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NG2/MCSP Antibody (LHM-2) [Alexa Fluor® 488]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
LHM-2
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB2585U-100UG
Alexa Fluor 405
FAB2585V-100UG
Alexa Fluor 594
FAB2585T-100UG
Alexa Fluor 647
FAB2585R-100UG
Alexa Fluor 700
FAB2585N-100UG
Alexa Fluor 750
FAB2585S-100UG
Allophycocyanin
FAB2585A
Fluorescein
FAB2585F
PerCP (Peridinin-chlorophyll Protein Complex)
FAB2585C
Phycoerythrin
FAB2585P
Unconjugated
FAB2585C
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Catalog# & Formulation Size Price
Standard Formulation
FAB2585C
Carrier Free
FAB2585A
Carrier Free
FAB2585C
Carrier Free
FAB2585F
Carrier Free
FAB2585P
Carrier Free
FAB2585V-100UG
Carrier Free
FAB2585U-100UG
Carrier Free
FAB2585T-100UG
Carrier Free
FAB2585S-100UG
Carrier Free
FAB2585R-100UG
Carrier Free
FAB2585N-100UG
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NG2/MCSP Antibody (LHM-2) [Alexa Fluor® 488] Summary

Immunogen
Human melanoma cells
Specificity
Detects human NG2/MCSP in Western blots.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CSPG4
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NG2/MCSP Antibody (LHM-2) [Alexa Fluor® 488]

  • AN2
  • chondroitin sulfate proteoglycan 4 (melanoma-associated)
  • chondroitin sulfate proteoglycan 4
  • CSPG4
  • EC 3.6.3
  • HMW-MAA
  • MCSP
  • MCSPChondroitin sulfate proteoglycan NG2
  • MCSPG
  • MCSPGMelanoma chondroitin sulfate proteoglycan
  • MEL-CSPG
  • MSK16Melanoma-associated chondroitin sulfate proteoglycan
  • NG2
  • NG2EC 2.7.8

Background

NG2, also known as MCSP, is a chondroitin sulfate proteoglycan that has been used as a cell surface marker for melanoma (1) and glial precursor cells (2). NG2 also promotes epidermal stem cell patterned clustering (3).
  1. Kupsch, J.M. et al. (1995) Melanoma Res. 5:403.
  2. Stegmuller, J. et al. (2002) J. Neurocytol. 31:497.
  3. Legg, J. et al. (2003) Development 130:6049.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NOD2 Antibody (2D9)
NB100-524
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Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB2585U
Alexa Fluor 405 FAB2585V
Alexa Fluor 488 FAB2585G
Alexa Fluor 594 FAB2585T
Alexa Fluor 647 FAB2585R
Alexa Fluor 700 FAB2585N
Alexa Fluor 750 FAB2585S
Allophycocyanin FAB2585A
Fluorescein FAB2585F
PerCP (Peridinin-chlorophyll Protein Complex) FAB2585C
Phycoerythrin FAB2585P
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Bioinformatics

Gene Symbol CSPG4