Neurocan Antibody

Western blot shows lysates of rat embryonic hippocampal neurons. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Neurocan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5800) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neurocan at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Neurocan was detected in perfusion fixed frozen sections of mouse brain (medulla) using Mouse/Rat Neurocan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5800) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Neurocan Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Neurocan
  Asp23-Asp637
  Accession # NP_031815
• Specificity: Detects mouse and rat Neurocan in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: Ncan
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neurocan Antibody

• chondroitin sulfate proteoglycan 3 (neurocan)
• Chondroitin sulfate proteoglycan 3
• CSPG3
• CSPG3neurocan core protein
• FLJ44681
• NCAN
• NEUR
• neurocan proteoglycan
• Neurocan

Background

Neurocan is a 220 kDa nervous tissue-specific chondroitin sulfate proteoglycan member of the aggrecan/versican proteoglycan family (1). Mouse Neurocan is synthesized as a 1268 amino acid (aa) precursor that contains a 22 aa signal sequence and a 1246 aa mature chain. The mature chain contains one Ig-like V-type domain (aa 37-157), two Link domains (aa 159-254 and 258-356), two EGF-like domains (aa 960-996 and 998-1034), one C-type lectin-like domain (aa 1036-1165), one Sushi domain (aa 1165-1224), and five potential sites for N-linked glycosylation. Mature mouse Neurocan is 90% and 66% aa identical to mature rat and human Neurocan, respectively. Neurocan binds with high affinity to the cell adhesion molecules (CAM) Ng-CAM and N-CAM to inhibit Neuronal adhesion and neurite growth (2-3). In the developing rat retina, the expression of Neurocan is regulated both temporally and spatially, which suggests that it may play a role in the differentiation of and neural network formation of the mammalian retina (1). Injury to the CNS leads to permanent loss of function due to the inability of severed nerve fibers to regenerate back to their targets (4). The lack of CNS repair is attributed in part to the extracellular matrix chondroitin sulfate proteoglycans, such as Neurocan, which are produced by activated glial cells post-injury (4).

1. Inatani, M. et al. (1999) Invest. Ophthalmol. Vis. Sci. 40:2350.
2. Friedlander, D.R. et al. (1994) J. Cell Biol. 125:669.
3. Retzler, C. et al. (1996) J. Biol. Chem. 271:27304.
4. Quaglia, X. et al. (2008) Brain 131:240.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neurocan Antibody (AF5800)(12)

Publications using AF5800

• D Irala, S Wang, K Sakers, L Nagendren, FP Ulloa-Seve, DS Bindu, C Eroglu Astrocyte-Secreted Neurocan Controls Inhibitory Synapse Formation and Function bioRxiv : the preprint server for biology, 2023-04-03;0(0):. 2023-04-03 [PMID: 37066164] (IHC, Western Blot, Mouse)
• A Ritok, P Kiss, A Zaher, E Wolf, L Ducza, T Bacskai, C Matesz, B Gaal Distribution and postnatal development of chondroitin sulfate proteoglycans in the perineuronal nets of cholinergic motoneurons innervating extraocular muscles Scientific Reports, 2022-12-14;12(1):21606. 2022-12-14 [PMID: 36517521] (IHC, Mouse)
• W Hu beta ler, L Höhn, C Stolz, S Vielhaber, C Garz, FC Schmitt, ED Gundelfing, S Schreiber, CI Seidenbech Brevican and Neurocan Cleavage Products in the Cerebrospinal Fluid - Differential Occurrence in ALS, Epilepsy and Small Vessel Disease Frontiers in Cellular Neuroscience, 2022-04-11;16(0):838432. 2022-04-11 [PMID: 35480959] (ELISA Development, Human)
• T Fontanil, Y Mohamedi, A Moncada-Pa, T Cobo, JA Vega, JL Cobo, O García-Suá, J Cobo, ÁJ Obaya, S Cal Neurocan is a New Substrate for the ADAMTS12 Metalloprotease: Potential Implications in Neuropathies Cell. Physiol. Biochem., 2019-01-01;52(5):1003-1016. 2019-01-01 [PMID: 30977985] (IHC-P, Western Blot, Mouse)
• H Ueno, S Suemitsu, S Murakami, N Kitamura, K Wani, Y Matsumoto, M Okamoto, T Ishihara Layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices IBRO Rep, 2018-11-28;6(0):1-17. 2018-11-28 [PMID: 30582064] (IHC-Fr, Mouse)
• M Sonntag, M Blosa, S Schmidt, K Reimann, K Blum, T Eckrich, G Seeger, D Hecker, B Schick, T Arendt, J Engel, M Morawski Synaptic coupling of inner ear sensory cells is controlled by brevican-based extracellular matrix baskets resembling perineuronal nets BMC Biol., 2018-09-26;16(1):99. 2018-09-26 [PMID: 30253762] (IHC, Western Blot, Mouse)
• J Chen, S Van Gulden, TL McGuire, AC Fleming, C Oka, JA Kessler, CY Peng BMP-responsive Protease HtrA1 is Differentially Expressed in Astrocytes and Regulates Astrocytic Development and Injury Response J. Neurosci., 2018-02-26;0(0):. 2018-02-26 [PMID: 29483282] (Western Blot, Mouse)
• H Ueno, S Suemitsu, M Okamoto, Y Matsumoto, T Ishihara Sensory experience-dependent formation of perineuronal nets and expression of Cat-315 immunoreactive components in the mouse somatosensory cortex Neuroscience, 2017-05-08;0(0):. 2017-05-08 [PMID: 28495333] (IHC-Fr, Mouse)
• Protein O-Mannosylation in the Murine Brain: Occurrence of Mono-O-Mannosyl Glycans and Identification of New Substrates PLoS ONE, 2016-11-03;11(11):e0166119. 2016-11-03 [PMID: 27812179] (IHC-Fr, Mouse)
• Ebbers L, Satheesh S, Janz K, Ruttiger L, Blosa M, Hofmann F, Morawski M, Griesemer D, Knipper M, Friauf E, Nothwang H L-type Calcium Channel Cav1.2 Is Required for Maintenance of Auditory Brainstem Nuclei. J Biol Chem, 2015-08-04;290(39):23692-710. 2015-08-04 [PMID: 26242732] (IHC, Mouse)
• Dwyer C, Katoh T, Tiemeyer M, Matthews R Neurons and glia modify receptor protein-tyrosine phosphatase zeta (RPTPzeta)/phosphacan with cell-specific O-mannosyl glycans in the developing brain. J Biol Chem, 2015-03-03;290(16):10256-73. 2015-03-03 [PMID: 25737452] (Western Blot, Mouse)
• Bekku Y, Oohashi T Neurocan contributes to the molecular heterogeneity of the perinodal ECM. Arch. Histol. Cytol., 2010-01-01;73(2):95-102. 2010-01-01 [PMID: 21566336] (IHC-Fr, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: Ncan
• Uniprot:
  • NP_031815()