Western Blot: Alkaline Phosphatase, Placental Antibody (8B6) [NB110-3638] - Analysis of Alkaline Phosphatase (Placental) expression in JAR whole cell lysate.
Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Representative montage images are shown for control dissociated DRG culture. (B) Dissociated DRGs cultures exposed to ...read more
Immunohistochemistry-Paraffin: Alkaline Phosphatase, Placental Antibody (8B6) [NB110-3638] - IHC staining of Alkaline Phosphatase (Placental) in human placenta using DAB with hematoxylin counterstain. Proteolytic ...read more
Flow Cytometry: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Detection of Alkaline Phosphatase/ALPP in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- Alkaline ...read more
Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - analysis of ALPP in MDA-MB-231 cells using an anti-ALPP antibody (blue - cell membrane, green - ALPP). Image from verified ...read more
This Alkaline Phosphatase, Placental Antibody (8B6) is useful for IHC-P sections and frozen sections, ICC/IF, ELISA, WB and Radioimmunodiffusion. In WB a band can be seen at ~70 kDa. For IHC, Proteolytic Induced Epitope Retrieval (PIER) is required. Use in Flow Cytometry reported in various pieces of scientific literature.
70 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Mouse reactivity reported in scientific literature (PMID: 28197547). Rat reactivity reported in scientific literature (PMID: 30599898).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
Protein A purified
Alternate Names for Alkaline Phosphatase/ALPP Antibody (8B6)
Alkaline phosphatase Regan isozyme
alkaline phosphatase, placental (Regan isozyme)
alkaline phosphatase, placental type
alkaline phosphatase, placental
Placental alkaline phosphatase 1
Alkaline Phosphatase, Placental, also known as PLAP and ALPP is an alkaline phosphatase located on the second chromosome. Alkaline phosphatases are phosphodiesterases. The placental-specific isozyme of Alkaline Phosphatase is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. The detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer (PMID: 21809268).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Alkaline Phosphatase/ALPP Antibody (NB110-3638). (Showing 1 - 2 of 2 FAQs).
I am looking to use a placental alkaline phosphatase antibody for sorting syncytiotrophoblast material from female tissue. It looks like most people who use these antibodies use in-house versions and references for commercially available antibodies I've found are from the '80's. I noticed you have several different monoclonal antibodies and was hoping you could give me a little more information on them and potential applications they've been tested for. Also, do you have a biotinylated or fluorophore-conjugated version?
None of our placental alkaline phosphatase antibodies are biotinylated or fluorchrome conjugated but we offer a number of kits for this application and our lab can do a custom conjugation as well, for an additional fee. NB600-750 is useful in FACS with human cells, so I would recommend this antibody for your experiment. We back the use of this antibody as stated on the datasheet with the 100% Novus Guarantee. If you have questions about another antibody, I will be happy to answer them.
Our customer asked us to submit the inquiry to confirm if it is true that catalog # NB110-3638 has not been tested for WB. The customer seems to believe NB110-3638 shall be able to be used for WB.
NB110-3638 has not been tested in Western blot and we cannot guarantee that application. Please advise your customer that the applications listed on the datasheet are the ones we have tested and will guarantee.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.