Neurocan Antibody

Images

 
Western blot shows lysates of rat embryonic hippocampal neurons. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Neurocan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5800) followed by HRP-conjugated ...read more
Neurocan was detected in perfusion fixed frozen sections of mouse brain (medulla) using Mouse/Rat Neurocan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5800) at 15 µg/mL overnight at ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Neurocan Antibody Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Neurocan
Asp23-Asp637
Accession # NP_031815
Specificity
Detects mouse and rat Neurocan in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
Ncan
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF5800 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neurocan Antibody

  • chondroitin sulfate proteoglycan 3 (neurocan)
  • Chondroitin sulfate proteoglycan 3
  • CSPG3
  • CSPG3neurocan core protein
  • FLJ44681
  • NCAN
  • NEUR
  • neurocan proteoglycan
  • Neurocan

Background

Neurocan is a 220 kDa nervous tissue-specific chondroitin sulfate proteoglycan member of the aggrecan/versican proteoglycan family (1). Mouse Neurocan is synthesized as a 1268 amino acid (aa) precursor that contains a 22 aa signal sequence and a 1246 aa mature chain. The mature chain contains one Ig-like V-type domain (aa 37-157), two Link domains (aa 159-254 and 258-356), two EGF-like domains (aa 960-996 and 998-1034), one C-type lectin-like domain (aa 1036-1165), one Sushi domain (aa 1165-1224), and five potential sites for N-linked glycosylation. Mature mouse Neurocan is 90% and 66% aa identical to mature rat and human Neurocan, respectively. Neurocan binds with high affinity to the cell adhesion molecules (CAM) Ng-CAM and N-CAM to inhibit Neuronal adhesion and neurite growth (2-3). In the developing rat retina, the expression of Neurocan is regulated both temporally and spatially, which suggests that it may play a role in the differentiation of and neural network formation of the mammalian retina (1). Injury to the CNS leads to permanent loss of function due to the inability of severed nerve fibers to regenerate back to their targets (4). The lack of CNS repair is attributed in part to the extracellular matrix chondroitin sulfate proteoglycans, such as Neurocan, which are produced by activated glial cells post-injury (4).
  1. Inatani, M. et al. (1999) Invest. Ophthalmol. Vis. Sci. 40:2350.
  2. Friedlander, D.R. et al. (1994) J. Cell Biol. 125:669.
  3. Retzler, C. et al. (1996) J. Biol. Chem. 271:27304.
  4. Quaglia, X. et al. (2008) Brain 131:240.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neurocan Antibody (AF5800)(6)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: IHC Not Specified, IHC- Frozen, IHC-Fr, WB.


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IHC Not Specified
(1)
IHC- Frozen
(1)
IHC-Fr
(1)
WB
(3)
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Filter By Species
Mouse
(6)
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Showing Publications 1 - 6 of 6.
Publications using AF5800 Applications Species
J Chen, S Van Gulden, TL McGuire, AC Fleming, C Oka, JA Kessler, CY Peng BMP-responsive Protease HtrA1 is Differentially Expressed in Astrocytes and Regulates Astrocytic Development and Injury Response J. Neurosci., 2018;0(0):. 2018 [PMID: 29483282] (WB,, Mouse) WB Mouse
H Ueno, S Suemitsu, M Okamoto, Y Matsumoto, T Ishihara Sensory experience-dependent formation of perineuronal nets and expression of Cat-315 immunoreactive components in the mouse somatosensory cortex Neuroscience, 2017;0(0):. 2017 [PMID: 28495333] (IHC-Fr,, Mouse) IHC-Fr Mouse
Protein O-Mannosylation in the Murine Brain: Occurrence of Mono-O-Mannosyl Glycans and Identification of New Substrates PLoS ONE, 2016;11(11):e0166119. 2016 [PMID: 27812179] (IHC- Frozen,, Mouse) IHC- Frozen Mouse
Ebbers L, Satheesh S, Janz K, Ruttiger L, Blosa M, Hofmann F, Morawski M, Griesemer D, Knipper M, Friauf E, Nothwang H L-type Calcium Channel Cav1.2 Is Required for Maintenance of Auditory Brainstem Nuclei. J Biol Chem, 2015;290(39):23692-710. 2015 [PMID: 26242732] (IHC Not Specified,, Mouse) IHC Not Specified Mouse
Dwyer C, Katoh T, Tiemeyer M, Matthews R Neurons and glia modify receptor protein-tyrosine phosphatase zeta (RPTPzeta)/phosphacan with cell-specific O-mannosyl glycans in the developing brain. J Biol Chem, 2015;290(16):10256-73. 2015 [PMID: 25737452] (WB,, Mouse) WB Mouse
Bekku Y, Oohashi T, Neurocan contributes to the molecular heterogeneity of the perinodal ECM. Arch. Histol. Cytol., 2010;73(2):95-102. 2010 [PMID: 21566336] (WB,, Mouse) WB Mouse

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Bioinformatics

Gene Symbol Ncan
Entrez
Uniprot