Alkaline Phosphatase/ALPP Antibody (8B6)


Western Blot: Alkaline Phosphatase, Placental Antibody (8B6) [NB110-3638] - Analysis of Alkaline Phosphatase (Placental) expression in JAR whole cell lysate.
Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - analysis of ALPP in MDA-MB-231 cells using an anti-ALPP antibody (blue - cell membrane, green - ALPP). Image from verified more
Immunohistochemistry-Paraffin: Alkaline Phosphatase, Placental Antibody (8B6) [NB110-3638] - IHC staining of Alkaline Phosphatase (Placental) in human placenta using DAB with hematoxylin counterstain. Proteolytic more

Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, RI
1.0 mg/ml

Alkaline Phosphatase/ALPP Antibody (8B6) Summary

Hep-2 cells with boosted surface expression of Alkaline Phosphatase, Placental. [UniProt# P05187].
Cell membrane; Lipid-anchor > GPI-anchor
Alkaline Phosphatase, Placental - both Regan and Nagao isoenzymes. No cross reactivity with other isoenzymes of Alkaline Phosphatase.
IgG2a Kappa
Protein A purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
1.0 mg/ml
Protein A purified


  • Western Blot 1:1000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Radioimmunodiffusion
Application Notes
This Alkaline Phosphatase, Placental Antibody (8B6) is useful for Immunohistochemistry on paraffin-embedded sections and frozen sections, Immunocytochemistry/Immunofluorescence, ELISA, Western blot and Radioimmunodiffusion. In WB a band can be seen at ~70 kDa. For IHC, Proteolytic Induced Epitope Retrieval (PIER) is required. Use in Flow Cytometry reported in various pieces of scientific literature

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
NB110-3638 in the following applications:

Read Publications using
NB110-3638 in the following applications:

Reactivity Notes

Human. Not yet tested in other species.

Alternate Names for Alkaline Phosphatase/ALPP Antibody (8B6)

  • Alkaline phosphatase Regan isozyme
  • Alkaline Phosphatase
  • alkaline phosphatase, placental (Regan isozyme)
  • alkaline phosphatase, placental type
  • alkaline phosphatase, placental
  • alkaline phosphomonoesterase
  • ALP
  • EC
  • FLJ61142
  • glycerophosphatase
  • PALP
  • Placental alkaline phosphatase 1
  • PLAP
  • PLAP-1
  • Regan isozyme
  • SEAP


Alkaline Phosphatase, Placental, also known as PLAP and ALPP is an alkaline phosphatase located on the second chromosome. Alkaline phosphatases are phosphodiesterases. The placental-specific isozyme of Alkaline Phosphatase is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. The detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer (PMID: 21809268).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Alkaline Phosphatase/ALPP Antibody (NB110-3638)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: FLOW, ICC/IF, IHC-Fr, WB.

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Showing Publications 1 - 7 of 7.
Publications using NB110-3638 Applications Species
Forbes K, Desforges M, Garside R et al. Methods for siRNA-mediated reduction of mRNA and protein expression in human placental explants, isolated primary cells and cell lines. Placenta. 2009 Feb [PMID:19012963] (ICC/IF, Human) ICC/IF Human
Johnstone ED, Chan G, Sibley CP et al. Sphingosine-1-phosphate inhibition of placental trophoblast differentiation through a G(i)-coupled receptor response. J Lipid Res. 2005 Sep [PMID:15995175] (ICC/IF, Human) ICC/IF Human
Leitner K, Szlauer R, Ellinger I et al. Placental alkaline phosphatase expression at the apical and basal plasma membrane in term villous trophoblasts. J Histochem Cytochem. 2001 Sep [PMID:11511684] (IHC-Fr, ICC/IF, Human) IHC-Fr, ICC/IF Human
Kiem HP, Andrews RG, Morris J et al. Improved gene transfer into baboon marrow repopulating cells using recombinant human fibronectin fragment CH-296 in combination with interleukin-6, stem cell factor, FLT-3 ligand, and megakaryocyte growth and development factor. Blood. 1998 Sep 15 [PMID:9731044] (FLOW) FLOW
Feehan C, Darlak K, Kahn J, et al. Shedding of the lymphocyte L-selectin adhesion molecule is inhibited by a hydroxamic acid-based protease inhibitor. Identification with an L-selectin-alkaline phosphatase reporter. J Biol Chem. 1996 Mar 22 [PMID:8636132] (FLOW, Human) FLOW Human
Roberts SB, Ripellino JA, Ingalls KM et al. Non-amyloidogenic cleavage of the beta-amyloid precursor protein by an integral membrane metalloendopeptidase. J Biol Chem. 1994 Jan 28 [PMID:8300647] (WB, Human) WB Human
Kesson AM, Fear WR, Williams L et al. HIV infection of placental macrophages: their potential role in vertical transmission. J Leukoc Biol. 1994 Sep [PMID:8083596] (IHC-Fr, Human) IHC-Fr Human

Reviews for Alkaline Phosphatase/ALPP Antibody (NB110-3638) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.

Reviews using NB110-3638:
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Images Ratings Applications Species Date Details
 Alkaline Phosphatase/ALPP NB110-3638
reviewed by:
Human 11/24/2014


Sample TestedMDA-MB-231 cell


Blocking DetailsNo blocking.

Primary Anitbody

Dilution RatioDilution of the antibody at 20µg/ml in HBSS with 3% BSA, incubation at 4 degrees for 1 hour.

Secondary Antibody

Secondary DescriptionAF488 goat anti-mouse IgG
Secondary Manufacturer Cat#Molecular Probes, A11001
Secondary Concentration40µg/ml in HBSS with 3% BSA


Detection NotesUsing confocal-microscope
Fixation DetailsCoverslip-attached living cells in PBS at 4 degrees.
Wash DescriptionUsing PBS, no incubation time, 2ml/35mm dish, repeat three times.

Product General Protocols

View specific protocols for Alkaline Phosphatase/ALPP Antibody (NB110-3638): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Alkaline Phosphatase/ALPP Antibody (NB110-3638). (Showing 1 - 2 of 2 FAQs).

  1. I am looking to use a placental alkaline phosphatase antibody for sorting syncytiotrophoblast material from female tissue. It looks like most people who use these antibodies use in-house versions and references for commercially available antibodies I've found are from the '80's. I noticed you have several different monoclonal antibodies and was hoping you could give me a little more information on them and potential applications they've been tested for. Also, do you have a biotinylated or fluorophore-conjugated version?
    • None of our placental alkaline phosphatase antibodies are biotinylated or fluorchrome conjugated but we offer a number of kits for this application and our lab can do a custom conjugation as well, for an additional fee. NB600-750 is useful in FACS with human cells, so I would recommend this antibody for your experiment. We back the use of this antibody as stated on the datasheet with the 100% Novus Guarantee. If you have questions about another antibody, I will be happy to answer them.
  2. Our customer asked us to submit the inquiry to confirm if it is true that catalog # NB110-3638 has not been tested for WB. The customer seems to believe NB110-3638 shall be able to be used for WB.
    • NB110-3638 has not been tested in Western blot and we cannot guarantee that application. Please advise your customer that the applications listed on the datasheet are the ones we have tested and will guarantee.

Secondary Antibodies


Isotype Controls

Other Available Formats

DyLight 405 Labeled NB110-3638V
FITC Labeled NB110-3638F

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Contact Information

Product PDFs


Gene Symbol ALPP

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