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Pax2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse fetal kidney. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) followed by HRP-conjugated Anti-Goat IgG ...read more
Pax2 was detected in immersion fixed BG01V human embryonic stem cells differentiated into the early otic lineage using Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) at 10 µg/mL ...read more
Pax2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) at 1 µg/mL for 1 hour at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Pax2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Pax2
Asp229-Pro363
Accession # Q02962
Specificity
Detects human Pax2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PAX2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF3364 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pax2 Antibody [Unconjugated]

  • paired box 2
  • paired box gene 2
  • paired box homeotic gene 2
  • paired box protein Pax-2
  • Pax2

Background

Pax2 is a 40-45 kDa protein belonging to the small but developmentally important family of transcription regulators. Human Pax2 is a 416 amino acid (aa) residue protein with an N-terminal 128 aa DNA-binding paired box domain, a centrally-located octapeptide motif and a C-terminal truncated homeodomain. Based on the presence of the structural domains, Pax2 belongs to subgroup 2 in the Pax family. Pax2 is important for stem cell survival and lineage commitment during development. Pax2 is also expressed in various carcinomas where it seems to mediate anti-apoptotic functions. At least five splice isoforms of human Pax2 have been described within the region used as immunogen (shared by all isoforms). Human and mouse Pax2 share 98% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pax2 Antibody (AF3364)(24)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, IHC-Fr, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(1)
IHC
(10)
IHC-Fr
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(7)
Rat
(4)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using AF3364 Applications Species
Cristina Martinez-Fernandez Martinez-Fernandez de la Camara, Tina Storm, Ahmed Salman, Thomas Burgoyne, Martin Qvist Rasmussen, Harry O. Orlans et al. Developmental Expression of the Cell Cycle Regulator p16INK4a in Retinal Glial Cells: A Novel Marker for Immature Ocular Astrocytes? Journal of Histochemistry & Cytochemistry 6/23/2023 [PMID: 37350564]
Zeng B, Liu Z, Lu Y et al. The single-cell and spatial transcriptional landscape of human gastrulation and early brain development Cell stem cell 5/12/2023 [PMID: 37192616]
Keisuke Koga, Kenta Kobayashi, Makoto Tsuda, Kazufumi Kubota, Yutaka Kitano, Hidemasa Furue Voltage-gated calcium channel subunit alpha 2δ-1 in spinal dorsal horn neurons contributes to aberrant excitatory synaptic transmission and mechanical hypersensitivity after peripheral nerve injury Frontiers in Molecular Neuroscience 3/23/2023 [PMID: 37033377]
N Frezel, M Ranucci, E Foster, H Wende, P Pelczar, R Mendes, RP Ganley, K Werynska, S d'Aquin, C Beccarini, C Birchmeier, HU Zeilhofer, H Wildner c-Maf-positive spinal cord neurons are critical elements of a dorsal horn circuit for mechanical hypersensitivity in neuropathy Cell Reports, 2023-03-21;42(4):112295. 2023-03-21 [PMID: 36947543] (IHC, Mouse) IHC Mouse
T Ishibashi, D Sueto, Y Yoshikawa, K Koga, K Yamaura, M Tsuda Identification of Spinal Inhibitory Interneurons Required for Attenuating Effect of Duloxetine on Neuropathic Allodynia-like Signs in Rats Cells, 2022-12-14;11(24):. 2022-12-14 [PMID: 36552814] (IHC, Rat) IHC Rat
Gioele W. Albisetti, Robert P. Ganley, Francesca Pietrafesa, Karolina Werynska, Marília Magalhaes de Sousa, Rebecca Sipione et al. Inhibitory Kcnip2 neurons of the spinal dorsal horn control behavioral sensitivity to environmental cold Neuron 1/4/2023 [PMID: 36323322]
F Aby, LE Lorenzo, Z Grivet, R Bouali-Ben, H Martin, S Valerio, S Whitestone, D Isabel, W Idi, O Bouchatta, P De Deurwae, AG Godin, C Herry, X Fioramonti, M Landry, Y De Koninck, P Fossat Switch of serotonergic descending inhibition into facilitation by a spinal chloride imbalance in neuropathic pain Science Advances, 2022-07-27;8(30):eabo0689. 2022-07-27 [PMID: 35895817] (IHC, Mouse) IHC Mouse
Tadayuki Ishibashi, Yu Yoshikawa, Daichi Sueto, Ryoichi Tashima, Hidetoshi Tozaki-Saitoh, Keisuke Koga et al. Selective Involvement of a Subset of Spinal Dorsal Horn Neurons Operated by a Prodynorphin Promoter in A beta Fiber-Mediated Neuropathic Allodynia-Like Behavioral Responses in Rats Frontiers in Molecular Neuroscience 6/23/2022 [PMID: 35813063]
NM Bérenger-C, M Mircea, E Adegeest, PR van den Be, M Feliksik, M Hochane, T Idema, SJ Tans, S Semrau A gastruloid model of the interaction between embryonic and extra-embryonic cell types Journal of tissue engineering, 2022-06-11;13(0):2041731422110. 2022-06-11 [PMID: 35707767] (IHC, Mouse) IHC Mouse
Ines Serra, Ana Stravs, Catarina Osório, Maria Roa Oyaga, Martijn Schonewille, Christian Tudorache et al. Tsc1 Haploinsufficiency Leads to Pax2 Dysregulation in the Developing Murine Cerebellum Frontiers in Molecular Neuroscience 5/13/2022 [PMID: 35645731]
Show All 24 Publications.

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Bioinformatics

Gene Symbol PAX2
Uniprot