NKX6.1 Antibody

Images

 
Western Blot: NKX6.1 Antibody [NBP1-49672] - Analysis of Nkx6.1 in human skeletal muscle.
Immunocytochemistry/ Immunofluorescence: NKX6.1 Antibody [NBP1-49672] - Nkx6.1 antibody was tested at 1:250 in INS-1 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-49672] - Paraffin embeded rat pancreatic tissue at 40x resolution stained for insulin (red) and NKX6.1 (NBP1-49672, green). IHC-P image submitted by a verified ...read more
Immunohistochemistry-Frozen: NKX6.1 Antibody [NBP1-49672] - Analysis of NKX6.1 in mouse pancreas frozen tissue section using anti-NKX6.1 antibody. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry: NKX6.1 Antibody [NBP1-49672] - Staining of Nkx6.1 in mouse intestine using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-49672] - Human fetal pancreas stained for Nkx6.1, green, and PDX1, grey. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-49672] - Mouse pancreas tissue. IHC-P image submitted by a verified customer review.
Simple Western: NKX6.1 Antibody [NBP1-49672] - Image shows a specific band for NKX6.1 in 0.5 mg/mL of BTC-6 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

NKX6.1 Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human Nkx6.1 protein (within residues 50-200). [Swiss-Prot P78426]
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NKX6-1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:50 - 1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50 - 1:100
  • In vitro assay
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use In vitro assay reported in scientific literature (PMID: 30471425).
Reviewed Applications
Read 4 Reviews rated 4.8
using
NBP1-49672 in the following applications:

Publications
Read Publications using
NBP1-49672 in the following applications:

Reactivity Notes

Rat reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for NKX6.1 Antibody

  • Homeobox protein NK-6 homolog A
  • NK homeo box, family 6, member A
  • NK homeobox (Drosophila), family 6, A
  • NK homeobox, family 6, A
  • NK6 homeobox 1
  • NK6 transcription factor homolog A
  • NK6 transcription factor related, locus 1 (Drosophila)
  • NK6 transcription factor related, locus 1
  • NKX6.1
  • NKX6-1
  • NKX6A
  • NKX6Ahomeobox protein Nkx-6.1

Background

NKX6.1 (homeobox protein Nkx-6.1) is a transcription factor that binds to specific A/T-rich sequences in promoter regions of several genes and is known for its involvement in transcriptional regulation in islet beta cells via NKX6.1-insulin promoter binding mediated control of insulin gene. Together with NKX2-2 as well as IRX3, NKX6.1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. It belongs to neuronal progenitor factors class II proteins that are induced by SHH signals and is a member of the homeodomain family of transcription factors responsible for multiple organ systems development. The pancreatic development involves many such homeodomain proteins including Hb9, Pdx1, Pax4, Nkx2.2, and importantly on Nkx6.1 and mice deficient for Nkx6.1 display a dramatic reduction in beta-cell numbers in pancreas with near-normal development of other glandular cell types. Moreover, Nkx6.1 deficient mice have significant defects involving V2 interneuron and motor neuron specification in the ventral spinal cord, thereby emphasizing an important parallel in developmental pathways leading to beta-cells and neurons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NKX6.1 Antibody (NBP1-49672)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, In vitro, WB.


Filter By Application
ICC/IF
(3)
IHC
(2)
IHC-Fr
(1)
In vitro
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(7)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP1-49672 Applications Species
Spaeth JM, Liu JH, Peters D et al. The Pdx1 Bound Swi/Snf Chromatin Remodeling Complex Regulates Pancreatic Progenitor Cell Proliferation and Mature Islet beta Cell Function Diabetes Jun 14 2019 [PMID: 31201281] (IHC-Fr, IHC, Mouse) IHC-Fr, IHC Mouse
Wilson, CS;Spaeth, JM;Karp, J;Stocks, BT;Hoopes, EM;Stein, RW;Moore, DJ; B lymphocytes protect islet beta cells in diabetes prone NOD mice treated with imatinib JCI Insight Apr 9 2019 [PMID: 30964447] (IHC, Mouse) IHC Mouse
Griffiths WJ, Crick PJ, Meljon A et al. Additional pathways of sterol metabolism: Evidence from analysis of Cyp27a1-/- mouse brain and plasma. Molecular Therapy - Nucleic Acids. Nov 1 2018 [PMID: 30471425] (In vitro, Mouse) In vitro Mouse
Mohan V, Radha V, Nguyen TT, Stawiski EW. Comprehensive genomic analysis identifies pathogenic variants in maturity-onset diabetes of the young (MODY) patients in South India. BMC Med. Genet. 2018 Feb 13 [PMID: 29439679] (WB, Human) WB Human
Kropp PA, Dunn JC, Carboneau BA et al. Cooperative function of Pdx1 and Oc1 in multipotent pancreatic progenitors impacts postnatal islet maturation and adaptability. American Journal of Physiology Endocrinology and Metabolism 2017 Dec 12 [PMID: 29351489] (Mouse) Mouse
Jason Spaeth, Manisha Gupte, Mark Perelis et al. Defining a Novel Role for the PDX1 Transcription Factor in Islet Cell Maturation and Proliferation During Weaning. American Diabetes Association 2017 Jul 03 [PMID: 28705881] (ICC/IF, Mouse) ICC/IF Mouse
Theofilopoulos S, Griffiths WJ, Crick PJ et al. Cholestenoic acids regulate motor neuron survival via liver X receptors. J. Clin. Invest. 2014 Nov 03 [PMID: 25271621] (ICC/IF, Mouse)

Details:
NKX6.1 antibody used for ICC-IF on primary midbrain cultures (mouse E11.5) treated or not with 3beta-7alpha-diHCA, 3 betaH,7O-CA, 3 beta,7 beta-diHCA or 3beta-HCA - 4% PFA fixation, 1 hour bocking with 5% normal goat serum/PBS, primary incubated for ON in PBSpH 7.4 containing 0.3% Triton X-100 and 1% BSA (Figure 4 B).
ICC/IF Mouse
Theofilopoulos S, Wang Y, Kitambi SS et al. Brain endogenous liver X receptor ligands selectively promote midbrain neurogenesis Nat Chem Biol 2012 Dec 23 [PMID: 23292650] (ICC/IF, Mouse) ICC/IF Mouse

Reviews for NKX6.1 Antibody (NBP1-49672) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NBP1-49672:
Filter by Applications
IHC-P
(3)
IF
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(2)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin NKX6.1 NBP1-49672
Enlarge
reviewed by:
Jonatan Dereke
IHC-P Rat 04/29/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedPancreas tissue
SpeciesRat
LotD-4

Comments

CommentsWe used a 1:1600 dilution for our assays.
Immunohistochemistry-Paraffin NKX6.1 NBP1-49672
Enlarge
reviewed by:
Anonymous
IHC-P Mouse 01/08/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Pancreas
SpeciesMouse
LotC-1
Immunofluorescence NKX6.1 NBP1-49672
Enlarge
reviewed by:
Anonymous
IF Mouse 10/30/2015
View

Summary

ApplicationImmunofluorescence
SpeciesMouse
FileView PDF
Immunohistochemistry-Paraffin NKX6.1 NBP1-49672
Enlarge
reviewed by:
Anonymous
IHC-P Human 09/24/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman fetal pancreas
SpeciesHuman
LotB-1

Blocking

Blocking DetailsBSA 2%

Primary Anitbody

Dilution Ratio1/100 dilution. Incubation for 1 hour RT.

Secondary Antibody

Secondary DescriptionAlexa fluor 488 anti-rabbit
Secondary Concentration1/500

Details

Fixation DetailsThe sample was fixed in neutral formalin and paraffin-embedded. Perform antigen retrieval low pH (citrate buffer).

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NKX6.1 Antibody (NBP1-49672). (Showing 1 - 1 of 1 FAQs).

  1. Has this antibody been tested on cryo sections for immunohistochemistry?
    • Unfortunately the antibody has never been tested with any frozen section (so called IHC-Fr). This antibody had been tested, in lab, for using in WB, ICC/IF, and IHC-P as these were already posted on the web. Our company use "IHC" just for a faster indexing/searching or browsing the webpages. In theory, when an antibody product can be used in IHC-P, usually it can be used in IHC-Fr with the same dilutions; but not the way around. Therefore you could consider to include NBP1-49672 for using in your ongoing IHC-Fr experiments.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-49672AF350
Alexa Fluor 405 NBP1-49672AF405
Alexa Fluor 488 NBP1-49672AF488
Alexa Fluor 532 NBP1-49672AF532
Alexa Fluor 594 NBP1-49672AF594
Alexa Fluor 647 NBP1-49672AF647
Alexa Fluor 700 NBP1-49672AF700
Alexa Fluor 750 NBP1-49672AF750
Biotin NBP1-49672B
DyLight 350 NBP1-49672UV
DyLight 405 NBP1-49672V
DyLight 488 NBP1-49672G
DyLight 550 NBP1-49672R
DyLight 594 NBP1-49672DL594
DyLight 650 NBP1-49672C
DyLight 680 NBP1-49672FR
DyLight 755 NBP1-49672IR
FITC NBP1-49672F
HRP NBP1-49672H
Janelia Fluor 549 NBP1-49672JF549
Janelia Fluor 646 NBP1-49672JF646

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Bioinformatics Tool for NKX6.1 Antibody (NBP1-49672)

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Blogs on NKX6.1

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Contact Information

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Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

Jonatan Dereke
04/29/2020
Application: IHC-P
Species: Rat

Anonymous
01/08/2019
Application: IHC-P
Species: Mouse

Anonymous
10/30/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol NKX6-1
Entrez
Uniprot
COVID-19 update