Islet-1 Antibody [Unconjugated]

Images

 
Simple Western lane view shows lysates of JOY6 human induced pluripotent stem cells undifferentiated and differentiated into motor neurons, loaded at 0.2 mg/mL. A specific band was detected for Islet‑1 at ...read more
Western blot shows lysates of JOY6 human induced pluripotent stem cells undifferentiated and differentiatied into motor neurons. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Islet‑1 Antigen ...read more
Islet‑1 was detected in immersion fixed iPS2 human induced pluripotent stem cells differentiated to endocrine progenitor cells using Goat Anti-Human Islet‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Islet-1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Islet-1
Met4-Ala349
Accession # P61371
Specificity
Detects human Islet-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Islet-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ISL1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF1837 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Islet-1 Antibody [Unconjugated]

  • insulin gene enhancer protein ISL-1
  • ISL LIM homeobox 1
  • ISL1 transcription factor, LIM/homeodomain
  • ISL1 transcription factor, LIM/homeodomain, (islet-1)
  • ISL1
  • Isl-1
  • Islet1
  • Islet-1

Background

Islet-1 is a member of a family of homeodomain containing transcription factors. It is expressed in all islet cells in the pancreas and is an early marker for motor neuron differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Islet-1 Antibody (AF1837)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 6 applications: ChIP, ICC, IHC, IHC - Paraffin embedded, IHC Not Specified, WB.


Filter By Application
ChIP
(1)
ICC
(2)
IHC
(3)
IHC - Paraffin embedded
(1)
IHC Not Specified
(2)
WB
(1)
All Applications
Filter By Species
Human
(3)
Mouse
(6)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using AF1837 Applications Species
RR Waclaw, LA Ehrman, P Merchan-Sa, V Kohli, D Nardini, K Campbell Foxo1 is a downstream effector of Isl1 in direct pathway striatal projection neuron development within the embryonic mouse telencephalon Mol. Cell. Neurosci, 2017;80(0):44-51. 2017 [PMID: 28213137] (IHC,, Mouse) IHC Mouse
CJ Woo, VK Maier, R Davey, J Brennan, G Li, J Brothers, B Schwartz, S Gordo, A Kasper, TR Okamoto, HE Johansson, B Mandefro, D Sareen, P Bialek, BN Chau, B Bhat, D Bullough, J Barsoum Gene activation of SMN by selective disruption of lncRNA-mediated recruitment of PRC2 for the treatment of spinal muscular atrophy Proc. Natl. Acad. Sci. U.S.A, 2017;114(8):E1509-E1518. 2017 [PMID: 28193854] (ICC,, Human) ICC Human
Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells Sci Rep, 2016;6(0):25474. 2016 [PMID: 27146274] (IHC - Paraffin embedded,, Human) IHC - Paraffin embedded Human
Adams, Katrina, Rousso, David L, Umbach, Joy A, Novitch, Bennett Foxp1-mediated programming of limb-innervating motor neurons from mouse and human embryonic stem cells. Nat Commun, 2015;6(0):6778. 2015 [PMID: 25868900] (IHC Not Specified,, Mouse) IHC Not Specified Mouse
Gu, Zirong, Imai, Fumiyasu, Kim, In Jung, Fujita, Hiroko, Katayama, Kei ichi, Mori, Kensaku, Yoshihara, Yoshihir, Yoshida, Yutaka Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion. PLoS ONE, 2015;10(3):e0121550. 2015 [PMID: 25826454] (IHC Not Specified,, Mouse) IHC Not Specified Mouse
Li, Renzhong, Wu, Fuguo, Ruonala, Raili, Sapkota, Darshan, Hu, Zihua, Mu, Xiuqian Isl1 and Pou4f2 form a complex to regulate target genes in developing retinal ganglion cells. PLoS ONE, 2014;9(3):e92105. 2014 [PMID: 24643061] (ChIP,, Mouse) ChIP Mouse
Chen J, Fu R, Cui Y, Li Y, Pan J, Liu J, Luo H, Yin J, Li D, Cui S LIM-homeodomain transcription factor Isl-1 mediates the effect of leptin on insulin secretion in mice. J Biol Chem, 2013;288(17):12395-405. 2013 [PMID: 23504315] (WB,, Mouse) WB Mouse
Hazen VM, Phan KD, Hudiburgh S, Butler SJ Inhibitory Smads differentially regulate cell fate specification and axon dynamics in the dorsal spinal cord. Dev. Biol., 2011;356(2):566-75. 2011 [PMID: 21718693] (IHC,, Chicken) IHC Chicken
Yau WW, Tang MK, Chen E Cardiogenol C can induce Mouse Hair Bulge Progenitor Cells to Transdifferentiate into Cardiomyocyte-like Cells. Proteome Sci, 2011;9(1):3. 2011 [PMID: 21247432] (ICC,, Mouse) ICC Mouse
Karumbayaram S, Novitch BG, Patterson M, Umbach JA, Richter L, Lindgren A, Conway AE, Clark AT, Goldman SA, Plath K, Wiedau-Pazos M, Kornblum HI, Lowry WE Directed differentiation of human-induced pluripotent stem cells generates active motor neurons. Stem Cells, 2009;27(4):806-11. 2009 [PMID: 19350680] (IHC,, Human) IHC Human

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Bioinformatics

Gene Symbol ISL1
Entrez
Uniprot