MOG Antibody [Unconjugated]

MOG was detected in perfusion fixed frozen sections of mouse brain (cortex) using 15 µg/mL Goat Anti-Mouse MOG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2439) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MOG Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse MOG
  Gly29-Gly153
  Accession # Q61885
• Specificity: Detects mouse MOG in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant human MOG is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MOG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MOG Antibody [Unconjugated]

• MGC26137
• MOG Ig-AluB
• MOG
• MOGIG2
• myelin oligodendrocyte glycoprotein
• myelin-oligodendrocyte glycoprotein

Background

Mouse MOG is an integral membrane protein belonging to the immunoglobulin superfamily. It is found exclusively on myelinating oligodendrocytes in the central nervous system. The amino-terminal extracellular domain of mouse MOG shares 97% and 92% amino acid sequence identity with the corresponding regions of rat and human MOG, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MOG Antibody (AF2439)(4)

Publications using AF2439

• Colakoglu G, Bergstrom-Tyrberg U, Berglund E, Ranscht B Contactin-1 regulates myelination and nodal/paranodal domain organization in the central nervous system. Proc Natl Acad Sci U S A, 2014;111(3):E394-403. 2014 [PMID: 24385581] (IHC, Mouse)
• Ahn, Bum Ju, Le, Hoang, Shin, Min Wook, Bae, Sung-Jin, Lee, Eun Ji, Wee, Hee-Jun, Cha, Jong-Ho, Lee, Hyo-Jong, Lee, Hye Shin, Kim, Jeong Hu, Kim, Chang-Ye, Seo, Ji Hae, Lo, Eng H, Jeon, Sejin, Lee, Mi-Ni, Oh, Goo Taeg, Yin, Guo Nan, Ryu, Ji-Kan, Suh, Jun-Kyu, Kim, Kyu-Won Ninjurin1 deficiency attenuates susceptibility of experimental autoimmune encephalomyelitis in mice. J Biol Chem, 2014;289(6):3328-38. 2014 [PMID: 24347169] (IHC, Mouse)
• Di Maio A, Skuba A, Himes BT In vivo imaging of dorsal root regeneration: rapid immobilization and presynaptic differentiation at the CNS/PNS border. J. Neurosci., 2011;31(12):4569-82. 2011 [PMID: 21430157] (ICC, Mouse)
• Xi H, Katschke KJ, Helmy KY, Wark PA, Kljavin N, Clark H, Eastham-Anderson J, Shek T, Roose-Girma M, Ghilardi N, van Lookeren Campagne M Negative regulation of autoimmune demyelination by the inhibitory receptor CLM-1. J. Exp. Med., 2010;207(1):7-16, S1-5. 2010 [PMID: 20038601] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: MOG
• Uniprot:
  • Q61885()