixative Methanol
  Blocking Goat Serum
  Dilution 1:100
  Incubation 1h @ RT

reviewed by: Anonymous IF Other 04/20/2016

Summary

• Application: Immunofluorescence
• Sample Tested: Hindbrain
• Species: Other

Blocking

• Blocking Details: Blocked in 5% NGS, 0.1% Triton X in PBS

Secondary Antibody

• Secondary Description: anti-rabbit 488
• Secondary Concentration: varies

Details

• Detection Notes: Fluorescent Microscope
• Fixation Details: Fixed in 4%, PFA

Comments

• Comments: Worked in Leucoraja, not in Ambystoma and Petromyzon

reviewed by: Christos Suriano WB Other 02/09/2016

Summary

• Application: Western Blot
• Sample Tested: Skate brain
• Species: Other

Blocking

• Blocking Details: 1 Hour at RT in NGS

Details

• Detection Notes: HRP secondary with FEMTO

reviewed by: Anony- mous WB Human 06/13/2014

Summary

• Application: Western Blot
• Species: Human
• Special Applications: Western blot analysis of extracts from HeLa, U251 and mouse brain using PAX6 antibody (NB300-750, 1:100).
• File: View PDF

FAQs for Pax6 Antibody (NB300-750). (Showing 1 - 2 of 2 FAQs).

1. What PAX6 antibody is recommend to use in ICC staining of human stem cells differentiated neural precursors?
   • By performing a search on PAX6 then refining this to antibodies which have been validated with human samples and for ICC, I can see that we have three products to meet your requirements. You can see all three of these antibodies. These products are listed based on sales and citations, however all of our antibodies are covered by our 100% guarantee to work in the species and applications listed on our website and on our product datasheets. You can read more about our quality guarantee here.
2. I am just wondering if this specific epitope is conserved from rabbits to lampreys and skates
   • Yes, the immunogen is pretty conserved and is shared with the sequence available at Uniprot site so it may work in your mentioned species.

Bioinformatics

• Gene Symbol: PAX6
• Entrez:
  • 5080(Human)
  • 18508(Mouse)
  • 25509(Rat)
• Uniprot:
  • P63016(Rat)