NG2/MCSP Antibody (LHM 2) - Azide and BSA Free

Western Blot: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] - Analysis of NG2 expression in HeLa whole cell lysate. Image from the standard format of this antibody.

Immunohistochemistry: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] - Staining of NG2 in human brain using DAB with hematoxylin counterstain. Image from the standard format of this antibody.

Flow Cytometry: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] - Antibody was tested at 1:400 in MCF7 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red). Image from the standard format of this antibody.

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] - A cell surface stain was performed on U87MG cells with NG2/MCSP antibody (LHM 2) NB100-2688AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] - A cell surface stain was performed on U87MG cells with NG2/MCSP antibody (LHM 2) NB100-2688PCP (blue) and a matched isotype control NBP2-27287PCP (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to PerCP complex.

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] - A surface stain was performed on A375 Cells with NG2/MCSP (LHM2) antibody NB100-2688 (blue) and a matched isotype control NBP1-43319 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems). Image from the standard format of this antibody.

Flow Cytometry: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] - A cell surface stain was performed on SK-MEL-28 cells with NG2/MCSP antibody (LHM 2) NB100-2688AF700 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 700.

Western Blot: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NG2/MCSP (LHM2) knockout (KO) HeLa cell line. PVDF membrane was probed with 1.0 ug/ml of Mouse Anti-Human NG2/MCSP (LHM2) Polyclonal Antibody (Catalog # NB100-2688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for NG2/MCSP (LHM2) at approximately 300 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions. Image from the standard format of this antibody.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IP, KO
• Clone: LHM 2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

NG2/MCSP Antibody (LHM 2) - Azide and BSA Free Summary

• Immunogen: A 375P cells crude extract
• Localization: Cell Membrane
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSPG4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface) 1 - 2.5 ug/ml
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10 - 1:100
  • Knockout Validated
  • Western Blot 1:1000
• Application Notes: In WB a band can be seen at ~300 kDa.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine, 0.15 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for NG2/MCSP Antibody (LHM 2) - Azide and BSA Free

• AN2
• chondroitin sulfate proteoglycan 4 (melanoma-associated)
• chondroitin sulfate proteoglycan 4
• CSPG4
• EC 3.6.3
• HMW-MAA
• MCSP
• MCSPChondroitin sulfate proteoglycan NG2
• MCSPG
• MCSPGMelanoma chondroitin sulfate proteoglycan
• MEL-CSPG
• MSK16Melanoma-associated chondroitin sulfate proteoglycan
• NG2
• NG2EC 2.7.8

Background

NG2 (Neuron Glial Antigen 2), also known as MCSP, and CSPG4 is a chondroitin surface proteoglycan. Chondroitin surface proteoglycans play a role in stabilizing cell-substratum interactions during early events of cell spreading. At large, proteoglycans are important for cell proliferation and migration, and may act as signal transducers through C-terminal cytoplasmic binding to scaffolding or signaling proteins. The NG2 glycoprotein is a type I membrane protein expressed in the developing and adult central nervous system (CNS) by subpopulations of glia including oligodendroglial precursor cells (OPCs), and in the developing CNS additionally by pericytes. NG2 is located at the apical cell membrane, however it has also been shown to localize to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Research has found that NG2 may inhibit neuronal growth (PMC1571583), and also acts as a proapoptotic receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NG2/MCSP Antibody (NBP2-80873)(1)

Publication using NBP2-80873

• Koivunotko E, Snirvi J, Merivaara A Et al. Angiogenic Potential of Human Adipose-Derived Mesenchymal Stromal Cells in Nanofibrillated Cellulose Hydrogel Biomedicines 2022-10-27 [PMID: 36289846] (WB, Human)
  
  Details:
  Citation using the Azide and BSA Free version of this antibody.

Bioinformatics

• Gene Symbol: CSPG4