Olig2 Antibody [Unconjugated]

Images

 
A172 human glioblastoma cell line were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Olig2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse/Rat Olig2 ...read more
Olig2 and Oligodendrocyte Marker O4 were detected in 7 day differentiated rat cortical stem cells using 10 µg/mL Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) and 10 ...read more
Olig2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) at 8 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Olig2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Olig2
Met1-Lys323
Accession # Q13516
Specificity
Detects human Olig2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
OLIG2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF2418 in the following applications:

Publications
Read Publications using
AF2418 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Olig2 Antibody [Unconjugated]

  • BHLHB1
  • BHLHE19
  • Class B basic helix-loop-helix protein 1
  • Class E basic helix-loop-helix protein 19
  • human protein kinase C-binding protein RACK17
  • Olig2
  • OLIGO2
  • oligodendrocyte lineage transcription factor 2
  • oligodendrocyte transcription factor 2
  • oligodendrocyte-specific bHLH transcription factor 2
  • PRKCBP2
  • protein kinase C binding protein 2
  • Protein kinase C-binding protein 2
  • Protein kinase C-binding protein RACK17
  • RACK17
  • RACK17helix-loop-helix protein, class B, 1

Background

Olig1 and Olig2 are basic-helix-loop-helix (bHLH) transcription factors expressed in the motor neuron progenitor (pMN) domain of the spinal cord that generates motor neurons and oligodendrocytes. Olig1 is involved in the development and maturation of oligodendrocytes. Olig2 is required for oligodendrocyte and motor neuron specification in the spinal cord.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Olig2 Antibody (AF2418)(68)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 9 applications: ChIP, Co-Immunoprecipitation, FISH, ICC, IHC, IHC-Fr, IHC-P, NotWestern Blot, Western Blot.


Filter By Application
ChIP
(1)
Co-Immunoprecipitation
(1)
FISH
(1)
ICC
(15)
IHC
(33)
IHC-Fr
(7)
IHC-P
(2)
NotWestern Blot
(1)
Western Blot
(6)
All Applications
Filter By Species
Human
(23)
Mouse
(31)
Rat
(11)
Chicken
(2)
All Species
Showing Publications 1 - 10 of 68. Show All 68 Publications.
Publications using AF2418 Applications Species
C Azevedo, G Teku, Y Pomeshchik, JF Reyes, M Chumarina, K Russ, E Savchenko, A Hammarberg, NJ Lamas, A Collin, GK Gouras, O Klementiev, M Hallbeck, R Taipa, M Vihinen, L Roybon Parkinson's disease and multiple system atrophy patient iPSC-derived oligodendrocytes exhibit alpha-synuclein-induced changes in maturation and immune reactive properties Proceedings of the National Academy of Sciences of the United States of America, 2022;119(12):e2111405119. 2022 [PMID: 35294277] (ICC, Human) ICC Human
T Feng, L Luan, II Katz, M Ullah, VM Van Deerli, JQ Trojanowsk, EB Lee, F Hu TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss Acta neuropathologica communications, 2022;10(1):33. 2022 [PMID: 35287730] (Western Blot, Mouse) Western Blot Mouse
WU Park, GB Yeon, MS Yu, HG Goo, SH Hwang, D Na, DS Kim A Novel Vitronectin Peptide Facilitates Differentiation of Oligodendrocytes from Human Pluripotent Stem Cells (Synthetic ECM for Oligodendrocyte Differentiation) Biology, 2021;10(12):. 2021 [PMID: 34943169] (ICC, Human) ICC Human
LM Toomey, M Papini, B Lins, AJ Wright, A Warnock, T McGonigle, SC Hellewell, CA Bartlett, C Anyaegbu, M Fitzgerald Cuprizone feed formulation influences the extent of demyelinating disease pathology Scientific Reports, 2021;11(1):22594. 2021 [PMID: 34799634] (IHC, Mouse) IHC Mouse
Y Kimura, Y Hayashi, S Hitomi, D Ikutame, K Urata, I Shibuta, A Sakai, J Ni, K Iwata, M Tonogi, M Shinoda IL-33 induces orofacial neuropathic pain through Fyn-dependent phosphorylation of GluN2B in the trigeminal spinal nucleus caudalis Brain, Behavior, and Immunity, 2021;0(0):. 2021 [PMID: 34715301] (IHC, Mouse) IHC Mouse
Y Takanezawa, S Tanabe, D Kato, R Ozeki, M Komoda, T Suzuki, H Baba, R Muramatsu Microglial ASD-related genes are involved in oligodendrocyte differentiation Scientific Reports, 2021;11(1):17825. 2021 [PMID: 34497307] (IHC, Mouse) IHC Mouse
LE Blizzard, C Menke, SD Patel, RR Waclaw, SA Lachke, RW Stottmann A Novel Mutation in Cse1l Disrupts Brain and Eye Development with Specific Effects on Pax6 Expression Journal of developmental biology, 2021;9(3):. 2021 [PMID: 34287339] (IHC, Mouse) IHC Mouse
M Connor, K Lamorie-Fo, Q Liu, K Shkirkova, H Baertsch, C Sioutas, TE Morgan, CE Finch, WJ Mack Nanoparticulate matter exposure results in white matter damage and an inflammatory microglial response in an experimental murine model PLoS ONE, 2021;16(7):e0253766. 2021 [PMID: 34214084] (IHC, Mouse) IHC Mouse
T Zhang, T Liu, N Mora, J Guegan, M Bertrand, X Contreras, AH Hansen, C Streicher, M Anderle, N Danda, L Tiberi, S Hippenmeye, BA Hassan Generation of excitatory and inhibitory neurons from common progenitors via Notch signaling in the cerebellum Cell Reports, 2021;35(10):109208. 2021 [PMID: 34107249] (IHC, Mouse) IHC Mouse
SM Nam, JH Choi, SH Choi, HJ Cho, YJ Cho, H Rhim, HC Kim, IH Cho, DG Kim, SY Nah Ginseng gintonin alleviates neurological symptoms in the G93A-SOD1 transgenic mouse model of amyotrophic lateral sclerosis through lysophosphatidic acid 1 receptor Journal of Ginseng Research, 2021;45(3):390-400. 2021 [PMID: 34025132] (IHC, Mouse) IHC Mouse
Show All 68 Publications.

Reviews for Olig2 Antibody (AF2418) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF2418:
Filter by Applications
IHC-Fr
(1)
ICC
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Olig2 AF2418
Enlarge
5
reviewed by:
Xuecheng Qiu
IHC-Fr Mouse 05/12/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Tested Mouse brain
SpeciesMouse
LotUPA0617051
  4
reviewed by:
Verified Customer
ICC Mouse 01/06/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 22504067
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22504067

Details

Detection NotesSee PMID 22504067

Comments

CommentsAlso used immunohistochemistry frozenPublished. See PMID 22504067

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Xuecheng Qiu
05/12/2019
Application: IHC-Fr
Species: Mouse

 
Verified Customer
01/06/2015
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol OLIG2
Uniprot