Pax2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) followed ...read more
Pax2 was detected in immersion fixed BG01V human embryonic stem cells differentiated into the early otic lineage using Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) at 10 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Pax2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Pax2
Asp229-Pro363
Accession # Q02962
Specificity
Detects human Pax2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PAX2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF3364 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pax2 Antibody [Unconjugated]

  • paired box 2
  • paired box gene 2
  • paired box homeotic gene 2
  • paired box protein Pax-2
  • Pax2

Background

Pax2 is a 40-45 kDa protein belonging to the small but developmentally important family of transcription regulators. Human Pax2 is a 416 amino acid (aa) residue protein with an N-terminal 128 aa DNA-binding paired box domain, a centrally-located octapeptide motif and a C-terminal truncated homeodomain. Based on the presence of the structural domains, Pax2 belongs to subgroup 2 in the Pax family. Pax2 is important for stem cell survival and lineage commitment during development. Pax2 is also expressed in various carcinomas where it seems to mediate anti-apoptotic functions. At least five splice isoforms of human Pax2 have been described within the region used as immunogen (shared by all isoforms). Human and mouse Pax2 share 98% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pax2 Antibody (AF3364)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: IHC, IHC-Fr, Western Blot.


Filter By Application
IHC
(6)
IHC-Fr
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(5)
Rat
(2)
All Species
Showing Publications 1 - 9 of 9.
Publications using AF3364 Applications Species
Y Lan, KM Banks, H Pan, N Verma, GR Dixon, T Zhou, B Ding, O Elemento, S Chen, D Huangfu, T Evans Stage-specific regulation of DNA methylation by TET enzymes during human cardiac differentiation Cell Reports, 2021;37(10):110095. 2021 [PMID: 34879277] (Western Blot, Human) Western Blot Human
EA Paris, JM Bahr, P Bitterman, S Basu, JS Abramowicz, A Barua Incidence of malignant transformation in the oviductal fimbria in laying hens, a preclinical model of spontaneous ovarian cancer PLoS ONE, 2021;16(7):e0255007. 2021 [PMID: 34314463] (IHC, Human) IHC Human
RR Das Gupta, L Scheurer, P Pelczar, H Wildner, HU Zeilhofer Neuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neurons Scientific Reports, 2021;11(1):5232. 2021 [PMID: 33664406] (IHC, Mouse) IHC Mouse
RB Roome, FB Bourojeni, B Mona, S Rastegar-P, R Blain, A Dumouchel, C Salesse, WS Thompson, M Brookbank, Y Gitton, L Tessarollo, M Goulding, JE Johnson, M Kmita, A Chédotal, A Kania Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans Cell Rep, 2020;33(8):108425. 2020 [PMID: 33238113] (IHC, Rat) IHC Rat
A Escalante, R Klein Spinal Inhibitory Ptf1a-Derived Neurons Prevent Self-Generated Itch Cell Rep, 2020;33(8):108422. 2020 [PMID: 33238109] (IHC, Mouse) IHC Mouse
Zaitoun I, Wintheiser C, Jamali N, Wang S, Suscha A, Darjatmoko S, Schleck K, Hanna B, Lindner V, Sheibani N, Sorenson C Bcl-2 Expression in Pericytes and Astrocytes Impacts Vascular Development and Homeostasis. Sci Rep, 0;9(1):9700. 0 [PMID: 31273232] (IHC, Mouse) IHC Mouse
IS Zaitoun, CM Wintheiser, N Jamali, S Wang, A Suscha, SR Darjatmoko, K Schleck, BA Hanna, V Lindner, N Sheibani, CM Sorenson Bcl-2 Expression in Pericytes and Astrocytes Impacts Vascular Development and Homeostasis Sci Rep, 2019;9(1):9700. 2019 [PMID: 31273232] (IHC, Mouse) IHC Mouse
TS Nelson, W Fu, RR Donahue, GF Corder, T Hökfelt, RG Wiley, BK Taylor Facilitation of neuropathic pain by the NPY Y1 receptor-expressing subpopulation of excitatory interneurons in the dorsal horn Sci Rep, 2019;9(1):7248. 2019 [PMID: 31076578] (IHC-Fr, Rat) IHC-Fr Rat
Tiwari , Sarika, Dharmarajan , Subraman, Shivanna , Mahesh, Otteson , Deborah, Belecky-Adams , Teri L Histone deacetylase expression patterns in developing murine optic nerve. BMC Dev Biol, 2014;14(0):30. 2014 [PMID: 25011550] (IHC-Fr, Mouse) IHC-Fr Mouse

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Bioinformatics

Gene Symbol PAX2
Uniprot