Olig2 Antibody [Unconjugated]

Images

 
A172 human glioblastoma cell line were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Olig2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse/Rat Olig2 ...read more
Olig2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) at 8 µg/mL overnight at 4 °C. ...read more
Olig2 and Oligodendrocyte Marker O4 were detected in 7 day differentiated rat cortical stem cells using 10 µg/mL Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) and 10 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Olig2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Olig2
Met1-Lys323
Accession # Q13516
Specificity
Detects human Olig2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Olig3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
OLIG2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF2418 in the following applications:

Publications
Read Publications using
AF2418 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Olig2 Antibody [Unconjugated]

  • BHLHB1
  • BHLHE19
  • Class B basic helix-loop-helix protein 1
  • Class E basic helix-loop-helix protein 19
  • human protein kinase C-binding protein RACK17
  • Olig2
  • OLIGO2
  • oligodendrocyte lineage transcription factor 2
  • oligodendrocyte transcription factor 2
  • oligodendrocyte-specific bHLH transcription factor 2
  • PRKCBP2
  • protein kinase C binding protein 2
  • Protein kinase C-binding protein 2
  • Protein kinase C-binding protein RACK17
  • RACK17
  • RACK17helix-loop-helix protein, class B, 1

Background

Olig1 and Olig2 are basic-helix-loop-helix (bHLH) transcription factors expressed in the motor neuron progenitor (pMN) domain of the spinal cord that generates motor neurons and oligodendrocytes. Olig1 is involved in the development and maturation of oligodendrocytes. Olig2 is required for oligodendrocyte and motor neuron specification in the spinal cord.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Olig2 Antibody (AF2418)(43)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 7 applications: ChIP, ICC, IHC, IHC-Fr, IHC-P, NotWestern Blot, Western Blot.


Filter By Application
ChIP
(1)
ICC
(9)
IHC
(19)
IHC-Fr
(7)
IHC-P
(2)
NotWestern Blot
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(15)
Mouse
(18)
Rat
(8)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 43. Show All 43 Publications.
Publications using AF2418 Applications Species
C Ogawa, S Mikawa, K Yamashita, Y Hayashi, K Masumoto, F Katou, K Sato Follistatin expression in the central nervous system of the adult rat J. Chem. Neuroanat., 2020;105(0):101753. 2020 [PMID: 32014555] (IHC, Rat) IHC Rat
Y Zhou, WM Song, PS Andhey, A Swain, T Levy, KR Miller, PL Poliani, M Cominelli, S Grover, S Gilfillan, M Cella, TK Ulland, K Zaitsev, A Miyashita, T Ikeuchi, M Sainouchi, A Kakita, DA Bennett, JA Schneider, MR Nichols, SA Beausoleil, JD Ulrich, DM Holtzman, MN Artyomov, M Colonna Human and mouse single-nucleus transcriptomics reveal TREM2-dependent and TREM2-independent cellular responses in Alzheimer's disease Nat. Med., 2020;26(1):131-142. 2020 [PMID: 31932797] (IHC, Mouse) IHC Mouse
LM Toomey, CA Bartlett, N Gavriel, T McGonigle, M Majimbi, G Gopalasing, J Rodger, M Fitzgerald Comparing modes of delivery of a combination of ion channel inhibitors for limiting secondary degeneration following partial optic nerve transection Sci Rep, 2019;9(1):15297. 2019 [PMID: 31653948] (IHC, Rat) IHC Rat
S Clavreul, L Abdeladim, E Hernández-, D Niculescu, J Durand, SH Ieng, R Barry, G Bonvento, E Beaurepair, J Livet, K Loulier Cortical astrocytes develop in a plastic manner at both clonal and cellular levels Nat Commun, 2019;10(1):4884. 2019 [PMID: 31653848] (IHC, Mouse) IHC Mouse
M Boström, Y Eriksson, J Danial, T Björk-Erik, M Kalm Chronic disturbance in the thalamus following cranial irradiation to the developing mouse brain Sci Rep, 2019;9(1):9588. 2019 [PMID: 31270437] (IHC, Mouse) IHC Mouse
Y Hayashi, S Mikawa, C Ogawa, K Masumoto, F Katou, K Sato BMP6 expression in the adult rat central nervous system J. Chem. Neuroanat., 2019;0(0):. 2019 [PMID: 30928603] (IHC-Fr, Rat) IHC-Fr Rat
V Bellver-La, F Bretheau, B Mailhot, N Vallières, M Lessard, ME Janelle, N Vernoux, MÈ Tremblay, T Fuehrmann, MS Shoichet, S Lacroix Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury Nat Commun, 2019;10(1):518. 2019 [PMID: 30705270] (IHC, Mouse) IHC Mouse
M Osipovitch, A Asenjo Mar, JN Mariani, A Cornwell, S Dhaliwal, L Zou, D Chandler-M, S Wang, X Li, SJ Benraiss, R Agate, A Lampp, A Benraiss, MS Windrem, SA Goldman Human ESC-Derived Chimeric Mouse Models of Huntington's Disease Reveal Cell-Intrinsic Defects in Glial Progenitor Cell Differentiation Cell Stem Cell, 2018;0(0):. 2018 [PMID: 30554964] (IHC, Human) IHC Human
AM Falcão, D van Brugge, S Marques, M Meijer, S Jäkel, E Agirre, Samudyata, EM Floriddia, DP Vanichkina, C Ffrench-Co, A Williams, AO Guerreiro-, G Castelo-Br Disease-specific oligodendrocyte lineage cells arise in multiple sclerosis Nat. Med., 2018;0(0):. 2018 [PMID: 30420755] (IHC-Fr, Mouse) IHC-Fr Mouse
LM Ruiz-Perer, L Schneider, BA Windmöller, J Müller, JFW Greiner, C Kaltschmid, B Kaltschmid NF-?B p65 directs sex-specific neuroprotection in human neurons Sci Rep, 2018;8(1):16012. 2018 [PMID: 30375448] (ICC, Human) ICC Human
Show All 43 Publications.

Reviews for Olig2 Antibody (AF2418) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF2418:
Filter by Applications
IHC-Fr
(1)
ICC
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Olig2 AF2418
Enlarge
5
reviewed by:
Xuecheng Qiu
IHC-Fr Mouse 05/12/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Tested Mouse brain
SpeciesMouse
LotUPA0617051
  4
reviewed by:
Anonymous
ICC Mouse 01/06/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 22504067
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22504067

Details

Detection NotesSee PMID 22504067

Comments

CommentsAlso used immunohistochemistry frozenPublished. See PMID 22504067

Product General Protocols

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Video Protocols

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FAQs for Olig2 Antibody (AF2418) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF2418

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Xuecheng Qiu
05/12/2019
Application: IHC-Fr
Species: Mouse

 
Anonymous
01/06/2015
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol OLIG2
Uniprot