Neurogenin-3 Antibody

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Western blot shows lysates of human placenta and prostate tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Neurogenin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3444) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Neurogenin-3 Antibody Summary

Immunogen
E. coli-derived recombinant human Neurogenin-3
Met1-Leu214
Accession # Q9Y4Z2
Specificity
Detects human Neurogenin-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Neurogenin-3 is observed, and less than 1% cross-reactivity with recombinant human Neurogenin-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
NEUROG3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF3444 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neurogenin-3 Antibody

  • Atoh5
  • BHLHA7
  • bHLHa7NGN-3
  • Class A basic helix-loop-helix protein 7
  • Math4B
  • NEUROG3
  • neurogenin 3
  • Neurogenin3
  • Neurogenin-3
  • NGN3
  • Protein atonal homolog 5
  • Relax

Background

Neurogenin-3 (NGN3; also Protein atonal homolog 5) is a 23 kDa, developmentally expressed nuclear protein that belongs to a family of atonal-related basic helix‑loop‑helix (bHLH) transcription factors. bHLH proteins typically form dimers when binding to DNA. NGN3 expression is required for the lineage differentiation of all endocrine cell types in the pancreas, including the formation of islet beta  cells. NGN3 is also neurogenic, controlling the number of dendrites generated by hippocampal neurons. Human NGN3 is 214 amino acids (aa) in length. It contains a DNA binding region (aa 84‑95) plus an HLH motif (aa 96‑136). Over aa 3‑214, human NGN3 shares 76% and 84% aa identity with mouse and canine NGN3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neurogenin-3 Antibody (AF3444)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC, IHC Paraffin-embedded, WB.


Filter By Application
ICC
(3)
IHC Paraffin-embedded
(1)
WB
(1)
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Filter By Species
Human
(5)
All Species
Showing Publications 1 - 5 of 5.
Publications using AF3444 Applications Species
II Aigha, B Memon, AK Elsayed, EM Abdelalim Differentiation of human pluripotent stem cells into two distinct NKX6.1 populations of pancreatic progenitors Stem Cell Res Ther, 2018;9(1):83. 2018 [PMID: 29615106] (ICC,, Human) ICC Human
NAJ Krentz, D van Hoof, Z Li, A Watanabe, M Tang, C Nian, MS German, FC Lynn Phosphorylation of NEUROG3 Links Endocrine Differentiation to the Cell Cycle in Pancreatic Progenitors Dev. Cell, 2017;41(2):129-142.e6. 2017 [PMID: 28441528] (WB,, Human) WB Human
J Saarimäki-, D Balboa, MA Russell, J Saarikettu, M Kinnunen, S Keskitalo, A Malhi, C Valensisi, C Andrus, S Eurola, H Grym, J Ustinov, K Wartiovaar, RD Hawkins, O Silvennoin, M Varjosalo, NG Morgan, T Otonkoski An Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation Cell Rep, 2017;19(2):281-294. 2017 [PMID: 28402852] (ICC,, Human) ICC Human
Cai, Qing, Bonfanti, Paola, Sambathkumar, Rangaraj, Vanuytsel, Kim, Vanhove, Jolien, Gysemans, Conny, Debiec-Rychter, Maria, Raitano, Susanna, Heimberg, Harry, Ordovas, Laura, Verfaillie, Catherin Prospectively isolated NGN3-expressing progenitors from human embryonic stem cells give rise to pancreatic endocrine cells. Stem Cells Transl Med, 2014;3(4):489-99. 2014 [PMID: 24493854] (ICC,, Human) ICC Human
Kayali AG, Lopez AD, Hao E, Hinton A, Hayek A, King CC The SDF-1α/CXCR4 Axis is Required for Proliferation and Maturation of Human Fetal Pancreatic Endocrine Progenitor Cells. PLoS ONE, 2012;7(6):e38721. 2012 [PMID: 22761699] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human

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Bioinformatics

Gene Symbol NEUROG3
Entrez
Uniprot