Olig2 Antibody [Unconjugated]

A172 human glioblastoma cell line were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Olig2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The p21 promoter was detected by standard PCR.

Olig2 and Oligodendrocyte Marker O4 were detected in 7 day differentiated rat cortical stem cells using 10 µg/mL Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) and 10 µg/mL Goat Anti-Human/Mouse/Rat/Chicken O4 Monoclonal Antibody (Catalog # MAB1326). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Olig2 using the NorthernLights™ 637-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL002), and stained for O4 using an anti-mouse IgM secondary antibody (pseudo-stained green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Olig2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, ChIP, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Olig2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Olig2
  Met1-Lys323
  Accession # Q13516
• Specificity: Detects human Olig2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: OLIG2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Olig2 Antibody [Unconjugated]

• BHLHB1
• BHLHE19
• Class B basic helix-loop-helix protein 1
• Class E basic helix-loop-helix protein 19
• human protein kinase C-binding protein RACK17
• Olig2
• OLIGO2
• oligodendrocyte lineage transcription factor 2
• oligodendrocyte transcription factor 2
• oligodendrocyte-specific bHLH transcription factor 2
• PRKCBP2
• protein kinase C binding protein 2
• Protein kinase C-binding protein 2
• Protein kinase C-binding protein RACK17
• RACK17
• RACK17helix-loop-helix protein, class B, 1

Background

Olig1 and Olig2 are basic-helix-loop-helix (bHLH) transcription factors expressed in the motor neuron progenitor (pMN) domain of the spinal cord that generates motor neurons and oligodendrocytes. Olig1 is involved in the development and maturation of oligodendrocytes. Olig2 is required for oligodendrocyte and motor neuron specification in the spinal cord.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Olig2 Antibody (AF2418)(68)

Publications using AF2418

• C Azevedo, G Teku, Y Pomeshchik, JF Reyes, M Chumarina, K Russ, E Savchenko, A Hammarberg, NJ Lamas, A Collin, GK Gouras, O Klementiev, M Hallbeck, R Taipa, M Vihinen, L Roybon Parkinson's disease and multiple system atrophy patient iPSC-derived oligodendrocytes exhibit alpha-synuclein-induced changes in maturation and immune reactive properties Proceedings of the National Academy of Sciences of the United States of America, 2022;119(12):e2111405119. 2022 [PMID: 35294277] (ICC, Human)
• T Feng, L Luan, II Katz, M Ullah, VM Van Deerli, JQ Trojanowsk, EB Lee, F Hu TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss Acta neuropathologica communications, 2022;10(1):33. 2022 [PMID: 35287730] (Western Blot, Mouse)
• WU Park, GB Yeon, MS Yu, HG Goo, SH Hwang, D Na, DS Kim A Novel Vitronectin Peptide Facilitates Differentiation of Oligodendrocytes from Human Pluripotent Stem Cells (Synthetic ECM for Oligodendrocyte Differentiation) Biology, 2021;10(12):. 2021 [PMID: 34943169] (ICC, Human)
• LM Toomey, M Papini, B Lins, AJ Wright, A Warnock, T McGonigle, SC Hellewell, CA Bartlett, C Anyaegbu, M Fitzgerald Cuprizone feed formulation influences the extent of demyelinating disease pathology Scientific Reports, 2021;11(1):22594. 2021 [PMID: 34799634] (IHC, Mouse)
• Y Kimura, Y Hayashi, S Hitomi, D Ikutame, K Urata, I Shibuta, A Sakai, J Ni, K Iwata, M Tonogi, M Shinoda IL-33 induces orofacial neuropathic pain through Fyn-dependent phosphorylation of GluN2B in the trigeminal spinal nucleus caudalis Brain, Behavior, and Immunity, 2021;0(0):. 2021 [PMID: 34715301] (IHC, Mouse)
• Y Takanezawa, S Tanabe, D Kato, R Ozeki, M Komoda, T Suzuki, H Baba, R Muramatsu Microglial ASD-related genes are involved in oligodendrocyte differentiation Scientific Reports, 2021;11(1):17825. 2021 [PMID: 34497307] (IHC, Mouse)
• LE Blizzard, C Menke, SD Patel, RR Waclaw, SA Lachke, RW Stottmann A Novel Mutation in Cse1l Disrupts Brain and Eye Development with Specific Effects on Pax6 Expression Journal of developmental biology, 2021;9(3):. 2021 [PMID: 34287339] (IHC, Mouse)
• M Connor, K Lamorie-Fo, Q Liu, K Shkirkova, H Baertsch, C Sioutas, TE Morgan, CE Finch, WJ Mack Nanoparticulate matter exposure results in white matter damage and an inflammatory microglial response in an experimental murine model PLoS ONE, 2021;16(7):e0253766. 2021 [PMID: 34214084] (IHC, Mouse)
• T Zhang, T Liu, N Mora, J Guegan, M Bertrand, X Contreras, AH Hansen, C Streicher, M Anderle, N Danda, L Tiberi, S Hippenmeye, BA Hassan Generation of excitatory and inhibitory neurons from common progenitors via Notch signaling in the cerebellum Cell Reports, 2021;35(10):109208. 2021 [PMID: 34107249] (IHC, Mouse)
• SM Nam, JH Choi, SH Choi, HJ Cho, YJ Cho, H Rhim, HC Kim, IH Cho, DG Kim, SY Nah Ginseng gintonin alleviates neurological symptoms in the G93A-SOD1 transgenic mouse model of amyotrophic lateral sclerosis through lysophosphatidic acid 1 receptor Journal of Ginseng Research, 2021;45(3):390-400. 2021 [PMID: 34025132] (IHC, Mouse)
• P Filppu, JT Ramanathan, KJ Granberg, E Gucciardo, H Haapasalo, K Lehti, M Nykter, V Le Joncour, P Laakkonen CD109-GP130 interaction drives glioblastoma stem cell plasticity and chemoresistance through STAT3 activity JCI Insight, 2021;6(9):. 2021 [PMID: 33986188] (Co-Immunoprecipitation, Human)
• W Mu, S Li, J Xu, X Guo, H Wu, Z Chen, L Qiao, G Helfer, F Lu, C Liu, QF Wu Hypothalamic Rax+ tanycytes contribute to tissue repair and tumorigenesis upon oncogene activation in mice Nature Communications, 2021;12(1):2288. 2021 [PMID: 33863883] (IHC, Mouse)
• JE Lee, S Ahn, H Jeong, S An, CH Myung, JA Lee, SC Hong, YJ Kim, JY Kim, JH Ryu, M Noh, KT Nam, JS Hwang Olig2 regulates p53-mediated apoptosis, migration and invasion of melanoma cells Scientific Reports, 2021;11(1):7778. 2021 [PMID: 33833342] (Western Blot, Human)
• C Ogawa, S Mikawa, Y Hayashi, K Masumoto, F Katou, K Sato BMP9 expression in the adult rat brain Journal of chemical neuroanatomy, 2021;113(0):101933. 2021 [PMID: 33582251] (IHC, Rat)
• BY Fan, YL Pang, WX Li, CX Zhao, Y Zhang, X Wang, GZ Ning, XH Kong, C Liu, X Yao, SQ Feng Liproxstatin-1 is an effective inhibitor of oligodendrocyte ferroptosis induced by inhibition of glutathione peroxidase 4 Neural regeneration research, 2021;16(3):561-566. 2021 [PMID: 32985488] (ICC, Human)
• AL Koessinger, D Koessinger, K Stevenson, C Cloix, L Mitchell, C Nixon, N Gomez-Roma, AJ Chalmers, JC Norman, SWG Tait Quantitative in vivo bioluminescence imaging of orthotopic patient-derived glioblastoma xenografts Scientific Reports, 2020;10(1):15361. 2020 [PMID: 32958777] (Western Blot, Human)
• W Wang, H Cho, D Kim, Y Park, JH Moon, SJ Lim, SM Yoon, M McCane, SA Aicher, S Kim, B Emery, JW Lee, S Lee, Y Park, SK Lee PRC2 Acts as a Critical Timer That Drives Oligodendrocyte Fate over Astrocyte Identity by Repressing the Notch Pathway Cell Rep, 2020;32(11):108147. 2020 [PMID: 32937136] (IHC, Chicken)
• MJ Borrett, BT Innes, D Jeong, N Tahmasian, MA Storer, GD Bader, DR Kaplan, FD Miller Single-Cell Profiling Shows Murine Forebrain Neural Stem Cells Reacquire a Developmental State when Activated for Adult Neurogenesis Cell Rep, 2020;32(6):108022. 2020 [PMID: 32783944] (FISH, Mouse)
• S Kaji, T Maki, J Ueda, T Ishimoto, Y Inoue, K Yasuda, M Sawamura, R Hikawa, T Ayaki, H Yamakado, R Takahashi BCAS1-positive immature oligodendrocytes are affected by the &alpha-synuclein-induced pathology of multiple system atrophy Acta Neuropathol Commun, 2020;8(1):120. 2020 [PMID: 32727582] (IHC, Rat)
• M Duman, A Vaquié, G Nocera, M Heller, M Stumpe, D Siva Sanka, J Dengjel, D Meijer, T Yamaguchi, P Matthias, T Zeis, N Schaeren-W, A Hayoz, S Ruff, C Jacob EEF1A1 deacetylation enables transcriptional activation of remyelination Nat Commun, 2020;11(1):3420. 2020 [PMID: 32647127] (ICC, Mouse)
• LM Ruiz-Perer, JFW Greiner, C Kaltschmid, B Kaltschmid A Matter of Choice: Inhibition of c-Rel Shifts Neuronal to Oligodendroglial Fate in Human Stem Cells Cells, 2020;9(4):. 2020 [PMID: 32331232] (ICC, Human)
• TC Ma, KI Vong, KM Kwan Spatiotemporal Decline of BMP Signaling Activity in Neural Progenitors Mediates Fate Transition and Safeguards Neurogenesis Cell Rep, 2020;30(11):3616-3624.e4. 2020 [PMID: 32187534] (IHC, Mouse)
• SM Nam, SH Choi, HJ Cho, JS Seo, M Choi, SS Nahm, BJ Chang, SY Nah Ginseng Gintonin Attenuates Lead-Induced Rat Cerebellar Impairments during Gestation and Lactation Biomolecules, 2020;10(3):. 2020 [PMID: 32131481] (IHC, Rat)
• M Fares, M Cochet-Ber, G Gonzalez, CN Montero-Me, O Blanchet, A Benchoua, C Boissart, S Lecollinet, J Richardson, N Haddad, M Coulpier Pathological modeling of TBEV infection reveals differential innate immune responses in human neurons and astrocytes that correlate with their susceptibility to infection J Neuroinflammation, 2020;17(1):76. 2020 [PMID: 32127025] (ICC, Human)
• C Ogawa, S Mikawa, K Yamashita, Y Hayashi, K Masumoto, F Katou, K Sato Follistatin expression in the central nervous system of the adult rat J. Chem. Neuroanat., 2020;105(0):101753. 2020 [PMID: 32014555] (IHC, Rat)
• Y Zhou, WM Song, PS Andhey, A Swain, T Levy, KR Miller, PL Poliani, M Cominelli, S Grover, S Gilfillan, M Cella, TK Ulland, K Zaitsev, A Miyashita, T Ikeuchi, M Sainouchi, A Kakita, DA Bennett, JA Schneider, MR Nichols, SA Beausoleil, JD Ulrich, DM Holtzman, MN Artyomov, M Colonna Human and mouse single-nucleus transcriptomics reveal TREM2-dependent and TREM2-independent cellular responses in Alzheimer's disease Nat. Med., 2020;26(1):131-142. 2020 [PMID: 31932797] (IHC, Mouse)
• LM Toomey, CA Bartlett, N Gavriel, T McGonigle, M Majimbi, G Gopalasing, J Rodger, M Fitzgerald Comparing modes of delivery of a combination of ion channel inhibitors for limiting secondary degeneration following partial optic nerve transection Sci Rep, 2019;9(1):15297. 2019 [PMID: 31653948] (IHC, Rat)
• S Clavreul, L Abdeladim, E Hernández-, D Niculescu, J Durand, SH Ieng, R Barry, G Bonvento, E Beaurepair, J Livet, K Loulier Cortical astrocytes develop in a plastic manner at both clonal and cellular levels Nat Commun, 2019;10(1):4884. 2019 [PMID: 31653848] (IHC, Mouse)
• M Boström, Y Eriksson, J Danial, T Björk-Erik, M Kalm Chronic disturbance in the thalamus following cranial irradiation to the developing mouse brain Sci Rep, 2019;9(1):9588. 2019 [PMID: 31270437] (IHC, Mouse)
• G Gopalasing, CA Bartlett, T McGonigle, M Majimbi, A Warnock, A Ford, A Gough, LM Toomey, M Fitzgerald The effects of a combination of ion channel inhibitors on pathology in a model of demyelinating disease Mult Scler Relat Disord, 2019;34(0):1-8. 2019 [PMID: 31202958] (IHC, Mouse)
• Y Hayashi, S Mikawa, C Ogawa, K Masumoto, F Katou, K Sato BMP6 expression in the adult rat central nervous system J. Chem. Neuroanat., 2019;0(0):. 2019 [PMID: 30928603] (IHC-Fr, Rat)
• V Bellver-La, F Bretheau, B Mailhot, N Vallières, M Lessard, ME Janelle, N Vernoux, MÈ Tremblay, T Fuehrmann, MS Shoichet, S Lacroix Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury Nat Commun, 2019;10(1):518. 2019 [PMID: 30705270] (IHC, Mouse)
• M Osipovitch, A Asenjo Mar, JN Mariani, A Cornwell, S Dhaliwal, L Zou, D Chandler-M, S Wang, X Li, SJ Benraiss, R Agate, A Lampp, A Benraiss, MS Windrem, SA Goldman Human ESC-Derived Chimeric Mouse Models of Huntington's Disease Reveal Cell-Intrinsic Defects in Glial Progenitor Cell Differentiation Cell Stem Cell, 2018;0(0):. 2018 [PMID: 30554964] (IHC, Human)
• AM Falcão, D van Brugge, S Marques, M Meijer, S Jäkel, E Agirre, Samudyata, EM Floriddia, DP Vanichkina, C Ffrench-Co, A Williams, AO Guerreiro-, G Castelo-Br Disease-specific oligodendrocyte lineage cells arise in multiple sclerosis Nat. Med., 2018;0(0):. 2018 [PMID: 30420755] (IHC-Fr, Mouse)
• LM Ruiz-Perer, L Schneider, BA Windmöller, J Müller, JFW Greiner, C Kaltschmid, B Kaltschmid NF-?B p65 directs sex-specific neuroprotection in human neurons Sci Rep, 2018;8(1):16012. 2018 [PMID: 30375448] (ICC, Human)
• Y Hayashi, S Mikawa, C Ogawa, K Masumoto, F Katou, K Sato Myostatin expression in the adult rat central nervous system J. Chem. Neuroanat., 2018;0(0):. 2018 [PMID: 30366029] (IHC, Rat)
• Q Chen, J Wu, Q Ye, F Ma, Q Zhu, Y Wu, C Shan, X Xie, D Li, X Zhan, C Li, XF Li, X Qin, T Zhao, H Wu, PY Shi, J Man, CF Qin Treatment of Human Glioblastoma with a Live Attenuated Zika Virus Vaccine Candidate MBio, 2018;9(5):. 2018 [PMID: 30228241] (IHC, Mouse)
• T Srivastava, P Diba, JM Dean, F Banine, D Shaver, M Hagen, X Gong, W Su, B Emery, DL Marks, EN Harris, B Baggenstos, PH Weigel, LS Sherman, SA Back A TLR/AKT/FoxO3 immune tolerance-like pathway disrupts the repair capacity of oligodendrocyte progenitors J. Clin. Invest., 2018;0(0):. 2018 [PMID: 29664021] (IHC, Rat)
• F Cathomas, D Azzinnari, G Bergamini, H Sigrist, M Buerge, V Hoop, B Wicki, L Goetze, S Soares, D Kukelova, E Seifritz, S Goebbels, KA Nave, M Said Ghand, C Seoighe, T Hildebrand, G Leparc, H Klein, E Stupka, B Hengerer, CR Pryce Oligodendrocyte gene expression is reduced by and influences effects of chronic social stress in mice Genes Brain Behav., 2018;0(0):e12475. 2018 [PMID: 29566304] (IHC-Fr, Mouse)
• M Datta, O Staszewski, E Raschi, M Frosch, N Hagemeyer, TL Tay, T Blank, M Kreutzfeld, D Merkler, S Ziegler-Wa, P Matthias, M Meyer-Lueh, M Prinz Histone Deacetylases 1 and 2 Regulate Microglia Function during Development, Homeostasis, and Neurodegeneration in a Context-Dependent Manner Immunity, 2018;0(0):. 2018 [PMID: 29548672] (IHC-Fr, Mouse)
• W Zhou, C Chen, Y Shi, Q Wu, RC Gimple, X Fang, Z Huang, K Zhai, SQ Ke, YF Ping, H Feng, JN Rich, JS Yu, S Bao, XW Bian Targeting Glioma Stem Cell-Derived Pericytes Disrupts the Blood-Tumor Barrier and Improves Chemotherapeutic Efficacy Cell Stem Cell, 2017;21(5):591-603.e4. 2017 [PMID: 29100012] (IHC, Human)
• FI Young, M Keruzore, X Nan, N Gennet, EJ Bellefroid, M Li The doublesex-related Dmrta2 safeguards neural progenitor maintenance involving transcriptional regulation of Hes1 Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28655839] (ICC, Mouse)
• NJ Yates, MK Giacci, RL O'Hare Doi, W Chiha, BE Ashworth, J Kenna, CA Bartlett, M Fitzgerald Delayed treatment of secondary degeneration following acute optic nerve transection using a combination of ion channel inhibitors Neural Regen Res, 2017;12(2):307-316. 2017 [PMID: 28400815] (IHC, Rat)
• Brain tissue banking for stem cells for our future Sci Rep, 2016;6(0):39394. 2016 [PMID: 27991551] (IHC, Human)
• Majistor Raj Luxman Maglo Renkilaraj The intellectual disability protein PAK3 regulates oligodendrocyte precursor cell differentiation Neurobiol. Dis, 2016;0(0):. 2016 [PMID: 27940202] (ICC, Mouse)
• A cord blood monocyte-derived cell therapy product accelerates brain remyelination JCI Insight, 2016;1(13):e86667. 2016 [PMID: 27699230] (IHC, Mouse)
• Patched1 and Patched2 inhibit Smoothened non-cell autonomously Elife, 2016;5(0):. 2016 [PMID: 27552050] (ICC, Mouse)
• Qi Xie RBPJ maintains brain tumor-initiating cells through CDK9-mediated transcriptional elongation J Clin Invest, 2016;126(7):2757-2772. 2016 [PMID: 27322055] (Western Blot, Human)
• Olig2 is expressed late in human eosinophil development and controls Siglec-8 expression J Leukoc Biol, 2016;0(0):. 2016 [PMID: 27154355] (ChIP, Human)
• SH Choi, SH Kim, KW Shim, JW Han, J Choi, DS Kim, CJ Lyu, JW Kim, CO Suh, J Cho Treatment Outcome and Prognostic Molecular Markers of Supratentorial Primitive Neuroectodermal Tumors PLoS ONE, 2016;11(4):e0153443. 2016 [PMID: 27074032] (IHC-P, Human)
• Mendes F, Coelho Aguiar J, Kahn S, Reis A, Dubois L, Romao L, Ferreira L, Chneiweiss H, Moura Neto V, Abreu J Connective-Tissue Growth Factor (CTGF/CCN2) Induces Astrogenesis and Fibronectin Expression of Embryonic Neural Cells In Vitro. PLoS ONE, 0;10(8):e0133689. 0 [PMID: 26241738] (NotWestern Blot, Human)
• Rzem R, Achouri Y, Marbaix E, Schakman O, Wiame E, Marie S, Gailly P, Vincent M, Veiga-da-Cunha M, Van Schaftingen E A mouse model of L-2-hydroxyglutaric aciduria, a disorder of metabolite repair. PLoS ONE, 2015;10(3):e0119540. 2015 [PMID: 25763823] (IHC, Mouse)
• Xie Q, Wu Q, Horbinski C, Flavahan W, Yang K, Zhou W, Dombrowski S, Huang Z, Fang X, Shi Y, Ferguson A, Kashatus D, Bao S, Rich J Mitochondrial control by DRP1 in brain tumor initiating cells. Nat Neurosci, 2015;18(4):501-10. 2015 [PMID: 25730670] (Western Blot, Human)
• Ben-Shushan E, Feldman E, Reubinoff B Notch signaling regulates motor neuron differentiation of human embryonic stem cells. Stem Cells, 2015;33(2):403-15. 2015 [PMID: 25335858] (ICC, Human)
• Liu , J K, Lubelski , D, Schonberg , D L, Wu , Q, Hale , J S, Flavahan , W A, Mulkearns-Hubert , E E, Man , J, Hjelmeland , A B, Yu , J, Lathia , J D, Rich , J N Phage display discovery of novel molecular targets in glioblastoma-initiating cells. Cell Death Differ, 2014;21(8):1325-39. 2014 [PMID: 24832468] (Western Blot, Human)
• Bergsland M, Covacu R, Perez Estrada C, Svensson M, Brundin L Nitric oxide-induced neuronal to glial lineage fate-change depends on NRSF/REST function in neural progenitor cells. Stem Cells, 2014;32(9):2539-49. 2014 [PMID: 24807147] (IHC, Rat)
• Yan K, Wu Q, Yan D, Lee C, Rahim N, Tritschler I, DeVecchio J, Kalady M, Hjelmeland A, Rich J Glioma cancer stem cells secrete Gremlin1 to promote their maintenance within the tumor hierarchy. Genes Dev, 2014;28(10):1085-100. 2014 [PMID: 24788093] (IHC, Human)
• Seto Y, Nakatani T, Masuyama N, Taya S, Kumai M, Minaki Y, Hamaguchi A, Inoue Y, Inoue T, Miyashita S, Fujiyama T, Yamada M, Chapman H, Campbell K, Magnuson M, Wright C, Kawaguchi Y, Ikenaka K, Takebayashi H, Ishiwata S, Ono Y, Hoshino M Temporal identity transition from Purkinje cell progenitors to GABAergic interneuron progenitors in the cerebellum. Nat Commun, 2014;5(0):3337. 2014 [PMID: 24535035] (IHC, Mouse)
• Martinez-Cerdeno V, Lemen JM, Chan V, Wey A, Lin W, Dent SR, Knoepfler PS N-Myc and GCN5 regulate significantly overlapping transcriptional programs in neural stem cells. PLoS ONE, 2012;7(6):e39456. 2012 [PMID: 22745758] (IHC-P, Mouse)
• Vue TY, Aaker J, Taniguchi A Characterization of progenitor domains in the developing mouse thalamus. J. Comp. Neurol., 2007;505(1):73-91. 2007 [PMID: 17729296] (IHC-Fr, Mouse)
• Srikanth , Maya, Kim , Juno, Das , Sunit, Kessler , John A BMP signaling induces astrocytic differentiation of clinically derived oligodendroglioma propagating cells. Mol Cancer Res, 2014;12(2):283-94. 2014 [PMID: 24269952] (IHC-Fr, Human)
• Piltti K, Salazar D, Uchida N, Cummings B, Anderson A Safety of epicenter versus intact parenchyma as a transplantation site for human neural stem cells for spinal cord injury therapy. Stem Cells Transl Med, 2013;2(3):204-16. 2013 [PMID: 23413374] (ICC, Human)
• Kuboyama , Kazuya, Fujikawa , Akihiro, Masumura , Makoto, Suzuki , Ryoko, Matsumoto , Masahito, Noda , Masaharu Protein tyrosine phosphatase receptor type z negatively regulates oligodendrocyte differentiation and myelination. PLoS ONE, 2012;7(11):e48797. 2012 [PMID: 23144976] (ICC, Mouse)
• Zelinka C, Scott M, Volkov L, Fischer A The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1. PLoS ONE, 2012;7(9):e44477. 2012 [PMID: 22973454] (IHC, Chicken)
• Salazar DL, Uchida N, Hamers FP, Cummings BJ, Anderson AJ Human neural stem cells differentiate and promote locomotor recovery in an early chronic spinal cord injury NOD-scid mouse model. PLoS ONE, 2010;5(8):e12272. 2010 [PMID: 20806064] (ICC, Human)
• Widestrand A, Faijerson J, Wilhelmsson U, Smith PL, Li L, Sihlbom C, Eriksson PS, Pekny M Increased neurogenesis and astrogenesis from neural progenitor cells grafted in the hippocampus of GFAP-/- Vim-/- mice. Stem Cells, 2007;25(10):2619-27. 2007 [PMID: 17628017] (ICC, Rat)
• Bauer S, Patterson PH Leukemia inhibitory factor promotes neural stem cell self-renewal in the adult brain. J. Neurosci., 2006;26(46):12089-99. 2006 [PMID: 17108182] (IHC-Fr, Mouse)

Reviews for Olig2 Antibody (AF2418) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Xuecheng Qiu IHC-Fr Mouse 05/12/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Mouse brain
• Species: Mouse
• Lot: UPA0617051

reviewed by: Verified Customer ICC Mouse 01/06/2015

Summary

• Application: Immunocytochemistry
• Sample Tested: See PMID 22504067
• Species: Mouse

Blocking

• Blocking Details: See PMID 22504067

Details

• Detection Notes: See PMID 22504067

Comments

• Comments: Also used immunohistochemistry frozenPublished. See PMID 22504067

Bioinformatics

• Gene Symbol: OLIG2
• Uniprot:
  • Q13516()