Osteopontin/OPN Antibody (1B20) - BSA Free

Images

 
Immunohistochemistry: Osteopontin/OPN Antibody (1B20) [NB110-89062] - Osteopontin in (A) Group I, (B) Group II (C) Group III at 6 weeks. The pictures are arranged by staining technique (columns) and the investigated ...read more
Western Blot: Osteopontin/OPN Antibody (1B20) [NB110-89062] - Analysis of Osteopontin expression in U2OS whole cell lysate.
Immunocytochemistry/ Immunofluorescence: Osteopontin/OPN Antibody (1B20) [NB110-89062] - Osteopontin antibody was tested at 1:50 in U2OS cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and ...read more
Immunohistochemistry: Osteopontin/OPN Antibody (1B20) [NB110-89062] - Immunohistological results at 12 weeks for (A) Group I, (B) Group II (C) Group III.The pictures are arranged by staining technique (columns) and by ...read more
Immunohistochemistry: Osteopontin/OPN Antibody (1B20) [NB110-89062] - Analysis of Osteopontin on human lung adenocarcinoma.
Simple Western: Osteopontin/OPN Antibody (1B20) [NB110-89062] - Simple Western lane view shows a specific band for Osteopontin in 0.5 mg/ml of Human Breast Cancer lysate. This experiment was performed under reducing ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, RbSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
1B20
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Osteopontin/OPN Antibody (1B20) - BSA Free Summary

Immunogen
Synthetic peptide corresponding to the C-terminus of human Osteopontin [Swiss-Prot# P10451].
Localization
Secreted.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SPP1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
  • Simple Western 1:20
  • Western Blot 1:1000
Reviewed Applications
Read 2 Reviews rated 5
using
NB110-89062 in the following applications:

Publications
Read Publications using
NB110-89062 in the following applications:

Reactivity Notes

Human, Rat and Rabbit. Rabbit reactivity reported in scientific literature (PMID:33001866). Use in Mouse reported in scientific literature (PMID:33580126).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS pH 7.4
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Osteopontin/OPN Antibody (1B20) - BSA Free

  • BNSP
  • Bone sialoprotein 1
  • Eta-1
  • MGC110940
  • Nephropontin
  • OPN
  • Osteopontin
  • secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
  • secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
  • Spp1
  • SPP-1
  • SPP1/CALPHA1 fusion
  • Urinary stone protein
  • uropontin

Background

The matricellular protein osteopontin binds to cell surface receptors and is secreted into many body fluids including milk, blood, and urine, depending on the organ of origin. This makes osteopontin an ideal candidate for being a biomarker as the secreted form is easily obtained in throwaway fluids, and mimics the cellular environment from which it is released. Osteopontin is important in immune responses and inflammation as well as bone generation and remodeling. In autistic children, serum levels of osteopontin are correlated to the severity of disease, probably due to a brain inflammation pattern in these children. In aortic valve sclerosis and stenosis, increased levels of secreted osteopontin are also noted. Osteopontin has also been suggested as a cancer biomarker, since it is associated with tumor formation, progression and metastasis. In bone and tooth formation osteopontin is known to be a negative regulator of parathyroid hormone-related protein receptor, which induces osteogenesis. Without appropriate levels of osteopontin, bone growth continues unregulated, and leads to specific bone cancers. In short, osteopontin is a strong marker for bone growth, inflammation and certain cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for Osteopontin/OPN Antibody (NB110-89062)(28)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Rabbit.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(5)
IHC
(6)
IHC-Fr
(2)
IHC-P
(7)
WB
(5)
All Applications
Filter By Species
Human
(12)
Mouse
(2)
Rat
(4)
Rabbit
(7)
All Species
Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using NB110-89062 Applications Species
Fan C, Li Z, Ji Q et al. Carboxymethyl chitin or chitosan for osteoinduction effect on the human periodontal ligament stem cells Dental materials journal Mar 4 2022 [PMID: 35249903] (ICC/IF, Human) ICC/IF Human
Oliveros Anerillas L, Kingham PJ, Lammi MJ et al. Three-Dimensional Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells Promotes Matrix Metallopeptidase 13 (MMP13) Expression in Type I Collagen Hydrogels International journal of molecular sciences Dec 18 2021 [PMID: 34948393] (IHC, Human) IHC Human
Acharya TK, Kumar S, Tiwari N et al. TRPM8 channel inhibitor-encapsulated hydrogel as a tunable surface for bone tissue engineering Scientific reports Feb 12 2021 [PMID: 33580126] (ICC/IF, Mouse) ICC/IF Mouse
Padalhin A, Ventura R, Kim B, et al. Boosting osteogenic potential and bone regeneration by co-cultured cell derived extracellular matrix incorporated porous electrospun scaffold Journal of biomaterials science. Polymer edition Jan 12 2021 [PMID: 33375905]
Kerns ML, Miller RJ, Mazhar M et al. Experimental and clinical evaluation of BMP2-CPC graft versus deproteinized bovine bone graft for guided bone regeneration: A pilot study JCI Insight Oct 7 2020 [PMID: 33001866] (IHC, Rabbit) IHC Rabbit
Yu J, Berga SL, Zou W et al. Human Endometrial Stromal Cell Differentiation is Stimulated by PPAR beta/delta Activation: New Targets for Infertility? J. Clin. Endocrinol. Metab. Sep 1 2020 [PMID: 32594141]
Scimeca M, Anemona L, Granaglia A et al. Plaque calcification is driven by different mechanisms of mineralization associated with specific cardiovascular risk factors Nutr Metab Cardiovasc Dis 2019 Aug 23 [PMID: 31653516] (IHC, Human) IHC Human
AlyesSary AS, Yap AU, Othman Sa et al. Is there an optimal initial amount of activation for midpalatal suture expansion. : A histomorphometric and immunohistochemical study in a rabbit model. J Orofac Orthop Apr 11 2018 [PMID: 29644389] (Rabbit) Rabbit
Jayash SN, Hashim NM, Misran M, Baharuddin NA. Local application of osteoprotegerin-chitosan gel in critical-sized defects in a rabbit model PeerJ 2017 Jul 04 [PMID: 28674665] (IHC-P, Rabbit) IHC-P Rabbit
Wada S, Kanzaki H, Narimiya T, Nakamura Y. Novel device for application of continuous mechanical tensile strain to mammalian cells. Biol Open. Mar 16 2017 [PMID: 28302667] (WB, Human) WB Human
Show All 28 Publications.

Reviews for Osteopontin/OPN Antibody (NB110-89062) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB110-89062:
Filter by Applications
Immunofluorescence - fixed-frozen
(1)
ELISA
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Other Osteopontin/OPN NB110-89062
Enlarge
5
reviewed by:
Christian Schuerch
Immunofluorescence - fixed-frozen Mouse 02/29/2020
View

Summary

ApplicationOther
Sample Testedbone marrow
SpeciesMouse
LotI don't have it at hand

Comments

CommentsMouse bones were fixed in PFA 4% for 4h on ice, decalcified in EDTA for 24h at 4°C, cryoprotected in 30% sucrose for 24 at 4°C, and embedded in OCT
  5
reviewed by:
Verified Customer
ELISA Human 08/11/2017
View

Summary

ApplicationELISA
Sample TestedHuman breast cancer plasma,Human breast carcinoma
SpeciesHuman
LotB-1, B-2, B-3, B-4, B-5, B-6

Comments

CommentsIt will be nice if using PBS buffer for product. Removing Gly-Tris buffer is a pain.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Osteopontin/OPN Antibody (NB110-89062). (Showing 1 - 1 of 1 FAQs).

  1. I am very interested in your anti-human osteopontin (SPP1) (cat #NB110-89062). I work with bovine and would like to use the antibody in the blot. According to your description, the immunogen is a synthetic peptide which binds to the C-terminus of the human. The sequences of the C-termini are very similar in humans and cattle. Could you kindly tell me what amino acids against the C-terminus of the human antibody is directed exactly?
    • The immunogen sequence for NB110-89062 lies within amino acids 284-314 of isoform A, UniProt P10451. I have carried out a UniProt Blast analysis of the exact immunogen sequence versus Bos taurus (bovine) proteins for you, and the result was a 95% match for both osteopontin and osteopontin-K. This is a high percentage strongly suggesting that the antibody will be suitable for bovine, although we cannot guarantee it as this has not been tested in the lab. Because you would be using NB110-89062 with a species not stated on the web page / datasheet for this product, you would not be covered by the Novus Guarantee but you would be eligible for our special Innovator's Reward program, where as a thank you for sharing your results with us (whether the antibody proved suitable or not) by completing a review, we would give you a voucher code for the price of the product. Please contact us at innovators@novusbio.com for more information.

Secondary Antibodies

 

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Recent Reviews

5
5
2
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2
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1
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Christian Schuerch
02/29/2020
Application: Immunofluorescence - fixed-frozen
Species: Mouse

 
Verified Customer
08/11/2017
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol SPP1
Entrez
Uniprot