NeuroD1 Antibody [Unconjugated]

Neurogenic Differentiation factor 1 (NeuroD1) was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse NeuroD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2746) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

NeuroD1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human NeuroD1
  Ser154-Asp356
  Accession # Q13562
• Specificity: Detects NeuroD1 in direct ELISAs and Western blots. In these formats, approximately 5% cross-reactivity with recombinant human NeuroD2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NEUROD1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NeuroD1 Antibody [Unconjugated]

• basic helix-loop-helix transcription factor
• BETA2
• BHF-1
• BHLHA3
• bHLHa3beta-cell E-box transactivator 2
• Class A basic helix-loop-helix protein 3
• MODY6
• NeuroD
• NeuroD1
• neurogenic differentiation 1
• neurogenic differentiation factor 1
• neurogenic helix-loop-helix protein NEUROD
• NIDDM

Background

NeuroD1, also known as beta-cell E-box transactivator 2 (Beta 2), is a basic helix-loop-helix transcription factor. It is expressed during development in various neurons of the central and peripheral nervous systems. NeuroD1 is also expressed in beta-cells in the pancreas where it functions both as a transcriptional activator and repressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NeuroD1 Antibody (AF2746)(8)

Publications using AF2746

• M Jumabay, L Zhang, J Yao, KI Boström Progenitor cells from brown adipose tissue undergo neurogenic differentiation Scientific Reports, 2022-04-04;12(1):5614. 2022-04-04 [PMID: 35379860] (ICC, Mouse)
• NN Nawarawong, KR Thompson, SP Guerin, C Anasooya S, H Peng, K Nixon Reactive, Adult Neurogenesis From Increased Neural Progenitor Cell Proliferation Following Alcohol Dependence in Female Rats Frontiers in Neuroscience, 2021-09-14;15(0):689601. 2021-09-14 [PMID: 34594180] (IHC, Rat)
• H Liu, R Li, HK Liao, Z Min, C Wang, Y Yu, L Shi, J Dan, A Hayek, L Martinez M, E Nuñez Deli, JC Izpisua Be Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional &beta cells Nature Communications, 2021-06-07;12(1):3330. 2021-06-07 [PMID: 34099664] (IHC, Human)
• NJ Hogrebe, P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells Nat. Biotechnol., 2020-02-24;0(0):. 2020-02-24 [PMID: 32094658] (ICC, Human)
• Y Wang, L Lifshitz, K Gellatly, CL Vinton, K Busman-Sah, S McCauley, P Vangala, K Kim, A Derr, S Jaiswal, A Kucukural, P McDonel, PW Hunt, T Greenough, J Houghton, M Somsouk, JD Estes, JM Brenchley, M Garber, SG Deeks, J Luban HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells Nat. Immunol., 2020-02-17;21(3):274-286. 2020-02-17 [PMID: 32066947] (ICC, Human)
• N Sharon, J Vanderhoof, J Straubhaar, J Mueller, R Chawla, Q Zhou, EN Engquist, C Trapnell, DK Gifford, DA Melton Wnt Signaling Separates the Progenitor and Endocrine Compartments during Pancreas Development Cell Rep, 2019-05-21;27(8):2281-2291.e5. 2019-05-21 [PMID: 31116975] (ICC, Human)
• Cechin S, Alvarez-Cubela S, Giraldo J, Molano R, Villate S, Ricordi C, Pileggi A, Inverardi L, Fraker C, Dominguez-Bendala J Influence of in vitro and in vivo oxygen modulation on beta cell differentiation from human embryonic stem cells. Stem Cells Transl Med, 2013-12-27;3(3):277-89. 2013-12-27 [PMID: 24375542] (Flow Cytometry, Human)
• Farioli-Vecchioli S, Tanori M, Micheli L, Mancuso M, Leonardi L, Saran A, Ciotti MT, Ferretti E, Gulino A, Pazzaglia S, Tirone F Inhibition of medulloblastoma tumorigenesis by the antiproliferative and pro-differentiative gene PC3. FASEB J., 2007-03-19;21(9):2215-25. 2007-03-19 [PMID: 17371797] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: NEUROD1
• Uniprot:
  • Q13562()