NRIP Antibody

Western Blot: NRIP Antibody [NB100-56434] - Analysis of NRIP in human muscle tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse muscle tissue lysate and 4) rat muscle tissue lysate using this antibody.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NRIP Antibody Summary

• Description: Novus Biologicals Rabbit NRIP Antibody (NB100-56434) is a polyclonal antibody validated for use in WB. Anti-NRIP Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 800-850 of human NRIP was used as the immunogen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DCAF6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-3 ug/ml
• Control:

  Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NRIP Antibody

• 1200006M05Rik
• Androgen receptor complex-associated protein
• ARCAP
• DDB1 and CUL4 associated factor 6
• DDB1- and CUL4-associated factor 6
• FLJ23798
• IQ motif and WD repeat-containing protein 1
• IQ motif and WD repeats 1
• IQWD1
• MSTP055
• NRIP
• Nuclear receptor interaction protein
• PC326

Background

WD-repeat proteins are a large family of eukaryotic proteins coordinating multi-protein complex assemblies. Their role has been implicated in multiple cellular processes including signal transduction, transcriptional regulation, cell cycle control and apoptosis. NRIP is a novel 860a.a nuclear protein consisting of seven conserved WD40 domains and one NLS motif. It binds to androgen and glucocorticoid receptors and up-regulates their transcriptional activity, thereby functioning as a nuclear receptor co-activator. Role of NRIP has been implicated in cell growth and also in cervical and prostate cancer, thus indicating a potential therapeutic intervention. Northern Blot analysis detects a high expression of NRIP in skeletal muscle and testis and low expression in heart, prostrate and adrenal gland.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NRIP Antibody (NB100-56434)(1)

Publication using NB100-56434

• Bruhn C, Bastianello G, Foiani M Cancer cell histone density links global histone acetylation, mitochondrial proteome and histone acetylase inhibitor sensitivity Communications biology 2022-08-27 [PMID: 36030322] (WB, Human)
  
  Details:
  Dilution used 1:1000

Bioinformatics

• Gene Symbol: DCAF6
• Entrez:
  • 55827(Human)
  • 74106(Mouse)
  • 289181(Rat)
• Uniprot:
  • Q58WW2(Human)