Otx2 Antibody [Unconjugated]

Images

 
Otx2 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 10 µg/mL for 3 hours at room ...read more
Otx2 was detected in immersion fixed paraffin-embedded sections of mouse embryo (14 d.p.c.) using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 1 µg/mL for 1 hour at ...read more
Simple Western lane view shows lysates of IMR‑32 human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Otx2 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat ...read more
Western blot shows lysates of IMR‑32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Otx2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Otx2
Met1-Leu289
Accession # P32243
Specificity
Detects human Otx2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
OTX2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF1979 in the following applications:

Publications
Read Publications using
AF1979 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Otx2 Antibody [Unconjugated]

  • homeobox protein OTX2
  • MCOPS5
  • MGC45000
  • orthodenticle homeobox 2
  • orthodenticle homolog 2 (Drosophila)
  • Orthodenticle homolog 2
  • Otx2

Background

Otx2 is a member of the bicoid subfamily of homeodomain-containing transcription factors. It may play a role in brain and sensory organ development (1). Isoform a differs from isoform b by having an eight amino acid residue insertion between P32 and A33 of Otx2b.
  1. Nagao, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3737.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF1979
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Publications for Otx2 Antibody (AF1979)(50)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chicken, Medaka, Pan troglodytes (Chimpanzee).

We have publications tested in 9 applications: Bioassay, Differentiation, Flow Cytometry, ICC, IF, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
Bioassay
(1)
Differentiation
(1)
Flow Cytometry
(1)
ICC
(26)
IF
(1)
IHC
(13)
IHC-Fr
(3)
IHC-P
(2)
Western Blot
(2)
All Applications
Filter By Species
Human
(29)
Mouse
(17)
Rat
(1)
Chicken
(1)
Medaka
(1)
Pan troglodytes (Chimpanzee)
(1)
All Species
Showing Publications 1 - 10 of 50. Show All 50 Publications.
Publications using AF1979 Applications Species
S Lim, YJ Kim, S Park, JH Choi, Y Sung, K Nishimori, Z Kozmik, HW Lee, JW Kim mTORC1-induced retinal progenitor cell overproliferation leads to accelerated mitotic aging and degeneration of descendent M�ller glia Elife, 2021;10(0):. 2021 [PMID: 34677125] (IHC, Mouse) IHC Mouse
L Zilova, V Weinhardt, T Tavhelidse, C Schlagheck, T Thumberger, J Wittbrodt Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development Elife, 2021;10(0):. 2021 [PMID: 34252023] (IHC, Medaka) IHC Medaka
G Iannello, A Patel, D Sirabella, B Corneo, V Thaker Generation of the iPSC line CUIMCi003-A derived from a patient with severe early onset obesity Stem Cell Research, 2021;54(0):102432. 2021 [PMID: 34214899] (ICC, Human) ICC Human
A Yanagida, D Spindlow, J Nichols, A Dattani, A Smith, G Guo Naive stem cell blastocyst model captures human embryo lineage segregation Cell Stem Cell, 2021;28(6):1016-1022.e4. 2021 [PMID: 33957081] (ICC, Human) ICC Human
L Liu, SP Shenoy, JWS Jahng, Y Liu, JW Knowles, Y Zhuge, JC Wu Generation of two heterozygous MYBPC3 mutation-carrying human iPSC lines, SCVIi001-A and SCVIi002-A, for modeling hypertrophic cardiomyopathy Stem Cell Research, 2021;53(0):102279. 2021 [PMID: 33743363] (Flow Cytometry, Human) Flow Cytometry Human
MA Molè, A Weberling, R Fässler, A Campbell, S Fishel, M Zernicka-G Integrin &beta1 coordinates survival and morphogenesis of the embryonic lineage upon implantation and pluripotency transition Cell Reports, 2021;34(10):108834. 2021 [PMID: 33691117] (IHC, Mouse) IHC Mouse
H De Belly, A Stubb, A Yanagida, C Labouesse, PH Jones, EK Paluch, KJ Chalut Membrane Tension Gates ERK-Mediated Regulation of Pluripotent Cell Fate Cell Stem Cell, 2020;0(0):. 2020 [PMID: 33217323] (IHC, Mouse) IHC Mouse
T Malankhano, L Suldina, E Grigor'eva, S Medvedev, J Minina, K Morozova, E Kiseleva, S Zakian, A Malakhova A Human Induced Pluripotent Stem Cell-Derived Isogenic Model of Huntington's Disease Based on Neuronal Cells Has Several Relevant Phenotypic Abnormalities J Pers Med, 2020;10(4):. 2020 [PMID: 33182269] (ICC, Human) ICC Human
F Jacob, SR Pather, WK Huang, F Zhang, SZH Wong, H Zhou, B Cubitt, W Fan, CZ Chen, M Xu, M Pradhan, DY Zhang, W Zheng, AG Bang, H Song, J Carlos de, GL Ming Human Pluripotent Stem Cell-Derived Neural Cells and Brain Organoids Reveal SARS-CoV-2 Neurotropism Predominates in Choroid Plexus Epithelium Cell Stem Cell, 2020;0(0):. 2020 [PMID: 33010822] (IHC, Human) IHC Human
PA Barbuti, BFR Santos, CM Dording, G Cruciani, F Massart, A Hummel, R Krüger Generation of two iPS cell lines (HIHDNDi001-A and HIHDNDi001-B) from a Parkinson�s disease patient carrying the heterozygous p.A30P mutation in SNCA Stem Cell Res, 2020;48(0):101951. 2020 [PMID: 32798915] (ICC, Human) ICC Human
Show All 50 Publications.

Reviews for Otx2 Antibody (AF1979) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, pleurodeles waltl.

Reviews using AF1979:
Filter by Applications
ICC
(2)
All Applications
Filter by Species
Mouse
(1)
pleurodeles waltl
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Otx2 AF1979
Enlarge
4
reviewed by:
Verified Customer
ICC pleurodeles waltl 03/30/2020
View

Summary

ApplicationImmunocytochemistry
Sample Testedbrain
Speciespleurodeles waltl
LotKNO0919021
Immunocytochemistry Otx2 AF1979
Enlarge
5
reviewed by:
Verified Customer
ICC Mouse 08/04/2016
View

Summary

ApplicationImmunocytochemistry
Sample TestedR1 wild type Mouse embryonic Stem Cells
SpeciesMouse
LotKNO0615111

Blocking

Blocking DetailsBlocking: 0.1% Triton X in PBS with 5% donkey serum, 1 hr at RT

Primary Anitbody

Dilution Ratio1:300, 14 hrs, 4 C, Buffer: 0.1% Triton X + 5% donkey serum in PBS

Details

Detection NotesFluorescence Imaging, AF488 with EGFP filter cube, Nikon Eclipse Ti microscope, Exposure time: 1 sec
Fixation DetailsFixed for 10 mins with 4% formaldehyde at room temperature. Permeablization with 0.2% TritonX in PBS for 15 mins at RT
Wash DescriptionPBS,~1 min, 3 times

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Otx2 Antibody (AF1979) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF1979

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Bioinformatics Tool for Otx2 Antibody (AF1979)

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
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Verified Customer
03/30/2020
Application: ICC
Species: pleurodeles waltl

Verified Customer
08/04/2016
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol OTX2
Uniprot