Alkaline Phosphatase/ALPP Antibody (8B6)

Images

 
Western Blot: Alkaline Phosphatase, Placental Antibody (8B6) [NB110-3638] - Analysis of Alkaline Phosphatase (Placental) expression in JAR whole cell lysate.
Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - analysis of ALPP in MDA-MB-231 cells using an anti-ALPP antibody (blue - cell membrane, green - ALPP). Image from verified ...read more
Immunohistochemistry-Paraffin: Alkaline Phosphatase, Placental Antibody (8B6) [NB110-3638] - IHC staining of Alkaline Phosphatase (Placental) in human placenta using DAB with hematoxylin counterstain. Proteolytic ...read more
Flow Cytometry: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Detection of Alkaline Phosphatase/ALPP in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- Alkaline ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, RI
Clone
8B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Alkaline Phosphatase/ALPP Antibody (8B6) Summary

Immunogen
Hep-2 cells with boosted surface expression of Alkaline Phosphatase, Placental. [UniProt# P05187].
Localization
Cell membrane; Lipid-anchor > GPI-anchor
Specificity
Alkaline Phosphatase, Placental - both Regan and Nagao isoenzymes. No cross reactivity with other isoenzymes of Alkaline Phosphatase.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ALPP
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Radioimmunodiffusion
Application Notes
This Alkaline Phosphatase, Placental Antibody (8B6) is useful for Immunohistochemistry on paraffin-embedded sections and frozen sections, Immunocytochemistry/Immunofluorescence, ELISA, Western blot and Radioimmunodiffusion. In WB a band can be seen at ~70 kDa. For IHC, Proteolytic Induced Epitope Retrieval (PIER) is required. Use in Flow Cytometry reported in various pieces of scientific literature The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB110-3638 in the following applications:

Publications
Read Publications using
NB110-3638 in the following applications:

Reactivity Notes

Human. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Alkaline Phosphatase/ALPP Antibody (8B6)

  • Alkaline phosphatase Regan isozyme
  • Alkaline Phosphatase
  • alkaline phosphatase, placental (Regan isozyme)
  • alkaline phosphatase, placental type
  • alkaline phosphatase, placental
  • alkaline phosphomonoesterase
  • ALP
  • ALPP
  • EC 3.1.3.1
  • FLJ61142
  • glycerophosphatase
  • PALP
  • Placental alkaline phosphatase 1
  • PLAP
  • PLAP-1
  • Regan isozyme
  • SEAP

Background

Alkaline Phosphatase, Placental, also known as PLAP and ALPP is an alkaline phosphatase located on the second chromosome. Alkaline phosphatases are phosphodiesterases. The placental-specific isozyme of Alkaline Phosphatase is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. The detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer (PMID: 21809268).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Alkaline Phosphatase/ALPP Antibody (NB110-3638)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: FLOW, ICC/IF, IHC-Fr, WB.


Filter By Application
FLOW
(2)
ICC/IF
(4)
IHC-Fr
(2)
WB
(1)
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Human
(6)
Mouse
(1)
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Showing Publications 1 - 10 of 10.
Publications using NB110-3638 Applications Species
Kim J, Singh A, DelPoeta M et al. The effect of sterol structure upon clathrin-mediated and clathrin-independent endocytosis. J. Cell. Sci. 2017 Jun 27 [PMID: 28655854]
Kim J, Singh A, DelPoeta M et al. The effect of sterol structure upon clathrin-mediated and clathrin-independent endocytosis. Journal of Cell Science 2017 Jun 27 [PMID: 28655854]
Merianda TT, Jin Y, Kalinski AL et al. Neural Progenitor Cells Promote Axonal Growth and Alter Axonal mRNA Localization in Adult Neurons. eNeuro. 2017 Feb 15 [PMID: 28197547] (ICC/IF, Mouse) ICC/IF Mouse
Forbes K, Desforges M, Garside R et al. Methods for siRNA-mediated reduction of mRNA and protein expression in human placental explants, isolated primary cells and cell lines. Placenta. 2009 Feb [PMID: 19012963] (ICC/IF, Human) ICC/IF Human
Johnstone ED, Chan G, Sibley CP et al. Sphingosine-1-phosphate inhibition of placental trophoblast differentiation through a G(i)-coupled receptor response. J Lipid Res. 2005 Sep [PMID: 15995175] (ICC/IF, Human) ICC/IF Human
Leitner K, Szlauer R, Ellinger I et al. Placental alkaline phosphatase expression at the apical and basal plasma membrane in term villous trophoblasts. J Histochem Cytochem. 2001 Sep [PMID: 11511684] (IHC-Fr, ICC/IF, Human) IHC-Fr, ICC/IF Human
Kiem HP, Andrews RG, Morris J et al. Improved gene transfer into baboon marrow repopulating cells using recombinant human fibronectin fragment CH-296 in combination with interleukin-6, stem cell factor, FLT-3 ligand, and megakaryocyte growth and development factor. Blood. 1998 Sep 15 [PMID: 9731044] (FLOW) FLOW
Feehan C, Darlak K, Kahn J, et al. Shedding of the lymphocyte L-selectin adhesion molecule is inhibited by a hydroxamic acid-based protease inhibitor. Identification with an L-selectin-alkaline phosphatase reporter. J Biol Chem. 1996 Mar 22 [PMID: 8636132] (FLOW, Human) FLOW Human
Roberts SB, Ripellino JA, Ingalls KM et al. Non-amyloidogenic cleavage of the beta-amyloid precursor protein by an integral membrane metalloendopeptidase. J Biol Chem. 1994 Jan 28 [PMID: 8300647] (WB, Human) WB Human
Kesson AM, Fear WR, Williams L et al. HIV infection of placental macrophages: their potential role in vertical transmission. J Leukoc Biol. 1994 Sep [PMID: 8083596] (IHC-Fr, Human) IHC-Fr Human

Review for Alkaline Phosphatase/ALPP Antibody (NB110-3638) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IF.

Reviews using NB110-3638:
Filter by Applications
IF
(1)
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Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Alkaline Phosphatase/ALPP NB110-3638
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reviewed by:
Anonymous
IF Human 11/24/2014
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Summary

ApplicationImmunofluorescence
Sample TestedMDA-MB-231 cell
SpeciesHuman
LotA-1

Blocking

Blocking DetailsNo blocking.

Primary Anitbody

Dilution RatioDilution of the antibody at 20µg/ml in HBSS with 3% BSA, incubation at 4 degrees for 1 hour.

Secondary Antibody

Secondary DescriptionAF488 goat anti-mouse IgG
Secondary Manufacturer Cat#Molecular Probes, A11001
Secondary Concentration40µg/ml in HBSS with 3% BSA

Details

Detection NotesUsing confocal-microscope
Fixation DetailsCoverslip-attached living cells in PBS at 4 degrees.
Wash DescriptionUsing PBS, no incubation time, 2ml/35mm dish, repeat three times.

Product General Protocols

View specific protocols for Alkaline Phosphatase/ALPP Antibody (NB110-3638): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Alkaline Phosphatase/ALPP Antibody (NB110-3638). (Showing 1 - 2 of 2 FAQs).

  1. I am looking to use a placental alkaline phosphatase antibody for sorting syncytiotrophoblast material from female tissue. It looks like most people who use these antibodies use in-house versions and references for commercially available antibodies I've found are from the '80's. I noticed you have several different monoclonal antibodies and was hoping you could give me a little more information on them and potential applications they've been tested for. Also, do you have a biotinylated or fluorophore-conjugated version?
    • None of our placental alkaline phosphatase antibodies are biotinylated or fluorchrome conjugated but we offer a number of kits for this application and our lab can do a custom conjugation as well, for an additional fee. NB600-750 is useful in FACS with human cells, so I would recommend this antibody for your experiment. We back the use of this antibody as stated on the datasheet with the 100% Novus Guarantee. If you have questions about another antibody, I will be happy to answer them.
  2. Our customer asked us to submit the inquiry to confirm if it is true that catalog # NB110-3638 has not been tested for WB. The customer seems to believe NB110-3638 shall be able to be used for WB.
    • NB110-3638 has not been tested in Western blot and we cannot guarantee that application. Please advise your customer that the applications listed on the datasheet are the ones we have tested and will guarantee.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 405 Labeled NB110-3638V
FITC Labeled NB110-3638F

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Recent Reviews

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1
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1
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Anonymous
11/24/2014
Application: IF
Species: Human

Bioinformatics

Gene Symbol ALPP
Entrez
Uniprot