Olig2 Antibody [Unconjugated]

Images

 
A172 human glioblastoma cell line were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Olig2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse/Rat Olig2 ...read more
Olig2 and Oligodendrocyte Marker O4 were detected in 7 day differentiated rat cortical stem cells using 10 µg/mL Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) and 10 ...read more
Olig2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) at 8 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Olig2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Olig2
Met1-Lys323
Accession # Q13516
Specificity
Detects human Olig2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Olig3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
OLIG2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF2418 in the following applications:

Publications
Read Publications using
AF2418 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Olig2 Antibody [Unconjugated]

  • BHLHB1
  • BHLHE19
  • Class B basic helix-loop-helix protein 1
  • Class E basic helix-loop-helix protein 19
  • human protein kinase C-binding protein RACK17
  • Olig2
  • Oligo2
  • oligodendrocyte lineage transcription factor 2
  • oligodendrocyte transcription factor 2
  • oligodendrocyte-specific bHLH transcription factor 2
  • PRKCBP2
  • protein kinase C binding protein 2
  • Protein kinase C-binding protein 2
  • Protein kinase C-binding protein RACK17
  • RACK17
  • RACK17helix-loop-helix protein, class B, 1

Background

Olig1 and Olig2 are basic-helix-loop-helix (bHLH) transcription factors expressed in the motor neuron progenitor (pMN) domain of the spinal cord that generates motor neurons and oligodendrocytes. Olig1 is involved in the development and maturation of oligodendrocytes. Olig2 is required for oligodendrocyte and motor neuron specification in the spinal cord.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Olig2 Antibody (AF2418)(29)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 14 applications: ChIP Assay, ICC, ICC Fluorescence, IF, IHC, IHC Frozen, IHC - PFA fixed, IHC Frozen, IHC Not Specified, IHC OCT, IHC OCT-embedded, IHC Paraffin-embedded, NotWB, WB.


Filter By Application
ChIP Assay
(1)
ICC
(6)
ICC Fluorescence
(2)
IF
(1)
IHC
(4)
IHC Frozen
(1)
IHC - PFA fixed
(1)
IHC Frozen
(2)
IHC Not Specified
(2)
IHC OCT
(1)
IHC OCT-embedded
(1)
IHC Paraffin-embedded
(2)
NotWB
(1)
WB
(3)
All Applications
Filter By Species
Human
(13)
Mouse
(10)
Rat
(4)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using AF2418 Applications Species
T Srivastava, P Diba, JM Dean, F Banine, D Shaver, M Hagen, X Gong, W Su, B Emery, DL Marks, EN Harris, B Baggenstos, PH Weigel, LS Sherman, SA Back A TLR/AKT/FoxO3 immune tolerance-like pathway disrupts the repair capacity of oligodendrocyte progenitors J. Clin. Invest., 2018;0(0):. 2018 [PMID: 29664021] (IHC,, Rat) IHC Rat
W Zhou, C Chen, Y Shi, Q Wu, RC Gimple, X Fang, Z Huang, K Zhai, SQ Ke, YF Ping, H Feng, JN Rich, JS Yu, S Bao, XW Bian Targeting Glioma Stem Cell-Derived Pericytes Disrupts the Blood-Tumor Barrier and Improves Chemotherapeutic Efficacy Cell Stem Cell, 2017;21(5):591-603.e4. 2017 [PMID: 29100012] (IHC,, Human) IHC Human
FI Young, M Keruzore, X Nan, N Gennet, EJ Bellefroid, M Li The doublesex-related Dmrta2 safeguards neural progenitor maintenance involving transcriptional regulation of Hes1 Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28655839] (ICC,, Mouse) ICC Mouse
NJ Yates, MK Giacci, RL O'Hare Doi, W Chiha, BE Ashworth, J Kenna, CA Bartlett, M Fitzgerald Delayed treatment of secondary degeneration following acute optic nerve transection using a combination of ion channel inhibitors Neural Regen Res, 2017;12(2):307-316. 2017 [PMID: 28400815] (IHC OCT-embedded,, Rat) IHC OCT-embedded Rat
Brain tissue banking for stem cells for our future Sci Rep, 2016;6(0):39394. 2016 [PMID: 27991551] (IHC Not Specified,, Human) IHC Not Specified Human
Majistor Raj Luxman Maglo Renkilaraj The intellectual disability protein PAK3 regulates oligodendrocyte precursor cell differentiation Neurobiol. Dis, 2016;0(0):. 2016 [PMID: 27940202] (ICC,, Mouse) ICC Mouse
A cord blood monocyte-derived cell therapy product accelerates brain remyelination JCI Insight, 2016;1(13):e86667. 2016 [PMID: 27699230] (IHC Not Specified,, Mouse) IHC Not Specified Mouse
Patched1 and Patched2 inhibit Smoothened non-cell autonomously Elife, 2016;5(0):. 2016 [PMID: 27552050] (ICC Fluorescence,, Mouse) ICC Fluorescence Mouse
Qi Xie RBPJ maintains brain tumor-initiating cells through CDK9-mediated transcriptional elongation J Clin Invest, 2016;126(7):2757-2772. 2016 [PMID: 27322055] (WB,, Human) WB Human
Show All 29 Publications.

Review for Olig2 Antibody (AF2418) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF2418:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ICC Mouse 01/06/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 22504067
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22504067

Details

Detection NotesSee PMID 22504067

Comments

CommentsAlso used immunohistochemistry frozenPublished. See PMID 22504067

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Recent Reviews

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1
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Anonymous
01/06/2015
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol OLIG2
Entrez
Uniprot