N-Cadherin Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

N-Cadherin Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human N-Cadherin Propeptide
  Ser26-Arg159
  Accession # P19022
• Specificity: Detects human N-Cadherin Propeptide in Western blots. In this format, less than 5% cross-reactivity with recombinant human (rh) N‑Cadherin extracellular domain, rhCadherin-8, rhCadherin-11, and rhR-Cadherin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CDH2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for N-Cadherin Antibody [Biotin]

• ACOGS
• ARVD14
• cadherin 2, type 1, N-cadherin (neuronal)
• Cadherin-2
• calcium-dependent adhesion protein, neuronal
• CD325 antigen
• CD325
• CDH2
• CDHN
• CDw325
• NCAD
• N-cadherin 1
• NCadherin
• N-Cadherin
• Neural cadherin
• neural-cadherin

Background

N‑Cadherin (Neural Cadherin; also CD325 and Cadherin-2) is a 130 ‑ 135 kDa member of the “classical” (or type I) cadherin subfamily, cadherin superfamily of proteins. It is expressed on multiple cell types, including neurons, fibroblasts, Schwann cells, endothelial cells and hepatic stellate cells. N‑Cadherin mediates homotypic binding, either in cis (same cell) or trans (adjacent cell). proN‑Cadherin is expressed as an 881 amino acid (aa) type I transmembrane glycoprotein. It may be initially inserted into the ER, where the 15 ‑ 20 kDa prodomain (aa 26 ‑ 159) is cleaved by proprotein convertase, and the mature molecule is transported to the surface. Alternatively, on neurons, proN‑Cadherin may first appear on the surface, with cleavage occurring at the time of synaptogenesis. Cleavage appears necessary for homophilic interaction as presence of the prodomain is suggested to negatively regulate oligomer formation. Over the entire prodomain, the human N‑Cadherin proregion shares 87% aa identity with the mouse N-Cadherin proregion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for N-Cadherin Antibody (BAF1388)(2)

Publications using BAF1388

• H Lee, M Choi, J Lim, M Jo, JY Han, TM Kim, Y Cho Magnetic Nanowire Networks for Dual-Isolation and Detection of Tumor-Associated Circulating Biomarkers Theranostics, 2018-01-01;8(2):505-517. 2018-01-01 [PMID: 29290823] (Functional Assay)
• Hisako Ishimine, Norio Yamakawa, Mari Sasao, Mika Tadokoro, Daisuke Kami, Shinji Komazaki et al. N-Cadherin is a prospective cell surface marker of human mesenchymal stem cells that have high ability for cardiomyocyte differentiation Biochemical and Biophysical Research Communications 2013-09-06 [PMID: 23899519]

Bioinformatics

• Gene Symbol: CDH2
• Uniprot:
  • P19022()