Otx2 Antibody [Unconjugated]

Western blot shows lysates of IMR‑32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Otx2 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Otx2 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Otx2 was detected in immersion fixed paraffin-embedded sections of mouse embryo (14 d.p.c.) using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing nervous system. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western lane view shows lysates of IMR‑32 human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Otx2 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Otx2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Otx2
  Met1-Leu289
  Accession # P32243
• Specificity: Detects human Otx2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: OTX2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Otx2 Antibody [Unconjugated]

• homeobox protein OTX2
• MCOPS5
• MGC45000
• orthodenticle homeobox 2
• orthodenticle homolog 2 (Drosophila)
• Orthodenticle homolog 2
• Otx2

Background

Otx2 is a member of the bicoid subfamily of homeodomain-containing transcription factors. It may play a role in brain and sensory organ development (1). Isoform a differs from isoform b by having an eight amino acid residue insertion between P32 and A33 of Otx2b.

1. Nagao, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3737.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Otx2 Antibody (AF1979)(75)

Publications using AF1979

• E Boobalan, AH Thompson, RP Alur, DM McGaughey, L Dong, G Shih, ER Vieta-Ferr, IF Onojafe, VK Kalaskar, G Arno, AJ Lotery, B Guan, C Bender, O Memon, L Brinster, C Soleilhavo, L Panman, TC Badea, A Minella, AJ Lopez, SM Thomasy, A Moshiri, D Blain, RB Hufnagel, T Cogliati, K Bharti, BP Brooks Zfp503/Nlz2 Is Required for RPE Differentiation and Optic Fissure Closure Investigative Ophthalmology & Visual Science, 2022;63(12):5. 2022 [PMID: 36326727] (IHC/IF, Mouse)
• M Langouët, C Jolicoeur, A Javed, P Mattar, MD Gearhart, SP Daiger, M Bertelsen, L Tranebjærg, ND Rendtorff, K Grønskov, C Jespersgaa, R Chen, Z Sun, H Li, N Alirezaie, J Majewski, VJ Bardwell, R Sui, RK Koenekoop, M Cayouette Mutations in BCOR, a co-repressor of CRX/OTX2, are associated with early-onset retinal degeneration Science Advances, 2022;8(36):eabh2868. 2022 [PMID: 36070393] (IHC, Mouse)
• M Alonzo, J Contreras, S Ye, H Lin, L Hernandez-, KL McBride, K Texter, V Garg, MT Zhao Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS) Stem Cell Research, 2022;64(0):102892. 2022 [PMID: 35987121] (Flow Cytometry, Human)
• J Contreras, M Alonzo, S Ye, H Lin, L Hernandez-, KL McBride, K Texter, V Garg, MT Zhao Generation of an induced pluripotent stem cell line NCHi003-A from a 11-year-old male with pulmonary atresia with intact ventricular septum (PA-IVS) Stem Cell Research, 2022;64(0):102893. 2022 [PMID: 35987120] (Flow Cytometry, Human)
• J Liu, GC Black, SJ Kimber, PI Sergouniot Generation of a human induced pluripotent stem cell line carrying the TYR c.575C>A (p.Ser192Tyr) and c.1205G>A (p.Arg402Gln) variants in homozygous state using CRISPR-Cas9 genome editing Stem Cell Research, 2022;64(0):102880. 2022 [PMID: 35933957] (Flow Cytometry, Human)
• J Lotila, T Hyvärinen, H Skottman, L Airas, S Narkilahti, S Hagman Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient Oncogene, 2022;63(0):102865. 2022 [PMID: 35843021] (Flow Cytometry, Human)
• RR Khadim, RK Vadivelu, T Utami, FG Torizal, M Nishikawa, Y Sakai Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs International Journal of Molecular Sciences, 2022;23(13):. 2022 [PMID: 35806277] (ICC, Human)
• IC Han, LR Bohrer, KN Gibson-Cor, LA Wiley, A Shrestha, BE Harman, C Jiao, EH Sohn, R Wendland, BN Allen, KS Worthingto, RF Mullins, EM Stone, BA Tucker Biocompatibility of Human Induced Pluripotent Stem Cell-Derived Retinal Progenitor Cell Grafts in Immunocompromised Rats Cell Transplantation, 2022;31(0):9636897221104. 2022 [PMID: 35758274] (ICC, Human)
• HM Lee, AH Kim, S Hwang, J Jung, H Seol, JJ Sung, SM Jeong, YM Choi, JK Jun, HS Kim, J Jang Generation of alphaMHC-EGFP knock-in in human pluripotent stem cell line, SNUe003-A-3 using CRISPR/Cas9-based gene targeting Stem Cell Research, 2022;61(0):102779. 2022 [PMID: 35427904] (ICC, Human)
• A Manhas, JWS Jahng, CD Vera, SP Shenoy, JW Knowles, JC Wu Generation of two iPSC lines from hypertrophic cardiomyopathy patients carrying MYBPC3 and PRKAG2 variants Stem Cell Research, 2022;61(0):102774. 2022 [PMID: 35413566] (ICC, Human)
• G Iannello, A Patel, D Sirabella, B Corneo, VV Thaker Derivation and characterization of the induced pluripotent stem cell line CUIMCi004-A from a patient with a novel frameshift variant in exon 18a of OCRL Stem Cell Research, 2021;59(0):102635. 2021 [PMID: 35074682] (IHC, Human)
• S Touhami, F Béguier, T Yang, S Augustin, C Roubeix, F Blond, JB Conart, JA Sahel, B Bodaghi, C Delarasse, X Guillonnea, F Sennlaub Hypoxia Inhibits Subretinal Inflammation Resolution Thrombospondin-1 Dependently International Journal of Molecular Sciences, 2022;23(2):. 2022 [PMID: 35054863] (ICC, Human)
• P Nouri, A Zimmer, S Brüggemann, R Friedrich, R Kühn, N Prakash Generation of a NES-mScarlet Red Fluorescent Reporter Human iPSC Line for Live Cell Imaging and Flow Cytometric Analysis and Sorting Using CRISPR-Cas9-Mediated Gene Editing Cells, 2022;11(2):. 2022 [PMID: 35053384] (Western Blot, Human)
• P Aldrin-Kir, M Åkerblom, T Cardoso, S Nolbrant, AF Adler, X Liu, A Heuer, M Davidsson, M Parmar, T Björklund A novel two-factor monosynaptic TRIO tracing method for assessment of circuit integration of hESC-derived dopamine transplants Stem Cell Reports, 2021;17(1):159-172. 2021 [PMID: 34971563] (ICC, Human)
• C Lee, S Cho, C Lai, S Shenoy, R Vagelos, JC Wu Generation of three iPSC lines from dilated cardiomyopathy patients carrying a pathogenic LMNA variant Stem Cell Research, 2021;59(0):102638. 2021 [PMID: 34954454] (Flow Cytometry, Human)
• J Langkabel, A Horne, L Bonaguro, L Holsten, T Hesse, A Knaus, Y Riedel, M Becker, K Händler, T Elmzzahi, K Bassler, N Reusch, LH Yeghiazari, T Pecht, A Saglam, T Ulas, AC Aschenbren, F Kaiser, C Kubaczka, JL Schultze, H Schorle Induction of Rosette-to-Lumen stage embryoids using reprogramming paradigms in ESCs Nature Communications, 2021;12(1):7322. 2021 [PMID: 34916498] (ICC, Mouse)
• N Belbachir, C Lai, JW Rhee, Y Zhuge, MV Perez, K Sallam, JC Wu Generation of two induced pluripotent stem cell lines from Brugada syndrome affected patients carrying SCN5A mutations Stem Cell Research, 2021;57(0):102605. 2021 [PMID: 34856468] (Flow Cytometry, Human)
• S Lim, YJ Kim, S Park, JH Choi, Y Sung, K Nishimori, Z Kozmik, HW Lee, JW Kim mTORC1-induced retinal progenitor cell overproliferation leads to accelerated mitotic aging and degeneration of descendent M�ller glia Elife, 2021;10(0):. 2021 [PMID: 34677125] (IHC, Mouse)
• L Zilova, V Weinhardt, T Tavhelidse, C Schlagheck, T Thumberger, J Wittbrodt Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development Elife, 2021;10(0):. 2021 [PMID: 34252023] (IHC, Medaka)
• G Iannello, A Patel, D Sirabella, B Corneo, V Thaker Generation of the iPSC line CUIMCi003-A derived from a patient with severe early onset obesity Stem Cell Research, 2021;54(0):102432. 2021 [PMID: 34214899] (ICC, Human)
• D Meisterman, A Bruneau, S Loubersac, A Reignier, J Firmin, V François-C, S Kilens, Y Lelièvre, J Lammers, M Feyeux, P Hulin, S Nedellec, B Bretin, G Castel, N Allègre, S Covin, A Bihouée, M Soumillon, T Mikkelsen, P Barrière, C Chazaud, J Chappell, V Pasque, J Bourdon, T Fréour, L David Integrated pseudotime analysis of human pre-implantation embryo single-cell transcriptomes reveals the dynamics of lineage specification Cell Stem Cell, 2021;0(0):. 2021 [PMID: 34004179] (IHC, Human)
• A Yanagida, D Spindlow, J Nichols, A Dattani, A Smith, G Guo Naive stem cell blastocyst model captures human embryo lineage segregation Cell Stem Cell, 2021;28(6):1016-1022.e4. 2021 [PMID: 33957081] (ICC, Human)
• L Liu, SP Shenoy, JWS Jahng, Y Liu, JW Knowles, Y Zhuge, JC Wu Generation of two heterozygous MYBPC3 mutation-carrying human iPSC lines, SCVIi001-A and SCVIi002-A, for modeling hypertrophic cardiomyopathy Stem Cell Research, 2021;53(0):102279. 2021 [PMID: 33743363] (Flow Cytometry, Human)
• MA Molè, A Weberling, R Fässler, A Campbell, S Fishel, M Zernicka-G Integrin &beta1 coordinates survival and morphogenesis of the embryonic lineage upon implantation and pluripotency transition Cell Reports, 2021;34(10):108834. 2021 [PMID: 33691117] (IHC, Mouse)
• X Wang, Y Xiang, Y Yu, R Wang, Y Zhang, Q Xu, H Sun, ZA Zhao, X Jiang, X Wang, X Lu, D Qin, Y Quan, J Zhang, N Shyh-Chang, H Wang, N Jing, W Xie, L Li Formative pluripotent stem cells show features of epiblast cells poised for gastrulation Cell Research, 2021;0(0):. 2021 [PMID: 33608671] (Western Blot, Mouse)
• A Weberling, M Zernicka-G Trophectoderm mechanics direct epiblast shape upon embryo implantation Cell Reports, 2021;34(3):108655. 2021 [PMID: 33472064] (IHC, Mouse)
• F Nilsson, P Storm, E Sozzi, D Hidalgo Gi, M Birtele, Y Sharma, M Parmar, A Fiorenzano Single-Cell Profiling of Coding and Noncoding Genes in Human Dopamine Neuron Differentiation Cells, 2021;10(1):. 2021 [PMID: 33445654] (ICC, Human)
• H De Belly, A Stubb, A Yanagida, C Labouesse, PH Jones, EK Paluch, KJ Chalut Membrane Tension Gates ERK-Mediated Regulation of Pluripotent Cell Fate Cell Stem Cell, 2020;0(0):. 2020 [PMID: 33217323] (IHC, Mouse)
• T Malankhano, L Suldina, E Grigor'eva, S Medvedev, J Minina, K Morozova, E Kiseleva, S Zakian, A Malakhova A Human Induced Pluripotent Stem Cell-Derived Isogenic Model of Huntington's Disease Based on Neuronal Cells Has Several Relevant Phenotypic Abnormalities J Pers Med, 2020;10(4):. 2020 [PMID: 33182269] (ICC, Human)
• F Jacob, SR Pather, WK Huang, F Zhang, SZH Wong, H Zhou, B Cubitt, W Fan, CZ Chen, M Xu, M Pradhan, DY Zhang, W Zheng, AG Bang, H Song, J Carlos de, GL Ming Human Pluripotent Stem Cell-Derived Neural Cells and Brain Organoids Reveal SARS-CoV-2 Neurotropism Predominates in Choroid Plexus Epithelium Cell Stem Cell, 2020;0(0):. 2020 [PMID: 33010822] (IHC, Human)
• PA Barbuti, BFR Santos, CM Dording, G Cruciani, F Massart, A Hummel, R Krüger Generation of two iPS cell lines (HIHDNDi001-A and HIHDNDi001-B) from a Parkinson�s disease patient carrying the heterozygous p.A30P mutation in SNCA Stem Cell Res, 2020;48(0):101951. 2020 [PMID: 32798915] (ICC, Human)
• PA Barbuti, BFR Santos, CM Dording, G Cruciani, F Massart, A Hummel, R Krüger Generation of two iPS cell lines (HIHDNDi001-A and HIHDNDi001-B) from a Parkinson�s disease patient carrying the heterozygous p.A30P mutation in SNCA Stem Cell Res, 2020;48(0):101951. 2020 [PMID: 32798915] (ICC, Human)
• LS VandenBosc, SG Wohl, MS Wilken, M Hooper, C Finkbeiner, K Cox, L Chipman, TA Reh Developmental changes in the accessible chromatin, transcriptome and Ascl1-binding correlate with the loss in M�ller Glial regenerative potential Sci Rep, 2020;10(1):13615. 2020 [PMID: 32788677] (IHC, Mouse)
• LTP Khoa, YC Tsan, F Mao, DM Kremer, P Sajjakulnu, L Zhang, B Zhou, X Tong, NV Bhanu, C Choudhary, BA Garcia, L Yin, GD Smith, TL Saunders, SL Bielas, CA Lyssiotis, Y Dou Histone Acetyltransferase MOF Blocks Acquisition of Quiescence in Ground-State ESCs through Activating Fatty Acid Oxidation Cell Stem Cell, 2020;0(0):. 2020 [PMID: 32610040] (IHC, Mouse)
• C Kyprianou, N Christodou, RS Hamilton, W Nahaboo, DS Boomgaard, G Amadei, I Migeotte, M Zernicka-G Basement membrane remodelling regulates mouse embryogenesis Nature, 2020;582(7811):253-258. 2020 [PMID: 32523119] (IHC, Mouse)
• N Micali, SK Kim, M Diaz-Busta, G Stein-O'Br, S Seo, JH Shin, BG Rash, S Ma, Y Wang, NA Olivares, JI Arellano, KR Maynard, EJ Fertig, AJ Cross, RW Bürli, NJ Brandon, DR Weinberger, JG Chenoweth, DJ Hoeppner, N Sestan, P Rakic, C Colantuoni, RD McKay Variation of Human Neural Stem Cells Generating Organizer States In�Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates Cell Rep, 2020;31(5):107599. 2020 [PMID: 32375049] (IF, Human)
• R Kitajima, R Nakai, T Imamura, T Kameda, D Kozuka, H Hirai, H Ito, H Imai, M Imamura Modeling of early neural development in vitro by direct neurosphere formation culture of chimpanzee induced pluripotent stem cells Stem Cell Res, 2020;44(0):101749. 2020 [PMID: 32151953] (ICC, Pan troglodytes (Chimpanzee))
• M Bistoletti, G Micheloni, N Baranzini, A Bosi, A Conti, V Filpa, C Pirrone, G Millefanti, E Moro, A Grimaldi, R Valli, A Baj, F Crema, C Giaroni, G Porta Homeoprotein OTX1 and OTX2 involvement in rat myenteric neuron adaptation after DNBS-induced colitis PeerJ, 2020;8(0):e8442. 2020 [PMID: 32095330] (Western Blot, Rat)
• CW Gantner, IR de Luzy, JA Kauhausen, N Moriarty, JC Niclis, CR Bye, V Penna, CPJ Hunt, CM Ermine, CW Pouton, D Kirik, LH Thompson, CL Parish Viral Delivery of GDNF Promotes Functional Integration of Human Stem Cell Grafts in Parkinson's Disease Cell Stem Cell, 2020;0(0):. 2020 [PMID: 32059808] (ICC, Mouse)
• SH Chung, W Shen, KC Davidson, A Pébay, RCB Wong, B Yau, M Gillies Differentiation of Retinal Glial Cells From Human Embryonic Stem Cells by Promoting the Notch Signaling Pathway Front Cell Neurosci, 2019;13(0):527. 2019 [PMID: 31849614] (ICC, Human)
• C Lu, NE Sanjana Generation of a knock-in MAP2-tdTomato reporter human embryonic stem cell line with inducible expression of NEUROG2/1 (NYGCe001-A) Stem Cell Res, 2019;41(0):101643. 2019 [PMID: 31707212] (ICC, Human)
• AJ Duarte, D Ribeiro, R Santos, L Moreira, J Bragança, O Amaral Induced pluripotent stem cell line (INSAi001-A) from a Gaucher disease type 3 patient compound heterozygote for mutations in the GBA1 gene Stem Cell Res, 2019;41(0):101595. 2019 [PMID: 31678773] (ICC, Human)
• MT De Angelis, G Santamaria, EI Parrotta, S Scalise, M Lo Conte, S Gasparini, E Ferlazzo, U Aguglia, C Ciampi, A Sgura, G Cuda Establishment and characterization of induced pluripotent stem cells (iPSCs) from central nervous system lupus erythematosus J. Cell. Mol. Med., 2019;0(0):. 2019 [PMID: 31536674] (ICC, Human)
• J Domingo-Pr, V Abad-Moral, M Riera, R Navarro, B Corcostegu, E Pomares Generation of an induced pluripotent stem cell line (FRIMOi007-A) derived from an incomplete achromatopsia patient carrying a novel homozygous mutation in PDE6C gene Stem Cell Res, 2019;40(0):101569. 2019 [PMID: 31520890] (ICC, Human)
• J Domingo-Pr, M Riera, V Abad-Moral, S Ruiz-Nogal, B Corcostegu, E Pomares Generation of Best disease-derived induced pluripotent stem cell line (FRIMOi006-A) carrying a novel dominant mutation in BEST1 gene Stem Cell Res, 2019;40(0):101570. 2019 [PMID: 31518904] (ICC, Human)
• J Domingo-Pr, M Riera, A Burés-Jels, B Corcostegu, E Pomares Establishment of an induced pluripotent stem cell line (FRIMOi005-A) derived from a retinitis pigmentosa patient carrying a dominant mutation in RHO gene Stem Cell Res, 2019;38(0):101468. 2019 [PMID: 31146251] (ICC, Human)
• M Riera, A Patel, A Burés-Jels, B Corcostegu, S Chang, E Pomares, B Corneo, JR Sparrow Generation of two iPS cell lines (FRIMOi003-A and FRIMOi004-A) derived from Stargardt patients carrying ABCA4 compound heterozygous mutations Stem Cell Res, 2019;36(0):101389. 2019 [PMID: 30798147] (ICC, Human)
• M Riera, A Patel, B Corcostegu, S Chang, B Corneo, JR Sparrow, E Pomares Generation of an induced pluripotent stem cell line (FRIMOi002-A) from a retinitis pigmentosa patient carrying compound heterozygous mutations in USH2A gene Stem Cell Res, 2019;35(0):101386. 2019 [PMID: 30685615] (ICC, Human)
• M Riera, A Patel, B Corcostegu, S Chang, JR Sparrow, E Pomares, B Corneo Establishment and characterization of an iPSC line (FRIMOi001-A) derived from a retinitis pigmentosa patient carrying PDE6A mutations Stem Cell Res, 2019;35(0):101385. 2019 [PMID: 30685614] (ICC, Human)
• Y Chen, J Yang, H Geng, L Li, J Li, B Cheng, X Ma, H Li, L Hou Photoreceptor degeneration in microphthalmia (Mitf) mice: partial rescue by pigment epithelium-derived factor Dis Model Mech, 2019;12(1):. 2019 [PMID: 30651300] (IHC-Fr, Mouse)
• KE Worley, AS Chin, LQ Wan Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation Stem Cells Int, 2018;2018(0):1848605. 2018 [PMID: 30627170] (ICC, Human)
• JA Maguire, AL Gagne, P Gonzalez-A, BL Davidson, V Shakkottai, P Gadue, DL French Generation of Spinocerebellar Ataxia Type 2 induced pluripotent stem cell lines, CHOPi002-A and CHOPi003-A, from patients with abnormal CAG repeats in the coding region of the ATXN2 gene Stem Cell Res, 2018;34(0):101361. 2018 [PMID: 30611021] (ICC, Human)
• N Ruiz-Reig, M Rakotobe, I Bethus, G Le Menn, HI Huditz, H Marie, T Lamonerie, F D'Autréaux Developmental Requirement of Homeoprotein Otx2 for Specific Habenulo-Interpeduncular Subcircuits J. Neurosci., 2019;39(6):1005-1019. 2019 [PMID: 30593496] (IHC, Mouse)
• Baulier E, Garcia Diaz A, Corneo B, Farber D Generation of a human Ocular Albinism type 1 iPSC line, SEIi001-A, with a mutation in GPR143. Stem Cell Res, 0;33(0):274-277. 0 [PMID: 30513407] (Bioassay, Human)
• R Diacou, Y Zhao, D Zheng, A Cvekl, W Liu Six3 and Six6 Are Jointly Required for the Maintenance of Multipotent Retinal Progenitors through Both Positive and Negative Regulation Cell Rep, 2018;25(9):2510-2523.e4. 2018 [PMID: 30485816] (IHC-Fr, Mouse)
• S Kaur, AG Abu-Shahba, RO Paananen, H Hongisto, H Hiidenmaa, H Skottman, R Seppänen-K, B Mannerströ Small non-coding RNA landscape of extracellular vesicles from human stem cells Sci Rep, 2018;8(1):15503. 2018 [PMID: 30341351] (Differentiation, Human)
• AD Senft, I Costello, HW King, AW Mould, EK Bikoff, EJ Robertson Combinatorial Smad2/3 Activities Downstream of Nodal Signaling Maintain Embryonic/Extra-Embryonic Cell Identities during Lineage Priming Cell Rep, 2018;24(8):1977-1985.e7. 2018 [PMID: 30134160] (ICC, Mouse)
• AL Gagne, JA Maguire, S Gandre-Bab, ST Chou, SK Tasian, ML Loh, MJ Weiss, P Gadue, DL French Generation of a human Juvenile myelomonocytic leukemia iPSC line, CHOPi001-A, with a mutation in CBL Stem Cell Res, 2018;31(0):157-160. 2018 [PMID: 30096712] (ICC, Human)
• SE Harrison, B Sozen, M Zernicka-G In vitro generation of mouse polarized embryo-like structures from embryonic and trophoblast stem cells Nat Protoc, 2018;0(0):. 2018 [PMID: 29988106] (ICC, Mouse)
• JY Kim, JS Lee, HS Hwang, DR Lee, CY Park, SJ Jung, YR You, DS Kim, DW Kim Wnt signal activation induces midbrain specification through direct binding of the beta-catenin/TCF4 complex to the EN1 promoter in human pluripotent stem cells Exp. Mol. Med., 2018;50(4):24. 2018 [PMID: 29650976] (ICC, Human)
• S El Nagar, F Zindy, C Moens, L Martin, D Plassard, MF Roussel, T Lamonerie, N Billon A new genetically engineered mouse model of choroid plexus carcinoma Biochem. Biophys. Res. Commun., 2018;0(0):. 2018 [PMID: 29339161] (IHC, Mouse)
• X Tang, L Jiao, M Zheng, Y Yan, Q Nie, T Wu, X Wan, G Zhang, Y Li, S Wu, B Jiang, H Cai, P Xu, J Duan, X Lin Tau Deficiency Down-Regulated Transcription Factor Orthodenticle Homeobox 2 Expression in the Dopaminergic Neurons in Ventral Tegmental Area and Caused No Obvious Motor Deficits in Mice Neuroscience, 2018;0(0):. 2018 [PMID: 29337233] (IHC, Mouse)
• L Todd, L Suarez, C Quinn, AJ Fischer Retinoic Acid-Signaling Regulates the Proliferative and Neurogenic Capacity of M�ller Glia-Derived Progenitor Cells in the Avian Retina Stem Cells, 2017;0(0):. 2017 [PMID: 29193451] (IHC, Chicken)
• PA Nakamura, AA Shimchuk, S Tang, Z Wang, K DeGolier, S Ding, TA Reh Small molecule Photoregulin3 prevents retinal degeneration in the RhoP23H mouse model of retinitis pigmentosa Elife, 2017;6(0):. 2017 [PMID: 29148976] (IHC-Fr, Mouse)
• Activated monocytes resist elimination by retinal pigment epithelium and downregulate their OTX2 expression via TNF-? Aging Cell, 2016;0(0):. 2016 [PMID: 27660103] (Western Blot, Human)
• Edri , Reuven, Yaffe , Yakey, Ziller , Michael, Mutukula , Naresh, Volkman , Rotem, David , Eyal, Jacob-Hirsch , Jasmine, Malcov , Hagar, Levy , Carmit, Rechavi , Gideon, Gat-Viks , Irit, Meissner , Alexande, Elkabetz , Yechiel Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors. Nat Commun, 2015;6(0):6500. 2015 [PMID: 25799239] (ICC, Human)
• Wu Q, Fukuda K, Weinstein M, Graff J, Saga Y SMAD2 and p38 signaling pathways act in concert to determine XY primordial germ cell fate in mice. Development, 2015;142(3):575-86. 2015 [PMID: 25605784] (IHC, Mouse)
• Zou M, Luo H, Xiang M Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord. Dev Dyn, 2015;244(1):86-97. 2015 [PMID: 25179941] (IHC, Mouse)
• Di Giovannantonio , Luca G, Di Salvio , Michela, Omodei , Daniela, Prakash , Nilima, Wurst , Wolfgang, Pierani , Alessand, Acampora , Dario, Simeone , Antonio Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity. Development, 2014;141(2):377-88. 2014 [PMID: 24335253] (IHC, Mouse)
• Stott , Simon R, Metzakopian , Emmanoui, Lin , Wei, Kaestner , Klaus H, Hen , Rene, Ang , Siew-Lan Foxa1 and foxa2 are required for the maintenance of dopaminergic properties in ventral midbrain neurons at late embryonic stages. J Neurosci, 2013;33(18):8022-34. 2013 [PMID: 23637192] (IHC, Mouse)
• Rijlaarsdam MA, van Herk HA, Gillis AJ Specific detection of OCT3/4 isoform A/B/B1 expression in solid (germ cell) tumours and cell lines: confirmation of OCT3/4 specificity for germ cell tumours. Br. J. Cancer, 2011;105(6):854-63. 2011 [PMID: 21847120] (IHC-P, Human)
• Di Salvio M, Di Giovannantonio LG, Acampora D Otx2 controls neuron subtype identity in ventral tegmental area and antagonizes vulnerability to MPTP. Nat. Neurosci., 2010;13(12):1481-8. 2010 [PMID: 21057506] (IHC-P, Mouse)
• Zeng H, Guo M, Martins-Taylor K Specification of region-specific neurons including forebrain glutamatergic neurons from human induced pluripotent stem cells. PLoS ONE, 2010;5(7):e11853. 2010 [PMID: 20686615] (ICC, Human)
• Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells. FEBS Lett., 2009;583(3):561-7. 2009 [PMID: 19162023] (ICC, Human)
• Pankratz MT, Li XJ, Lavaute TM, Lyons EA, Chen X, Zhang SC Directed neural differentiation of human embryonic stem cells via an obligated primitive anterior stage. Stem Cells, 2007;25(6):1511-20. 2007 [PMID: 17332508] (ICC, Human)

Reviews for Otx2 Antibody (AF1979) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, pleurodeles waltl.

reviewed by: Zeyu Yao ICC pleurodeles waltl 03/30/2020

Summary

• Application: Immunocytochemistry
• Sample Tested: brain
• Species: pleurodeles waltl
• Lot: KNO0919021

reviewed by: Simon Hastreiter ICC Mouse 08/04/2016

Summary

• Application: Immunocytochemistry
• Sample Tested: R1 wild type Mouse embryonic Stem Cells
• Species: Mouse
• Lot: KNO0615111

Bioinformatics

• Gene Symbol: OTX2
• Uniprot:
  • P32243()