Neurogenin-3 Antibody [Unconjugated]

Western blot shows lysates of human placenta and prostate tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Neurogenin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3444) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neurogenin‑3 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Neurogenin‑3 was detected in immersion fixed paraffin-embedded sections of human placenta using Sheep Anti-Human Neurogenin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3444) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell nuclei in decidual cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Neurogenin-3 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Neurogenin-3
  Met1-Leu214
  Accession # Q9Y4Z2
• Specificity: Detects human Neurogenin-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Neurogenin-3 is observed, and less than 1% cross-reactivity with recombinant human Neurogenin-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: NEUROG3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neurogenin-3 Antibody [Unconjugated]

• Atoh5
• BHLHA7
• bHLHa7NGN-3
• Class A basic helix-loop-helix protein 7
• Math4B
• NEUROG3
• neurogenin 3
• Neurogenin3
• Neurogenin-3
• NGN3
• Protein atonal homolog 5
• Relax

Background

Neurogenin-3 (NGN3; also Protein atonal homolog 5) is a 23 kDa, developmentally expressed nuclear protein that belongs to a family of atonal-related basic helix‑loop‑helix (bHLH) transcription factors. bHLH proteins typically form dimers when binding to DNA. NGN3 expression is required for the lineage differentiation of all endocrine cell types in the pancreas, including the formation of islet beta  cells. NGN3 is also neurogenic, controlling the number of dendrites generated by hippocampal neurons. Human NGN3 is 214 amino acids (aa) in length. It contains a DNA binding region (aa 84‑95) plus an HLH motif (aa 96‑136). Over aa 3‑214, human NGN3 shares 76% and 84% aa identity with mouse and canine NGN3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neurogenin-3 Antibody (AF3444)(10)

Publications using AF3444

• NJ Hogrebe, KG Maxwell, P Augsornwor, JR Millman Generation of insulin-producing pancreatic beta cells from multiple human stem cell lines Nature Protocols, 2021-08-04;0(0):. 2021-08-04 [PMID: 34349281] (Flow Cytometry, ICC, Human)
• NK Vinckier, NA Patel, RJ Geusz, A Wang, J Wang, I Matta, AR Harrington, M Wortham, N Wetton, J Wang, US Jhala, MG Rosenfeld, CW Benner, HP Shih, M Sander LSD1-mediated enhancer silencing attenuates retinoic acid signalling during pancreatic endocrine cell development Nat Commun, 2020-04-29;11(1):2082. 2020-04-29 [PMID: 32350257] (ICC, Human, Mouse)
• JR Alvarez-Do, J Donaghey, N Rasouli, JHR Kenty, A Helman, J Charlton, JR Straubhaar, A Meissner, DA Melton Circadian Entrainment Triggers Maturation of Human In�Vitro Islets Cell Stem Cell, 2019-12-12;26(1):108-122.e10. 2019-12-12 [PMID: 31839570] (ICC, Human)
• N Sharon, J Vanderhoof, J Straubhaar, J Mueller, R Chawla, Q Zhou, EN Engquist, C Trapnell, DK Gifford, DA Melton Wnt Signaling Separates the Progenitor and Endocrine Compartments during Pancreas Development Cell Rep, 2019-05-21;27(8):2281-2291.e5. 2019-05-21 [PMID: 31116975] (Flow Cytometry, Human)
• EA Rosado-Oli, K Anderson, JH Kenty, DA Melton YAP inhibition enhances the differentiation of functional stem cell-derived insulin-producing ? cells Nat Commun, 2019-04-01;10(1):1464. 2019-04-01 [PMID: 30931946] (Flow Cytometry, ICC, Human)
• II Aigha, B Memon, AK Elsayed, EM Abdelalim Differentiation of human pluripotent stem cells into two distinct NKX6.1 populations of pancreatic progenitors Stem Cell Res Ther, 2018-04-03;9(1):83. 2018-04-03 [PMID: 29615106] (ICC, Human)
• NAJ Krentz, D van Hoof, Z Li, A Watanabe, M Tang, C Nian, MS German, FC Lynn Phosphorylation of NEUROG3 Links Endocrine Differentiation to the Cell Cycle in Pancreatic Progenitors Dev. Cell, 2017-04-24;41(2):129-142.e6. 2017-04-24 [PMID: 28441528] (Western Blot, Human)
• J Saarimäki-, D Balboa, MA Russell, J Saarikettu, M Kinnunen, S Keskitalo, A Malhi, C Valensisi, C Andrus, S Eurola, H Grym, J Ustinov, K Wartiovaar, RD Hawkins, O Silvennoin, M Varjosalo, NG Morgan, T Otonkoski An Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation Cell Rep, 2017-04-11;19(2):281-294. 2017-04-11 [PMID: 28402852] (ICC, Human)
• Cai , Qing, Bonfanti , Paola, Sambathkumar , Rangaraj, Vanuytsel , Kim, Vanhove , Jolien, Gysemans , Conny, Debiec-Rychter , Maria, Raitano , Susanna, Heimberg , Harry, Ordovas , Laura, Verfaillie , Catherin Prospectively isolated NGN3-expressing progenitors from human embryonic stem cells give rise to pancreatic endocrine cells. Stem Cells Transl Med, 2014-02-03;3(4):489-99. 2014-02-03 [PMID: 24493854] (ICC, Human)
• Kayali AG, Lopez AD, Hao E, Hinton A, Hayek A, King CC The SDF-1α/CXCR4 Axis is Required for Proliferation and Maturation of Human Fetal Pancreatic Endocrine Progenitor Cells. PLoS ONE, 2012-06-22;7(6):e38721. 2012-06-22 [PMID: 22761699] (IHC-P, Human)

Bioinformatics

• Gene Symbol: NEUROG3
• Uniprot:
  • Q9Y4Z2()