Pax2 Antibody [Unconjugated]

Western blot shows lysates of mouse fetal kidney. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Pax2 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Pax2 was detected in immersion fixed BG01V human embryonic stem cells differentiated into the early otic lineage using Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Pax2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Pax2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Pax2
  Asp229-Pro363
  Accession # Q02962
• Specificity: Detects human Pax2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PAX2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pax2 Antibody [Unconjugated]

• paired box 2
• paired box gene 2
• paired box homeotic gene 2
• paired box protein Pax-2
• Pax2

Background

Pax2 is a 40-45 kDa protein belonging to the small but developmentally important family of transcription regulators. Human Pax2 is a 416 amino acid (aa) residue protein with an N-terminal 128 aa DNA-binding paired box domain, a centrally-located octapeptide motif and a C-terminal truncated homeodomain. Based on the presence of the structural domains, Pax2 belongs to subgroup 2 in the Pax family. Pax2 is important for stem cell survival and lineage commitment during development. Pax2 is also expressed in various carcinomas where it seems to mediate anti-apoptotic functions. At least five splice isoforms of human Pax2 have been described within the region used as immunogen (shared by all isoforms). Human and mouse Pax2 share 98% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pax2 Antibody (AF3364)(13)

Publications using AF3364

• T Ishibashi, D Sueto, Y Yoshikawa, K Koga, K Yamaura, M Tsuda Identification of Spinal Inhibitory Interneurons Required for Attenuating Effect of Duloxetine on Neuropathic Allodynia-like Signs in Rats Cells, 2022;11(24):. 2022 [PMID: 36552814] (IHC, Rat)
• F Aby, LE Lorenzo, Z Grivet, R Bouali-Ben, H Martin, S Valerio, S Whitestone, D Isabel, W Idi, O Bouchatta, P De Deurwae, AG Godin, C Herry, X Fioramonti, M Landry, Y De Koninck, P Fossat Switch of serotonergic descending inhibition into facilitation by a spinal chloride imbalance in neuropathic pain Science Advances, 2022;8(30):eabo0689. 2022 [PMID: 35895817] (IHC, Mouse)
• NM Bérenger-C, M Mircea, E Adegeest, PR van den Be, M Feliksik, M Hochane, T Idema, SJ Tans, S Semrau A gastruloid model of the interaction between embryonic and extra-embryonic cell types Journal of tissue engineering, 2022;13(0):2041731422110. 2022 [PMID: 35707767] (IHC, Mouse)
• Y Lan, KM Banks, H Pan, N Verma, GR Dixon, T Zhou, B Ding, O Elemento, S Chen, D Huangfu, T Evans Stage-specific regulation of DNA methylation by TET enzymes during human cardiac differentiation Cell Reports, 2021;37(10):110095. 2021 [PMID: 34879277] (Western Blot, Human)
• EA Paris, JM Bahr, P Bitterman, S Basu, JS Abramowicz, A Barua Incidence of malignant transformation in the oviductal fimbria in laying hens, a preclinical model of spontaneous ovarian cancer PLoS ONE, 2021;16(7):e0255007. 2021 [PMID: 34314463] (IHC, Human)
• RR Das Gupta, L Scheurer, P Pelczar, H Wildner, HU Zeilhofer Neuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neurons Scientific Reports, 2021;11(1):5232. 2021 [PMID: 33664406] (IHC, Mouse)
• RB Roome, FB Bourojeni, B Mona, S Rastegar-P, R Blain, A Dumouchel, C Salesse, WS Thompson, M Brookbank, Y Gitton, L Tessarollo, M Goulding, JE Johnson, M Kmita, A Chédotal, A Kania Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans Cell Rep, 2020;33(8):108425. 2020 [PMID: 33238113] (IHC, Rat)
• A Escalante, R Klein Spinal Inhibitory Ptf1a-Derived Neurons Prevent Self-Generated Itch Cell Rep, 2020;33(8):108422. 2020 [PMID: 33238109] (IHC, Mouse)
• TS Nelson, BK Taylor Targeting spinal neuropeptide Y1 receptor-expressing interneurons to alleviate chronic pain and itch Prog. Neurobiol., 2020;0(0):101894. 2020 [PMID: 32777329] (IHC, Rat)
• Zaitoun I, Wintheiser C, Jamali N, Wang S, Suscha A, Darjatmoko S, Schleck K, Hanna B, Lindner V, Sheibani N, Sorenson C Bcl-2 Expression in Pericytes and Astrocytes Impacts Vascular Development and Homeostasis. Sci Rep, 0;9(1):9700. 0 [PMID: 31273232] (IHC, Mouse)
• IS Zaitoun, CM Wintheiser, N Jamali, S Wang, A Suscha, SR Darjatmoko, K Schleck, BA Hanna, V Lindner, N Sheibani, CM Sorenson Bcl-2 Expression in Pericytes and Astrocytes Impacts Vascular Development and Homeostasis Sci Rep, 2019;9(1):9700. 2019 [PMID: 31273232] (IHC, Mouse)
• TS Nelson, W Fu, RR Donahue, GF Corder, T Hökfelt, RG Wiley, BK Taylor Facilitation of neuropathic pain by the NPY Y1 receptor-expressing subpopulation of excitatory interneurons in the dorsal horn Sci Rep, 2019;9(1):7248. 2019 [PMID: 31076578] (IHC-Fr, Rat)
• Tiwari , Sarika, Dharmarajan , Subraman, Shivanna , Mahesh, Otteson , Deborah, Belecky-Adams , Teri L Histone deacetylase expression patterns in developing murine optic nerve. BMC Dev Biol, 2014;14(0):30. 2014 [PMID: 25011550] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: PAX2
• Uniprot:
  • Q02962()