Otx2 Antibody [Unconjugated]

Images

 
Simple Western lane view shows lysates of IMR‑32 human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Otx2 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat ...read more
Otx2 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 10 µg/mL for 3 hours at room ...read more
Western blot shows lysates of IMR‑32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Otx2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Otx2
Met1-Leu289
Accession # P32243
Specificity
Detects human Otx2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
OTX2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF1979 in the following applications:

Publications
Read Publications using
AF1979 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Otx2 Antibody [Unconjugated]

  • homeobox protein OTX2
  • MCOPS5
  • MGC45000
  • orthodenticle homeobox 2
  • orthodenticle homolog 2 (Drosophila)
  • Orthodenticle homolog 2
  • Otx2

Background

Otx2 is a member of the bicoid subfamily of homeodomain-containing transcription factors. It may play a role in brain and sensory organ development (1). Isoform a differs from isoform b by having an eight amino acid residue insertion between P32 and A33 of Otx2b.
  1. Nagao, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3737.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Otx2 Antibody (AF1979)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 7 applications: ICC, IHC, IHC Not Specified, IHC OCT-embedded, IHC Paraffin-embedded, IHC-Fr, WB.


Filter By Application
ICC
(5)
IHC
(2)
IHC Not Specified
(1)
IHC OCT-embedded
(2)
IHC Paraffin-embedded
(2)
IHC-Fr
(1)
WB
(1)
All Applications
Filter By Species
Human
(7)
Mouse
(6)
Chicken
(1)
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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using AF1979 Applications Species
JY Kim, JS Lee, HS Hwang, DR Lee, CY Park, SJ Jung, YR You, DS Kim, DW Kim Wnt signal activation induces midbrain specification through direct binding of the beta-catenin/TCF4 complex to the EN1 promoter in human pluripotent stem cells Exp. Mol. Med., 2018;50(4):24. 2018 [PMID: 29650976] (ICC,, Human) ICC Human
L Todd, L Suarez, C Quinn, AJ Fischer Retinoic Acid-Signaling Regulates the Proliferative and Neurogenic Capacity of M�ller Glia-Derived Progenitor Cells in the Avian Retina Stem Cells, 2017;0(0):. 2017 [PMID: 29193451] (IHC,, Chicken) IHC Chicken
PA Nakamura, AA Shimchuk, S Tang, Z Wang, K DeGolier, S Ding, TA Reh Small molecule Photoregulin3 prevents retinal degeneration in the RhoP23H mouse model of retinitis pigmentosa Elife, 2017;6(0):. 2017 [PMID: 29148976] (IHC-Fr,, Mouse) IHC-Fr Mouse
Activated monocytes resist elimination by retinal pigment epithelium and downregulate their OTX2 expression via TNF-? Aging Cell, 2016;0(0):. 2016 [PMID: 27660103] (WB,, Human) WB Human
Edri, Reuven, Yaffe, Yakey, Ziller, Michael, Mutukula, Naresh, Volkman, Rotem, David, Eyal, Jacob-Hirsch, Jasmine, Malcov, Hagar, Levy, Carmit, Rechavi, Gideon, Gat-Viks, Irit, Meissner, Alexande, Elkabetz, Yechiel Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors. Nat Commun, 2015;6(0):6500. 2015 [PMID: 25799239] (ICC,, Human) ICC Human
Wu Q, Fukuda K, Weinstein M, Graff J, Saga Y SMAD2 and p38 signaling pathways act in concert to determine XY primordial germ cell fate in mice. Development, 2015;142(3):575-86. 2015 [PMID: 25605784] (IHC OCT-embedded,, Mouse) IHC OCT-embedded Mouse
Zou M, Luo H, Xiang M Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord. Dev Dyn, 2015;244(1):86-97. 2015 [PMID: 25179941] (IHC,, Mouse) IHC Mouse
Di Giovannantonio, Luca G, Di Salvio, Michela, Omodei, Daniela, Prakash, Nilima, Wurst, Wolfgang, Pierani, Alessand, Acampora, Dario, Simeone, Antonio Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity. Development, 2014;141(2):377-88. 2014 [PMID: 24335253] (IHC Not Specified,, Mouse) IHC Not Specified Mouse
Stott, Simon R, Metzakopian, Emmanoui, Lin, Wei, Kaestner, Klaus H, Hen, Rene, Ang, Siew-Lan Foxa1 and foxa2 are required for the maintenance of dopaminergic properties in ventral midbrain neurons at late embryonic stages. J Neurosci, 2013;33(18):8022-34. 2013 [PMID: 23637192] (IHC OCT-embedded,, Mouse) IHC OCT-embedded Mouse
Rijlaarsdam MA, van Herk HA, Gillis AJ Specific detection of OCT3/4 isoform A/B/B1 expression in solid (germ cell) tumours and cell lines: confirmation of OCT3/4 specificity for germ cell tumours. Br. J. Cancer, 2011;105(6):854-63. 2011 [PMID: 21847120] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Show All 14 Publications.

Review for Otx2 Antibody (AF1979) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF1979:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Otx2 AF1979
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reviewed by:
Anonymous
ICC Mouse 08/04/2016
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Summary

ApplicationImmunocytochemistry
Sample TestedR1 wild type Mouse embryonic Stem Cells
SpeciesMouse
LotKNO0615111

Blocking

Blocking DetailsBlocking: 0.1% Triton X in PBS with 5% donkey serum, 1 hr at RT

Primary Anitbody

Dilution Ratio1:300, 14 hrs, 4 C, Buffer: 0.1% Triton X + 5% donkey serum in PBS

Details

Detection NotesFluorescence Imaging, AF488 with EGFP filter cube, Nikon Eclipse Ti microscope, Exposure time: 1 sec
Fixation DetailsFixed for 10 mins with 4% formaldehyde at room temperature. Permeablization with 0.2% TritonX in PBS for 15 mins at RT
Wash DescriptionPBS,~1 min, 3 times

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
08/04/2016
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol OTX2
Entrez
Uniprot