Osteopontin/OPN Antibody (1B20) [Alexa Fluor® 488]

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Product Details

Summary
Reactivity Hu, Rt, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
1B20
Clonality
Monoclonal
Host
Mouse

Osteopontin/OPN Antibody (1B20) [Alexa Fluor® 488] Summary

Immunogen
A synthetic peptide corresponding to the C-terminus of human Osteopontin [Swiss-Prot# P10451].
Localization
Secreted.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SPP1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
Alexa Fluor 488
Format
Alexa Fluor 488 (A=488, E=515-545)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
This Osteopontin (1B20) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In Western blot multiple bands can be seen due to glycosylation and phosphorylation of the protein. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24269728)

Reactivity Notes

Human, Rat and Rabbit.

Alternate Names for Osteopontin/OPN Antibody (1B20) [Alexa Fluor® 488]

  • BNSP
  • Bone sialoprotein 1
  • Eta-1
  • MGC110940
  • Nephropontin
  • OPN
  • osteopontin
  • Osteopontin/OPN
  • secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
  • secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
  • Spp1
  • SPP-1
  • SPP1/CALPHA1 fusion
  • Urinary stone protein
  • uropontin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Osteopontin/OPN Antibody (NB110-89062AF488) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 647 Labeled NB110-89062AF647
Alexa Fluor 700 Labeled NB110-89062AF700

Additional Osteopontin/OPN Products

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol SPP1
Entrez
Uniprot

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