Olig2 Antibody [Unconjugated]

Images

 
Olig2 and Oligodendrocyte Marker O4 were detected in 7 day differentiated rat cortical stem cells using 10 µg/mL Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) and 10 ...read more
A172 human glioblastoma cell line were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Olig2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse/Rat Olig2 ...read more
Olig2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) at 8 µg/mL overnight at 4 °C. ...read more
Olig2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) at 10 µg/mL for ...read more
Olig2 was detected in immersion fixed paraffin-embedded sections of rat midbrain using Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) at 10 µg/mL for 1 hour ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Olig2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Olig2
Met1-Lys323
Accession # Q13516
Specificity
Detects human Olig2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
OLIG2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF2418 in the following applications:

Publications
Read Publications using
AF2418 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Olig2 Antibody [Unconjugated]

  • BHLHB1
  • BHLHE19
  • Class B basic helix-loop-helix protein 1
  • Class E basic helix-loop-helix protein 19
  • human protein kinase C-binding protein RACK17
  • Olig2
  • OLIGO2
  • oligodendrocyte lineage transcription factor 2
  • oligodendrocyte transcription factor 2
  • oligodendrocyte-specific bHLH transcription factor 2
  • PRKCBP2
  • protein kinase C binding protein 2
  • Protein kinase C-binding protein 2
  • Protein kinase C-binding protein RACK17
  • RACK17
  • RACK17helix-loop-helix protein, class B, 1

Background

Olig1 and Olig2 are basic-helix-loop-helix (bHLH) transcription factors expressed in the motor neuron progenitor (pMN) domain of the spinal cord that generates motor neurons and oligodendrocytes. Olig1 is involved in the development and maturation of oligodendrocytes. Olig2 is required for oligodendrocyte and motor neuron specification in the spinal cord.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Olig2 Antibody (AF2418)(98)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chicken, Hamster - Mesocricetus auratus (Golden Hamster), Transgenic Mouse.

We have publications tested in 12 applications: Bioassay, ChIP, Co-Immunoprecipitation, FISH, Flow Cytometry, ICC, ICC/IF, IHC, IHC-Fr, IHC-P, IHC/IF, Western Blot.


Filter By Application
Bioassay
(1)
ChIP
(1)
Co-Immunoprecipitation
(1)
FISH
(1)
Flow Cytometry
(2)
ICC
(20)
ICC/IF
(1)
IHC
(60)
IHC-Fr
(7)
IHC-P
(2)
IHC/IF
(1)
Western Blot
(9)
All Applications
Filter By Species
Human
(35)
Mouse
(47)
Rat
(14)
Chicken
(2)
Hamster - Mesocricetus auratus (Golden Hamster)
(1)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 98. Show All 98 Publications.
Publications using AF2418 Applications Species
Baeza-Kallee, N;Bergès, R;Hein, V;Cabaret, S;Garcia, J;Gros, A;Tabouret, E;Tchoghandjian, A;Colin, C;Figarella-Branger, D; Deciphering the Action of Neuraminidase in Glioblastoma Models International journal of molecular sciences 2023-07-19 [PMID: 37511403] (ICC/IF, Mouse) ICC/IF Mouse
Mätlik, K;Baffuto, M;Kus, L;Deshmukh, AL;Davis, DA;Paul, MR;Carroll, TS;Caron, MC;Masson, JY;Pearson, CE;Heintz, N; Cell Type Specific CAG Repeat Expansions and Toxicity of Mutant Huntingtin in Human Striatum and Cerebellum bioRxiv : the preprint server for biology 2023-06-08 [PMID: 37333326] (Flow Cytometry, Western Blot, Human) Flow Cytometry, Western Blot Human
Maruyama, T;Tanabe, S;Uyeda, A;Suzuki, T;Muramatsu, R; Free fatty acids support oligodendrocyte survival in a mouse model of amyotrophic lateral sclerosis Frontiers in cellular neuroscience 2023-05-12 [PMID: 37252191] (IHC, Mouse) IHC Mouse
Seeker, LA;Bestard-Cuche, N;J�kel, S;Kazakou, NL;B�strand, SMK;Wagstaff, LJ;Cholewa-Waclaw, J;Kilpatrick, AM;Van Bruggen, D;Kabbe, M;Baldivia Pohl, F;Moslehi, Z;Henderson, NC;Vallejos, CA;La Manno, G;Castelo-Branco, G;Williams, A; Brain matters: unveiling the distinct contributions of region, age, and sex to glia diversity and�CNS function Acta neuropathologica communications 2023-05-22 [PMID: 37217978] (IHC, Human) IHC Human
Pressl, C;M�tlik, K;Kus, L;Darnell, P;Luo, JD;Weiss, AR;Liguore, W;Carroll, TS;Davis, DA;McBride, J;Heintz, N; Layer 5a Corticostriatal Projection Neurons are Selectively Vulnerable in Huntington's Disease bioRxiv : the preprint server for biology 2023-04-24 [PMID: 37162977] (ICC, Human) ICC Human
Y Wang, D Sadike, B Huang, P Li, Q Wu, N Jiang, Y Fang, G Song, L Xu, W Wang, M Xie Regulatory T cells alleviate myelin loss and cognitive dysfunction by regulating neuroinflammation and microglial pyroptosis via TLR4/MyD88/NF-kappaB pathway in LPC-induced demyelination Journal of Neuroinflammation, 2023-02-18;20(1):41. 2023-02-18 [PMID: 36803990] (IHC, Mouse) IHC Mouse
H Park, B Cho, H Kim, T Saito, TC Saido, KJ Won, J Kim Single-cell RNA-sequencing identifies disease-associated oligodendrocytes in male APP NL-G-F and 5XFAD mice Nature Communications, 2023-02-13;14(1):802. 2023-02-13 [PMID: 36781874] (Flow Cytometry, Mouse) Flow Cytometry Mouse
M Ferren, A Lalande, M Iampietro, L Canus, D Decimo, D Gerlier, M Porotto, C Mathieu Early Permissiveness of Central Nervous System Cells to Measles Virus Infection Is Determined by Hyperfusogenicity and Interferon Pressure Viruses, 2023-01-13;15(1):. 2023-01-13 [PMID: 36680268] (Bioassay, Hamster - Mesocricetus auratus (Golden Hamster)) Bioassay Hamster - Mesocricetus auratus (Golden Hamster)
S Chen, Y Chang, L Li, D Acosta, Y Li, Q Guo, C Wang, E Turkes, C Morrison, D Julian, ME Hester, DW Scharre, C Santiskulv, SX Song, JT Plummer, GE Serrano, TG Beach, KE Duff, Q Ma, H Fu Spatially resolved transcriptomics reveals genes associated with the vulnerability of middle temporal gyrus in Alzheimer's disease Acta neuropathologica communications, 2022-12-21;10(1):188. 2022-12-21 [PMID: 36544231] (IHC, Human) IHC Human
Show All 98 Publications.

Reviews for Olig2 Antibody (AF2418) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF2418:
Filter by Applications
IHC-Fr
(1)
ICC
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Olig2 AF2418
Enlarge
5
reviewed by:
Xuecheng Qiu
IHC-Fr Mouse 05/12/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse brain
SpeciesMouse
LotUPA0617051
  4
reviewed by:
Verified Customer
ICC Mouse 01/06/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 22504067
SpeciesMouse

Product General Protocols

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FAQs for Olig2 Antibody (AF2418) (0)

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Secondary Antibodies

 

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Xuecheng Qiu
05/12/2019
Application: IHC-Fr
Species: Mouse

 
Verified Customer
01/06/2015
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol OLIG2
Uniprot