NG2/MCSP Antibody (LHM-2) [PerCP (Peridinin-chlorophyll Protein Complex)]

A375 cells were stained with Mouse Anti-Human NG2/MCSP PerCP‑conjugated Monoclonal Antibody (Catalog # FAB2585C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). View our protocol for Staining Membrane-associated Proteins.

G361 human melanoma cell line was stained with Mouse Anti-Human NG2/MCSP PerCP-conjugated Monoclonal Antibody (Catalog # FAB2585C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: LHM-2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PerCP (Peridinin-chlorophyll Protein Complex)

NG2/MCSP Antibody (LHM-2) [PerCP (Peridinin-chlorophyll Protein Complex)] Summary

• Immunogen: Human melanoma cells
• Specificity: Detects human NG2/MCSP in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSPG4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NG2/MCSP Antibody (LHM-2) [PerCP (Peridinin-chlorophyll Protein Complex)]

• AN2
• chondroitin sulfate proteoglycan 4 (melanoma-associated)
• chondroitin sulfate proteoglycan 4
• CSPG4
• EC 3.6.3
• HMW-MAA
• MCSP
• MCSPChondroitin sulfate proteoglycan NG2
• MCSPG
• MCSPGMelanoma chondroitin sulfate proteoglycan
• MEL-CSPG
• MSK16Melanoma-associated chondroitin sulfate proteoglycan
• NG2
• NG2EC 2.7.8

Background

NG2, also known as MCSP, is a chondroitin sulfate proteoglycan that has been used as a cell surface marker for melanoma (1) and glial precursor cells (2). NG2 also promotes epidermal stem cell patterned clustering (3).

1. Kupsch, J.M. et al. (1995) Melanoma Res. 5:403.
2. Stegmuller, J. et al. (2002) J. Neurocytol. 31:497.
3. Legg, J. et al. (2003) Development 130:6049.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSPG4