Pax6 Antibody

Images

 
Western Blot: Pax6 Antibody [NB300-750] - Analysis of sheep retinal extract
Immunocytochemistry: Pax6 Antibody [NB300-750] - ARPE-19 monolayer on glass substrate. Fixative Methanol Blocking Goat Serum. Dilution 1:100. Incubation 1h @ RT. This image was submitted via customer Review.
Western Blot: Pax6 Antibody [NB300-750] - Analysis of rat whole eye extract
Western Blot: Pax6 Antibody [NB300-750] - Analysis of various human, mouse and non-human primate whole cell lysates.
Western Blot: Pax6 Antibody [NB300-750] - Analysis of extracts from HeLa, U251 and mouse brain using PAX6 antibody (NB300-750, 1:100).
Western Blot: Pax6 Antibody [NB300-750] - Analysis of Pax6 in skate (Leucoraja erinacea) brain lysate using anti-Pax6 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NB300-750] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 ...read more
Immunoprecipitation: Pax6 Antibody [NB300-750] - Analysis of PAX6 was performed on 293T cells. Antigen: antibody complexes were formed by incubating 500ug whole cell lysate with 5ug of PAX6 polyclonal antibody overnight ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Mk, Pm, ShSpecies Glossary
Applications WB, ICC/IF, IP, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Pax6 Antibody Summary

Immunogen
Synthetic peptide corresponding to residues C R(267) E E K L R N Q R R Q A S N T P S H I(285) of mouse PAX6.
Localization
Nuclear
Marker
Neural Marker
Specificity
Detects a ~47 kDa protein representing PAX6 (Schwann Cell Marker) from rat whole eye extracts and ovine retinal extracts.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PAX6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunoprecipitation 5 ug
  • Immunocytochemistry 1:100
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 4 Reviews rated 4.5
using
NB300-750 in the following applications:

Publications
Read Publications using
NB300-750 in the following applications:

  • 2 publications
  • IHC
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Pax6 Antibody

  • keratitis)
  • MGC17209
  • Oculorhombin
  • paired box 6
  • paired box protein Pax-6
  • Pax6

Background

PAX genes encode nuclear transcription factors which are regarded as major controllers of developmental processes in both vertebrates and invertebrates. Mutations in murine PAX genes underlie three natural mouse alleles and several corresponding human syndromes (aniridia, foveal hypoplasia and Peters' anomaly). Murine PAX genes have been shown to be proto-oncogenes. Furthermore, human PAX genes have recently been demonstrated to play an influential part in some common human cancers such as brain tumors and lymphomas. All PAX genes encode a DNA-binding domain termed the paired domain and in addition some also encode a second binding domain--the paired type homeobox. PAX6 is involved in the early development of the optical vesicle and has been shown to interact with Six3, another important visual development protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IP, ICC/IF

Publications for Pax6 Antibody (NB300-750)(4)

We have publications tested in 3 confirmed species: Human, Mouse, Fish.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(2)
IHC
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
Fish
(1)
All Species

Reviews for Pax6 Antibody (NB300-750) (4) 4.54

Average Rating: 4.5
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Other.

Reviews using NB300-750:
Filter by Applications
ICC
(1)
IF
(1)
WB
(2)
All Applications
Filter by Species
Human
(2)
Other
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Pax6 NB300-750
Enlarge
reviewed by:
Fabio Marongiu
ICC Human 08/29/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedARPE-19 cells
SpeciesHuman
LotRE227621

Comments

CommentsFixative Methanol
Blocking Goat Serum
Dilution 1:100
Incubation 1h @ RT
Immunofluorescence Pax6 NB300-750
Enlarge
reviewed by:
Anonymous
IF Other 04/20/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedHindbrain
SpeciesOther

Blocking

Blocking DetailsBlocked in 5% NGS, 0.1% Triton X in PBS

Secondary Antibody

Secondary Descriptionanti-rabbit 488
Secondary Concentrationvaries

Details

Detection NotesFluorescent Microscope
Fixation DetailsFixed in 4%, PFA

Comments

CommentsWorked in Leucoraja, not in Ambystoma and Petromyzon
Western Blot Pax6 NB300-750
Enlarge
reviewed by:
Christos Suriano
WB Other 02/09/2016
View

Summary

ApplicationWestern Blot
Sample TestedSkate brain
SpeciesOther

Blocking

Blocking Details1 Hour at RT in NGS

Details

Detection NotesHRP secondary with FEMTO
Western Blot Pax6 NB300-750
Enlarge
reviewed by:
Anony- mous
WB Human 06/13/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsWestern blot analysis of extracts from HeLa, U251 and mouse brain using PAX6 antibody (NB300-750, 1:100).
FileView PDF

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Pax6 Antibody (NB300-750). (Showing 1 - 2 of 2 FAQs).

  1. What PAX6 antibody is recommend to use in ICC staining of human stem cells differentiated neural precursors?
    • By performing a search on PAX6 then refining this to antibodies which have been validated with human samples and for ICC, I can see that we have three products to meet your requirements. You can see all three of these antibodies. These products are listed based on sales and citations, however all of our antibodies are covered by our 100% guarantee to work in the species and applications listed on our website and on our product datasheets. You can read more about our quality guarantee here.
  2. I am just wondering if this specific epitope is conserved from rabbits to lampreys and skates
    • Yes, the immunogen is pretty conserved and is shared with the sequence available at Uniprot site so it may work in your mentioned species.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Pax6 Antibody (NB300-750)

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Recent Reviews

4.5
5
2
4
2
3
0
2
0
1
0

Fabio Marongiu
08/29/2017
Application: ICC
Species: Human

Anonymous
04/20/2016
Application: IF
Species: Other

Christos Suriano
02/09/2016
Application: WB
Species: Other

Bioinformatics

Gene Symbol PAX6
Entrez
Uniprot