NeuroD1 Antibody [Unconjugated]

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Neurogenic Differentiation factor 1 (NeuroD1) was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse NeuroD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2746) at ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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NeuroD1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human NeuroD1
Ser154-Asp356
Accession # Q13562
Specificity
Detects NeuroD1 in direct ELISAs and Western blots. In these formats, approximately 5% cross-reactivity with recombinant human NeuroD2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NEUROD1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2746 in the following applications:

  • ICC
    4 publications
  • IHC
    2 publications
  • 1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NeuroD1 Antibody [Unconjugated]

  • basic helix-loop-helix transcription factor
  • BETA2
  • BHF-1
  • BHLHA3
  • bHLHa3beta-cell E-box transactivator 2
  • Class A basic helix-loop-helix protein 3
  • MODY6
  • NeuroD
  • NeuroD1
  • neurogenic differentiation 1
  • neurogenic differentiation factor 1
  • neurogenic helix-loop-helix protein NEUROD
  • NIDDM

Background

NeuroD1, also known as beta-cell E-box transactivator 2 (Beta 2), is a basic helix-loop-helix transcription factor. It is expressed during development in various neurons of the central and peripheral nervous systems. NeuroD1 is also expressed in beta-cells in the pancreas where it functions both as a transcriptional activator and repressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NeuroD1 Antibody (AF2746)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC, IHC, IHC-P.


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ICC
(4)
IHC
(2)
IHC-P
(1)
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Filter By Species
Human
(4)
Mouse
(2)
Rat
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF2746 Applications Species
M Jumabay, L Zhang, J Yao, KI Boström Progenitor cells from brown adipose tissue undergo neurogenic differentiation Scientific Reports, 2022;12(1):5614. 2022 [PMID: 35379860] (ICC, Mouse) ICC Mouse
NN Nawarawong, KR Thompson, SP Guerin, C Anasooya S, H Peng, K Nixon Reactive, Adult Neurogenesis From Increased Neural Progenitor Cell Proliferation Following Alcohol Dependence in Female Rats Frontiers in Neuroscience, 2021;15(0):689601. 2021 [PMID: 34594180] (IHC, Rat) IHC Rat
H Liu, R Li, HK Liao, Z Min, C Wang, Y Yu, L Shi, J Dan, A Hayek, L Martinez M, E Nuñez Deli, JC Izpisua Be Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional &beta cells Nature Communications, 2021;12(1):3330. 2021 [PMID: 34099664] (IHC, Human) IHC Human
NJ Hogrebe, P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells Nat. Biotechnol., 2020;0(0):. 2020 [PMID: 32094658] (ICC, Human) ICC Human
Y Wang, L Lifshitz, K Gellatly, CL Vinton, K Busman-Sah, S McCauley, P Vangala, K Kim, A Derr, S Jaiswal, A Kucukural, P McDonel, PW Hunt, T Greenough, J Houghton, M Somsouk, JD Estes, JM Brenchley, M Garber, SG Deeks, J Luban HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells Nat. Immunol., 2020;21(3):274-286. 2020 [PMID: 32066947] (ICC, Human) ICC Human
N Sharon, J Vanderhoof, J Straubhaar, J Mueller, R Chawla, Q Zhou, EN Engquist, C Trapnell, DK Gifford, DA Melton Wnt Signaling Separates the Progenitor and Endocrine Compartments during Pancreas Development Cell Rep, 2019;27(8):2281-2291.e5. 2019 [PMID: 31116975] (ICC, Human) ICC Human
Farioli-Vecchioli S, Tanori M, Micheli L, Mancuso M, Leonardi L, Saran A, Ciotti MT, Ferretti E, Gulino A, Pazzaglia S, Tirone F Inhibition of medulloblastoma tumorigenesis by the antiproliferative and pro-differentiative gene PC3. FASEB J., 2007;21(9):2215-25. 2007 [PMID: 17371797] (IHC-P, Mouse) IHC-P Mouse

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Product General Protocols

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Bioinformatics

Gene Symbol NEUROD1
Uniprot