NGFR/TNFRSF16/p75NTR Antibody

Western Blot: NGFR/TNFRSF16/p75NTR Antibody [NBP2-19669] - Untreated (-) and treated (+) PC-3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p75 NGF Receptor / CD271 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: NGF R/TNFRSF16/p75NTR Antibody [NBP2-19669] - Culture rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p75 NGF Receptor protein stained by p75 NGF Receptor antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:250. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Frozen: NGF R/TNFRSF16/p75NTR Antibody [NBP2-19669] - Frozen sectioned E13.5 Rat brain. Green: p75 NGF Receptor protein stained by p75 NGF Receptor antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.

Western Blot: NGF R/TNFRSF16/p75NTR Antibody [NBP2-19669] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with p75 NGF Receptor antibody at a dilution of 1:2000.

Western Blot: NGF R/TNFRSF16/p75NTR Antibody [NBP2-19669] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p75 NGF Receptor / CD271 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: NGF R/TNFRSF16/p75NTR Antibody [NBP2-19669] - Rat brain. p75 NGF Receptor antibody diluted at 1:500.

Western Blot: NGFR/TNFRSF16/p75NTR Antibody [NBP2-19669] - Untreated (-) and treated (+) PC-3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NGFR/TNFRSF16/p75NTR antibody (NBP2-19669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, Simple Western, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NGFR/TNFRSF16/p75NTR Antibody Summary

• Description: Novus Biologicals Rabbit NGFR/TNFRSF16/p75NTR Antibody (NBP2-19669) is a polyclonal antibody validated for use in IHC, WB, Flow, ICC/IF and Simple Western. Anti-NGFR/TNFRSF16/p75NTR Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human NGFR/TNFRSF16/p75NTR. The exact sequence is proprietary.
• Localization: Membrane; Single-pass type I membrane protein
• Predicted Species: Bovine (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NGFR
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Simple Western 1:100
  • Western Blot 1:500-1:3000
• Application Notes: Flow-Assay dependent (Membrane permeabilization is required for this application).
  See Simple Western Antibody Database for Simple Western validation: Tested in Skin, separated by Size, antibody dilution of 1:100
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for NGFR/TNFRSF16/p75NTR Antibody

• CD271 antigen
• CD271
• Gp80-LNGFR
• member 16
• nerve growth factor receptor
• NGF R
• NGF receptor
• NGFR
• p75 ICD
• p75 NTR
• TNFRSF16
• TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16)
• tumor necrosis factor receptor superfamily member 16

Background

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for NGFR/TNFRSF16/p75NTR Antibody (NBP2-19669)(1)

Publication using NBP2-19669

Bioinformatics

• Gene Symbol: NGFR
• Uniprot:
  • P08138()