Western Blot: NKX6.1 Antibody [NBP1-49672] - WB analysis of Nkx6.1 in human skeletal muscle.
Immunocytochemistry/ Immunofluorescence: NKX6.1 Antibody [NBP1-49672] - Nkx6.1 antibody was tested at 1:250 in INS-1 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-49672] - Human fetal pancreas stained for Nkx6.1, green, and PDX1, grey. Image from verified customer review.
Immunohistochemistry-Frozen: NKX6.1 Antibody [NBP1-49672] - Analysis of NKX6.1 in mouse pancreas frozen tissue section using anti-NKX6.1 antibody. Image from verified customer review.
Immunohistochemistry: NKX6.1 Antibody [NBP1-49672] - IHC staining of Nkx6.1 in mouse intestine using DAB with hematoxylin counterstain.
Simple Western: NKX6.1 Antibody [NBP1-49672] - Simple Western lane view shows a specific band for NKX6.1 in 0.5 mg/ml of BTC-6 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Immunogen affinity purified
Western Blot 1:100-1:2000
Simple Western 1:100
This Nkx6.1 antibody is useful for Immunohistochemistry-paraffin embedded sections. In IHC-P, staining was observed in the nucleus of mouse intestines. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Read 2 Reviews rated 5 using NBP1-49672 in the following applications:
Manufactured by Genomic Antibody Technology™. GAT FAQs
Alternate Names for NKX6.1 Antibody
Homeobox protein NK-6 homolog A
NK homeo box, family 6, member A
NK homeobox (Drosophila), family 6, A
NK homeobox, family 6, A
NK6 homeobox 1
NK6 transcription factor homolog A
NK6 transcription factor related, locus 1 (Drosophila)
NK6 transcription factor related, locus 1
NKX6Ahomeobox protein Nkx-6.1
NKX6.1 (homeobox protein Nkx-6.1) is a transcription factor that binds to specific A/T-rich sequences in promoter regions of several genes and is known for its involvement in transcriptional regulation in islet beta cells via NKX6.1-insulin promoter binding mediated control of insulin gene. Together with NKX2-2 as well as IRX3, NKX6.1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. It belongs to neuronal progenitor factors class II proteins that are induced by SHH signals and is a member of the homeodomain family of transcription factors responsible for multiple organ systems development. The pancreatic development involves many such homeodomain proteins including Hb9, Pdx1, Pax4, Nkx2.2, and importantly on Nkx6.1 and mice deficient for Nkx6.1 display a dramatic reduction in beta-cell numbers in pancreas with near-normal development of other glandular cell types. Moreover, Nkx6.1 deficient mice have significant defects involving V2 interneuron and motor neuron specification in the ventral spinal cord, thereby emphasizing an important parallel in developmental pathways leading to beta-cells and neurons.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for NKX6.1 Antibody (NBP1-49672). (Showing 1 - 1 of 1 FAQs).
Has this antibody been tested on cryo sections for immunohistochemistry?
Unfortunately the antibody has never been tested with any frozen section (so called IHC-Fr). This antibody had been tested, in lab, for using in WB, ICC/IF, and IHC-P as these were already posted on the web. Our company use "IHC" just for a faster indexing/searching or browsing the webpages. In theory, when an antibody product can be used in IHC-P, usually it can be used in IHC-Fr with the same dilutions; but not the way around. Therefore you could consider to include NBP1-49672 for using in your ongoing IHC-Fr experiments.