Otx2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of IMR‑32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by ...read more
Otx2 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 10 µg/mL for 3 hours at room ...read more
Otx2 was detected in immersion fixed paraffin-embedded sections of mouse embryo (14 d.p.c.) using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 1 µg/mL for 1 hour at ...read more
Simple Western lane view shows lysates of IMR‑32 human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Otx2 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat ...read more
Generation and characterization of systemic lupus erythematosus (SLE)‐specific human‐induced pluripotent stem cells (hiPSCs). (A) Dermal fibroblasts derived from patient with SLE were reprogrammed into iPSCs using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Otx2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Otx2
Met1-Leu289
Accession # P32243
Specificity
Detects human Otx2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
OTX2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF1979 in the following applications:

Publications
Read Publications using
AF1979 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Otx2 Antibody [Unconjugated]

  • homeobox protein OTX2
  • MCOPS5
  • MGC45000
  • orthodenticle homeobox 2
  • orthodenticle homolog 2 (Drosophila)
  • Orthodenticle homolog 2
  • Otx2

Background

Otx2 is a member of the bicoid subfamily of homeodomain-containing transcription factors. It may play a role in brain and sensory organ development (1). Isoform a differs from isoform b by having an eight amino acid residue insertion between P32 and A33 of Otx2b.
  1. Nagao, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3737.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Otx2 Antibody (AF1979)(147)

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Chicken, Medaka, Pan troglodytes (Chimpanzee), Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Transgenic Mouse, Zebrafish.

We have publications tested in 11 applications: Bioassay, Cell Culture, Differentiation, Flow Cytometry, ICC, IF, IHC, IHC-Fr, IHC-P, IHC/IF, Western Blot.


Filter By Application
Bioassay
(1)
Cell Culture
(1)
Differentiation
(2)
Flow Cytometry
(11)
ICC
(54)
IF
(1)
IHC
(27)
IHC-Fr
(4)
IHC-P
(2)
IHC/IF
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(55)
Mouse
(30)
Rat
(2)
Chicken
(1)
Medaka
(1)
Pan troglodytes (Chimpanzee)
(1)
Porcine
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Transgenic Mouse
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 147. Show All 147 Publications.
Publications using AF1979 Applications Species
Driver, K;Vo, C;Scriba, CK;Saker, S;Larmonier, T;Malfatti, E;Romero, NB;Ravenscroft, G;Laing, NG;Taylor, RL;Clayton, JS; Generation of two induced pluripotent stem cell lines from a 33-year-old central core disease patient with a heterozygous dominant c.14145_14156delCTACTGGGACA (p.Asn4715_Asp4718del) deletion in the RYR1 gene Stem cell research 2023-11-22 [PMID: 38029555] (ICC, Human) ICC Human
Tao, Y;Li, X;Dong, Q;Kong, L;Petersen, AJ;Yan, Y;Xu, K;Zima, S;Li, Y;Schmidt, DK;Ayala, M;Mathivanan, S;Sousa, AMM;Chang, Q;Zhang, SC; Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells Nature biotechnology 2023-11-16 [PMID: 37974010] (IHC, Flow Cytometry, Mouse) IHC, Flow Cytometry Mouse
Meyer, K;Lammers, NC;Bugaj, LJ;Garcia, HG;Weiner, OD; Optogenetic control of YAP reveals a dynamic communication code for stem cell fate and proliferation Nature communications 2023-10-30 [PMID: 37903793] (ICC, Mouse) ICC Mouse
Balducci, V;Scardigli, F;Harakalova, M;Peter van Tintelen, J;Doevendans, PA;Costa, KD;Turnbull, IC;P G Sluijter, J;Stillitano, F; Generation and characterization of novel human induced pluripotent stem cell (iPSC) lines originating from five asymptomatic individuals carrying the PLN-R14del pathogenic variant and a non-carrier relative Stem cell research 2023-09-21 [PMID: 37748331] (ICC, Human) ICC Human
Maura K. Schwartz, Shibi Likhite, Tatyana A. Vetter, Megan C. Baird, Vicki McGovern, Andrea Sierra Delgado et al. In-depth comparison of Anc80L65 and AAV9 retinal targeting and characterization of cross-reactivity to multiple AAV serotypes in humans Molecular Therapy - Methods & Clinical Development 2023-09-14 [PMID: 37746244]
Liu, W;Zeng, W;Kong, X;Htet, M;Yu, R;Wheeler, M;Day, JW;Wu, JC; Generation of two induced pluripotent stem cell lines from Duchenne muscular dystrophy patients Stem cell research 2023-09-18 [PMID: 37740996] (ICC, Flow Cytometry, Human) ICC, Flow Cytometry Human
Kenyu Iwatsuki, Mami Oikawa, Hisato Kobayashi, Christopher A. Penfold, Makoto Sanbo, Takuya Yamamoto et al. Rat post-implantation epiblast-derived pluripotent stem cells produce functional germ cells Cell Reports Methods 2023-08-28 [PMID: 37671016]
Jourdon, A;Wu, F;Mariani, J;Capauto, D;Norton, S;Tomasini, L;Amiri, A;Suvakov, M;Schreiner, JD;Jang, Y;Panda, A;Nguyen, CK;Cummings, EM;Han, G;Powell, K;Szekely, A;McPartland, JC;Pelphrey, K;Chawarska, K;Ventola, P;Abyzov, A;Vaccarino, FM; Modeling idiopathic autism in forebrain organoids reveals an imbalance of excitatory cortical neuron subtypes during early neurogenesis Nature neuroscience 2023-08-10 [PMID: 37563294] (IHC, Human) IHC Human
Cunningham, D;Stanberry, I;Ye, S;Alonzo, M;Zhao, MT;Garg, V;Lilly, B; Generation of iPSC line NCHi012-A from a patient with Alagille syndrome and heterozygous pathogenic variant in the JAG1 gene Stem cell research 2023-08-01 [PMID: 37549562] (ICC, Human) ICC Human
Mehmet Girgin, Nicolas Broguiere, Lorenzo Mattolini, Matthias Lutolf A New Approach to Generate Gastruloids to Develop Anterior Neural Tissues BIO-PROTOCOL 2023-07-20 [PMID: 37497450]
Show All 147 Publications.

Reviews for Otx2 Antibody (AF1979) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, pleurodeles waltl.

Reviews using AF1979:
Filter by Applications
ICC
(2)
All Applications
Filter by Species
Mouse
(1)
pleurodeles waltl
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
ICC pleurodeles waltl 03/30/2020
View

Summary

ApplicationImmunocytochemistry
Sample Testedbrain
Speciespleurodeles waltl
LotKNO0919021
Immunocytochemistry Otx2 AF1979
Enlarge
5
reviewed by:
Verified Customer
ICC Mouse 08/04/2016
View

Summary

ApplicationImmunocytochemistry
Sample TestedR1 wild type Mouse embryonic Stem Cells
SpeciesMouse
LotKNO0615111

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mFluor Violet Conjugated Antibodies

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Recent Reviews

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Verified Customer
03/30/2020
Application: ICC
Species: pleurodeles waltl

Verified Customer
08/04/2016
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol OTX2
Uniprot