Olig2 Antibody [Unconjugated]

A172 human glioblastoma cell line were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Olig2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The p21 promoter was detected by standard PCR.

Olig2 and Oligodendrocyte Marker O4 were detected in 7 day differentiated rat cortical stem cells using 10 µg/mL Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) and 10 µg/mL Goat Anti-Human/Mouse/Rat/Chicken O4 Monoclonal Antibody (Catalog # MAB1326). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Olig2 using the NorthernLights™ 637-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL002), and stained for O4 using an anti-mouse IgM secondary antibody (pseudo-stained green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Olig2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, ChIP, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Olig2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Olig2
  Met1-Lys323
  Accession # Q13516
• Specificity: Detects human Olig2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Olig3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: OLIG2

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Olig2 Antibody [Unconjugated]

• BHLHB1
• BHLHE19
• Class B basic helix-loop-helix protein 1
• Class E basic helix-loop-helix protein 19
• human protein kinase C-binding protein RACK17
• Olig2
• OLIGO2
• oligodendrocyte lineage transcription factor 2
• oligodendrocyte transcription factor 2
• oligodendrocyte-specific bHLH transcription factor 2
• PRKCBP2
• protein kinase C binding protein 2
• Protein kinase C-binding protein 2
• Protein kinase C-binding protein RACK17
• RACK17
• RACK17helix-loop-helix protein, class B, 1

Background

Olig1 and Olig2 are basic-helix-loop-helix (bHLH) transcription factors expressed in the motor neuron progenitor (pMN) domain of the spinal cord that generates motor neurons and oligodendrocytes. Olig1 is involved in the development and maturation of oligodendrocytes. Olig2 is required for oligodendrocyte and motor neuron specification in the spinal cord.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Olig2 Antibody (AF2418)(29)

Publications using AF2418

• T Srivastava, P Diba, JM Dean, F Banine, D Shaver, M Hagen, X Gong, W Su, B Emery, DL Marks, EN Harris, B Baggenstos, PH Weigel, LS Sherman, SA Back A TLR/AKT/FoxO3 immune tolerance-like pathway disrupts the repair capacity of oligodendrocyte progenitors J. Clin. Invest., 2018;0(0):. 2018 [PMID: 29664021] (IHC,, Rat)
• W Zhou, C Chen, Y Shi, Q Wu, RC Gimple, X Fang, Z Huang, K Zhai, SQ Ke, YF Ping, H Feng, JN Rich, JS Yu, S Bao, XW Bian Targeting Glioma Stem Cell-Derived Pericytes Disrupts the Blood-Tumor Barrier and Improves Chemotherapeutic Efficacy Cell Stem Cell, 2017;21(5):591-603.e4. 2017 [PMID: 29100012] (IHC,, Human)
• FI Young, M Keruzore, X Nan, N Gennet, EJ Bellefroid, M Li The doublesex-related Dmrta2 safeguards neural progenitor maintenance involving transcriptional regulation of Hes1 Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28655839] (ICC,, Mouse)
• NJ Yates, MK Giacci, RL O'Hare Doi, W Chiha, BE Ashworth, J Kenna, CA Bartlett, M Fitzgerald Delayed treatment of secondary degeneration following acute optic nerve transection using a combination of ion channel inhibitors Neural Regen Res, 2017;12(2):307-316. 2017 [PMID: 28400815] (IHC OCT-embedded,, Rat)
• Brain tissue banking for stem cells for our future Sci Rep, 2016;6(0):39394. 2016 [PMID: 27991551] (IHC Not Specified,, Human)
• Majistor Raj Luxman Maglo Renkilaraj The intellectual disability protein PAK3 regulates oligodendrocyte precursor cell differentiation Neurobiol. Dis, 2016;0(0):. 2016 [PMID: 27940202] (ICC,, Mouse)
• A cord blood monocyte-derived cell therapy product accelerates brain remyelination JCI Insight, 2016;1(13):e86667. 2016 [PMID: 27699230] (IHC Not Specified,, Mouse)
• Patched1 and Patched2 inhibit Smoothened non-cell autonomously Elife, 2016;5(0):. 2016 [PMID: 27552050] (ICC Fluorescence,, Mouse)
• Qi Xie RBPJ maintains brain tumor-initiating cells through CDK9-mediated transcriptional elongation J Clin Invest, 2016;126(7):2757-2772. 2016 [PMID: 27322055] (WB,, Human)
• Olig2 is expressed late in human eosinophil development and controls Siglec-8 expression J Leukoc Biol, 2016;0(0):. 2016 [PMID: 27154355] (ChIP Assay,, Human)
• SH Choi, SH Kim, KW Shim, JW Han, J Choi, DS Kim, CJ Lyu, JW Kim, CO Suh, J Cho Treatment Outcome and Prognostic Molecular Markers of Supratentorial Primitive Neuroectodermal Tumors PLoS ONE, 2016;11(4):e0153443. 2016 [PMID: 27074032] (IHC Paraffin-embedded,, Human)
• Mendes F, Coelho Aguiar J, Kahn S, Reis A, Dubois L, Romao L, Ferreira L, Chneiweiss H, Moura Neto V, Abreu J Connective-Tissue Growth Factor (CTGF/CCN2) Induces Astrogenesis and Fibronectin Expression of Embryonic Neural Cells In Vitro. PLoS ONE, 0;10(8):e0133689. 0 [PMID: 26241738] (NotWB,, Human)
• Rzem R, Achouri Y, Marbaix E, Schakman O, Wiame E, Marie S, Gailly P, Vincent M, Veiga-da-Cunha M, Van Schaftingen E A mouse model of L-2-hydroxyglutaric aciduria, a disorder of metabolite repair. PLoS ONE, 2015;10(3):e0119540. 2015 [PMID: 25763823] (IHC OCT,, Mouse)
• Bergsland M, Covacu R, Perez Estrada C, Svensson M, Brundin L Nitric oxide-induced neuronal to glial lineage fate-change depends on NRSF/REST function in neural progenitor cells. Stem Cells, 2014;32(9):2539-49. 2014 [PMID: 24807147] (IHC,, Rat)
• Yan K, Wu Q, Yan D, Lee C, Rahim N, Tritschler I, DeVecchio J, Kalady M, Hjelmeland A, Rich J Glioma cancer stem cells secrete Gremlin1 to promote their maintenance within the tumor hierarchy. Genes Dev, 2014;28(10):1085-100. 2014 [PMID: 24788093] (IF,, Human)
• Srikanth, Maya, Kim, Juno, Das, Sunit, Kessler, John A BMP signaling induces astrocytic differentiation of clinically derived oligodendroglioma propagating cells. Mol Cancer Res, 2014;12(2):283-94. 2014 [PMID: 24269952] (IHC Frozen,, Human)
• Piltti K, Salazar D, Uchida N, Cummings B, Anderson A Safety of epicenter versus intact parenchyma as a transplantation site for human neural stem cells for spinal cord injury therapy. Stem Cells Transl Med, 2013;2(3):204-16. 2013 [PMID: 23413374] (ICC,, Human)
• Salazar DL, Uchida N, Hamers FP, Cummings BJ, Anderson AJ Human neural stem cells differentiate and promote locomotor recovery in an early chronic spinal cord injury NOD-scid mouse model. PLoS ONE, 2010;5(8):e12272. 2010 [PMID: 20806064] (ICC,, Human)
• Bauer S, Patterson PH Leukemia inhibitory factor promotes neural stem cell self-renewal in the adult brain. J. Neurosci., 2006;26(46):12089-99. 2006 [PMID: 17108182] (IHC Frozen,, Mouse)
• Xie Q, Wu Q, Horbinski C, Flavahan W, Yang K, Zhou W, Dombrowski S, Huang Z, Fang X, Shi Y, Ferguson A, Kashatus D, Bao S, Rich J Mitochondrial control by DRP1 in brain tumor initiating cells. Nat Neurosci, 2015;18(4):501-10. 2015 [PMID: 25730670] (WB,, Human)
• Ben-Shushan E, Feldman E, Reubinoff B Notch signaling regulates motor neuron differentiation of human embryonic stem cells. Stem Cells, 2015;33(2):403-15. 2015 [PMID: 25335858] (ICC Fluorescence,, Human)
• Liu, J K, Lubelski, D, Schonberg, D L, Wu, Q, Hale, J S, Flavahan, W A, Mulkearns-Hubert, E E, Man, J, Hjelmeland, A B, Yu, J, Lathia, J D, Rich, J N Phage display discovery of novel molecular targets in glioblastoma-initiating cells. Cell Death Differ, 2014;21(8):1325-39. 2014 [PMID: 24832468] (WB,, Human)
• Seto Y, Nakatani T, Masuyama N, Taya S, Kumai M, Minaki Y, Hamaguchi A, Inoue Y, Inoue T, Miyashita S, Fujiyama T, Yamada M, Chapman H, Campbell K, Magnuson M, Wright C, Kawaguchi Y, Ikenaka K, Takebayashi H, Ishiwata S, Ono Y, Hoshino M Temporal identity transition from Purkinje cell progenitors to GABAergic interneuron progenitors in the cerebellum. Nat Commun, 2014;5(0):3337. 2014 [PMID: 24535035] (IHC - PFA fixed,, Mouse)
• Kuboyama, Kazuya, Fujikawa, Akihiro, Masumura, Makoto, Suzuki, Ryoko, Matsumoto, Masahito, Noda, Masaharu Protein tyrosine phosphatase receptor type z negatively regulates oligodendrocyte differentiation and myelination. PLoS ONE, 2012;7(11):e48797. 2012 [PMID: 23144976] (ICC,, Mouse)
• Zelinka C, Scott M, Volkov L, Fischer A The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1. PLoS ONE, 2012;7(9):e44477. 2012 [PMID: 22973454] (IHC,, Chicken)
• Martinez-Cerdeno V, Lemen JM, Chan V, Wey A, Lin W, Dent SR, Knoepfler PS N-Myc and GCN5 regulate significantly overlapping transcriptional programs in neural stem cells. PLoS ONE, 2012;7(6):e39456. 2012 [PMID: 22745758] (IHC Paraffin-embedded,, Mouse)
• Vue TY, Aaker J, Taniguchi A Characterization of progenitor domains in the developing mouse thalamus. J. Comp. Neurol., 2007;505(1):73-91. 2007 [PMID: 17729296] (IHC Frozen,, Mouse)
• Widestrand A, Faijerson J, Wilhelmsson U, Smith PL, Li L, Sihlbom C, Eriksson PS, Pekny M Increased neurogenesis and astrogenesis from neural progenitor cells grafted in the hippocampus of GFAP-/- Vim-/- mice. Stem Cells, 2007;25(10):2619-27. 2007 [PMID: 17628017] (ICC,, Rat)

Review for Olig2 Antibody (AF2418) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Anonymous ICC Mouse 01/06/2015

Summary

• Application: Immunocytochemistry
• Sample Tested: See PMID 22504067
• Species: Mouse

Blocking

• Blocking Details: See PMID 22504067

Details

• Detection Notes: See PMID 22504067

Comments

• Comments: Also used immunohistochemistry frozenPublished. See PMID 22504067

Bioinformatics

• Gene Symbol: OLIG2
• Entrez:
  • 10215(Human)
• Uniprot:
  • Q13516()