Western Blot: Osteopontin/OPN Antibody (1B20) [NB110-89062] - Analysis of Osteopontin expression in U2OS whole cell lysate.
Immunocytochemistry/ Immunofluorescence: Osteopontin/OPN Antibody (1B20) [NB110-89062] - Osteopontin antibody was tested at 1:50 in U2OS cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and ...read more
Immunohistochemistry: Osteopontin/OPN Antibody (1B20) [NB110-89062] - Analysis of Osteopontin on human lung adenocarcinoma.
Simple Western: Osteopontin/OPN Antibody (1B20) [NB110-89062] - Simple Western lane view shows a specific band for Osteopontin in 0.5 mg/ml of Human Breast Cancer lysate. This experiment was performed under reducing ...read more
This Osteopontin (1B20) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In Western blot multiple bands can be seen due to glycosylation and phosphorylation of the protein. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24269728).
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Read 1 Review rated 5 using NB110-89062 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS pH 7.4
0.05% Sodium Azide
Protein G purified
Alternate Names for Osteopontin/OPN Antibody (1B20)
Bone sialoprotein 1
secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
Urinary stone protein
The matricellular protein osteopontin binds to cell surface receptors and is secreted into many body fluids including milk, blood and urine, depending on the organ of origin. This makes osteopontin an ideal candidate for being a biomarker as the secreted form is easily obtained in throwaway fluids, and mimics the cellular environment from which it is released. Osteopontin is important in immune responses and inflammation as well as bone generation and remodeling. In autistic children, serum levels of osteopontin are correlated to the severity of disease, probably due to a brain inflammation pattern in these children. In aortic valve sclerosis and stenosis, increased levels of secreted osteopontin are also noted. Osteopontin has also been suggested as a cancer biomarker, since it is associated with tumor formation, progression and metastasis. In bone and tooth formation osteopontin is known to be a negative regulator of parathyroid hormone-related protein receptor, which induces osteogenesis. Without appropriate levels of osteopontin, bone growth continues unregulated, and leads to specific bone cancers. In short, osteopontin is a strong marker for bone growth, inflammation and certain cancers.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Osteopontin/OPN Antibody (NB110-89062). (Showing 1 - 1 of 1 FAQs).
I am very interested in your anti-human osteopontin (SPP1) (cat #NB110-89062). I work with bovine and would like to use the antibody in the blot. According to your description, the immunogen is a synthetic peptide which binds to the C-terminus of the human. The sequences of the C-termini are very similar in humans and cattle. Could you kindly tell me what amino acids against the C-terminus of the human antibody is directed exactly?
The immunogen sequence for NB110-89062 lies within amino acids 284-314 of isoform A, UniProt P10451. I have carried out a UniProt Blast analysis of the exact immunogen sequence versus Bos taurus (bovine) proteins for you, and the result was a 95% match for both osteopontin and osteopontin-K. This is a high percentage strongly suggesting that the antibody will be suitable for bovine, although we cannot guarantee it as this has not been tested in the lab. Because you would be using NB110-89062 with a species not stated on the web page / datasheet for this product, you would not be covered by the Novus Guarantee but you would be eligible for our special Innovator's Reward program, where as a thank you for sharing your results with us (whether the antibody proved suitable or not) by completing a review, we would give you a voucher code for the price of the product. Please contact us at firstname.lastname@example.org for more information.
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