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NKX6.1 Antibody

 (8 Publications)
 (4 Reviews)
 
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Western Blot: NKX6.1 Antibody [NBP1-49672] - Analysis of Nkx6.1 in human skeletal muscle.
Western Blot: NKX6.1 Antibody [NBP1-49672] - Analysis of Nkx6.1 in human skeletal muscle.
Immunocytochemistry/ Immunofluorescence: NKX6.1 Antibody [NBP1-49672] - Nkx6.1 antibody was tested at 1:250 in INS-1 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-49672] - Paraffin embeded rat pancreatic tissue at 40x resolution stained for insulin (red) and NKX6.1 (NBP1-49672, green). IHC-P image submitted by a verified ...read more
Immunohistochemistry-Frozen: NKX6.1 Antibody [NBP1-49672] - Analysis of NKX6.1 in mouse pancreas frozen tissue section using anti-NKX6.1 antibody. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry: NKX6.1 Antibody [NBP1-49672] - Staining of Nkx6.1 in mouse intestine using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-49672] - Human fetal pancreas stained for Nkx6.1, green, and PDX1, grey. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-49672] - Mouse pancreas tissue. IHC-P image submitted by a verified customer review.
Simple Western: NKX6.1 Antibody [NBP1-49672] - Image shows a specific band for NKX6.1 in 0.5 mg/mL of BTC-6 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-49672AF350
Alexa Fluor 405
NBP1-49672AF405
Alexa Fluor 488
NBP1-49672AF488
Alexa Fluor 532
NBP1-49672AF532
Alexa Fluor 594
NBP1-49672AF594
Alexa Fluor 647
NBP1-49672AF647
Alexa Fluor 700
NBP1-49672AF700
Alexa Fluor 750
NBP1-49672AF750
Biotin
NBP1-49672B
DyLight 350
NBP1-49672UV
DyLight 405
NBP1-49672V
DyLight 488
NBP1-49672G
DyLight 550
NBP1-49672R
DyLight 594
NBP1-49672DL594
DyLight 650
NBP1-49672C
DyLight 680
NBP1-49672FR
DyLight 755
NBP1-49672IR
FITC
NBP1-49672F
HRP
NBP1-49672H
Janelia Fluor 549
NBP1-49672JF549
Janelia Fluor 646
NBP1-49672JF646
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NKX6.1 Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human Nkx6.1 protein (within residues 50-200). [Swiss-Prot P78426]
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NKX6-1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:50 - 1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50 - 1:100
  • In vitro assay
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use In vitro assay reported in scientific literature (PMID: 30471425).
Reviewed Applications
Read 4 Reviews rated 4.8
using
NBP1-49672 in the following applications:
Publications
Read Publications using
NBP1-49672 in the following applications:

Reactivity Notes

Rat reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for NKX6.1 Antibody

  • Homeobox protein NK-6 homolog A
  • NK homeo box, family 6, member A
  • NK homeobox (Drosophila), family 6, A
  • NK homeobox, family 6, A
  • NK6 homeobox 1
  • NK6 transcription factor homolog A
  • NK6 transcription factor related, locus 1 (Drosophila)
  • NK6 transcription factor related, locus 1
  • NKX6.1
  • NKX6-1
  • NKX6A
  • NKX6Ahomeobox protein Nkx-6.1

Background

NKX6.1 (homeobox protein Nkx-6.1) is a transcription factor that binds to specific A/T-rich sequences in promoter regions of several genes and is known for its involvement in transcriptional regulation in islet beta cells via NKX6.1-insulin promoter binding mediated control of insulin gene. Together with NKX2-2 as well as IRX3, NKX6.1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. It belongs to neuronal progenitor factors class II proteins that are induced by SHH signals and is a member of the homeodomain family of transcription factors responsible for multiple organ systems development. The pancreatic development involves many such homeodomain proteins including Hb9, Pdx1, Pax4, Nkx2.2, and importantly on Nkx6.1 and mice deficient for Nkx6.1 display a dramatic reduction in beta-cell numbers in pancreas with near-normal development of other glandular cell types. Moreover, Nkx6.1 deficient mice have significant defects involving V2 interneuron and motor neuron specification in the ventral spinal cord, thereby emphasizing an important parallel in developmental pathways leading to beta-cells and neurons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western blot shows lysates of beta TC‑6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PDX‑1/IPF1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
PDX‑1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Simple Western lane view shows lysates of beta TC‑6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PDX‑1/IPF1 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
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(E2-E3 (same as INS04 ...
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Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Insulin E2E3, human pancreas (FFPE), HIER pH 9 antigen retrieval. Image from verified customer review.
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)
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Western blot shows lysates of human placenta and prostate tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Neurogenin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3444) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neurogenin‑3 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Western Blot: PDHX Antibody [NBP1-84079] - Analysis using Anti-PDHX antibody NBP1-84079 (A) shows similar pattern to independent antibody NBP1-84080 (B).
Immunohistochemistry-Paraffin: PDHX Antibody [NBP1-84079] - Staining of human kidney.
Immunohistochemistry-Paraffin: PDHX Antibody [NBP1-84079] - Staining of human stomach shows strong cytoplasmic(granular pattern) and nuclear positivity in glandular cells.
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NBP1-82554
Western Blot: NKX2.2 Antibody [NBP1-82554] - Analysis in human cell line U-138 MG.
Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554] - Staining of human cerebellum shows moderate postivity in cells in molecular layer.
Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554] - Staining of human pancreas shows strong nuclear positivity in islet cells.
(2 Reviews)
(3 Publications)
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NBP2-19711
Western Blot: Pax6 Antibody [NBP2-19711] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NBP2-19711] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PAX6 protein stained by PAX6 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 um.
Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Mouse retina. Green: PAX6 protein stained by PAX6 antibody diluted at 1:250. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Sonic Hedgehog/Shh
[Unconjugated]
1845-SH
1 μg/lane of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.
MALDI-TOF analysis of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus. The major peak corresponds to the calculated molecular mass, 19814 Da. The minor peak at 20029  is a matrix-associated artifact of the MALDI-TOF.
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Immunocytochemistry/Immunofluorescence: Glut2 Antibody [NBP2-22218] - Glut2 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
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(3H8)
H00004760-M01
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(5F1)
NBP2-75507
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Immunocytochemistry/Immunofluorescence: Glucagon Antibody (5F1) [NBP2-75507] - Staining Glucagon (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Glucagon Antibody (5F1) [NBP2-75507] - Analysis of paraffin-embedded rat pancreas tissue using anti-Glucagon antibody. Counter stained with hematoxylin.
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AF1837
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Recombinant Human
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H00084504-Q01
12.5% SDS-PAGE Stained with Coomassie Blue.
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Pax4 Antibody
AF2614
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Glucokinase/GCK
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NBP1-33144
Western Blot: Glucokinase/GCK Antibody [NBP1-33144] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glucokinase antibody [N1C1-2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Glucokinase/GCK Antibody [NBP1-33144] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glucokinase protein stained by Glucokinase antibody [N1C1-2] diluted at 1:500.Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Glucokinase/GCK Antibody [NBP1-33144] - MCF7 xenograft, using Glucokinase antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Applications: WB, ICC/IF, IHC, IHC-P
KLRG1 Antibody
(1151A) [Unconjugated
MAB6944
Mouse splenocytes were stained with Rat Anti-Mouse CD161/NK1.1 APC‑conjugated Monoclonal Antibody (Catalog # FAB7614A) and either (A) Rabbit Anti-Mouse KLRG1 Monoclonal Antibody (Catalog # MAB6944) or (B) Normal Rabbit IgG Control (Catalog #AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).
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Somatostatin
Antibody (7G5)
NBP2-37447
Western Blot: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis using SST mAb against human SST recombinant protein. (Expected MW is 38.2 kDa)
Immunohistochemistry-Paraffin: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis of lung cancer tissues using SST mouse mAb with DAB staining.
Flow Cytometry: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis of HepG2 cells using SST mouse mAb (green) and negative control (red).
(1 Review)
(1 Publication)
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HNF-3 beta/FoxA2
Antibody [Unconjugate ...
AF2400
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HNF‑3 beta /FoxA2 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Mouse splenocytes were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HNF‑3 beta /FoxA2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The E-RABP promoter was detected by standard PCR.
HNF‑3 beta /FoxA2 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(1 Review)
(36 Publications)
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Applications: WB, IHC, ChIP, ICC
Olig2 Antibody
[Unconjugated]
AF2418
A172 human glioblastoma cell line were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Olig2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The p21 promoter was detected by standard PCR.
Olig2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Olig2 and Oligodendrocyte Marker O4 were detected in 7 day differentiated rat cortical stem cells using 10 µg/mL Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) and 10 µg/mL Goat Anti-Human/Mouse/Rat/Chicken O4 Monoclonal Antibody (Catalog # MAB1326). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Olig2 using the NorthernLights™ 637-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL002), and stained for O4 using an anti-mouse IgM secondary antibody (pseudo-stained green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(6 Reviews)
(42 Publications)
Species: Hu, Mu, Rt
Applications: WB, IHC, ChIP, ICC
Amylin Antibody
NBP2-33680
Immunohistochemistry-Paraffin: Amylin Antibody [NBP2-33680] - Staining of human placenta shows no positivity in trophoblastic cells as expected.
Immunohistochemistry: Amylin Antibody [NBP2-33680] - Staining of human pancreas shows distinct cytoplasmic positivity in islets of Langerhans.
Immunohistochemistry-Paraffin: Amylin Antibody [NBP2-33680] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.
Species: Hu
Applications: IHC, IHC-P
Pancreatic
Polypeptide/PP Antibo
NB100-1793
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Staining of paraffin embedded Human Intestine with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Frozen: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Analysis of Pancreatic Polypeptide in mouse adult pancreas tissue. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Adult macaque pancrease paraffin sections following standard protocols. IHC-P image submitted by a verified customer review.
(3 Reviews)
(14 Publications)
Species: Hu, Mu, Pm
Applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA

Publications for NKX6.1 Antibody (NBP1-49672)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, In vitro, WB.


Filter By Application
ICC/IF
(3)
IHC
(2)
IHC-Fr
(1)
In vitro
(1)
WB
(1)
All Applications
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Human
(1)
Mouse
(7)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP1-49672 Applications Species
Spaeth JM, Liu JH, Peters D et al. The Pdx1 Bound Swi/Snf Chromatin Remodeling Complex Regulates Pancreatic Progenitor Cell Proliferation and Mature Islet beta Cell Function Diabetes Jun 14 2019 [PMID: 31201281] (IHC-Fr, IHC, Mouse) IHC-Fr, IHC Mouse
Wilson, CS;Spaeth, JM;Karp, J;Stocks, BT;Hoopes, EM;Stein, RW;Moore, DJ; B lymphocytes protect islet beta cells in diabetes prone NOD mice treated with imatinib JCI Insight Apr 9 2019 [PMID: 30964447] (IHC, Mouse) IHC Mouse
Griffiths WJ, Crick PJ, Meljon A et al. Additional pathways of sterol metabolism: Evidence from analysis of Cyp27a1-/- mouse brain and plasma. Molecular Therapy - Nucleic Acids. Nov 1 2018 [PMID: 30471425] (In vitro, Mouse) In vitro Mouse
Mohan V, Radha V, Nguyen TT, Stawiski EW. Comprehensive genomic analysis identifies pathogenic variants in maturity-onset diabetes of the young (MODY) patients in South India. BMC Med. Genet. 2018 Feb 13 [PMID: 29439679] (WB, Human) WB Human
Kropp PA, Dunn JC, Carboneau BA et al. Cooperative function of Pdx1 and Oc1 in multipotent pancreatic progenitors impacts postnatal islet maturation and adaptability. American Journal of Physiology Endocrinology and Metabolism 2017 Dec 12 [PMID: 29351489] (Mouse) Mouse
Jason Spaeth, Manisha Gupte, Mark Perelis et al. Defining a Novel Role for the PDX1 Transcription Factor in Islet Cell Maturation and Proliferation During Weaning. American Diabetes Association 2017 Jul 03 [PMID: 28705881] (ICC/IF, Mouse) ICC/IF Mouse
Theofilopoulos S, Griffiths WJ, Crick PJ et al. Cholestenoic acids regulate motor neuron survival via liver X receptors. J. Clin. Invest. 2014 Nov 03 [PMID: 25271621] (ICC/IF, Mouse)

Details:
NKX6.1 antibody used for ICC-IF on primary midbrain cultures (mouse E11.5) treated or not with 3beta-7alpha-diHCA, 3 betaH,7O-CA, 3 beta,7 beta-diHCA or 3beta-HCA - 4% PFA fixation, 1 hour bocking with 5% normal goat serum/PBS, primary incubated for ON in PBSpH 7.4 containing 0.3% Triton X-100 and 1% BSA (Figure 4 B). 		ICC/IF Mouse
Theofilopoulos S, Wang Y, Kitambi SS et al. Brain endogenous liver X receptor ligands selectively promote midbrain neurogenesis Nat Chem Biol 2012 Dec 23 [PMID: 23292650] (ICC/IF, Mouse) ICC/IF Mouse

Reviews for NKX6.1 Antibody (NBP1-49672) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NBP1-49672:
Filter by Applications
IHC-P
(3)
IF
(1)
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Human
(1)
Mouse
(2)
Rat
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin NKX6.1 NBP1-49672
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reviewed by:
Jonatan Dereke
 IHC-P Rat 04/29/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedPancreas tissue
SpeciesRat
LotD-4

Comments

CommentsWe used a 1:1600 dilution for our assays.
Immunohistochemistry-Paraffin NKX6.1 NBP1-49672
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reviewed by:
Anonymous
 IHC-P Mouse 01/08/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Pancreas
SpeciesMouse
LotC-1
Immunofluorescence NKX6.1 NBP1-49672
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reviewed by:
Anonymous
 IF Mouse 10/30/2015
View

Summary

ApplicationImmunofluorescence
SpeciesMouse
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Immunohistochemistry-Paraffin NKX6.1 NBP1-49672
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reviewed by:
Anonymous
 IHC-P Human 09/24/2014
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman fetal pancreas
SpeciesHuman
LotB-1

Blocking

Blocking DetailsBSA 2%

Primary Anitbody

Dilution Ratio1/100 dilution. Incubation for 1 hour RT.

Secondary Antibody

Secondary DescriptionAlexa fluor 488 anti-rabbit
Secondary Concentration1/500

Details

Fixation DetailsThe sample was fixed in neutral formalin and paraffin-embedded. Perform antigen retrieval low pH (citrate buffer).

Product General Protocols

View specific protocols for NKX6.1 Antibody (NBP1-49672): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NKX6.1 Antibody (NBP1-49672). (Showing 1 - 1 of 1 FAQs).

  1. Has this antibody been tested on cryo sections for immunohistochemistry?
    • Unfortunately the antibody has never been tested with any frozen section (so called IHC-Fr). This antibody had been tested, in lab, for using in WB, ICC/IF, and IHC-P as these were already posted on the web. Our company use "IHC" just for a faster indexing/searching or browsing the webpages. In theory, when an antibody product can be used in IHC-P, usually it can be used in IHC-Fr with the same dilutions; but not the way around. Therefore you could consider to include NBP1-49672 for using in your ongoing IHC-Fr experiments.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-49672AF350
Alexa Fluor 405 NBP1-49672AF405
Alexa Fluor 488 NBP1-49672AF488
Alexa Fluor 532 NBP1-49672AF532
Alexa Fluor 594 NBP1-49672AF594
Alexa Fluor 647 NBP1-49672AF647
Alexa Fluor 700 NBP1-49672AF700
Alexa Fluor 750 NBP1-49672AF750
Biotin NBP1-49672B
DyLight 350 NBP1-49672UV
DyLight 405 NBP1-49672V
DyLight 488 NBP1-49672G
DyLight 550 NBP1-49672R
DyLight 594 NBP1-49672DL594
DyLight 650 NBP1-49672C
DyLight 680 NBP1-49672FR
DyLight 755 NBP1-49672IR
FITC NBP1-49672F
HRP NBP1-49672H
Janelia Fluor 549 NBP1-49672JF549
Janelia Fluor 646 NBP1-49672JF646
Learn about Custom Antibody Labeling

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Bioinformatics Tool for NKX6.1 Antibody (NBP1-49672)

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Diseases for NKX6.1 Antibody (NBP1-49672)

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Pathways for NKX6.1 Antibody (NBP1-49672)

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PTMs for NKX6.1 Antibody (NBP1-49672)

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Research Areas for NKX6.1 Antibody (NBP1-49672)

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Contact Information

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Product PDFs

  IHC-P Protocol
  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

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Recent Reviews

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Immunohistochemistry-Paraffin NKX6.1 NBP1-49672
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 Jonatan Dereke
04/29/2020
Application: IHC-P
Species: Rat
read this review
Immunohistochemistry-Paraffin NKX6.1 NBP1-49672
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 Anonymous
01/08/2019
Application: IHC-P
Species: Mouse
read this review
Immunofluorescence NKX6.1 NBP1-49672
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 Anonymous
10/30/2015
Application: IF
Species: Mouse
read this review

Bioinformatics

Gene Symbol NKX6-1
Entrez
Uniprot
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