Osteopontin/OPN Antibody (1B20) - Azide and BSA Free

Western Blot: Osteopontin/OPN Antibody (1B20) - Azide and BSA Free [NBP2-80895] - Analysis of Osteopontin expression in U2OS whole cell lysate. Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: Osteopontin/OPN Antibody (1B20) - Azide and BSA Free [NBP2-80895] - Osteopontin antibody was tested at 1:50 in U2OS cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red). Image from the standard format of this antibody.

Immunohistochemistry: Osteopontin/OPN Antibody (1B20) - Azide and BSA Free [NBP2-80895] - Analysis of Osteopontin on human lung adenocarcinoma. Image from the standard format of this antibody.

Simple Western: Osteopontin/OPN Antibody (1B20) - Azide and BSA Free [NBP2-80895] - Simple Western lane view shows a specific band for Osteopontin in 0.5 mg/ml of Human Breast Cancer lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody Image from the standard format of this antibody.

• Reactivity: Hu, Mu, Rt, Rb
• Applications: WB, Simple Western, ICC/IF, IHC, IP
• Clone: 1B20
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Osteopontin/OPN Antibody (1B20) - Azide and BSA Free Summary

• Immunogen: Synthetic peptide corresponding to the C-terminus of human Osteopontin [Swiss-Prot# P10451].
• Localization: Secreted.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 24269728)
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
  • Simple Western 1:20
  • Western Blot 1:1000
• Application Notes: In Western blot multiple bands can be seen due to glycosylation and phosphorylation of the protein.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:20. Separated by Size-Wes, Sally Sue/Peggy Sue.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Osteopontin/OPN Antibody (1B20) - Azide and BSA Free

• BNSP
• Bone sialoprotein 1
• Eta-1
• MGC110940
• Nephropontin
• OPN
• Osteopontin
• secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
• secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
• Spp1
• SPP-1
• SPP1/CALPHA1 fusion
• Urinary stone protein
• uropontin

Background

The matricellular protein osteopontin binds to cell surface receptors and is secreted into many body fluids including milk, blood, and urine, depending on the organ of origin. This makes osteopontin an ideal candidate for being a biomarker as the secreted form is easily obtained in throwaway fluids, and mimics the cellular environment from which it is released. Osteopontin is important in immune responses and inflammation as well as bone generation and remodeling. In autistic children, serum levels of osteopontin are correlated to the severity of disease, probably due to a brain inflammation pattern in these children. In aortic valve sclerosis and stenosis, increased levels of secreted osteopontin are also noted. Osteopontin has also been suggested as a cancer biomarker, since it is associated with tumor formation, progression and metastasis. In bone and tooth formation osteopontin is known to be a negative regulator of parathyroid hormone-related protein receptor, which induces osteogenesis. Without appropriate levels of osteopontin, bone growth continues unregulated, and leads to specific bone cancers. In short, osteopontin is a strong marker for bone growth, inflammation and certain cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPP1