NG2/MCSP Antibody (JM10-13)

Western Blot: NG2/MCSP Antibody (JM10-13) [NBP2-66979] - Analysis of NG2 on SiHa cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: NG2/MCSP Antibody (JM10-13) [NBP2-66979] - Analysis of paraffin-embedded human malignant melanoma tissue using anti-NG2/MCSP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: NG2/MCSP Antibody (JM10-13) [NBP2-66979] - Analysis of SHG-44 cells with NG2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: NG2/MCSP Antibody (JM10-13) [NBP2-66979] - Analysis of paraffin-embedded mouse brain tissue using anti-NG2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: NG2/MCSP Antibody (JM10-13) [NBP2-66979] - Analysis of paraffin-embedded rat brain tissue using anti-NG2 antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clone: JM10-13
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

NG2/MCSP Antibody (JM10-13) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human NG2/MCSP aa 2,271-2,314 / 2,322. (SwissProt: Q6UVK1 Human; SwissProt: CSPG4 Mouse; SwissProt: Q00657 Rat)
• Localization: Cell membrane, Apical cell membrane, Cell projection, Cell surface.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CSPG4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:400
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for NG2/MCSP Antibody (JM10-13)

• AN2
• chondroitin sulfate proteoglycan 4 (melanoma-associated)
• chondroitin sulfate proteoglycan 4
• CSPG4
• EC 3.6.3
• HMW-MAA
• MCSP
• MCSPChondroitin sulfate proteoglycan NG2
• MCSPG
• MCSPGMelanoma chondroitin sulfate proteoglycan
• MEL-CSPG
• MSK16Melanoma-associated chondroitin sulfate proteoglycan
• NG2
• NG2EC 2.7.8

Background

Proteoglycans (PGs) are a family of proteins composed of different polypeptide chains containing glycosaminoglycan (GAG) modifications. They vary in their cellular locations and have diverse structure and functions. NG2 is a proteoglycan found in the nervous system, comprising a large integral membrane PG with a core protein of 300 kDa and at least one covalently attached chondroitin sulfate (CS) GAG chain. In the CNS this protein is expressed mainly on the surfaces of developing and adult oligodendrocyte precursor cells, but is also associated with developing chondrocytes, cardiomyocytes, pericytes and several human tumours.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NG2/MCSP Antibody (NBP2-66979)(1)

Publication using NBP2-66979

• Milan M, Maiullari F, Chirivì M et Al. Macrophages producing chondroitin sulfate proteoglycan-4 induce neuro-cardiac junction impairment in Duchenne muscular dystrophy J Pathol 2024-11-11 [PMID: 39523812]

Bioinformatics

• Gene Symbol: CSPG4