Otx2 Antibody [Unconjugated]

Images

 
Simple Western lane view shows lysates of IMR‑32 human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Otx2 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat ...read more
Western blot shows lysates of IMR‑32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by ...read more
Otx2 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 10 µg/mL for 3 hours at room ...read more
Otx2 was detected in immersion fixed paraffin-embedded sections of mouse embryo (14 d.p.c.) using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 1 µg/mL for 1 hour at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Otx2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Otx2
Met1-Leu289
Accession # P32243
Specificity
Detects human Otx2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
OTX2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Control
Otx2 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
AF1979 in the following applications:

Publications
Read Publications using
AF1979 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Otx2 Antibody [Unconjugated]

  • homeobox protein OTX2
  • MCOPS5
  • MGC45000
  • orthodenticle homeobox 2
  • orthodenticle homolog 2 (Drosophila)
  • Orthodenticle homolog 2
  • Otx2

Background

Otx2 is a member of the bicoid subfamily of homeodomain-containing transcription factors. It may play a role in brain and sensory organ development (1). Isoform a differs from isoform b by having an eight amino acid residue insertion between P32 and A33 of Otx2b.
  1. Nagao, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3737.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00005013-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, RNAi
NB100-55393
Species: Hu, Mu
Applications: WB, IHC, IHC-P, PEP-ELISA
NB300-750
Species: Hu, Mu, Rt, Fi, Mk, Pm, Sh
Applications: WB, ICC/IF, IHC, IP
MAB2018
Species: Hu, Mu, Rt
Applications: WB, Simple Western, CyTOF-reported, ICC, ICFlow
NBP1-51575
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
H00001406-M02
Species: Hu, Mu
Applications: WB, ChIP, ELISA, ICC/IF
H00005076-M01
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-89090
Species: Hu
Applications: IHC, IHC-P
H00030062-M02
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB100-56561
Species: Hu, Mu, Rt
Applications: WB
NBP2-01926
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF2400
Species: Hu
Applications: WB, IHC, ChIP, ICC
NBP2-57757
Species: Hu
Applications: IHC, IHC-P
NBP1-84476
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-56853
Species: Hu
Applications: WB, ICC/IF, IHC
NB100-2814
Species: Rt
Applications: WB, ICC/IF, PEP-ELISA
AF1979
Species: Hu
Applications: WB, Simple Western, IHC, ICC/IF

Publications for Otx2 Antibody (AF1979)(34)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 7 applications: Bioassay, Differentiation, ICC, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
Bioassay
(1)
Differentiation
(1)
ICC
(18)
IHC
(8)
IHC-Fr
(3)
IHC-P
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(20)
Mouse
(13)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 34. Show All 34 Publications.
Publications using AF1979 Applications Species
SH Chung, W Shen, KC Davidson, A Pébay, RCB Wong, B Yau, M Gillies Differentiation of Retinal Glial Cells From Human Embryonic Stem Cells by Promoting the Notch Signaling Pathway Front Cell Neurosci, 2019;13(0):527. 2019 [PMID: 31849614] (ICC, Human) ICC Human
MT De Angelis, G Santamaria, EI Parrotta, S Scalise, M Lo Conte, S Gasparini, E Ferlazzo, U Aguglia, C Ciampi, A Sgura, G Cuda Establishment and characterization of induced pluripotent stem cells (iPSCs) from central nervous system lupus erythematosus J. Cell. Mol. Med., 2019;0(0):. 2019 [PMID: 31536674] (ICC, Human) ICC Human
J Domingo-Pr, V Abad-Moral, M Riera, R Navarro, B Corcostegu, E Pomares Generation of an induced pluripotent stem cell line (FRIMOi007-A) derived from an incomplete achromatopsia patient carrying a novel homozygous mutation in PDE6C gene Stem Cell Res, 2019;40(0):101569. 2019 [PMID: 31520890] (ICC, Human) ICC Human
J Domingo-Pr, M Riera, V Abad-Moral, S Ruiz-Nogal, B Corcostegu, E Pomares Generation of Best disease-derived induced pluripotent stem cell line (FRIMOi006-A) carrying a novel dominant mutation in BEST1 gene Stem Cell Res, 2019;40(0):101570. 2019 [PMID: 31518904] (ICC, Human) ICC Human
J Domingo-Pr, M Riera, A Burés-Jels, B Corcostegu, E Pomares Establishment of an induced pluripotent stem cell line (FRIMOi005-A) derived from a retinitis pigmentosa patient carrying a dominant mutation in RHO gene Stem Cell Res, 2019;38(0):101468. 2019 [PMID: 31146251] (ICC, Human) ICC Human
M Riera, A Patel, A Burés-Jels, B Corcostegu, S Chang, E Pomares, B Corneo, JR Sparrow Generation of two iPS cell lines (FRIMOi003-A and FRIMOi004-A) derived from Stargardt patients carrying ABCA4 compound heterozygous mutations Stem Cell Res, 2019;36(0):101389. 2019 [PMID: 30798147] (ICC, Human) ICC Human
M Riera, A Patel, B Corcostegu, S Chang, B Corneo, JR Sparrow, E Pomares Generation of an induced pluripotent stem cell line (FRIMOi002-A) from a retinitis pigmentosa patient carrying compound heterozygous mutations in USH2A gene Stem Cell Res, 2019;35(0):101386. 2019 [PMID: 30685615] (ICC, Human) ICC Human
M Riera, A Patel, B Corcostegu, S Chang, JR Sparrow, E Pomares, B Corneo Establishment and characterization of an iPSC line (FRIMOi001-A) derived from a retinitis pigmentosa patient carrying PDE6A mutations Stem Cell Res, 2019;35(0):101385. 2019 [PMID: 30685614] (ICC, Human) ICC Human
Y Chen, J Yang, H Geng, L Li, J Li, B Cheng, X Ma, H Li, L Hou Photoreceptor degeneration in microphthalmia (Mitf) mice: partial rescue by pigment epithelium-derived factor Dis Model Mech, 2019;12(1):. 2019 [PMID: 30651300] (IHC-Fr, Mouse) IHC-Fr Mouse
KE Worley, AS Chin, LQ Wan Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation Stem Cells Int, 2018;2018(0):1848605. 2018 [PMID: 30627170] (ICC, Human) ICC Human
Show All 34 Publications.

Review for Otx2 Antibody (AF1979) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF1979:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Otx2 AF1979
Enlarge
reviewed by:
Anonymous
ICC Mouse 08/04/2016
View

Summary

ApplicationImmunocytochemistry
Sample TestedR1 wild type Mouse embryonic Stem Cells
SpeciesMouse
LotKNO0615111

Blocking

Blocking DetailsBlocking: 0.1% Triton X in PBS with 5% donkey serum, 1 hr at RT

Primary Anitbody

Dilution Ratio1:300, 14 hrs, 4 C, Buffer: 0.1% Triton X + 5% donkey serum in PBS

Details

Detection NotesFluorescence Imaging, AF488 with EGFP filter cube, Nikon Eclipse Ti microscope, Exposure time: 1 sec
Fixation DetailsFixed for 10 mins with 4% formaldehyde at room temperature. Permeablization with 0.2% TritonX in PBS for 15 mins at RT
Wash DescriptionPBS,~1 min, 3 times

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Otx2 Antibody (AF1979) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF1979

Additional Otx2 Products

Bioinformatics Tool for Otx2 Antibody (AF1979)

Discover related pathways, diseases and genes to Otx2 Antibody (AF1979). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Otx2 Antibody (AF1979)

Discover more about diseases related to Otx2 Antibody (AF1979).
 

Pathways for Otx2 Antibody (AF1979)

View related products by pathway.

PTMs for Otx2 Antibody (AF1979)

Learn more about PTMs related to Otx2 Antibody (AF1979).

Blogs on Otx2

There are no specific blogs for Otx2, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
08/04/2016
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol OTX2
Uniprot
Novus 100% Guarantee