NKX6.1 Antibody

Western Blot: NKX6.1 Antibody [NBP1-49672] - Analysis of Nkx6.1 in human skeletal muscle.

Immunocytochemistry/Immunofluorescence: NKX6.1 Antibody [NBP1-49672] - Nkx6.1 antibody was tested at 1:250 in INS-1 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40X.

Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-49672] - Paraffin embeded rat pancreatic tissue at 40x resolution stained for insulin (red) and NKX6.1 (NBP1-49672, green). IHC-P image submitted by a verified customer review.

Immunohistochemistry-Frozen: NKX6.1 Antibody [NBP1-49672] - Analysis of NKX6.1 in mouse pancreas frozen tissue section using anti-NKX6.1 antibody. IHC-Fr image submitted by a verified customer review.

Immunohistochemistry: NKX6.1 Antibody [NBP1-49672] - Staining of Nkx6.1 in mouse intestine using DAB with hematoxylin counterstain.

Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-49672] - Human fetal pancreas stained for Nkx6.1, green, and PDX1, grey. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-49672] - Mouse pancreas tissue. IHC-P image submitted by a verified customer review.

Simple Western: NKX6.1 Antibody [NBP1-49672] - Image shows a specific band for NKX6.1 in 0.5 mg/mL of BTC-6 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

NKX6.1 Antibody Summary

• Immunogen: A genomic peptide made to an internal region of the human Nkx6.1 protein (within residues 50-200). [Swiss-Prot P78426]
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NKX6-1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100 - 1:2000
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:50 - 1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50 - 1:100
  • In vitro assay
• Application Notes: Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use In vitro assay reported in scientific literature (PMID: 30471425).

Reactivity Notes

Rat reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for NKX6.1 Antibody

• Homeobox protein NK-6 homolog A
• NK homeo box, family 6, member A
• NK homeobox (Drosophila), family 6, A
• NK homeobox, family 6, A
• NK6 homeobox 1
• NK6 transcription factor homolog A
• NK6 transcription factor related, locus 1 (Drosophila)
• NK6 transcription factor related, locus 1
• NKX6.1
• NKX6-1
• NKX6A
• NKX6Ahomeobox protein Nkx-6.1

Background

NKX6.1 (homeobox protein Nkx-6.1) is a transcription factor that binds to specific A/T-rich sequences in promoter regions of several genes and is known for its involvement in transcriptional regulation in islet beta cells via NKX6.1-insulin promoter binding mediated control of insulin gene. Together with NKX2-2 as well as IRX3, NKX6.1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. It belongs to neuronal progenitor factors class II proteins that are induced by SHH signals and is a member of the homeodomain family of transcription factors responsible for multiple organ systems development. The pancreatic development involves many such homeodomain proteins including Hb9, Pdx1, Pax4, Nkx2.2, and importantly on Nkx6.1 and mice deficient for Nkx6.1 display a dramatic reduction in beta-cell numbers in pancreas with near-normal development of other glandular cell types. Moreover, Nkx6.1 deficient mice have significant defects involving V2 interneuron and motor neuron specification in the ventral spinal cord, thereby emphasizing an important parallel in developmental pathways leading to beta-cells and neurons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NKX6.1 Antibody (NBP1-49672)(10)

Publications using NBP1-49672

• Morello F, Borshagovski D, Survila M et al. Molecular Fingerprint and Developmental Regulation of the Tegmental GABAergic and Glutamatergic Neurons Derived from the Anterior Hindbrain Cell Rep Oct 13 2020 [PMID: 33053343]
• Deng H, Yang F, Ma X et al. Long-Term Liraglutide Administration Induces Pancreas Neogenesis in Adult T2DM Mice Cell Transplant Jun 26 2020 [PMID: 32584149] (ICC/IF, Mouse)
• Spaeth JM, Liu JH, Peters D et al. The Pdx1 Bound Swi/Snf Chromatin Remodeling Complex Regulates Pancreatic Progenitor Cell Proliferation and Mature Islet beta Cell Function Diabetes Jun 14 2019 [PMID: 31201281] (IHC-Fr, IHC, Mouse)
• Wilson, CS;Spaeth, JM;Karp, J;Stocks, BT;Hoopes, EM;Stein, RW;Moore, DJ; B lymphocytes protect islet beta cells in diabetes prone NOD mice treated with imatinib JCI Insight Apr 9 2019 [PMID: 30964447] (IHC, Mouse)
• Griffiths WJ, Crick PJ, Meljon A et al. Additional pathways of sterol metabolism: Evidence from analysis of Cyp27a1-/- mouse brain and plasma. Molecular Therapy - Nucleic Acids. Nov 1 2018 [PMID: 30471425] (In vitro, Mouse)
• Mohan V, Radha V, Nguyen TT, Stawiski EW. Comprehensive genomic analysis identifies pathogenic variants in maturity-onset diabetes of the young (MODY) patients in South India. BMC Med. Genet. 2018 Feb 13 [PMID: 29439679] (WB, Human)
• Kropp PA, Dunn JC, Carboneau BA et al. Cooperative function of Pdx1 and Oc1 in multipotent pancreatic progenitors impacts postnatal islet maturation and adaptability. American Journal of Physiology Endocrinology and Metabolism 2017 Dec 12 [PMID: 29351489] (Mouse)
• Jason Spaeth, Manisha Gupte, Mark Perelis et al. Defining a Novel Role for the PDX1 Transcription Factor in Islet Cell Maturation and Proliferation During Weaning. American Diabetes Association 2017 Jul 03 [PMID: 28705881] (ICC/IF, Mouse)
• Theofilopoulos S, Griffiths WJ, Crick PJ et al. Cholestenoic acids regulate motor neuron survival via liver X receptors. J. Clin. Invest. 2014 Nov 03 [PMID: 25271621] (ICC/IF, Mouse)
  
  Details:
  NKX6.1 antibody used for ICC-IF on primary midbrain cultures (mouse E11.5) treated or not with 3beta-7alpha-diHCA, 3 betaH,7O-CA, 3 beta,7 beta-diHCA or 3beta-HCA - 4% PFA fixation, 1 hour bocking with 5% normal goat serum/PBS, primary incubated for ON in PBSpH 7.4 containing 0.3% Triton X-100 and 1% BSA (Figure 4 B).
• Theofilopoulos S, Wang Y, Kitambi SS et al. Brain endogenous liver X receptor ligands selectively promote midbrain neurogenesis Nat Chem Biol 2012 Dec 23 [PMID: 23292650] (ICC/IF, Mouse)

Reviews for NKX6.1 Antibody (NBP1-49672) (4)

Average Rating: 4.8

(Based on 4 reviews)

We have 4 reviews tested in 3 species: Human, Mouse, Rat.

reviewed by: Jonatan Dereke IHC-P Rat 04/29/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Pancreas tissue
• Species: Rat
• Lot: D-4

Comments

• Comments: We used a 1:1600 dilution for our assays.

reviewed by: Anonymous IHC-P Mouse 01/08/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse Pancreas
• Species: Mouse
• Lot: C-1

reviewed by: Anonymous IF Mouse 10/30/2015

Summary

• Application: Immunofluorescence
• Species: Mouse
• File: View PDF

reviewed by: Anonymous IHC-P Human 09/24/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human fetal pancreas
• Species: Human
• Lot: B-1

Blocking

• Blocking Details: BSA 2%

Primary Anitbody

• Dilution Ratio: 1/100 dilution. Incubation for 1 hour RT.

Secondary Antibody

• Secondary Description: Alexa fluor 488 anti-rabbit
• Secondary Concentration: 1/500

Details

• Fixation Details: The sample was fixed in neutral formalin and paraffin-embedded. Perform antigen retrieval low pH (citrate buffer).

FAQs for NKX6.1 Antibody (NBP1-49672). (Showing 1 - 1 of 1 FAQs).

1. Has this antibody been tested on cryo sections for immunohistochemistry?
   • Unfortunately the antibody has never been tested with any frozen section (so called IHC-Fr). This antibody had been tested, in lab, for using in WB, ICC/IF, and IHC-P as these were already posted on the web. Our company use "IHC" just for a faster indexing/searching or browsing the webpages. In theory, when an antibody product can be used in IHC-P, usually it can be used in IHC-Fr with the same dilutions; but not the way around. Therefore you could consider to include NBP1-49672 for using in your ongoing IHC-Fr experiments.

Bioinformatics

• Gene Symbol: NKX6-1
• Entrez:
  • 4825(Human)
  • 18096(Mouse)
• Uniprot:
  • P78426()