Osteopontin/OPN Antibody (1B20) [FITC]


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Product Details

Reactivity Hu, Rt, RbSpecies Glossary
Applications WB, IHC, IHC-P, IP
Please see the vial label for concentration. If unlisted please contact technical services.

Osteopontin/OPN Antibody (1B20) [FITC] Summary

A synthetic peptide corresponding to the C-terminus of human Osteopontin [Swiss-Prot# P10451].
Protein A purified
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Packaging, Storage & Formulations

Store at 4C in the dark.
FITC (A=495, E=519)
0.05% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
Protein A purified


Application Notes
This Osteopontin (1B20) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In Western blot multiple bands can be seen due to glycosylation and phosphorylation of the protein. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24269728)

Reactivity Notes

Human, Rat and Rabbit.

Alternate Names for Osteopontin/OPN Antibody (1B20) [FITC]

  • BNSP
  • Bone sialoprotein 1
  • Eta-1
  • MGC110940
  • Nephropontin
  • OPN
  • osteopontin
  • secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
  • secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
  • Spp1
  • SPP-1
  • SPP1/CALPHA1 fusion
  • Urinary stone protein
  • uropontin


The matricellular protein osteopontin binds to cell surface receptors and is secreted into many body fluids including milk, blood and urine, depending on the organ of origin. This makes osteopontin an ideal candidate for being a biomarker as the secreted form is easily obtained in throwaway fluids, and mimics the cellular environment from which it is released. Osteopontin is important in immune responses and inflammation as well as bone generation and remodeling. In autistic children, serum levels of osteopontin are correlated to the severity of disease, probably due to a brain inflammation pattern in these children. In aortic valve sclerosis and stenosis, increased levels of secreted osteopontin are also noted. Osteopontin has also been suggested as a cancer biomarker, since it is associated with tumor formation, progression and metastasis. In bone and tooth formation osteopontin is known to be a negative regulator of parathyroid hormone-related protein receptor, which induces osteogenesis. Without appropriate levels of osteopontin, bone growth continues unregulated, and leads to specific bone cancers. In short, osteopontin is a strong marker for bone growth, inflammation and certain cancers.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Osteopontin/OPN Antibody (NB110-89062F) (0)

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Secondary Antibodies


Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB110-89062APC
Biotin Labeled NB110-89062B
DyLight 350 Labeled NB110-89062UV
DyLight 405 Labeled NB110-89062V
DyLight 405LS Labeled NB110-89062V3
DyLight 488 Labeled NB110-89062G
DyLight 550 Labeled NB110-89062R
DyLight 650 Labeled NB110-89062C
DyLight 680 Labeled NB110-89062FR
DyLight 755 Labeled NB110-89062IR
HRP Labeled NB110-89062H
PE Labeled NB110-89062PE
PerCP Labeled NB110-89062PCP

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Contact Information

Product PDFs


Gene Symbol SPP1

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