NKX2.2 Antibody

Images

 
Western Blot: NKX2.2 Antibody [NBP1-82554] - Analysis in human cell line U-138 MG.
Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554] - Staining of human cerebellum shows moderate postivity in cells in molecular layer.
Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554] - Staining of human pancreas shows strong nuclear positivity in islet cells.
Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554] - Staining of human cerebral cortex shows strong nuclear postivity in glial cells.
Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, Fe, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NKX2.2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SLTNTKTGFSVKDILDLPDTNDEEGSVAEGPEEENEGPEPAKRAGPLGQGALDAVQSLPLKNPFYDSSDNPYTRWLASTEGLQYSLHGLAAGAPPQDSSSKSPEPSADESPDNDKETP
Predicted Species
Rat (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NKX2-2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockout Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. NKX2.2 antibody validated for IHC-Frozen, ICC/IF from verified customer reviews. KO validated (bioRxiv Jan 1 2020).
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
NKX2.2 Protein (NBP1-82554PEP)
Reviewed Applications
Read 2 Reviews rated 4
using
NBP1-82554 in the following applications:

Publications
Read Publications using
NBP1-82554 in the following applications:

  • IHC
    1 publication
  • KO
    1 publication

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24413736). Feline, Mouse reactivity reported from verified customer reviews.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NKX2.2 Antibody

  • Homeobox protein NK-2 homolog B
  • NK-2 (Drosophila) homolog B
  • NK2 homeobox 2
  • NK2 transcription factor related, locus 2 (Drosophila)
  • NK2 transcription factor related, locus 2
  • NK2 transcription factor-like protein B
  • NKX2.2
  • NKX2.2homeobox protein Nkx-2.2
  • NKX2B
  • NKX2BNK-2 homolog B

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NKX2.2 Antibody (NBP1-82554)(4)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC, KO.


Filter By Application
IHC
(1)
KO
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Reviews for NKX2.2 Antibody (NBP1-82554) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Feline.

Reviews using NBP1-82554:
Filter by Applications
Immunocytochemistry
(1)
Immunohistochemistry-Frozen
(1)
All Applications
Filter by Species
Mouse
(1)
Feline
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry NKX2.2 NBP1-82554
Enlarge
3
reviewed by:
Kazuya Oikawa
Immunocytochemistry Feline 06/23/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedOptic nerve
SpeciesFeline
LotB81867

Comments

Comments4%PFA fixed tissue
Immunohistochemistry-Frozen NKX2.2 NBP1-82554
Enlarge
5
reviewed by:
Verified Customer
Immunohistochemistry-Frozen Mouse 11/09/2016
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse pancreas in OCT
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NKX2.2 Antibody (NBP1-82554) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for NKX2.2 Antibody (NBP1-82554)

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Recent Reviews

4
5
1
4
0
3
1
2
0
1
0

Kazuya Oikawa
06/23/2017
Application: Immunocytochemistry
Species: Feline

Verified Customer
11/09/2016
Application: Immunohistochemistry-Frozen
Species: Mouse

Bioinformatics

Gene Symbol NKX2-2
Entrez
Uniprot