NKX6.1 Antibody

Images

 
Western Blot: NKX6.1 Antibody [NBP1-49672] - WB analysis of Nkx6.1 in human skeletal muscle.
Immunocytochemistry/ Immunofluorescence: NKX6.1 Antibody [NBP1-49672] - Nkx6.1 antibody was tested at 1:250 in INS-1 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-49672] - Human fetal pancreas stained for Nkx6.1, green, and PDX1, grey. Image from verified customer review.
Immunohistochemistry-Frozen: NKX6.1 Antibody [NBP1-49672] - Analysis of NKX6.1 in mouse pancreas frozen tissue section using anti-NKX6.1 antibody. Image from verified customer review.
Immunohistochemistry: NKX6.1 Antibody [NBP1-49672] - IHC staining of Nkx6.1 in mouse intestine using DAB with hematoxylin counterstain.
Simple Western: NKX6.1 Antibody [NBP1-49672] - Simple Western lane view shows a specific band for NKX6.1 in 0.5 mg/ml of BTC-6 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

NKX6.1 Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human Nkx6.1 protein (within residues 50-200). [Swiss-Prot P78426]
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Gene
NKX6-1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:100
Application Notes
This Nkx6.1 antibody is useful for Immunohistochemistry-paraffin embedded sections. In IHC-P, staining was observed in the nucleus of mouse intestines. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-49672 in the following applications:

Publications
Read Publications using
NBP1-49672 in the following applications:

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for NKX6.1 Antibody

  • Homeobox protein NK-6 homolog A
  • NK homeo box, family 6, member A
  • NK homeobox (Drosophila), family 6, A
  • NK homeobox, family 6, A
  • NK6 homeobox 1
  • NK6 transcription factor homolog A
  • NK6 transcription factor related, locus 1 (Drosophila)
  • NK6 transcription factor related, locus 1
  • NKX6.1
  • NKX6-1
  • NKX6A
  • NKX6Ahomeobox protein Nkx-6.1

Background

NKX6.1 (homeobox protein Nkx-6.1) is a transcription factor that binds to specific A/T-rich sequences in promoter regions of several genes and is known for its involvement in transcriptional regulation in islet beta cells via NKX6.1-insulin promoter binding mediated control of insulin gene. Together with NKX2-2 as well as IRX3, NKX6.1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. It belongs to neuronal progenitor factors class II proteins that are induced by SHH signals and is a member of the homeodomain family of transcription factors responsible for multiple organ systems development. The pancreatic development involves many such homeodomain proteins including Hb9, Pdx1, Pax4, Nkx2.2, and importantly on Nkx6.1 and mice deficient for Nkx6.1 display a dramatic reduction in beta-cell numbers in pancreas with near-normal development of other glandular cell types. Moreover, Nkx6.1 deficient mice have significant defects involving V2 interneuron and motor neuron specification in the ventral spinal cord, thereby emphasizing an important parallel in developmental pathways leading to beta-cells and neurons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NKX6.1 Antibody (NBP1-49672)(2)

Reviews for NKX6.1 Antibody (NBP1-49672) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 reviews tested in 2 application: IF, IHC-P.

Reviews using NBP1-49672:
Filter by Applications
IF
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence NKX6.1 NBP1-49672
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reviewed by:
Anonymous
IF Mouse 10/30/2015
View

Summary

ApplicationImmunofluorescence
SpeciesMouse
FileView PDF
Immunohistochemistry-Paraffin NKX6.1 NBP1-49672
Enlarge
reviewed by:
Anonymous
IHC-P Human 09/24/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman fetal pancreas
SpeciesHuman
LotB-1

Blocking

Blocking DetailsBSA 2%

Primary Anitbody

Dilution Ratio1/100 dilution. Incubation for 1 hour RT.

Secondary Antibody

Secondary DescriptionAlexa fluor 488 anti-rabbit
Secondary Concentration1/500

Details

Fixation DetailsThe sample was fixed in neutral formalin and paraffin-embedded. Perform antigen retrieval low pH (citrate buffer).

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NKX6.1 Antibody (NBP1-49672). (Showing 1 - 1 of 1 FAQs).

  1. Has this antibody been tested on cryo sections for immunohistochemistry?
    • Unfortunately the antibody has never been tested with any frozen section (so called IHC-Fr). This antibody had been tested, in lab, for using in WB, ICC/IF, and IHC-P as these were already posted on the web. Our company use "IHC" just for a faster indexing/searching or browsing the webpages. In theory, when an antibody product can be used in IHC-P, usually it can be used in IHC-Fr with the same dilutions; but not the way around. Therefore you could consider to include NBP1-49672 for using in your ongoing IHC-Fr experiments.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-49672AF405
Alexa Fluor 488 Labeled NBP1-49672AF488
Alexa Fluor 647 Labeled NBP1-49672AF647
Alexa Fluor 700 Labeled NBP1-49672AF700
Biotin Labeled NBP1-49672B
DyLight 405 Labeled NBP1-49672V
DyLight 488 Labeled NBP1-49672G
DyLight 550 Labeled NBP1-49672R
DyLight 650 Labeled NBP1-49672C
FITC Labeled NBP1-49672F
HRP Labeled NBP1-49672H

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Contact Information

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
10/30/2015
Application: IF
Species: Mouse

Anonymous
09/24/2014
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol NKX6-1
Entrez
Uniprot

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