Neurogenin-3 Antibody [Unconjugated]


Western blot shows lysates of human placenta and prostate tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Neurogenin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3444) followed by more
Neurogenin‑3 was detected in immersion fixed paraffin-embedded sections of human placenta using Sheep Anti-Human Neurogenin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3444) at more

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Neurogenin-3 Antibody [Unconjugated] Summary

E. coli-derived recombinant human Neurogenin-3
Accession # Q9Y4Z2
Detects human Neurogenin-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Neurogenin-3 is observed, and less than 1% cross-reactivity with recombinant human Neurogenin-1 is observed.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 1 ug/mL
Read Publications using
AF3444 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neurogenin-3 Antibody [Unconjugated]

  • Atoh5
  • BHLHA7
  • bHLHa7NGN-3
  • Class A basic helix-loop-helix protein 7
  • Math4B
  • neurogenin 3
  • Neurogenin3
  • Neurogenin-3
  • NGN3
  • Protein atonal homolog 5
  • Relax


Neurogenin-3 (NGN3; also Protein atonal homolog 5) is a 23 kDa, developmentally expressed nuclear protein that belongs to a family of atonal-related basic helix‑loop‑helix (bHLH) transcription factors. bHLH proteins typically form dimers when binding to DNA. NGN3 expression is required for the lineage differentiation of all endocrine cell types in the pancreas, including the formation of islet beta  cells. NGN3 is also neurogenic, controlling the number of dendrites generated by hippocampal neurons. Human NGN3 is 214 amino acids (aa) in length. It contains a DNA binding region (aa 84‑95) plus an HLH motif (aa 96‑136). Over aa 3‑214, human NGN3 shares 76% and 84% aa identity with mouse and canine NGN3, respectively.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: ICC, IHC, Simple Western, WB
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
Species: Hu, Mu
Applications: ICC, WB
Species: Hu
Applications: IHC, IHC-P, WB
Species: Hu, Mu
Applications: CyTOF-ready, ICC, ICFlow
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, Simple Western, WB
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, IF, IHC, IHC-P, WB
Species: Hu
Applications: ICC, WB
Species: Fe, Hu, Mu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, KO, WB
Species: Ca, Hu, Pm
Applications: IHC, IHC-P, WB
Species: Hu
Applications: ICC, IHC, Simple Western, WB
Species: Fi, Hu, Pm, Mu, Pm, Rt, Sh
Applications: ICC/IF, IHC, IP, WB
Species: Hu
Applications: ChIP, ICC, IHC, WB
Species: Hu, Pm, Mu, Rt
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA
Species: Hu, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: CyTOF-reported, ICC, ICFlow
Species: Hu
Applications: WB
Species: Hu
Applications: WB, IHC

Publications for Neurogenin-3 Antibody (AF3444)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Flow Cytometry, ICC, IHC-P, Western Blot.

Filter By Application
Flow Cytometry
Western Blot
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 10.
Publications using AF3444 Applications Species
NJ Hogrebe, KG Maxwell, P Augsornwor, JR Millman Generation of insulin-producing pancreatic beta cells from multiple human stem cell lines Nature Protocols, 2021-08-04;0(0):. 2021-08-04 [PMID: 34349281] (Flow Cytometry, ICC, Human) Flow Cytometry, ICC Human
NK Vinckier, NA Patel, RJ Geusz, A Wang, J Wang, I Matta, AR Harrington, M Wortham, N Wetton, J Wang, US Jhala, MG Rosenfeld, CW Benner, HP Shih, M Sander LSD1-mediated enhancer silencing attenuates retinoic acid signalling during pancreatic endocrine cell development Nat Commun, 2020-04-29;11(1):2082. 2020-04-29 [PMID: 32350257] (ICC, Human, Mouse) ICC Human, Mouse
JR Alvarez-Do, J Donaghey, N Rasouli, JHR Kenty, A Helman, J Charlton, JR Straubhaar, A Meissner, DA Melton Circadian Entrainment Triggers Maturation of Human In�Vitro Islets Cell Stem Cell, 2019-12-12;26(1):108-122.e10. 2019-12-12 [PMID: 31839570] (ICC, Human) ICC Human
N Sharon, J Vanderhoof, J Straubhaar, J Mueller, R Chawla, Q Zhou, EN Engquist, C Trapnell, DK Gifford, DA Melton Wnt Signaling Separates the Progenitor and Endocrine Compartments during Pancreas Development Cell Rep, 2019-05-21;27(8):2281-2291.e5. 2019-05-21 [PMID: 31116975] (Flow Cytometry, Human) Flow Cytometry Human
EA Rosado-Oli, K Anderson, JH Kenty, DA Melton YAP inhibition enhances the differentiation of functional stem cell-derived insulin-producing ? cells Nat Commun, 2019-04-01;10(1):1464. 2019-04-01 [PMID: 30931946] (Flow Cytometry, ICC, Human) Flow Cytometry, ICC Human
II Aigha, B Memon, AK Elsayed, EM Abdelalim Differentiation of human pluripotent stem cells into two distinct NKX6.1 populations of pancreatic progenitors Stem Cell Res Ther, 2018-04-03;9(1):83. 2018-04-03 [PMID: 29615106] (ICC, Human) ICC Human
NAJ Krentz, D van Hoof, Z Li, A Watanabe, M Tang, C Nian, MS German, FC Lynn Phosphorylation of NEUROG3 Links Endocrine Differentiation to the Cell Cycle in Pancreatic Progenitors Dev. Cell, 2017-04-24;41(2):129-142.e6. 2017-04-24 [PMID: 28441528] (Western Blot, Human) Western Blot Human
J Saarimäki-, D Balboa, MA Russell, J Saarikettu, M Kinnunen, S Keskitalo, A Malhi, C Valensisi, C Andrus, S Eurola, H Grym, J Ustinov, K Wartiovaar, RD Hawkins, O Silvennoin, M Varjosalo, NG Morgan, T Otonkoski An Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation Cell Rep, 2017-04-11;19(2):281-294. 2017-04-11 [PMID: 28402852] (ICC, Human) ICC Human
Cai , Qing, Bonfanti , Paola, Sambathkumar , Rangaraj, Vanuytsel , Kim, Vanhove , Jolien, Gysemans , Conny, Debiec-Rychter , Maria, Raitano , Susanna, Heimberg , Harry, Ordovas , Laura, Verfaillie , Catherin Prospectively isolated NGN3-expressing progenitors from human embryonic stem cells give rise to pancreatic endocrine cells. Stem Cells Transl Med, 2014-02-03;3(4):489-99. 2014-02-03 [PMID: 24493854] (ICC, Human) ICC Human
Kayali AG, Lopez AD, Hao E, Hinton A, Hayek A, King CC The SDF-1α/CXCR4 Axis is Required for Proliferation and Maturation of Human Fetal Pancreatic Endocrine Progenitor Cells. PLoS ONE, 2012-06-22;7(6):e38721. 2012-06-22 [PMID: 22761699] (IHC-P, Human) IHC-P Human

Reviews for Neurogenin-3 Antibody (AF3444) (0)

There are no reviews for Neurogenin-3 Antibody (AF3444). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Neurogenin-3 Antibody (AF3444) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional Neurogenin-3 Products

Bioinformatics Tool for Neurogenin-3 Antibody (AF3444)

Discover related pathways, diseases and genes to Neurogenin-3 Antibody (AF3444). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Neurogenin-3 Antibody (AF3444)

Discover more about diseases related to Neurogenin-3 Antibody (AF3444).

Pathways for Neurogenin-3 Antibody (AF3444)

View related products by pathway.

PTMs for Neurogenin-3 Antibody (AF3444)

Learn more about PTMs related to Neurogenin-3 Antibody (AF3444).

Blogs on Neurogenin-3

There are no specific blogs for Neurogenin-3, but you can read our latest blog posts.
mFlour Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Neurogenin-3 Antibody [Unconjugated] and receive a gift card or discount.


Gene Symbol NEUROG3