NG2/MCSP Antibody (LHM 2) [PE]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
LHM 2
Clonality
Monoclonal
Host
Mouse
Conjugate
PE

NG2/MCSP Antibody (LHM 2) [PE] Summary

Immunogen
A 375P cells crude extract
Localization
Cell Membrane
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CSPG4
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
This NG2 Antibody (LHM 2) is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on both frozen and paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB, a band can be seen at ~300 kDa.

Reactivity Notes

Human. Other species have not been tested.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for NG2/MCSP Antibody (LHM 2) [PE]

  • AN2
  • chondroitin sulfate proteoglycan 4 (melanoma-associated)
  • chondroitin sulfate proteoglycan 4
  • CSPG4
  • EC 3.6.3
  • HMW-MAA
  • MCSP
  • MCSPChondroitin sulfate proteoglycan NG2
  • MCSPG
  • MCSPGMelanoma chondroitin sulfate proteoglycan
  • MEL-CSPG
  • MSK16Melanoma-associated chondroitin sulfate proteoglycan
  • NG2
  • NG2EC 2.7.8

Background

NG2 (Neuron Glial Antigen 2), also known as MCSP, and CSPG4 is a chondroitin surface proteoglycan. Chondroitin surface proteoglycans play a role in stabilizing cell-substratum interactions during early events of cell spreading. At large, proteoglycans are important for cell proliferation and migration, and may act as signal transducers through C-terminal cytoplasmic binding to scaffolding or signaling proteins. The NG2 glycoprotein is a type I membrane protein expressed in the developing and adult central nervous system (CNS) by subpopulations of glia including oligodendroglial precursor cells (OPCs), and in the developing CNS additionally by pericytes. NG2 is located at the apical cell membrane, however it has also been shown to localize to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Research has found that NG2 may inhibit neuronal growth (PMC1571583), and also acts as a proapoptotic receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-2688APC
DyLight 350 Labeled NB100-2688UV
DyLight 405 Labeled NB100-2688V
DyLight 488 Labeled NB100-2688G
DyLight 680 Labeled NB100-2688FR
DyLight 755 Labeled NB100-2688IR
FITC Labeled NB100-2688F
PerCP Labeled NB100-2688PCP

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Bioinformatics

Gene Symbol CSPG4
Entrez