NG2/MCSP Antibody (LHM-2) [Allophycocyanin]

A375 cells were stained with Mouse Anti-Human NG2/MCSP APC‑conjugated Monoclonal Antibody (Catalog # FAB2585A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

G361 human melanoma cell line was stained with Mouse Anti-Human NG2/MCSP APC-conjugated Monoclonal Antibody (Catalog # FAB2585A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: LHM-2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

NG2/MCSP Antibody (LHM-2) [Allophycocyanin] Summary

• Immunogen: Human melanoma cells
• Specificity: Detects human NG2/MCSP in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSPG4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NG2/MCSP Antibody (LHM-2) [Allophycocyanin]

• AN2
• chondroitin sulfate proteoglycan 4 (melanoma-associated)
• chondroitin sulfate proteoglycan 4
• CSPG4
• EC 3.6.3
• HMW-MAA
• MCSP
• MCSPChondroitin sulfate proteoglycan NG2
• MCSPG
• MCSPGMelanoma chondroitin sulfate proteoglycan
• MEL-CSPG
• MSK16Melanoma-associated chondroitin sulfate proteoglycan
• NG2
• NG2EC 2.7.8

Background

NG2, also known as MCSP, is a chondroitin sulfate proteoglycan that has been used as a cell surface marker for melanoma (1) and glial precursor cells (2). NG2 also promotes epidermal stem cell patterned clustering (3).

1. Kupsch, J.M. et al. (1995) Melanoma Res. 5:403.
2. Stegmuller, J. et al. (2002) J. Neurocytol. 31:497.
3. Legg, J. et al. (2003) Development 130:6049.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NG2/MCSP Antibody (FAB2585A)(8)

Publications using FAB2585A

• Richards, A;Khalil, A;Friesen, M;Whitfield, TW;Lungjangwa, T;Gehrke, L;Mooney, D;Jaenisch, R; SARS-CoV-2 infection of human pluripotent stem cell-derived vascular cells reveals smooth muscle cells as key mediators of vascular pathology during infection bioRxiv : the preprint server for biology 2023-08-07 [PMID: 37609322] (Flow Cytometry, Human)
• Potez M, Snedal S, She C et al. Use of phage display biopanning as a tool to design CAR-T cells against glioma stem cells Frontiers in Oncology 2023-03-24 [PMID: 37035164]
• Haipeng Zhou, Ying He, Zhaoyan Wang, Qian Wang, Caiyan Hu, Xiaohua Wang et al. Identifying the functions of two biomarkers in human oligodendrocyte progenitor cell development Journal of Translational Medicine 2021-12-01 [PMID: 33933125]
• Haipeng Zhou, Siliang Lu, Ke Li, Yinxiang Yang, Caiyan Hu, Zhaoyan Wang et al. Study on the Safety of Human Oligodendrocyte Precursor Cell Transplantation in Young Animals and Its Efficacy on Myelination Stem Cells and Development 2021-06-01 [PMID: 33823616]
• Madsen SD, Jones SH, Tucker HA et al. Survival of aging CD264+ and CD264- populations of human bone marrow mesenchymal stem cells is independent of colony-forming efficiency Biotechnol. Bioeng. 10/15/2019 [PMID: 31612990]
• G MacLeod, DA Bozek, N Rajakulend, V Monteiro, M Ahmadi, Z Steinhart, MM Kushida, H Yu, FJ Coutinho, FMG Cavalli, I Restall, X Hao, T Hart, HA Luchman, S Weiss, PB Dirks, S Angers Genome-Wide CRISPR-Cas9 Screens Expose Genetic Vulnerabilities and Mechanisms of Temozolomide Sensitivity in Glioblastoma Stem Cells Cell Rep, 2019-04-16;27(3):971-986.e9. 2019-04-16 [PMID: 30995489] (Flow Cytometry, Human)
• CL Esteves, TA Sheldrake, L Dawson, T Menghini, E Rink, K Amilon, N Khan, B Peault, X Donadeu Equine Mesenchymal Stromal Cells retain a pericyte-like phenotype Stem Cells Dev., 2017-05-09;0(0):. 2017-05-09 [PMID: 28376684] (Flow Cytometry, Equine)
• Frassanito M, Rao L, Moschetta M, Ria R, di Marzo L, De Luisi A, Racanelli V, Catacchio I, Berardi S, Basile A, Menu E, Ruggieri S, Nico B, Ribatti D, Fumarulo R, Dammacco F, Vanderkerken K, Vacca A Bone marrow fibroblasts parallel multiple myeloma progression in patients and mice: in vitro and in vivo studies. 2013-09-02;0(0):. 2013-09-02 [PMID: 23995611] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CSPG4