Alkaline Phosphatase/ALPP Antibody (8B6)


Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Representative montage images are shown for control dissociated DRG culture. (B) Dissociated DRGs cultures exposed to more
Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - analysis of ALPP in MDA-MB-231 cells using an anti-ALPP antibody (blue - cell membrane, green - ALPP). Image from verified more
Western Blot: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Analysis of Alkaline Phosphatase (Placental) expression in JAR whole cell lysate.
Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Alkaline Phosphatase, Placental Antibody (8B6) [NB110-3638] - IHC staining of Alkaline Phosphatase (Placental) in human placenta more
Flow Cytometry: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Detection of Alkaline Phosphatase/ALPP in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- Alkaline more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, RI
1.0 mg/ml

Order Details

Alkaline Phosphatase/ALPP Antibody (8B6) Summary

Hep-2 cells with boosted surface expression of Alkaline Phosphatase, Placental. [UniProt# P05187].
Cell membrane; Lipid-anchor > GPI-anchor
Alkaline Phosphatase, Placental - both Regan and Nagao isoenzymes. No cross reactivity with other isoenzymes of Alkaline Phosphatase.
IgG2a Kappa
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Radioimmunodiffusion
  • Western Blot 1:1000
Application Notes
This Alkaline Phosphatase, Placental Antibody (8B6) is useful for IHC-P sections and frozen sections, ICC/IF, ELISA, WB and Radioimmunodiffusion. In WB a band can be seen at ~70 kDa. For IHC, Proteolytic Induced Epitope Retrieval (PIER) is required. Use in Flow Cytometry reported in various pieces of scientific literature.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
NB110-3638 in the following applications:

Read Publications using
NB110-3638 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28197547). Rat reactivity reported in scientific literature (PMID: 30599898).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein A purified

Alternate Names for Alkaline Phosphatase/ALPP Antibody (8B6)

  • Alkaline phosphatase Regan isozyme
  • Alkaline Phosphatase
  • alkaline phosphatase, placental (Regan isozyme)
  • alkaline phosphatase, placental type
  • alkaline phosphatase, placental
  • alkaline phosphomonoesterase
  • ALP
  • ALPP
  • EC
  • FLJ61142
  • glycerophosphatase
  • PALP
  • Placental alkaline phosphatase 1
  • PLAP
  • PLAP-1
  • Regan isozyme
  • SEAP


Alkaline Phosphatase, Placental, also known as PLAP and ALPP is an alkaline phosphatase located on the second chromosome. Alkaline phosphatases are phosphodiesterases. The placental-specific isozyme of Alkaline Phosphatase is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. The detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer (PMID: 21809268).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Alkaline Phosphatase/ALPP Antibody (NB110-3638)(12)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: FLOW, ICC/IF, IHC, IHC-Fr, WB.

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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB110-3638 Applications Species
Ontawong A, Duangjai A, Muanprasat C et al. Lipid-lowering effects of Coffea arabica pulp aqueous extract in Caco-2 cells and hypercholesterolemic rats. Phytomedicine Jun 1 2018 [PMID: 30599898] (WB, Human, Rat) WB Human, Rat
Goulao M, Ghosh B, Urban MW et al. Astrocyte progenitor transplantation promotes regeneration of bulbospinal respiratory axons, recovery of diaphragm function, and a reduced macrophage response following cervical spinal cord injury Glia Dec 11 2018 [PMID: 30548313] (IHC, Human) IHC Human
Kim J, Singh A, DelPoeta M et al. The effect of sterol structure upon clathrin-mediated and clathrin-independent endocytosis. J Cell Sci. [PMID: 28655854] (Human) Human
Merianda TT, Jin Y, Kalinski AL et al. Neural Progenitor Cells Promote Axonal Growth and Alter Axonal mRNA Localization in Adult Neurons. eNeuro. 2017 Feb 15 [PMID: 28197547] (ICC/IF, Mouse) ICC/IF Mouse
Forbes K, Desforges M, Garside R et al. Methods for siRNA-mediated reduction of mRNA and protein expression in human placental explants, isolated primary cells and cell lines. Placenta. 2009 Feb [PMID: 19012963] (ICC/IF, Human) ICC/IF Human
Johnstone ED, Chan G, Sibley CP et al. Sphingosine-1-phosphate inhibition of placental trophoblast differentiation through a G(i)-coupled receptor response. J Lipid Res. 2005 Sep [PMID: 15995175] (ICC/IF, Human) ICC/IF Human
Leitner K, Szlauer R, Ellinger I et al. Placental alkaline phosphatase expression at the apical and basal plasma membrane in term villous trophoblasts. J Histochem Cytochem. 2001 Sep [PMID: 11511684] (IHC-Fr, ICC/IF, Human) IHC-Fr, ICC/IF Human
Kiem HP, Andrews RG, Morris J et al. Improved gene transfer into baboon marrow repopulating cells using recombinant human fibronectin fragment CH-296 in combination with interleukin-6, stem cell factor, FLT-3 ligand, and megakaryocyte growth and development factor. Blood. 1998 Sep 15 [PMID: 9731044] (FLOW) FLOW
Feehan C, Darlak K, Kahn J, et al. Shedding of the lymphocyte L-selectin adhesion molecule is inhibited by a hydroxamic acid-based protease inhibitor. Identification with an L-selectin-alkaline phosphatase reporter. J Biol Chem. 1996 Mar 22 [PMID: 8636132] (FLOW, Human) FLOW Human
Roberts SB, Ripellino JA, Ingalls KM et al. Non-amyloidogenic cleavage of the beta-amyloid precursor protein by an integral membrane metalloendopeptidase. J Biol Chem. 1994 Jan 28 [PMID: 8300647] (WB, Human) WB Human
Show All 12 Publications.

Review for Alkaline Phosphatase/ALPP Antibody (NB110-3638) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB110-3638:
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Images Ratings Applications Species Date Details
Immunofluorescence Alkaline Phosphatase/ALPP NB110-3638
reviewed by:
Verified Customer
IF Human 11/24/2014


Sample TestedMDA-MB-231 cell


Blocking DetailsNo blocking.

Primary Anitbody

Dilution RatioDilution of the antibody at 20µg/ml in HBSS with 3% BSA, incubation at 4 degrees for 1 hour.

Secondary Antibody

Secondary DescriptionAF488 goat anti-mouse IgG
Secondary Manufacturer Cat#Molecular Probes, A11001
Secondary Concentration40µg/ml in HBSS with 3% BSA


Detection NotesUsing confocal-microscope
Fixation DetailsCoverslip-attached living cells in PBS at 4 degrees.
Wash DescriptionUsing PBS, no incubation time, 2ml/35mm dish, repeat three times.

Product General Protocols

View specific protocols for Alkaline Phosphatase/ALPP Antibody (NB110-3638): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Alkaline Phosphatase/ALPP Antibody (NB110-3638). (Showing 1 - 2 of 2 FAQs).

  1. I am looking to use a placental alkaline phosphatase antibody for sorting syncytiotrophoblast material from female tissue. It looks like most people who use these antibodies use in-house versions and references for commercially available antibodies I've found are from the '80's. I noticed you have several different monoclonal antibodies and was hoping you could give me a little more information on them and potential applications they've been tested for. Also, do you have a biotinylated or fluorophore-conjugated version?
    • None of our placental alkaline phosphatase antibodies are biotinylated or fluorchrome conjugated but we offer a number of kits for this application and our lab can do a custom conjugation as well, for an additional fee. NB600-750 is useful in FACS with human cells, so I would recommend this antibody for your experiment. We back the use of this antibody as stated on the datasheet with the 100% Novus Guarantee. If you have questions about another antibody, I will be happy to answer them.
  2. Our customer asked us to submit the inquiry to confirm if it is true that catalog # NB110-3638 has not been tested for WB. The customer seems to believe NB110-3638 shall be able to be used for WB.
    • NB110-3638 has not been tested in Western blot and we cannot guarantee that application. Please advise your customer that the applications listed on the datasheet are the ones we have tested and will guarantee.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-3638AF350
Alexa Fluor 405 NB110-3638AF405
Alexa Fluor 488 NB110-3638AF488
Alexa Fluor 532 NB110-3638AF532
Alexa Fluor 594 NB110-3638AF594
Alexa Fluor 647 NB110-3638AF647
Alexa Fluor 700 NB110-3638AF700
Alexa Fluor 750 NB110-3638AF750
Allophycocyanin NB110-3638APC
Allophycocyanin/Cy7 NB110-3638APCCY7
Biotin NB110-3638B
DyLight 350 NB110-3638UV
DyLight 405 NB110-3638V
DyLight 488 NB110-3638G
DyLight 550 NB110-3638R
DyLight 594 NB110-3638DL594
DyLight 650 NB110-3638C
DyLight 680 NB110-3638FR
DyLight 755 NB110-3638IR
FITC NB110-3638F
HRP NB110-3638H
Janelia Fluor 549 NB110-3638JF549
Janelia Fluor 646 NB110-3638JF646
PE NB110-3638PE
PE/Atto594 NB110-3638PEATT594
PE/Cy5.5 NB110-3638PECY55
PE/Cy7 NB110-3638PECY7
PerCP NB110-3638PCP

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Recent Reviews


Verified Customer
Application: IF
Species: Human


Gene Symbol ALPP