Osteopontin/OPN Antibody (1B20) (NB110-89062)

Images

 
Western Blot: Osteopontin/OPN Antibody (1B20) [NB110-89062] - Analysis of Osteopontin expression in U2OS whole cell lysate.
Immunocytochemistry/ Immunofluorescence: Osteopontin/OPN Antibody (1B20) [NB110-89062] - Osteopontin antibody was tested at 1:50 in U2OS cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and ...read more
Immunohistochemistry: Osteopontin/OPN Antibody (1B20) [NB110-89062] - Analysis of Osteopontin on human lung adenocarcinoma.
Simple Western: Osteopontin/OPN Antibody (1B20) [NB110-89062] - Simple Western lane view shows a specific band for Osteopontin in 0.5 mg/ml of Human Breast Cancer lysate. This experiment was performed under reducing ...read more

Product Details

Summary
Reactivity Hu, Rt, RbSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
1B20
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Osteopontin/OPN Antibody (1B20) Summary

Immunogen
A synthetic peptide corresponding to the C-terminus of human Osteopontin [Swiss-Prot# P10451].
Localization
Secreted.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SPP1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:20
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
This Osteopontin (1B20) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In Western blot multiple bands can be seen due to glycosylation and phosphorylation of the protein. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24269728).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 1 Review rated 5
using
NB110-89062 in the following applications:

Publications
Read Publications using
NB110-89062 in the following applications:

Reactivity Notes

Human, Rat and Rabbit.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS pH 7.4
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Osteopontin/OPN Antibody (1B20)

  • BNSP
  • Bone sialoprotein 1
  • Eta-1
  • MGC110940
  • Nephropontin
  • OPN
  • Osteopontin
  • secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
  • secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
  • Spp1
  • SPP-1
  • SPP1/CALPHA1 fusion
  • Urinary stone protein
  • uropontin

Background

The matricellular protein osteopontin binds to cell surface receptors and is secreted into many body fluids including milk, blood and urine, depending on the organ of origin. This makes osteopontin an ideal candidate for being a biomarker as the secreted form is easily obtained in throwaway fluids, and mimics the cellular environment from which it is released. Osteopontin is important in immune responses and inflammation as well as bone generation and remodeling. In autistic children, serum levels of osteopontin are correlated to the severity of disease, probably due to a brain inflammation pattern in these children. In aortic valve sclerosis and stenosis, increased levels of secreted osteopontin are also noted. Osteopontin has also been suggested as a cancer biomarker, since it is associated with tumor formation, progression and metastasis. In bone and tooth formation osteopontin is known to be a negative regulator of parathyroid hormone-related protein receptor, which induces osteogenesis. Without appropriate levels of osteopontin, bone growth continues unregulated, and leads to specific bone cancers. In short, osteopontin is a strong marker for bone growth, inflammation and certain cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Osteopontin/OPN Antibody (NB110-89062)(16)

We have publications tested in 3 confirmed species: Human, Rat, Rabbit.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
IHC-Fr
(2)
IHC-P
(7)
WB
(4)
All Applications
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Human
(8)
Rat
(2)
Rabbit
(4)
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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB110-89062 Applications Species
Jayash SN, Hashim NM, Misran M, Baharuddin NA. Local application of osteoprotegerin-chitosan gel in critical-sized defects in a rabbit model PeerJ 2017 Jul 04 [PMID: 28674665] (IHC-P, Rabbit) IHC-P Rabbit
Wada S, Kanzaki H, Narimiya T, Nakamura Y. Novel device for application of continuous mechanical tensile strain to mammalian cells. Biol Open. Mar 16 2017 [PMID: 28302667] (WB, Human) WB Human
Wu YT, Su WR, Wu PT et al. Degradation of elastic fiber and elavated elastase expression in long head of biceps tendinopathy. J. Orthop. Res. Dec 9 2016 [PMID: 27935111]
Jayash SN, Hashim NM, Baharuddin MM. In vitro evaluation of osteoprotegerin in chitosan for potential bone defect applications. PeerJ Print. Aug 23 2016 [PMID: 27635307] (WB, Human) WB Human
Cheng MQ, Wahafu T, Jiang GF et al. A novel open-porous magnesium scaffold with controllable microstructures and properties for bone regeneration. Sci Rep. 2016 Apr 13 [PMID: 27071777] (IHC-P, Human) IHC-P Human
Di Bartolomeo M, Pietrantonio F, Pellegrinelli A et al. Osteopontin, E-cadherin, and beta-catenin expression as prognostic biomarkers in patients with radically resected gastric cancer Gastric Cancer 2015 Apr 11 [PMID: 25862567] (IHC-P, Human) IHC-P Human
Magro CM, Momtahen S, Hagen JW. Osteopontin expression in biopsies of calciphylaxis Eur J Dermatol 2015 Feb 01 [PMID: 25847841] (IHC-P, Human)

Details:
Osteopontin/OPN antibody was used for IHC-P application on sections from formalin-fixed paraffin embedded skin biopsies of males and female subjects with a diagnosis of calciphylaxis, which combines features of vascular thrombotic occlusion and endoluminal calcification.
IHC-P Human
Nguyen LT, Min YK, Lee BT. Nanoparticle Biphasic Calcium Phosphate loading on Gelatin-Pectin scaffold for improved bone regeneration Tissue Eng Part A. 2015 Jan 20 [PMID: 25602709] (IHC-P, Rabbit)

Details:
Osteopontin antibody used for Immunohistochemisty on the femur bone samples (collected after 6 and 12 weeks of implantation) from Rabbit.
IHC-P Rabbit
Fujita K, Fukuda M, Fukui H et al. Intratracheal instillation of single-wall carbon nanotubes in the rat lung induces time-dependent changes in gene expression Nanotoxicology. 2014 Jun 09 [PMID: 24911292] (IHC-P, Rat)

Details:
Osteopontin antibody used for IHC-P in paraformaldehyde-fixed lung tissues from Wistar rats intratracheally instilled with vehicle control, L-SWCNT/single-wall carbon nanotubes or H-SWCNT at 90 days post-instillation. Antibody diluted in TBS and target detected using HRP-conjugated biotinylated secondary -DAB (Figure 7).
IHC-P Rat
Goswami R, Millo T, Mishra S et al. Expression of osteogenic molecules in the caudate nucleus and gray matter and their potential relevance for basal ganglia calcification in hypoparathyroidism. J. Clin. Endocrinol. Metab. 2014 Feb 20 [PMID: 24552219] (WB, IHC-Fr, Human) WB, IHC-Fr Human
Show All 16 Publications.

Review for Osteopontin/OPN Antibody (NB110-89062) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ELISA.

Reviews using NB110-89062:
Filter by Applications
ELISA
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Cindy Zhou
ELISA Human 08/11/2017
View

Summary

ApplicationELISA
Sample TestedHuman breast carcinoma, Human breast cancer plasma
SpeciesHuman
LotB-1, B-2, B-3, B-4, B-5, B-6

Comments

CommentsIt will be nice if using PBS buffer for product. Removing Gly-Tris buffer is a pain.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Osteopontin/OPN Antibody (NB110-89062). (Showing 1 - 1 of 1 FAQs).

  1. I am very interested in your anti-human osteopontin (SPP1) (cat #NB110-89062). I work with bovine and would like to use the antibody in the blot. According to your description, the immunogen is a synthetic peptide which binds to the C-terminus of the human. The sequences of the C-termini are very similar in humans and cattle. Could you kindly tell me what amino acids against the C-terminus of the human antibody is directed exactly?
    • The immunogen sequence for NB110-89062 lies within amino acids 284-314 of isoform A, UniProt P10451. I have carried out a UniProt Blast analysis of the exact immunogen sequence versus Bos taurus (bovine) proteins for you, and the result was a 95% match for both osteopontin and osteopontin-K. This is a high percentage strongly suggesting that the antibody will be suitable for bovine, although we cannot guarantee it as this has not been tested in the lab. Because you would be using NB110-89062 with a species not stated on the web page / datasheet for this product, you would not be covered by the Novus Guarantee but you would be eligible for our special Innovator's Reward program, where as a thank you for sharing your results with us (whether the antibody proved suitable or not) by completing a review, we would give you a voucher code for the price of the product. Please contact us at innovators@novusbio.com for more information.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-89062AF405
Alexa Fluor 488 Labeled NB110-89062AF488
Alexa Fluor 647 Labeled NB110-89062AF647
Alexa Fluor 700 Labeled NB110-89062AF700
Biotin Labeled NB110-89062B
DyLight 350 Labeled NB110-89062UV
DyLight 405 Labeled NB110-89062V
DyLight 488 Labeled NB110-89062G
DyLight 550 Labeled NB110-89062R
DyLight 650 Labeled NB110-89062C
DyLight 680 Labeled NB110-89062FR
DyLight 755 Labeled NB110-89062IR
FITC Labeled NB110-89062F
HRP Labeled NB110-89062H

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Recent Reviews

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Cindy Zhou
08/11/2017
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol SPP1
Entrez
Uniprot

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