NG2/MCSP Antibody (LHM-2) [Fluorescein]

A375 cells were stained with Mouse Anti-Human NG2/MCSP Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB2585F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

G361 human melanoma cell line was stained with Mouse Anti-Human NG2/MCSP Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2585F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: LHM-2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

NG2/MCSP Antibody (LHM-2) [Fluorescein] Summary

• Immunogen: Human melanoma cells
• Specificity: Detects human NG2/MCSP in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSPG4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NG2/MCSP Antibody (LHM-2) [Fluorescein]

• AN2
• chondroitin sulfate proteoglycan 4 (melanoma-associated)
• chondroitin sulfate proteoglycan 4
• CSPG4
• EC 3.6.3
• HMW-MAA
• MCSP
• MCSPChondroitin sulfate proteoglycan NG2
• MCSPG
• MCSPGMelanoma chondroitin sulfate proteoglycan
• MEL-CSPG
• MSK16Melanoma-associated chondroitin sulfate proteoglycan
• NG2
• NG2EC 2.7.8

Background

NG2, also known as MCSP, is a chondroitin sulfate proteoglycan that has been used as a cell surface marker for melanoma (1) and glial precursor cells (2). NG2 also promotes epidermal stem cell patterned clustering (3).

1. Kupsch, J.M. et al. (1995) Melanoma Res. 5:403.
2. Stegmuller, J. et al. (2002) J. Neurocytol. 31:497.
3. Legg, J. et al. (2003) Development 130:6049.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NG2/MCSP Antibody (FAB2585F)(3)

Publications using FAB2585F

• J Montero, C Gstalder, DJ Kim, D Sadowicz, W Miles, M Manos, JR Cidado, J Paul Secri, AE Tron, K Flaherty, F Stephen Ho, CH Yoon, A Letai, DE Fisher, R Haq Destabilization of NOXA mRNA as a common resistance mechanism to targeted therapies Nat Commun, 2019-11-14;10(1):5157. 2019-11-14 [PMID: 31727958] (FACS, Human)
• Huang L, Nakayama H, Klagsbrun M, Mulliken J, Bischoff J Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells. Stem Cells, 2015-01-01;33(1):133-45. 2015-01-01 [PMID: 25187207] (Flow Cytometry, Human)
• Guijarro-Munoz I, Cuesta AM, Alvarez-Cienfuegos A, Geng JG, Alvarez-Vallina L, Sanz L The axonal repellent Slit2 inhibits pericyte migration: potential implications in angiogenesis. Exp. Cell Res., 2011-12-14;318(4):371-8. 2011-12-14 [PMID: 22198087] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CSPG4