Islet-1 Antibody [Unconjugated]

Western blot shows lysates of JOY6 human induced pluripotent stem cells undifferentiated and differentiatied into motor neurons. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Islet‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Islet‑1 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Islet‑1 was detected in immersion fixed iPS2 human induced pluripotent stem cells differentiated to endocrine progenitor cells using Goat Anti-Human Islet‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Islet‑1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human Islet‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in islet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western lane view shows lysates of JOY6 human induced pluripotent stem cells undifferentiated and differentiated into motor neurons, loaded at 0.2 mg/mL. A specific band was detected for Islet‑1 at approximately 53 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Islet‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Islet-1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Islet-1
  Met4-Ala349
  Accession # P61371
• Specificity: Detects human Islet-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Islet-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ISL1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Islet-1 Antibody [Unconjugated]

• insulin gene enhancer protein ISL-1
• ISL LIM homeobox 1
• ISL1 transcription factor, LIM/homeodomain
• ISL1 transcription factor, LIM/homeodomain, (islet-1)
• ISL1
• Isl-1
• Islet1
• Islet-1

Background

Islet-1 is a member of a family of homeodomain containing transcription factors. It is expressed in all islet cells in the pancreas and is an early marker for motor neuron differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Islet-1 Antibody (AF1837)(16)

Publications using AF1837

• S Gupta, R Kawaguchi, E Heinrichs, S Gallardo, S Castellano, I Mandric, BG Novitch, SJ Butler In�vitro atlas of dorsal spinal interneurons reveals Wnt signaling as a critical regulator of progenitor expansion Oncogene, 2022;40(3):111119. 2022 [PMID: 35858555] (IHC, Human)
• M Ohgushi, N Taniyama, A Vandenbon, M Eiraku Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model Cell Reports, 2022;39(12):110973. 2022 [PMID: 35732132] (Flow Cytometry, Human)
• X Zhou, Y Lu, F Zhao, J Dong, W Ma, S Zhong, M Wang, B Wang, Y Zhao, Y Shi, Q Ma, T Lu, J Zhang, X Wang, Q Wu Deciphering the spatial-temporal transcriptional landscape of human hypothalamus development Cell Stem Cell, 2021;0(0):. 2021 [PMID: 34879244] (IHC, Human)
• L Zhang, J Avery, A Yin, AM Singh, TS Cliff, H Yin, S Dalton Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State Cell Stem Cell, 2020;0(0):. 2020 [PMID: 32783886] (IHC, Human)
• L Zhang, J Avery, A Yin, AM Singh, TS Cliff, H Yin, S Dalton Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State Cell Stem Cell, 2020;0(0):. 2020 [PMID: 32783886] (Western Blot, Human)
• ME Frendo, A da Silva, KD Phan, S Riche, SJ Butler The cofilin/Limk1 pathway controls the growth rate of both developing and regenerating motor axons J. Neurosci., 2019;0(0):. 2019 [PMID: 31578231] (IHC, Mouse)
• RR Waclaw, LA Ehrman, P Merchan-Sa, V Kohli, D Nardini, K Campbell Foxo1 is a downstream effector of Isl1 in direct pathway striatal projection neuron development within the embryonic mouse telencephalon Mol. Cell. Neurosci, 2017;80(0):44-51. 2017 [PMID: 28213137] (IHC, Mouse)
• CJ Woo, VK Maier, R Davey, J Brennan, G Li, J Brothers, B Schwartz, S Gordo, A Kasper, TR Okamoto, HE Johansson, B Mandefro, D Sareen, P Bialek, BN Chau, B Bhat, D Bullough, J Barsoum Gene activation of SMN by selective disruption of lncRNA-mediated recruitment of PRC2 for the treatment of spinal muscular atrophy Proc. Natl. Acad. Sci. U.S.A, 2017;114(8):E1509-E1518. 2017 [PMID: 28193854] (ICC, Human)
• Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells Sci Rep, 2016;6(0):25474. 2016 [PMID: 27146274] (IHC-P, Human)
• Adams , Katrina, Rousso , David L, Umbach , Joy A, Novitch , Bennett Foxp1-mediated programming of limb-innervating motor neurons from mouse and human embryonic stem cells. Nat Commun, 2015;6(0):6778. 2015 [PMID: 25868900] (IHC, Mouse)
• Gu , Zirong, Imai , Fumiyasu, Kim , In Jung, Fujita , Hiroko, Katayama , Kei ichi, Mori , Kensaku, Yoshihara , Yoshihir, Yoshida , Yutaka Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion. PLoS ONE, 2015;10(3):e0121550. 2015 [PMID: 25826454] (IHC, Mouse)
• Li , Renzhong, Wu , Fuguo, Ruonala , Raili, Sapkota , Darshan, Hu , Zihua, Mu , Xiuqian Isl1 and Pou4f2 form a complex to regulate target genes in developing retinal ganglion cells. PLoS ONE, 2014;9(3):e92105. 2014 [PMID: 24643061] (ChIP, Mouse)
• Chen J, Fu R, Cui Y, Li Y, Pan J, Liu J, Luo H, Yin J, Li D, Cui S LIM-homeodomain transcription factor Isl-1 mediates the effect of leptin on insulin secretion in mice. J Biol Chem, 2013;288(17):12395-405. 2013 [PMID: 23504315] (Western Blot, Mouse)
• Hazen VM, Phan KD, Hudiburgh S, Butler SJ Inhibitory Smads differentially regulate cell fate specification and axon dynamics in the dorsal spinal cord. Dev. Biol., 2011;356(2):566-75. 2011 [PMID: 21718693] (IHC, Chicken)
• Yau WW, Tang MK, Chen E Cardiogenol C can induce Mouse Hair Bulge Progenitor Cells to Transdifferentiate into Cardiomyocyte-like Cells. Proteome Sci, 2011;9(1):3. 2011 [PMID: 21247432] (ICC, Mouse)
• Karumbayaram S, Novitch BG, Patterson M, Umbach JA, Richter L, Lindgren A, Conway AE, Clark AT, Goldman SA, Plath K, Wiedau-Pazos M, Kornblum HI, Lowry WE Directed differentiation of human-induced pluripotent stem cells generates active motor neurons. Stem Cells, 2009;27(4):806-11. 2009 [PMID: 19350680] (IHC, Human)

Bioinformatics

• Gene Symbol: ISL1
• Uniprot:
  • P61371()